Restriction map for
pFN21AB3222
(Circular) (Six-base) MAPSORT of: pFN21AB3222.seq Check: 6322 from: 1 to: 7642
pFN21AB3222 7642 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4985 1937
Size: 3048 4594
Acc65I G'GTAC_C
Cuts at: 2040 4963 2040
Size: 2923 4719
AclI AA'CG_TT
Cuts at: 1563 3639 5769 6142 1563
Size: 2076 2130 373 3063
Fragments arranged by size:
3063 2130 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3175 4490 4511 4646 5705 7089 7357 1632
Size: 1543 1315 21 135 1059 1384 268 1917
Fragments arranged by size:
1917 1543 1384 1315 1059 268 135 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 2302 2588 6541 7518 1203
Size: 1099 286 3953 977 1327
Fragments arranged by size:
3953 1327 1099 977 286
AgeI A'CCGG_T
Cuts at: 6473 6473
Size: 7642
AhdI GACnn_n'nnGTC
Cuts at: 2734 6371 2734
Size: 3637 4005
AleI CACnn'nnGTG
Cuts at: 1166 2669 3326 1166
Size: 1503 657 5482
Fragments arranged by size:
5482 1503 657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7642
AlwNI CAG_nnn'CTG
Cuts at: 4402 4744 4840 5385 6957 4402
Size: 342 96 545 1572 5087
Fragments arranged by size:
5087 1572 545 342 96
ApaLI G'TGCA_C
Cuts at: 2660 3259 5699 6855 2660
Size: 599 2440 1156 3447
Fragments arranged by size:
3447 2440 1156 599
ApoI r'AATT_y
Cuts at: 1493 2075 3704 4510 4951 5177 5329 7438 1493
Size: 582 1629 806 441 226 152 2109 1697
Fragments arranged by size:
2109 1697 1629 806 582 441 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6196 160
Size: 872 5164 1606
Fragments arranged by size:
5164 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 3029 3363 4967 1870
Size: 73 185 901 334 1604 4545
Fragments arranged by size:
4545 1604 901 334 185 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2354 2387 2354
Size: 33 7609
BamHI G'GATC_C
Cuts at: 4972 5339 4972
Size: 367 7275
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2930 4963 6418 618
Size: 325 217 627 253 890 2033 1455 1842
Fragments arranged by size:
2033 1842 1455 890 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2600 3411 4961 1391
Size: 1209 811 1550 4072
Fragments arranged by size:
4072 1550 1209 811
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2340 2466 3328 928
Size: 971 441 126 862 5242
Fragments arranged by size:
5242 971 862 441 126
BbvCI CC'TCA_GC
Cuts at: 4385 4385
Size: 7642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2289 2323 4585 4619 5833 5867 1903
Size: 34 352 34 2262 34 1214 34 3678
Fragments arranged by size:
3678 2262 1214 352 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5534 6744 5534
Size: 1210 6432
BclI T'GATC_A
Cuts at: 1548 2829 1548
Size: 1281 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6253 136
Size: 107 122 71 631 605 4581 1525
Fragments arranged by size:
4581 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3801 7637 3801
Size: 3836 3806
BlpI GC'TnA_GC
Cuts at: 4754 5059 4754
Size: 305 7337
Bme1580I G_kGCm'C
Cuts at: 1790 2664 3263 5703 6859 1790
Size: 874 599 2440 1156 2573
Fragments arranged by size:
2573 2440 1156 874 599
BmgBI CAC'GTC
Cuts at: 1318 2589 3676 4695 1318
Size: 1271 1087 1019 4265
Fragments arranged by size:
4265 1271 1087 1019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2740 5373 6331
Size: 416 53 83 399 24 1323 2633 958
Cuts at: 6331 442
Size: 1753
Fragments arranged by size:
2633 1753 1323 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3787 3011
Size: 776 6866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2075 2387 2872 3089 3842 4283 4717
Size: 827 9 312 485 217 753 441 434
Cuts at: 4717 6302 1239
Size: 1585 2579
Fragments arranged by size:
2579 1585 827 753 485 441 434 312
217 9
Bpu10I CC'TnA_GC
Cuts at: 2999 4385 4911 2999
Size: 1386 526 5730
Fragments arranged by size:
5730 1386 526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5767 6632 6930 5767
Size: 865 298 6479
Fragments arranged by size:
6479 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2241 3049 6305 882
Size: 617 742 808 3256 2219
Fragments arranged by size:
3256 2219 808 742 617
BsaAI yAC'GTr
Cuts at: 493 1204 2303 2434 5433 493
Size: 711 1099 131 2999 2702
Fragments arranged by size:
2999 2702 1099 711 131
BsaBI GATnn'nnATC
Cuts at: 3965 7636 3965
Size: 3671 3971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2735 5449 5831 6466
Size: 53 83 186 936 1202 2714 382 635
Cuts at: 6466 275
Size: 1451
Fragments arranged by size:
2714 1451 1202 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2535 6075 6473 6747 6894 7305 2535
Size: 3540 398 274 147 411 2872
Fragments arranged by size:
3540 2872 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2612 2642 4025
Size: 30 165 30 786 30 393 30 1383
Cuts at: 4025 4055 1178
Size: 30 4765
Fragments arranged by size:
4765 1383 786 393 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2363 2827 3473 3935 4094 4097 4126
Size: 1024 153 464 646 462 159 3 29
Cuts at: 4126 1186
Size: 4702
Fragments arranged by size:
4702 1024 646 464 462 159 153 29
3
BseYI C'CCAG_C
Cuts at: 3665 3878 4707 6845 7341 3665
Size: 213 829 2138 496 3966
Fragments arranged by size:
3966 2138 829 496 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2944 4136 1163
Size: 573 1208 1192 4669
Fragments arranged by size:
4669 1208 1192 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5092 5853 6881 1540
Size: 275 186 3091 761 1028 2301
Fragments arranged by size:
3091 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2535 2664 2946 3263 3384 4220 5703 5788 6859
Size: 129 282 317 121 836 1483 85 1071
Cuts at: 6859 2535
Size: 3318
Fragments arranged by size:
3318 1483 1071 836 317 282 129 121
85
BsmI GAATG_Cn'
Cuts at: 2473 2647 5153 5246 2473
Size: 174 2506 93 4869
Fragments arranged by size:
4869 2506 174 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2525 3666 2525
Size: 1141 6501
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 2600 2664 2946 3263 3384 3411
Size: 399 745 65 64 282 317 121 27
Cuts at: 3411 4220 4961 5703 5788 6859 1391
Size: 809 741 742 85 1071 2174
Fragments arranged by size:
2174 1071 809 745 742 741 399 317
282 121 85 65 64 27
BspEI T'CCGG_A
Cuts at: 7305 7305
Size: 7642
BspHI T'CATG_A
Cuts at: 2832 4791 5531 7261 2832
Size: 1959 740 1730 3213
Fragments arranged by size:
3213 1959 1730 740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3615 4596 4997 844
Size: 2771 981 401 3489
Fragments arranged by size:
3489 2771 981 401
BsrBI CCG'CTC
Cuts at: 1394 1823 2956 2983 5529 1394
Size: 429 1133 27 2546 3507
Fragments arranged by size:
3507 2546 1133 429 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 6137 6311 65
Size: 1183 4889 174 1396
Fragments arranged by size:
4889 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6286 6473 1700
Size: 110 4476 187 2869
Fragments arranged by size:
4476 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7642
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7642
BssSI C'ACGA_G
Cuts at: 3052 5702 6714 3052
Size: 2650 1012 3980
Fragments arranged by size:
3980 2650 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2025 3085 794
Size: 1231 1060 5351
Fragments arranged by size:
5351 1231 1060
BstBI TT'CG_AA
Cuts at: 2309 3708 6446 2309
Size: 1399 2738 3505
Fragments arranged by size:
3505 2738 1399
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7642
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2044 2617 3886 3985 4307 2011
Size: 33 573 1269 99 322 5346
Fragments arranged by size:
5346 1269 573 322 99 33
BstYI r'GATC_y
Cuts at: 1972 2161 2223 3111 3801 3870 4711 4972 5339
Size: 189 62 888 690 69 841 261 367
Cuts at: 5339 5724 5741 7193 7279 7291 7637 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 888 841 690 385 367 346
261 189 86 69 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 3470 3512 3470
Size: 42 7600
BtgI C'CryG_G
Cuts at: 513 1065 2211 2667 3324 3942 513
Size: 552 1146 456 657 618 4213
Fragments arranged by size:
4213 1146 657 618 552 456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6903
Fragments arranged by size:
6903 683 56
BtsI GCAGTG_nn'
Cuts at: 4124 4699 5158 5952 5972 4124
Size: 575 459 794 20 5794
Fragments arranged by size:
5794 794 575 459 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7607
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DraIII CAC_nnn'GTG
Cuts at: 2668 3228 2668
Size: 560 7082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3680 4787 6649 809
Size: 507 2364 1107 1862 1802
Fragments arranged by size:
2364 1862 1802 1107 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2214 2568
Size: 54 1425 50 275 18 204 180 354
Cuts at: 2568 2709 5089 5978 7607 8
Size: 141 2380 889 1629 43
Fragments arranged by size:
2380 1629 1425 889 354 275 204 180
141 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 2903 3224 3609 4071 5572 1353
Size: 987 563 321 385 462 1501 3423
Fragments arranged by size:
3423 1501 987 563 462 385 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3509 6229 6603 6749 387
Size: 1064 138 1920 2720 374 146 1280
Fragments arranged by size:
2720 1920 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2075 2387 2872 3089 3175 3842
Size: 393 434 9 312 485 217 86 667
Cuts at: 3842 4283 4490 4511 4646 4717 5705 6302 7089
Size: 441 207 21 135 71 988 597 787
Cuts at: 7089 7357 1239
Size: 268 1524
Fragments arranged by size:
1524 988 787 667 597 485 441 434
393 312 268 217 207 135 86 71
21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3455 3455
Size: 7642
EcoO109I rG'GnC_Cy
Cuts at: 2050 2824 3214 3768 4198 4267 2050
Size: 774 390 554 430 69 5425
Fragments arranged by size:
5425 774 554 430 390 69
EcoRI G'AATT_C
Cuts at: 4510 4951 5329 4510
Size: 441 378 6823
Fragments arranged by size:
6823 441 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 7642
FspI TGC'GCA
Cuts at: 6148 6148
Size: 7642
HaeII r_GCGC'y
Cuts at: 1678 4342 6789 1678
Size: 2664 2447 2531
Fragments arranged by size:
2664 2531 2447
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2505 2537 2720 2752 4025
Size: 32 135 32 933 32 183 32 1273
Cuts at: 4025 4057 6283 6315 6357 6389 1373
Size: 32 2226 32 42 32 2626
Fragments arranged by size:
2626 2226 1273 933 183 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4986 5232 669
Size: 988 281 3048 246 3079
Fragments arranged by size:
3079 3048 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3659 4658 748
Size: 2911 999 3732
Fragments arranged by size:
3732 2911 999
HpaI GTT'AAC
Cuts at: 5232 5232
Size: 7642
KpnI G_GTAC'C
Cuts at: 2044 4967 2044
Size: 2923 4719
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2706 3489 4650 6756 6940 1305
Size: 1401 783 1161 2106 184 2007
Fragments arranged by size:
2106 2007 1401 1161 783 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2570 2711 10
Size: 54 1425 343 204 534 141 4941
Fragments arranged by size:
4941 1425 534 343 204 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2174 2216 2615 2669 3326 3569
Size: 648 843 165 42 399 54 657 243
Cuts at: 3569 3983 5600 5959 6118 518
Size: 414 1617 359 159 2042
Fragments arranged by size:
2042 1617 843 657 648 414 399 359
243 165 159 54 42
MspA1I CmG'CkG
Cuts at: 1606 2025 3326 3886 5058 5735 6883 7128 1606
Size: 419 1301 560 1172 677 1148 245 2120
Fragments arranged by size:
2120 1301 1172 1148 677 560 419 245
NcoI C'CATG_G
Cuts at: 513 1065 2211 3942 513
Size: 552 1146 1731 4213
Fragments arranged by size:
4213 1731 1146 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7642
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 3107 4066 5000 6545 1132
Size: 97 1878 959 934 1545 2229
Fragments arranged by size:
2229 1878 1545 959 934 97
PasI CC'CwG_GG
Cuts at: 1348 4045 4271 1348
Size: 2697 226 4719
Fragments arranged by size:
4719 2697 226
PciI A'CATG_T
Cuts at: 6541 6541
Size: 7642
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4402 1108
Size: 3294 4348
PfoI T'CCnGG_A
Cuts at: 3579 3579
Size: 7642
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PmlI CAC'GTG
Cuts at: 2303 2303
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2824 3214 3768 4267 2050
Size: 774 390 554 499 5425
Fragments arranged by size:
5425 774 554 499 390
PshAI GACnn'nnGTC
Cuts at: 1613 4922 1613
Size: 3309 4333
PsiI TTA'TAA
Cuts at: 5212 5212
Size: 7642
PstI C_TGCA'G
Cuts at: 830 3081 3360 3427 3489 3870 4836 4994 830
Size: 2251 279 67 62 381 966 158 3478
Fragments arranged by size:
3478 2251 966 381 279 158 67 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 3886 3886
Size: 7642
SacII CC_GC'GG
Cuts at: 3327 3327
Size: 7642
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SanDI GG'GwC_CC
Cuts at: 4267 4267
Size: 7642
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 3146 3158 5890 7602 3146
Size: 12 2732 1712 3186
Fragments arranged by size:
3186 2732 1712 12
SfcI C'TryA_G
Cuts at: 826 1045 3077 3356 3423 3485 3556 3866 4832
Size: 219 2032 279 67 62 71 310 966
Cuts at: 4832 4990 6125 6806 6997 826
Size: 158 1135 681 191 1471
Fragments arranged by size:
2032 1471 1135 966 681 310 279 219
191 158 71 67 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7642
SmaI CCC'GGG
Cuts at: 1872 3365 4969 1872
Size: 1493 1604 4545
Fragments arranged by size:
4545 1604 1493
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5746 6647 6909 820
Size: 197 926 3803 901 262 1553
Fragments arranged by size:
3803 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 5000 1132
Size: 3868 3774
SspI AAT'ATT
Cuts at: 5 52 5566 5
Size: 47 5514 2081
Fragments arranged by size:
5514 2081 47
StuI AGG'CCT
Cuts at: 1517 3035 1517
Size: 1518 6124
StyI C'CwwG_G
Cuts at: 513 1065 2211 3942 4068 4377 5081 513
Size: 552 1146 1731 126 309 704 3074
Fragments arranged by size:
3074 1731 1146 704 552 309 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 2875 5669 5686 5839 6024 6469
Size: 1255 888 407 2794 17 153 185 445
Cuts at: 6469 325
Size: 1498
Fragments arranged by size:
2794 1498 1255 888 445 407 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 3144 3156
Size: 274 80 33 51 1443 444 723 12
Cuts at: 3156 3600 5888 7600 96
Size: 444 2288 1712 138
Fragments arranged by size:
2288 1712 1443 723 444 444 274 138
80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 4166 4198 7602 7634 2189
Size: 32 1945 32 3404 32 2197
Fragments arranged by size:
3404 2197 1945 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2517 1316
Size: 1201 6441
XbaI T'CTAG_A
Cuts at: 4978 4978
Size: 7642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3058 3058
Size: 7642
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 3363 4967 1870
Size: 1493 1604 4545
Fragments arranged by size:
4545 1604 1493
XmnI GAAnn'nnTTC
Cuts at: 3524 4893 5771 3524
Size: 1369 878 5395
Fragments arranged by size:
5395 1369 878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI