Restriction map for
pFN21AB3214
(Circular) (Six-base) MAPSORT of: pFN21AB3214.seq Check: 8502 from: 1 to: 7228
pFN21AB3214 7228 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5038 278
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4571 1937
Size: 2634 4594
Acc65I G'GTAC_C
Cuts at: 3508 4549 3508
Size: 1041 6187
AclI AA'CG_TT
Cuts at: 1563 5355 5728 1563
Size: 3792 373 3063
Fragments arranged by size:
3792 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2365 5291 6675 6943 1632
Size: 733 2926 1384 268 1917
Fragments arranged by size:
2926 1917 1384 733 268
AfeI AGC'GCT
Cuts at: 3313 3989 3313
Size: 676 6552
AflII C'TTAA_G
Cuts at: 820 1017 3025 820
Size: 197 2008 5023
Fragments arranged by size:
5023 2008 197
AflIII A'CryG_T
Cuts at: 1203 2673 6127 7104 1203
Size: 1470 3454 977 1327
Fragments arranged by size:
3454 1470 1327 977
AgeI A'CCGG_T
Cuts at: 6059 6059
Size: 7228
AhdI GACnn_n'nnGTC
Cuts at: 3808 5957 3808
Size: 2149 5079
AleI CACnn'nnGTG
Cuts at: 1166 3335 1166
Size: 2169 5059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3111 3353 4284 1184
Size: 1927 242 931 4128
Fragments arranged by size:
4128 1927 931 242
AlwNI CAG_nnn'CTG
Cuts at: 2200 2404 3570 4180 4971 6543 2200
Size: 204 1166 610 791 1572 2885
Fragments arranged by size:
2885 1572 1166 791 610 204
ApaI G_GGCC'C
Cuts at: 2155 2155
Size: 7228
ApaLI G'TGCA_C
Cuts at: 5285 6441 5285
Size: 1156 6072
ApoI r'AATT_y
Cuts at: 1493 4021 4537 4763 4915 7024 1493
Size: 2528 516 226 152 2109 1697
Fragments arranged by size:
2528 2109 1697 516 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5782 160
Size: 872 4750 1606
Fragments arranged by size:
4750 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 4163 4405 4553 1870
Size: 73 684 1536 242 148 4545
Fragments arranged by size:
4545 1536 684 242 148 73
BamHI G'GATC_C
Cuts at: 4558 4925 4558
Size: 367 6861
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 3508 4348 4549 6004
Size: 325 217 627 1601 120 840 201 1455
Cuts at: 6004 618
Size: 1842
Fragments arranged by size:
1842 1601 1455 840 627 325 217 201
120
BanII G_rGCy'C
Cuts at: 1391 2155 2571 2663 3611 4163 4547 1391
Size: 764 416 92 948 552 384 4072
Fragments arranged by size:
4072 948 764 552 416 384 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2462 2496 5419 5453 1903
Size: 34 525 34 2923 34 3678
Fragments arranged by size:
3678 2923 525 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5120 6330 5120
Size: 1210 6018
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7228
BfrBI ATG'CAT
Cuts at: 2738 4098 2738
Size: 1360 5868
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2537 5839
Size: 107 122 71 631 605 487 378 3302
Cuts at: 5839 136
Size: 1525
Fragments arranged by size:
3302 1525 631 605 487 378 122 107
71
BglII A'GATC_T
Cuts at: 3874 7223 3874
Size: 3349 3879
BlpI GC'TnA_GC
Cuts at: 4257 4645 4257
Size: 388 6840
Bme1580I G_kGCm'C
Cuts at: 1790 2155 2643 4305 4351 5289 6445 1790
Size: 365 488 1662 46 938 1156 2573
Fragments arranged by size:
2573 1662 1156 938 488 365 46
BmgBI CAC'GTC
Cuts at: 1318 2119 2638 1318
Size: 801 519 5908
Fragments arranged by size:
5908 801 519
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2838 3069 4959
Size: 416 53 83 399 24 1421 231 1890
Cuts at: 4959 5917 442
Size: 958 1753
Fragments arranged by size:
1890 1753 1421 958 416 399 231 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3658 4446 3658
Size: 788 6440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2907 3011 3292 3754 4213 5888 1239
Size: 1148 520 104 281 462 459 1675 2579
Fragments arranged by size:
2579 1675 1148 520 462 459 281 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 5353 6218 6516 2786
Size: 2567 865 298 3498
Fragments arranged by size:
3498 2567 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3036 3539 5891 882
Size: 617 1537 503 2352 2219
Fragments arranged by size:
2352 2219 1537 617 503
BsaAI yAC'GTr
Cuts at: 493 1204 3193 5019 493
Size: 711 1989 1826 2702
Fragments arranged by size:
2702 1989 1826 711
BsaBI GATnn'nnATC
Cuts at: 7222 7222
Size: 7228
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2901 5035 5417 6052
Size: 53 83 186 936 1368 2134 382 635
Cuts at: 6052 275
Size: 1451
Fragments arranged by size:
2134 1451 1368 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2934 3285 3782 5661 6059 6333 6480 6891 2934
Size: 351 497 1879 398 274 147 411 3271
Fragments arranged by size:
3271 1879 497 411 398 351 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4106 4136 1178
Size: 30 165 30 2703 30 4270
Fragments arranged by size:
4270 2703 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2504 2507 2519 2522 3295 3304 3631
Size: 1072 246 3 12 3 773 9 327
Cuts at: 3631 3634 4399 4422 1186
Size: 3 765 23 3992
Fragments arranged by size:
3992 1072 773 765 327 246 23 12
9 3 3 3
BseYI C'CCAG_C
Cuts at: 2397 2945 2971 2975 3428 3984 4352 6431 6927
Size: 548 26 4 453 556 368 2079 496
Cuts at: 6927 2397
Size: 2698
Fragments arranged by size:
2698 2079 556 548 496 453 368 26
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2047 3842 1163
Size: 573 311 1795 4549
Fragments arranged by size:
4549 1795 573 311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4678 5439 6467 1540
Size: 275 186 2677 761 1028 2301
Fragments arranged by size:
2677 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2663 3332 5289 5374 6445 2663
Size: 669 1957 85 1071 3446
Fragments arranged by size:
3446 1957 1071 669 85
BsmI GAATG_Cn'
Cuts at: 3340 4739 4832 3340
Size: 1399 93 5736
Fragments arranged by size:
5736 1399 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2504 2519 2504
Size: 15 7213
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2571 2643 2663 3332 3611 4163
Size: 399 365 416 72 20 669 279 552
Cuts at: 4163 4305 4351 4547 5289 5374 6445 1391
Size: 142 46 196 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 552 416 399 365
279 196 142 85 72 46 20
BspEI T'CCGG_A
Cuts at: 3782 6891 3782
Size: 3109 4119
BspHI T'CATG_A
Cuts at: 3946 5117 6847 3946
Size: 1171 1730 4327
Fragments arranged by size:
4327 1730 1171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4583 844
Size: 3739 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2915 3603 5115 1394
Size: 429 1092 688 1512 3507
Fragments arranged by size:
3507 1512 1092 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4266 5723 5897 65
Size: 1183 3018 1457 174 1396
Fragments arranged by size:
3018 1457 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3385 3413 5872 6059 1700
Size: 110 1575 28 2459 187 2869
Fragments arranged by size:
2869 2459 1575 187 110 28
BsrGI T'GTAC_A
Cuts at: 96 3717 4493 96
Size: 3621 776 2831
Fragments arranged by size:
3621 2831 776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7228
BssSI C'ACGA_G
Cuts at: 2462 5288 6300 2462
Size: 2826 1012 3390
Fragments arranged by size:
3390 2826 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7228
BstBI TT'CG_AA
Cuts at: 6032 6032
Size: 7228
BstEII G'GTnAC_C
Cuts at: 1184 3140 1184
Size: 1956 5272
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3007 2011
Size: 996 6232
BstYI r'GATC_y
Cuts at: 1972 2996 3874 4170 4558 4925 5310 5327 6779
Size: 1024 878 296 388 367 385 17 1452
Cuts at: 6779 6865 6877 7223 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1024 878 388 385 367 346
296 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2241 2755 3236 2241
Size: 514 481 6233
Fragments arranged by size:
6233 514 481
BtgI C'CryG_G
Cuts at: 513 1065 2964 3222 3448 4209 4221 4461 513
Size: 552 1899 258 226 761 12 240 3280
Fragments arranged by size:
3280 1899 761 552 258 240 226 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3961 487
Size: 683 56 2735 3754
Fragments arranged by size:
3754 2735 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 2185 2289 4293 4744 5538 5558 2059
Size: 126 104 2004 451 794 20 3729
Fragments arranged by size:
3729 2004 794 451 126 104 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7193
DraI TTT'AAA
Cuts at: 4532 4532
Size: 7228
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6235 809
Size: 507 4919 1802
Fragments arranged by size:
4919 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2529 3415
Size: 54 1425 50 275 18 339 360 886
Cuts at: 3415 3462 4675 5564 7193 8
Size: 47 1213 889 1629 43
Fragments arranged by size:
1629 1425 1213 889 886 360 339 275
54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4675 1537
Size: 275 2863 4090
Fragments arranged by size:
4090 2863 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 2844 2856 3580 5158 1353
Size: 1143 348 12 724 1578 3423
Fragments arranged by size:
3423 1578 1143 724 348 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5815 6189 6335 387
Size: 1064 138 4226 374 146 1280
Fragments arranged by size:
4226 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2661 2661
Size: 7228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2365 2387 2907 3011 3292 3754 4213
Size: 393 733 22 520 104 281 462 459
Cuts at: 4213 5291 5888 6675 6943 1239
Size: 1078 597 787 268 1524
Fragments arranged by size:
1524 1078 787 733 597 520 462 459
393 281 268 104 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3025 3168 4133 4381 3025
Size: 143 965 248 5872
Fragments arranged by size:
5872 965 248 143
EcoO109I rG'GnC_Cy
Cuts at: 3242 4177 4427 3242
Size: 935 250 6043
Fragments arranged by size:
6043 935 250
EcoRI G'AATT_C
Cuts at: 4537 4915 4537
Size: 378 6850
FspI TGC'GCA
Cuts at: 2354 4285 5734 2354
Size: 1931 1449 3848
Fragments arranged by size:
3848 1931 1449
HaeII r_GCGC'y
Cuts at: 1678 2201 2458 3315 3991 6375 1678
Size: 523 257 857 676 2384 2531
Fragments arranged by size:
2531 2384 857 676 523 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 3794 3826 4106
Size: 32 135 32 732 32 1458 32 280
Cuts at: 4106 4138 5869 5901 5943 5975 1373
Size: 32 1731 32 42 32 2626
Fragments arranged by size:
2626 1731 1458 732 280 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 4572 4818 669
Size: 988 281 741 1893 246 3079
Fragments arranged by size:
3079 1893 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7228
HpaI GTT'AAC
Cuts at: 2679 4818 2679
Size: 2139 5089
KpnI G_GTAC'C
Cuts at: 3512 4553 3512
Size: 1041 6187
MfeI C'AATT_G
Cuts at: 4827 4827
Size: 7228
MluI A'CGCG_T
Cuts at: 2673 7104 2673
Size: 4431 2797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 3231 3237 3979 4092 6342 6526 1305
Size: 880 1046 6 742 113 2250 184 2007
Fragments arranged by size:
2250 2007 1046 880 742 184 113 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5406
Fragments arranged by size:
5406 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2039 3335 5186 5545 5704 518
Size: 648 843 30 1296 1851 359 159 2042
Fragments arranged by size:
2042 1851 1296 843 648 359 159 30
MspA1I CmG'CkG
Cuts at: 1606 2095 2272 2337 2975 3501 4644 5321 6469
Size: 489 177 65 638 526 1143 677 1148
Cuts at: 6469 6714 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1143 677 638 526 489 245
177 65
NaeI GCC'GGC
Cuts at: 3415 3415
Size: 7228
NcoI C'CATG_G
Cuts at: 513 1065 3222 4209 513
Size: 552 2157 987 3532
Fragments arranged by size:
3532 2157 987 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7228
NgoMIV G'CCGG_C
Cuts at: 3413 3413
Size: 7228
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7228
NotI GC'GGCC_GC
Cuts at: 4675 4675
Size: 7228
NsiI A_TGCA'T
Cuts at: 2740 4100 2740
Size: 1360 5868
NspI r_CATG'y
Cuts at: 1132 1229 2178 2359 2718 4098 4586 6131 1132
Size: 97 949 181 359 1380 488 1545 2229
Fragments arranged by size:
2229 1545 1380 949 488 359 181 97
PasI CC'CwG_GG
Cuts at: 1348 2547 3658 4344 1348
Size: 1199 1111 686 4232
Fragments arranged by size:
4232 1199 1111 686
PciI A'CATG_T
Cuts at: 6127 6127
Size: 7228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7228
PfoI T'CCnGG_A
Cuts at: 2775 2999 2775
Size: 224 7004
PmeI GTTT'AAAC
Cuts at: 4532 4532
Size: 7228
PmlI CAC'GTG
Cuts at: 3193 3193
Size: 7228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5297 5329 6810 6842 5297
Size: 32 1481 32 5683
Fragments arranged by size:
5683 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4427 4427
Size: 7228
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7228
PsiI TTA'TAA
Cuts at: 4798 4798
Size: 7228
PspOMI G'GGCC_C
Cuts at: 2151 2151
Size: 7228
PstI C_TGCA'G
Cuts at: 830 2394 2485 3620 4580 830
Size: 1564 91 1135 960 3478
Fragments arranged by size:
3478 1564 1135 960 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7228
PvuII CAG'CTG
Cuts at: 2975 3501 2975
Size: 526 6702
SacI G_AGCT'C
Cuts at: 2663 2663
Size: 7228
SalI G'TCGA_C
Cuts at: 1936 4570 1936
Size: 2634 4594
SapI GCTCTTCn'nnn_
Cuts at: 3580 3580
Size: 7228
SbfI CC_TGCA'GG
Cuts at: 4580 4580
Size: 7228
ScaI AGT'ACT
Cuts at: 5476 7188 5476
Size: 1712 5516
SfcI C'TryA_G
Cuts at: 826 1045 2390 2481 3616 3858 4576 5711 6392
Size: 219 1345 91 1135 242 718 1135 681
Cuts at: 6392 6583 826
Size: 191 1471
Fragments arranged by size:
1471 1345 1135 1135 718 681 242 219
191 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7228
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7228
SmaI CCC'GGG
Cuts at: 1872 4407 4555 1872
Size: 2535 148 4545
Fragments arranged by size:
4545 2535 148
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2801 3025 5332 6233 6495 820
Size: 197 926 858 224 2307 901 262 1553
Fragments arranged by size:
2307 1553 926 901 858 262 224 197
SnaBI TAC'GTA
Cuts at: 493 5019 493
Size: 4526 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7228
SphI G_CATG'C
Cuts at: 1132 2178 2359 4098 4586 1132
Size: 1046 181 1739 488 3774
Fragments arranged by size:
3774 1739 1046 488 181
SspI AAT'ATT
Cuts at: 5 52 2186 5152 5
Size: 47 2134 2966 2081
Fragments arranged by size:
2966 2134 2081 47
StuI AGG'CCT
Cuts at: 1517 3440 3699 1517
Size: 1923 259 5046
Fragments arranged by size:
5046 1923 259
StyI C'CwwG_G
Cuts at: 513 1065 2652 2904 3222 3540 3881 4209 4248
Size: 552 1587 252 318 318 341 328 39
Cuts at: 4248 4667 513
Size: 419 3074
Fragments arranged by size:
3074 1587 552 419 341 328 318 318
252 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3394 4241 4366 5255 5272 5425 5610
Size: 1255 1814 847 125 889 17 153 185
Cuts at: 5610 6055 325
Size: 445 1498
Fragments arranged by size:
1814 1498 1255 889 847 445 185 153
125 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3077 3717 4493
Size: 274 80 33 51 1443 1100 640 776
Cuts at: 4493 5474 7186 96
Size: 981 1712 138
Fragments arranged by size:
1712 1443 1100 981 776 640 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2432 2464 3862 3894 7188 7220 2432
Size: 32 1398 32 3294 32 2440
Fragments arranged by size:
3294 2440 1398 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7228
XbaI T'CTAG_A
Cuts at: 4564 4564
Size: 7228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 3220 3399 2074
Size: 1146 179 5903
Fragments arranged by size:
5903 1146 179
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7228
XmaI C'CCGG_G
Cuts at: 1870 4405 4553 1870
Size: 2535 148 4545
Fragments arranged by size:
4545 2535 148
XmnI GAAnn'nnTTC
Cuts at: 2144 3890 5357 2144
Size: 1746 1467 4015
Fragments arranged by size:
4015 1746 1467
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5036 276
Size: 53 83 186 936 3502 2468
Fragments arranged by size:
3502 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI PacI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI