Restriction map for
pFN21AB3218
(Circular) (Six-base) MAPSORT of: pFN21AB3218.seq Check: 2830 from: 1 to: 7393
pFN21AB3218 7393 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5203 278
Size: 53 83 186 936 3667 2468
Fragments arranged by size:
3667 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 3373 3813 4736 1937
Size: 643 793 440 923 4594
Fragments arranged by size:
4594 923 793 643 440
Acc65I G'GTAC_C
Cuts at: 4714 4714
Size: 7393
AclI AA'CG_TT
Cuts at: 1563 4064 5520 5893 1563
Size: 2501 1456 373 3063
Fragments arranged by size:
3063 2501 1456 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2403 3422 3964 4168 4525 4578 5456 6840
Size: 771 1019 542 204 357 53 878 1384
Cuts at: 6840 7108 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1019 878 771 542 357 268
204 53
AflII C'TTAA_G
Cuts at: 820 1017 3071 820
Size: 197 2054 5142
Fragments arranged by size:
5142 2054 197
AflIII A'CryG_T
Cuts at: 1203 3628 6292 7269 1203
Size: 2425 2664 977 1327
Fragments arranged by size:
2664 2425 1327 977
AgeI A'CCGG_T
Cuts at: 6224 6224
Size: 7393
AhdI GACnn_n'nnGTC
Cuts at: 6122 6122
Size: 7393
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4632 1184
Size: 3448 3945
AlwNI CAG_nnn'CTG
Cuts at: 5136 6708 5136
Size: 1572 5821
ApaLI G'TGCA_C
Cuts at: 2586 5450 6606 2586
Size: 2864 1156 3373
Fragments arranged by size:
3373 2864 1156
ApoI r'AATT_y
Cuts at: 1493 2219 4702 4928 5080 7189 1493
Size: 726 2483 226 152 2109 1697
Fragments arranged by size:
2483 2109 1697 726 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5947 160
Size: 872 4915 1606
Fragments arranged by size:
4915 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7393
AvaI C'yCGr_G
Cuts at: 1870 1943 4718 1870
Size: 73 2775 4545
Fragments arranged by size:
4545 2775 73
BamHI G'GATC_C
Cuts at: 4723 5090 4723
Size: 367 7026
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 4714 6169 618
Size: 325 217 627 845 2082 1455 1842
Fragments arranged by size:
2082 1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2364 3663 4241 4712 1391
Size: 973 1299 578 471 4072
Fragments arranged by size:
4072 1299 973 578 471
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4650 928
Size: 971 2751 3671
Fragments arranged by size:
3671 2751 971
BbvCI CC'TCA_GC
Cuts at: 3264 3590 3264
Size: 326 7067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5584 5618 1903
Size: 34 3647 34 3678
Fragments arranged by size:
3678 3647 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2213 2674 5285 6495 2213
Size: 461 2611 1210 3111
Fragments arranged by size:
3111 2611 1210 461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7393
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6004 136
Size: 107 122 71 631 605 4332 1525
Fragments arranged by size:
4332 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3321 7388 3321
Size: 4067 3326
BlpI GC'TnA_GC
Cuts at: 2358 3365 4810 2358
Size: 1007 1445 4941
Fragments arranged by size:
4941 1445 1007
Bme1580I G_kGCm'C
Cuts at: 1790 2254 2590 5454 6610 1790
Size: 464 336 2864 1156 2573
Fragments arranged by size:
2864 2573 1156 464 336
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7393
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 5124 6082
Size: 416 53 83 399 24 1003 2704 958
Cuts at: 6082 442
Size: 1753
Fragments arranged by size:
2704 1753 1003 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2607 2612 3248 3663 6053 1239
Size: 1368 5 636 415 2390 2579
Fragments arranged by size:
2579 2390 1368 636 415 5
Bpu10I CC'TnA_GC
Cuts at: 3264 3590 3264
Size: 326 7067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3224 3802 5518 6383 6681 3224
Size: 578 1716 865 298 3936
Fragments arranged by size:
3936 1716 865 578 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6056 882
Size: 617 4557 2219
Fragments arranged by size:
4557 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5184 493
Size: 711 3980 2702
Fragments arranged by size:
3980 2702 711
BsaBI GATnn'nnATC
Cuts at: 7387 7387
Size: 7393
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5200 5582 6217 275
Size: 53 83 186 936 3667 382 635 1451
Fragments arranged by size:
3667 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5826 6224 6498 6645 7056 5826
Size: 398 274 147 411 6163
Fragments arranged by size:
6163 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3177 3207 1178
Size: 30 165 30 1774 30 5364
Fragments arranged by size:
5364 1774 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2892 3245 3281 3413 1186
Size: 1706 353 36 132 5166
Fragments arranged by size:
5166 1706 353 132 36
BseYI C'CCAG_C
Cuts at: 2019 3222 4309 6596 7092 2019
Size: 1203 1087 2287 496 2320
Fragments arranged by size:
2320 2287 1203 1087 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3559 4050 1163
Size: 573 1823 491 4506
Fragments arranged by size:
4506 1823 573 491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4843 5604 6632 1540
Size: 275 186 2842 761 1028 2301
Fragments arranged by size:
2842 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 2590 4241 5454 5539 6610 2364
Size: 226 1651 1213 85 1071 3147
Fragments arranged by size:
3147 1651 1213 1071 226 85
BsmI GAATG_Cn'
Cuts at: 2544 3555 4904 4997 2544
Size: 1011 1349 93 4940
Fragments arranged by size:
4940 1349 1011 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2254 2364 2590 3663 4241 4712 5454
Size: 399 464 110 226 1073 578 471 742
Cuts at: 5454 5539 6610 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1073 1071 742 578 471 464 399
226 110 85
BspEI T'CCGG_A
Cuts at: 7056 7056
Size: 7393
BspHI T'CATG_A
Cuts at: 2314 5282 7012 2314
Size: 2968 1730 2695
Fragments arranged by size:
2968 2695 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3172 4748 844
Size: 2328 1576 3489
Fragments arranged by size:
3489 2328 1576
BsrBI CCG'CTC
Cuts at: 1394 1823 5280 1394
Size: 429 3457 3507
Fragments arranged by size:
3507 3457 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5888 6062 65
Size: 1183 4640 174 1396
Fragments arranged by size:
4640 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6037 6224 1700
Size: 110 4227 187 2869
Fragments arranged by size:
4227 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7393
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7393
BssSI C'ACGA_G
Cuts at: 5453 6465 5453
Size: 1012 6381
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7393
BstBI TT'CG_AA
Cuts at: 6197 6197
Size: 7393
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7393
BstXI CCAn_nnnn'nTGG
Cuts at: 2575 2575
Size: 7393
BstYI r'GATC_y
Cuts at: 1972 3118 3321 3799 3955 4213 4370 4459 4723
Size: 1146 203 478 156 258 157 89 264
Cuts at: 4723 5090 5475 5492 6944 7030 7042 7388 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1146 478 385 367 346 264
258 203 157 156 89 86 17 12
BstZ17I GTA'TAC
Cuts at: 2581 2581
Size: 7393
BtgI C'CryG_G
Cuts at: 513 1065 2004 2366 513
Size: 552 939 362 5540
Fragments arranged by size:
5540 939 552 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6654
Fragments arranged by size:
6654 683 56
BtsI GCAGTG_nn'
Cuts at: 3610 3892 4313 4909 5703 5723 3610
Size: 282 421 596 794 20 5280
Fragments arranged by size:
5280 794 596 421 282 20
ClaI AT'CG_AT
Cuts at: 1554 3921 1554
Size: 2367 5026
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7358
DraI TTT'AAA
Cuts at: 4405 4697 4405
Size: 292 7101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6400 809
Size: 507 5084 1802
Fragments arranged by size:
5084 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4840 5729 7358
Size: 54 1425 50 275 18 3010 889 1629
Cuts at: 7358 8
Size: 43
Fragments arranged by size:
3010 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4840 1537
Size: 275 3028 4090
Fragments arranged by size:
4090 3028 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2497 3289 3354 3441 4599 5323 1353
Size: 1144 792 65 87 1158 724 3423
Fragments arranged by size:
3423 1158 1144 792 724 87 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5980 6354 6500 387
Size: 1064 138 4391 374 146 1280
Fragments arranged by size:
4391 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 4239 2362
Size: 1877 5516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2607 2612 3248 3422 3663 3964
Size: 393 771 204 5 636 174 241 301
Cuts at: 3964 4168 4525 4578 5456 6053 6840 7108 1239
Size: 204 357 53 878 597 787 268 1524
Fragments arranged by size:
1524 878 787 771 636 597 393 357
301 268 241 204 204 174 53 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3945 4431 3945
Size: 486 6907
EcoO109I rG'GnC_Cy
Cuts at: 3067 3067
Size: 7393
EcoRI G'AATT_C
Cuts at: 2219 4702 5080 2219
Size: 2483 378 4532
Fragments arranged by size:
4532 2483 378
EcoRV GAT'ATC
Cuts at: 2494 4683 2494
Size: 2189 5204
FspI TGC'GCA
Cuts at: 5899 5899
Size: 7393
HaeII r_GCGC'y
Cuts at: 1678 6540 1678
Size: 4862 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2730 2762 3271 3303 4268
Size: 32 135 32 1158 32 509 32 965
Cuts at: 4268 4300 4318 4350 6034 6066 6108 6140 1373
Size: 32 18 32 1684 32 42 32 2626
Fragments arranged by size:
2626 1684 1158 965 509 135 42 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3034 4204 4737 4983 669
Size: 988 281 1096 1170 533 246 3079
Fragments arranged by size:
3079 1170 1096 988 533 281 246
HindIII A'AGCT_T
Cuts at: 748 2831 748
Size: 2083 5310
HpaI GTT'AAC
Cuts at: 4983 4983
Size: 7393
KpnI G_GTAC'C
Cuts at: 4718 4718
Size: 7393
MfeI C'AATT_G
Cuts at: 4992 4992
Size: 7393
MluI A'CGCG_T
Cuts at: 7269 7269
Size: 7393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3408 4361 6507 6691 1305
Size: 2103 953 2146 184 2007
Fragments arranged by size:
2146 2103 2007 953 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5571
Fragments arranged by size:
5571 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5351 5710 5869 518
Size: 648 4185 359 159 2042
Fragments arranged by size:
4185 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2357 3226 3743 4809 5486 6634 6879 1606
Size: 751 869 517 1066 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 869 751 677 517 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2366 513
Size: 552 939 362 5540
Fragments arranged by size:
5540 939 552 362
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7393
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7393
NotI GC'GGCC_GC
Cuts at: 4840 4840
Size: 7393
NspI r_CATG'y
Cuts at: 1132 1229 2250 3632 4751 6296 1132
Size: 97 1021 1382 1119 1545 2229
Fragments arranged by size:
2229 1545 1382 1119 1021 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7393
PciI A'CATG_T
Cuts at: 3628 6292 3628
Size: 2664 4729
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2432 3932 1108
Size: 1324 1500 4569
Fragments arranged by size:
4569 1500 1324
PmeI GTTT'AAAC
Cuts at: 4697 4697
Size: 7393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5462 5494 6975 7007 5462
Size: 32 1481 32 5848
Fragments arranged by size:
5848 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3067 3067
Size: 7393
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7393
PsiI TTA'TAA
Cuts at: 4963 4963
Size: 7393
PstI C_TGCA'G
Cuts at: 830 2356 2674 4745 830
Size: 1526 318 2071 3478
Fragments arranged by size:
3478 2071 1526 318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7393
PvuII CAG'CTG
Cuts at: 2357 3226 3743 2357
Size: 869 517 6007
Fragments arranged by size:
6007 869 517
SacI G_AGCT'C
Cuts at: 2364 4241 2364
Size: 1877 5516
SalI G'TCGA_C
Cuts at: 1936 4735 1936
Size: 2799 4594
SapI GCTCTTCn'nnn_
Cuts at: 3354 4599 3354
Size: 1245 6148
SbfI CC_TGCA'GG
Cuts at: 4745 4745
Size: 7393
ScaI AGT'ACT
Cuts at: 5641 7353 5641
Size: 1712 5681
SfcI C'TryA_G
Cuts at: 826 1045 2071 2352 2670 3038 3164 3374 3573
Size: 219 1026 281 318 368 126 210 199
Cuts at: 3573 3738 4741 5876 6557 6748 826
Size: 165 1003 1135 681 191 1471
Fragments arranged by size:
1471 1135 1026 1003 681 368 318 281
219 210 199 191 165 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7393
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7393
SmaI CCC'GGG
Cuts at: 1872 4720 1872
Size: 2848 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3071 3239 3817 5497 6398 6660
Size: 197 926 1128 168 578 1680 901 262
Cuts at: 6660 820
Size: 1553
Fragments arranged by size:
1680 1553 1128 926 901 578 262 197
168
SnaBI TAC'GTA
Cuts at: 493 5184 493
Size: 4691 2702
SpeI A'CTAG_T
Cuts at: 152 3305 152
Size: 3153 4240
SphI G_CATG'C
Cuts at: 1132 4751 1132
Size: 3619 3774
SspI AAT'ATT
Cuts at: 5 52 2409 2757 4470 5317 5
Size: 47 2357 348 1713 847 2081
Fragments arranged by size:
2357 2081 1713 847 348 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7393
StyI C'CwwG_G
Cuts at: 513 1065 2004 2366 2497 2628 2853 4832 513
Size: 552 939 362 131 131 225 1979 3074
Fragments arranged by size:
3074 1979 939 552 362 225 131 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4276 5420 5437 5590 5775 6220 325
Size: 1255 2696 1144 17 153 185 445 1498
Fragments arranged by size:
2696 1498 1255 1144 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5639 7351 96
Size: 274 80 33 51 1443 3662 1712 138
Fragments arranged by size:
3662 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7353 7385 7353
Size: 32 7361
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7393
XbaI T'CTAG_A
Cuts at: 4729 4729
Size: 7393
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4381 4381
Size: 7393
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7393
XmaI C'CCGG_G
Cuts at: 1870 4718 1870
Size: 2848 4545
XmnI GAAnn'nnTTC
Cuts at: 2925 3435 5522 2925
Size: 510 2087 4796
Fragments arranged by size:
4796 2087 510
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5201 276
Size: 53 83 186 936 3667 2468
Fragments arranged by size:
3667 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI