Restriction map for
pFN21AB3201
(Circular) (Six-base) MAPSORT of: pFN21AB3201.seq Check: 1742 from: 1 to: 5743
pFN21AB3201 5743 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2770 3806 5190 5458 1632
Size: 1138 1036 1384 268 1917
Fragments arranged by size:
1917 1384 1138 1036 268
AflII C'TTAA_G
Cuts at: 820 1017 2182 820
Size: 197 1165 4381
Fragments arranged by size:
4381 1165 197
AflIII A'CryG_T
Cuts at: 1203 2355 2589 2721 4642 5619 1203
Size: 1152 234 132 1921 977 1327
Fragments arranged by size:
1921 1327 1152 977 234 132
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 2020 4472 2020
Size: 2452 3291
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2872 3486 5058 2872
Size: 614 1572 3557
Fragments arranged by size:
3557 1572 614
ApaI G_GGCC'C
Cuts at: 2545 2545
Size: 5743
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2546 3068 1870
Size: 73 82 521 522 4545
Fragments arranged by size:
4545 522 521 82 73
BamHI G'GATC_C
Cuts at: 2400 3073 3440 2400
Size: 673 367 4703
Fragments arranged by size:
4703 673 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2498 2545 3062 1391
Size: 1107 47 517 4072
Fragments arranged by size:
4072 1107 517 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2723 928
Size: 971 824 3948
Fragments arranged by size:
3948 971 824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 2662 1548
Size: 1114 4629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2867 4354 136
Size: 107 122 71 631 605 1195 1487 1525
Fragments arranged by size:
1525 1487 1195 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2812 5738 2812
Size: 2926 2817
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2545 2696 3804 4960 1790
Size: 755 151 1108 1156 2573
Fragments arranged by size:
2573 1156 1108 755 151
BmgBI CAC'GTC
Cuts at: 1318 2017 1318
Size: 699 5044
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4403 1239
Size: 3164 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 4406 882
Size: 617 738 2169 2219
Fragments arranged by size:
2219 2169 738 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 1178
Size: 30 165 30 651 30 4837
Fragments arranged by size:
4837 651 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5743
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2811 1163
Size: 573 1075 4095
Fragments arranged by size:
4095 1075 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2851 3804 3889 4960 2851
Size: 953 85 1071 3634
Fragments arranged by size:
3634 1071 953 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
BsmBI CGTCTCn'nnnn_
Cuts at: 2793 2793
Size: 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2498 2545 2696 2851 3062 3804 3889
Size: 399 708 47 151 155 211 742 85
Cuts at: 3889 4960 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 708 399 211 155 151
85 47
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2400 2413 2812 3073 3440 3825 3842 5294
Size: 428 13 399 261 367 385 17 1452
Cuts at: 5294 5380 5392 5738 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 428 399 385 367 346 261
86 17 13 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2937 4750 809
Size: 507 1621 1813 1802
Fragments arranged by size:
1813 1802 1621 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2478 2714 2859
Size: 54 1425 50 275 18 648 236 145
Cuts at: 2859 2897 3190 4079 5708 8
Size: 38 293 889 1629 43
Fragments arranged by size:
1629 1425 889 648 293 275 236 145
54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2373 3673 1353
Size: 1020 1300 3423
Fragments arranged by size:
3423 1300 1020
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2770 3806 4403 5190 5458 1239
Size: 393 1138 1036 597 787 268 1524
Fragments arranged by size:
1524 1138 1036 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 5743
EcoO109I rG'GnC_Cy
Cuts at: 2542 2542
Size: 5743
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2652 2068
Size: 584 5159
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 4384 4416 4458
Size: 32 135 32 434 32 2346 32 42
Cuts at: 4458 4490 1373
Size: 32 2626
Fragments arranged by size:
2626 2346 434 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2480 10
Size: 54 1425 343 648 3273
Fragments arranged by size:
3273 1425 648 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2914 3701 4060 4219 518
Size: 648 1194 554 787 359 159 2042
Fragments arranged by size:
2042 1194 787 648 554 359 159
MspA1I CmG'CkG
Cuts at: 1606 2923 3159 3836 4984 5229 1606
Size: 1317 236 677 1148 245 2120
Fragments arranged by size:
2120 1317 1148 677 245 236
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2359 2593 2725 3101 4646 1132
Size: 97 1130 234 132 376 1545 2229
Fragments arranged by size:
2229 1545 1130 376 234 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 2355 2589 2721 4642 2355
Size: 234 132 1921 3456
Fragments arranged by size:
3456 1921 234 132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2912 1108
Size: 1804 3939
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 2306 3313 2306
Size: 1007 4736
PspOMI G'GGCC_C
Cuts at: 2541 2541
Size: 5743
PstI C_TGCA'G
Cuts at: 830 3095 830
Size: 2265 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2923 2923
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SexAI A'CCwGG_T
Cuts at: 2528 2528
Size: 5743
SfcI C'TryA_G
Cuts at: 826 1045 3091 4226 4907 5098 826
Size: 219 2046 1135 681 191 1471
Fragments arranged by size:
2046 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2182 3847 4748 5010 820
Size: 197 926 239 1665 901 262 1553
Fragments arranged by size:
1665 1553 926 901 262 239 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 2263 2384 2679 3667 5
Size: 47 2211 121 295 988 2081
Fragments arranged by size:
2211 2081 988 295 121 47
StuI AGG'CCT
Cuts at: 1517 3005 1517
Size: 1488 4255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 3182 513
Size: 552 939 52 1126 3074
Fragments arranged by size:
3074 1126 939 552 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2560 2592 5703 5735 2560
Size: 32 3111 32 2568
Fragments arranged by size:
3111 2568 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 2296 3079 2296
Size: 783 4960
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 2885 3872 2885
Size: 987 4756
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI