Restriction map for
pFN21AB3223
(Circular) (Six-base) MAPSORT of: pFN21AB3223.seq Check: 7789 from: 1 to: 6823
pFN21AB3223 6823 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4166 1937
Size: 2229 4594
Acc65I G'GTAC_C
Cuts at: 3119 4144 3119
Size: 1025 5798
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 2433 4886 6270 6538 1632
Size: 424 377 2453 1384 268 1917
Fragments arranged by size:
2453 1917 1384 424 377 268
AfeI AGC'GCT
Cuts at: 2526 2526
Size: 6823
AflII C'TTAA_G
Cuts at: 820 1017 4013 820
Size: 197 2996 3630
Fragments arranged by size:
3630 2996 197
AflIII A'CryG_T
Cuts at: 1203 2781 3841 5722 6699 1203
Size: 1578 1060 1881 977 1327
Fragments arranged by size:
1881 1578 1327 1060 977
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 3691 5552 3691
Size: 1861 4962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6823
AlwNI CAG_nnn'CTG
Cuts at: 4566 6138 4566
Size: 1572 5251
ApaLI G'TGCA_C
Cuts at: 4880 6036 4880
Size: 1156 5667
ApoI r'AATT_y
Cuts at: 1493 2602 2757 4132 4358 4510 6619 1493
Size: 1109 155 1375 226 152 2109 1697
Fragments arranged by size:
2109 1697 1375 1109 226 155 152
AseI AT'TA_AT
Cuts at: 160 1032 3855 5377 160
Size: 872 2823 1522 1606
Fragments arranged by size:
2823 1606 1522 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 4148 1870
Size: 73 2205 4545
Fragments arranged by size:
4545 2205 73
BamHI G'GATC_C
Cuts at: 4153 4520 4153
Size: 367 6456
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2560 3119 4144 5599 618
Size: 325 217 627 773 559 1025 1455 1842
Fragments arranged by size:
1842 1455 1025 773 627 559 325 217
BanII G_rGCy'C
Cuts at: 1391 2284 2432 4142 1391
Size: 893 148 1710 4072
Fragments arranged by size:
4072 1710 893 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2867 3495 928
Size: 971 968 628 4256
Fragments arranged by size:
4256 971 968 628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4715 5925 4715
Size: 1210 5613
BclI T'GATC_A
Cuts at: 1548 2254 1548
Size: 706 6117
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4049 5434 136
Size: 107 122 71 631 605 2377 1385 1525
Fragments arranged by size:
2377 1525 1385 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2413 6818 2413
Size: 4405 2418
BlpI GC'TnA_GC
Cuts at: 2054 4240 2054
Size: 2186 4637
Bme1580I G_kGCm'C
Cuts at: 1790 4884 6040 1790
Size: 3094 1156 2573
Fragments arranged by size:
3094 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2135 4554 5512
Size: 416 53 83 399 24 718 2419 958
Cuts at: 5512 442
Size: 1753
Fragments arranged by size:
2419 1753 958 718 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2673 1056
Size: 1617 5206
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2853 2365
Size: 488 6335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3162 5483 1239
Size: 1923 2321 2579
Fragments arranged by size:
2579 2321 1923
Bpu10I CC'TnA_GC
Cuts at: 2646 2646
Size: 6823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2848 4948 5813 6111 2848
Size: 2100 865 298 3560
Fragments arranged by size:
3560 2100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2497 5486 882
Size: 617 998 2989 2219
Fragments arranged by size:
2989 2219 998 617
BsaAI yAC'GTr
Cuts at: 493 1204 4614 493
Size: 711 3410 2702
Fragments arranged by size:
3410 2702 711
BsaBI GATnn'nnATC
Cuts at: 6817 6817
Size: 6823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4630 5012 5647 275
Size: 53 83 186 936 3097 382 635 1451
Fragments arranged by size:
3097 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5256 5654 5928 6075 6486 5256
Size: 398 274 147 411 5593
Fragments arranged by size:
5593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6598
Fragments arranged by size:
6598 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2181 2515 2934 1186
Size: 995 334 419 5075
Fragments arranged by size:
5075 995 419 334
BseYI C'CCAG_C
Cuts at: 2013 2431 6026 6522 2013
Size: 418 3595 496 2314
Fragments arranged by size:
3595 2314 496 418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4273 5034 6062 1540
Size: 275 186 2272 761 1028 2301
Fragments arranged by size:
2301 2272 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4884 4969 6040 4884
Size: 85 1071 5667
Fragments arranged by size:
5667 1071 85
BsmI GAATG_Cn'
Cuts at: 2967 2992 3863 4334 4427 2967
Size: 25 871 471 93 5363
Fragments arranged by size:
5363 871 471 93 25
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2432 4142 4884 4969 6040 1391
Size: 399 494 148 1710 742 85 1071 2174
Fragments arranged by size:
2174 1710 1071 742 494 399 148 85
BspEI T'CCGG_A
Cuts at: 6486 6486
Size: 6823
BspHI T'CATG_A
Cuts at: 2310 3664 4712 6442 2310
Size: 1354 1048 1730 2691
Fragments arranged by size:
2691 1730 1354 1048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4178 844
Size: 3334 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4710 1394
Size: 429 2887 3507
Fragments arranged by size:
3507 2887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3243 5318 5492 65
Size: 1183 1995 2075 174 1396
Fragments arranged by size:
2075 1995 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5467 5654 1700
Size: 110 3657 187 2869
Fragments arranged by size:
3657 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2361 96
Size: 2265 4558
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 2869 3218 4883 5895 2869
Size: 349 1665 1012 3797
Fragments arranged by size:
3797 1665 1012 349
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6823
BstBI TT'CG_AA
Cuts at: 5627 5627
Size: 6823
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6823
BstXI CCAn_nnnn'nTGG
Cuts at: 4111 4111
Size: 6823
BstYI r'GATC_y
Cuts at: 1972 2413 4153 4520 4905 4922 6374 6460 6472
Size: 441 1740 367 385 17 1452 86 12
Cuts at: 6472 6818 1972
Size: 346 1977
Fragments arranged by size:
1977 1740 1452 441 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3945 513
Size: 552 2880 3391
Fragments arranged by size:
3391 2880 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2093 2882 487
Size: 683 56 867 789 4428
Fragments arranged by size:
4428 867 789 683 56
BtsI GCAGTG_nn'
Cuts at: 4067 4339 5133 5153 4067
Size: 272 794 20 5737
Fragments arranged by size:
5737 794 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6788
DraI TTT'AAA
Cuts at: 4127 4127
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 3131 3877
Size: 54 1425 50 275 18 198 1103 746
Cuts at: 3877 4270 5159 6788 8
Size: 393 889 1629 43
Fragments arranged by size:
1629 1425 1103 889 746 393 275 198
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4270 1537
Size: 275 2458 4090
Fragments arranged by size:
4090 2458 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3896 4753 1353
Size: 2543 857 3423
Fragments arranged by size:
3423 2543 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3826 5410 5784 5930 387
Size: 1064 138 2237 1584 374 146 1280
Fragments arranged by size:
2237 1584 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2433 3162 4886 5483 6270 6538
Size: 393 424 377 729 1724 597 787 268
Cuts at: 6538 1239
Size: 1524
Fragments arranged by size:
1724 1524 787 729 597 424 393 377
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3114 3114
Size: 6823
EcoO109I rG'GnC_Cy
Cuts at: 2694 2766 3335 2694
Size: 72 569 6182
Fragments arranged by size:
6182 569 72
EcoRI G'AATT_C
Cuts at: 2757 4132 4510 2757
Size: 1375 378 5070
Fragments arranged by size:
5070 1375 378
EcoRV GAT'ATC
Cuts at: 3721 3721
Size: 6823
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2528 5970 1678
Size: 850 3442 2531
Fragments arranged by size:
3442 2531 850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 3224 3256 3677
Size: 32 135 32 826 32 794 32 421
Cuts at: 3677 3689 3692 3709 3721 3724 5464 5496 5538
Size: 12 3 17 12 3 1740 32 42
Cuts at: 5538 5570 1373
Size: 32 2626
Fragments arranged by size:
2626 1740 826 794 421 135 42 32
32 32 32 32 32 17 12 12
3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2198 4167 4413 669
Size: 988 281 260 1969 246 3079
Fragments arranged by size:
3079 1969 988 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KpnI G_GTAC'C
Cuts at: 3123 4148 3123
Size: 1025 5798
MfeI C'AATT_G
Cuts at: 3405 4422 3405
Size: 1017 5806
MluI A'CGCG_T
Cuts at: 3841 6699 3841
Size: 2858 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3076 3454 5937 6121 1305
Size: 1771 378 2483 184 2007
Fragments arranged by size:
2483 2007 1771 378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3133 10
Size: 54 1425 343 1301 3700
Fragments arranged by size:
3700 1425 1301 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2250 2262 2817 4781 5140 5299 518
Size: 648 1084 12 555 1964 359 159 2042
Fragments arranged by size:
2042 1964 1084 648 555 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 4239 4916 6064 6309 1606
Size: 2633 677 1148 245 2120
Fragments arranged by size:
2633 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6271
NdeI CA'TA_TG
Cuts at: 387 4119 387
Size: 3732 3091
NheI G'CTAG_C
Cuts at: 1052 2669 1052
Size: 1617 5206
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 2785 4181 5726 1132
Size: 97 1556 1396 1545 2229
Fragments arranged by size:
2229 1556 1545 1396 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6823
PciI A'CATG_T
Cuts at: 2781 5722 2781
Size: 2941 3882
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3129 3140 3331 1108
Size: 2021 11 191 4600
Fragments arranged by size:
4600 2021 191 11
PfoI T'CCnGG_A
Cuts at: 2495 2495
Size: 6823
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2694 2766 3335 2694
Size: 72 569 6182
Fragments arranged by size:
6182 569 72
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2899 2931 3601 3633 3736 3768 2899
Size: 32 670 32 103 32 5954
Fragments arranged by size:
5954 670 103 32 32 32
PstI C_TGCA'G
Cuts at: 830 2524 4175 830
Size: 1694 1651 3478
Fragments arranged by size:
3478 1694 1651
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SanDI GG'GwC_CC
Cuts at: 2694 2694
Size: 6823
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 3754 5071 6783 3754
Size: 1317 1712 3794
Fragments arranged by size:
3794 1712 1317
SfcI C'TryA_G
Cuts at: 826 1045 2034 2520 4171 5306 5987 6178 826
Size: 219 989 486 1651 1135 681 191 1471
Fragments arranged by size:
1651 1471 1135 989 681 486 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 4150 1872
Size: 2278 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2827 4013 4927 5828 6090 820
Size: 197 926 884 1186 914 901 262 1553
Fragments arranged by size:
1553 1186 926 914 901 884 262 197
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 4747 5
Size: 47 4695 2081
Fragments arranged by size:
4695 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6823
StyI C'CwwG_G
Cuts at: 513 1065 4262 513
Size: 552 3197 3074
Fragments arranged by size:
3197 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2057 4850 4867 5020 5205 5650 325
Size: 1255 477 2793 17 153 185 445 1498
Fragments arranged by size:
2793 1498 1255 477 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2361 3530 3752
Size: 274 80 33 51 1443 384 1169 222
Cuts at: 3752 5069 6781 96
Size: 1317 1712 138
Fragments arranged by size:
1712 1443 1317 1169 384 274 222 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 6783 6815 2860
Size: 32 3891 32 2868
Fragments arranged by size:
3891 2868 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3494 1316
Size: 2178 4645
XbaI T'CTAG_A
Cuts at: 2222 3734 3964 4159 2222
Size: 1512 230 195 4886
Fragments arranged by size:
4886 1512 230 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2747 2747
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 4148 1870
Size: 2278 4545
XmnI GAAnn'nnTTC
Cuts at: 3660 4952 3660
Size: 1292 5531
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI