Restriction map for
pFN21AB3191
(Circular) (Six-base) MAPSORT of: pFN21AB3191.seq Check: 7547 from: 1 to: 6472
pFN21AB3191 6472 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3729 4282 278
Size: 53 83 186 936 2193 553 2468
Fragments arranged by size:
2468 2193 936 553 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2781 3143 3422 4535 5919 6187 1632
Size: 1149 362 279 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1149 1113 362 279 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 3022 5371 6348 1203
Size: 1819 2349 977 1327
Fragments arranged by size:
2349 1819 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3539 1184
Size: 2355 4117
AlwNI CAG_nnn'CTG
Cuts at: 2707 2872 3214 4215 5787 2707
Size: 165 342 1001 1572 3392
Fragments arranged by size:
3392 1572 1001 342 165
ApaI G_GGCC'C
Cuts at: 3439 3651 3439
Size: 212 6260
ApaLI G'TGCA_C
Cuts at: 2261 4529 5685 2261
Size: 2268 1156 3048
Fragments arranged by size:
3048 2268 1156
ApoI r'AATT_y
Cuts at: 1493 3781 4007 4159 6268 1493
Size: 2288 226 152 2109 1697
Fragments arranged by size:
2288 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2472 3797 1870
Size: 73 529 1325 4545
Fragments arranged by size:
4545 1325 529 73
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3793 5248 618
Size: 325 217 627 2006 1455 1842
Fragments arranged by size:
2006 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2814 3439 3651 3791 1391
Size: 1423 625 212 140 4072
Fragments arranged by size:
4072 1423 625 212 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2769 928
Size: 971 870 4631
Fragments arranged by size:
4631 971 870
BbvCI CC'TCA_GC
Cuts at: 3240 3240
Size: 6472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 2967 1548
Size: 1419 5053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3746 5083
Size: 107 122 71 631 605 337 1737 1337
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
1737 1525 1337 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2979 6467 2979
Size: 3488 2984
BlpI GC'TnA_GC
Cuts at: 3117 3889 3117
Size: 772 5700
Bme1580I G_kGCm'C
Cuts at: 1790 2265 3137 3439 3651 4533 5689 1790
Size: 475 872 302 212 882 1156 2573
Fragments arranged by size:
2573 1156 882 872 475 302 212
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3202 4203 5161
Size: 416 53 83 399 24 1785 1001 958
Cuts at: 5161 442
Size: 1753
Fragments arranged by size:
1785 1753 1001 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2396 2612 2744 2798 5132 1239
Size: 1064 93 216 132 54 2334 2579
Fragments arranged by size:
2579 2334 1064 216 132 93 54
Bpu10I CC'TnA_GC
Cuts at: 3240 3240
Size: 6472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4597 5462 5760 4597
Size: 865 298 5309
Fragments arranged by size:
5309 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3062 3471 5135 882
Size: 617 1563 409 1664 2219
Fragments arranged by size:
2219 1664 1563 617 409
BsaAI yAC'GTr
Cuts at: 493 1204 2260 4263 493
Size: 711 1056 2003 2702
Fragments arranged by size:
2702 2003 1056 711
BsaBI GATnn'nnATC
Cuts at: 3191 6466 3191
Size: 3275 3197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3246 3726 4279 4661
Size: 53 83 186 936 1713 480 553 382
Cuts at: 4661 5296 275
Size: 635 1451
Fragments arranged by size:
1713 1451 936 635 553 480 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2514 3473 4905 5303 5577 5724 6135 2514
Size: 959 1432 398 274 147 411 2851
Fragments arranged by size:
2851 1432 959 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2990 3020 1178
Size: 30 165 30 1587 30 4630
Fragments arranged by size:
4630 1587 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 1186
Size: 1132 5340
BseYI C'CCAG_C
Cuts at: 2221 2569 2700 3304 3590 5675 6171 2221
Size: 348 131 604 286 2085 496 2522
Fragments arranged by size:
2522 2085 604 496 348 286 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3476 1163
Size: 573 1740 4159
Fragments arranged by size:
4159 1740 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3922 4683 5711 1540
Size: 275 186 15 1906 761 1028 2301
Fragments arranged by size:
2301 1906 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2052 2265 2361 2528 2814 3096 4533 4618 5689
Size: 213 96 167 286 282 1437 85 1071
Cuts at: 5689 2052
Size: 2835
Fragments arranged by size:
2835 1437 1071 286 282 213 167 96
85
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2265 2361 2528 2814 3096 3137
Size: 399 262 213 96 167 286 282 41
Cuts at: 3137 3439 3651 3791 4533 4618 5689 1391
Size: 302 212 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 302 286 282 262
213 212 167 140 96 85 41
BspEI T'CCGG_A
Cuts at: 2514 3473 6135 2514
Size: 959 2662 2851
Fragments arranged by size:
2851 2662 959
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3498 3827 844
Size: 2654 329 3489
Fragments arranged by size:
3489 2654 329
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2074 4967 5141 65
Size: 1183 826 2893 174 1396
Fragments arranged by size:
2893 1396 1183 826 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5116 5303 1700
Size: 110 3306 187 2869
Fragments arranged by size:
3306 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 2354 4532 5544 2354
Size: 2178 1012 3282
Fragments arranged by size:
3282 2178 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2269 2389 3100 794
Size: 1475 120 711 4166
Fragments arranged by size:
4166 1475 711 120
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2590 3463 2122
Size: 468 873 5131
Fragments arranged by size:
5131 873 468
BstYI r'GATC_y
Cuts at: 1972 2719 2979 3802 4169 4554 4571 6023 6109
Size: 747 260 823 367 385 17 1452 86
Cuts at: 6109 6121 6467 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 823 747 385 367 346 260
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3138 3138
Size: 6472
BtgI C'CryG_G
Cuts at: 513 1065 2004 2541 3573 513
Size: 552 939 537 1032 3412
Fragments arranged by size:
3412 1032 939 552 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2026 2101 2680 487
Size: 683 56 800 75 579 4279
Fragments arranged by size:
4279 800 683 579 75 56
BtsI GCAGTG_nn'
Cuts at: 2263 2334 2829 3988 4782 4802 2263
Size: 71 495 1159 794 20 3933
Fragments arranged by size:
3933 1159 794 495 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DraIII CAC_nnn'GTG
Cuts at: 2389 2389
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3760 5479 809
Size: 507 2444 1719 1802
Fragments arranged by size:
2444 1802 1719 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2292 3267
Size: 54 1425 50 275 18 183 279 975
Cuts at: 3267 3465 3624 3919 4808 6437 8
Size: 198 159 295 889 1629 43
Fragments arranged by size:
1629 1425 975 889 295 279 275 198
183 159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 3919 1537
Size: 275 201 1906 4090
Fragments arranged by size:
4090 1906 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 3113 3515 4402 1353
Size: 1106 654 402 887 3423
Fragments arranged by size:
3423 1106 887 654 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 5059 5433 5579 387
Size: 1064 138 921 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 921 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 2812
Size: 6472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2396 2612 2744 2781 2798 3143
Size: 393 671 93 216 132 37 17 345
Cuts at: 3143 3422 4535 5132 5919 6187 1239
Size: 279 1113 597 787 268 1524
Fragments arranged by size:
1524 1113 787 671 597 393 345 279
268 216 132 93 37 17
EcoO109I rG'GnC_Cy
Cuts at: 2142 2197 2728 2828 3595 3647 2142
Size: 55 531 100 767 52 4967
Fragments arranged by size:
4967 767 531 100 55 52
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FspI TGC'GCA
Cuts at: 3584 4978 3584
Size: 1394 5078
HaeII r_GCGC'y
Cuts at: 1678 5619 1678
Size: 3941 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2728 2760 2990 3022 5113
Size: 32 135 32 1156 32 230 32 2091
Cuts at: 5113 5145 5187 5219 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2091 1156 230 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3816 4062 669
Size: 988 281 1878 246 3079
Fragments arranged by size:
3079 1878 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2658 5586 5770 1305
Size: 1353 2928 184 2007
Fragments arranged by size:
2928 2007 1353 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 3269 3467 3626 10
Size: 54 1425 343 462 975 198 159 2856
Fragments arranged by size:
2856 1425 975 462 343 198 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2540 2624 3369 3521 4430 4789
Size: 648 879 495 84 745 152 909 359
Cuts at: 4789 4948 518
Size: 159 2042
Fragments arranged by size:
2042 909 879 745 648 495 359 159
152 84
MspA1I CmG'CkG
Cuts at: 1606 2221 2281 2759 2948 3121 3202 3304 3888
Size: 615 60 478 189 173 81 102 584
Cuts at: 3888 4565 5713 5958 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 615 584 478 245 189
173 102 81 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 2541 513
Size: 552 939 537 4444
Fragments arranged by size:
4444 939 552 537
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 3026 3286 3568 3830 5375 1132
Size: 97 1797 260 282 262 1545 2229
Fragments arranged by size:
2229 1797 1545 282 262 260 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6472
PciI A'CATG_T
Cuts at: 3022 5371 3022
Size: 2349 4123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3721 1108
Size: 2613 3859
PfoI T'CCnGG_A
Cuts at: 3654 3654
Size: 6472
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2542 3143 3175 4541 4573 6054 6086 2510
Size: 32 601 32 1366 32 1481 32 2896
Fragments arranged by size:
2896 1481 1366 601 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2142 2197 3595 2142
Size: 55 1398 5019
Fragments arranged by size:
5019 1398 55
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PspOMI G'GGCC_C
Cuts at: 3435 3647 3435
Size: 212 6260
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2218 2250 2218
Size: 32 6440
PstI C_TGCA'G
Cuts at: 830 3639 3824 830
Size: 2809 185 3478
Fragments arranged by size:
3478 2809 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2221 2948 3121 3202 3304 2221
Size: 727 173 81 102 5389
Fragments arranged by size:
5389 727 173 102 81
SacI G_AGCT'C
Cuts at: 2814 2814
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SanDI GG'GwC_CC
Cuts at: 3595 3595
Size: 6472
SapI GCTCTTCn'nnn_
Cuts at: 3515 3515
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 2510 4720 6432 2510
Size: 2210 1712 2550
Fragments arranged by size:
2550 2210 1712
SexAI A'CCwGG_T
Cuts at: 2850 2850
Size: 6472
SfcI C'TryA_G
Cuts at: 826 1045 3635 3820 4955 5636 5827 826
Size: 219 2590 185 1135 681 191 1471
Fragments arranged by size:
2590 1471 1135 681 219 191 185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3799 1872
Size: 1927 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4576 5477 5739 820
Size: 197 926 2633 901 262 1553
Fragments arranged by size:
2633 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2128 2541 2583 3456 3911
Size: 552 939 117 7 413 42 873 455
Cuts at: 3911 513
Size: 3074
Fragments arranged by size:
3074 939 873 552 455 413 117 42
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4499 4516 4669 4854 5299 325
Size: 1255 2919 17 153 185 445 1498
Fragments arranged by size:
2919 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 3279 4718
Size: 274 80 33 51 1443 531 771 1439
Cuts at: 4718 6430 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1439 771 531 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 3236 3268 6432 6464 2134
Size: 32 1070 32 3164 32 2142
Fragments arranged by size:
3164 2142 1070 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2603 2852 1316
Size: 1287 249 4936
Fragments arranged by size:
4936 1287 249
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2848 3232 2119
Size: 729 384 5359
Fragments arranged by size:
5359 729 384
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 3797 1870
Size: 1927 4545
XmnI GAAnn'nnTTC
Cuts at: 4601 4601
Size: 6472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3727 4280 276
Size: 53 83 186 936 2193 553 2468
Fragments arranged by size:
2468 2193 936 553 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacII SfiI SfoI SrfI SwaI