Restriction map for
pFN21AB3168
(Circular) (Six-base) MAPSORT of: pFN21AB3168.seq Check: 204 from: 1 to: 7075
pFN21AB3168 7075 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4885 278
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4418 1937
Size: 2481 4594
Acc65I G'GTAC_C
Cuts at: 4396 4396
Size: 7075
AclI AA'CG_TT
Cuts at: 1563 3493 5202 5575 1563
Size: 1930 1709 373 3063
Fragments arranged by size:
3063 1930 1709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 2813 5138 6522 6790 1632
Size: 914 267 2325 1384 268 1917
Fragments arranged by size:
2325 1917 1384 914 268 267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6878
AflIII A'CryG_T
Cuts at: 1203 2337 5974 6951 1203
Size: 1134 3637 977 1327
Fragments arranged by size:
3637 1327 1134 977
AgeI A'CCGG_T
Cuts at: 5906 5906
Size: 7075
AhdI GACnn_n'nnGTC
Cuts at: 5804 5804
Size: 7075
AleI CACnn'nnGTG
Cuts at: 1166 4251 1166
Size: 3085 3990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4130 1184
Size: 2946 4129
AlwNI CAG_nnn'CTG
Cuts at: 2885 4818 6390 2885
Size: 1933 1572 3570
Fragments arranged by size:
3570 1933 1572
ApaI G_GGCC'C
Cuts at: 3284 4238 4246 4297 3284
Size: 954 8 51 6062
Fragments arranged by size:
6062 954 51 8
ApaLI G'TGCA_C
Cuts at: 5132 6288 5132
Size: 1156 5919
ApoI r'AATT_y
Cuts at: 1493 2420 2456 2697 4384 4610 4762 6871 1493
Size: 927 36 241 1687 226 152 2109 1697
Fragments arranged by size:
2109 1697 1687 927 241 226 152 36
AseI AT'TA_AT
Cuts at: 160 1032 5629 160
Size: 872 4597 1606
Fragments arranged by size:
4597 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 3318 3557 4155 4400 1870
Size: 73 1365 10 239 598 245 4545
Fragments arranged by size:
4545 1365 598 245 239 73 10
BamHI G'GATC_C
Cuts at: 3553 4405 4772 3553
Size: 852 367 5856
Fragments arranged by size:
5856 852 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3992 4396 5851 618
Size: 325 217 627 2205 404 1455 1842
Fragments arranged by size:
2205 1842 1455 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 3284 3294 3339 3446 3725 4200 4238 4246
Size: 1893 10 45 107 279 475 38 8
Cuts at: 4246 4297 4394 1391
Size: 51 97 4072
Fragments arranged by size:
4072 1893 475 279 107 97 51 45
38 10 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 2862 4275 928
Size: 971 192 771 1413 3728
Fragments arranged by size:
3728 1413 971 771 192
BbvCI CC'TCA_GC
Cuts at: 3656 3656
Size: 7075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3289 3323 3746 3780 5266 5300 1903
Size: 34 1352 34 423 34 1486 34 3678
Fragments arranged by size:
3678 1486 1352 423 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 4967 6177 4013
Size: 954 1210 4911
Fragments arranged by size:
4911 1210 954
BclI T'GATC_A
Cuts at: 1548 4306 1548
Size: 2758 4317
BfrBI ATG'CAT
Cuts at: 2320 2320
Size: 7075
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5686 136
Size: 107 122 71 631 605 4014 1525
Fragments arranged by size:
4014 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7070 7070
Size: 7075
BlpI GC'TnA_GC
Cuts at: 4492 4492
Size: 7075
Bme1580I G_kGCm'C
Cuts at: 1790 3108 3138 3284 4238 4246 4297 5136 6292
Size: 1318 30 146 954 8 51 839 1156
Cuts at: 6292 1790
Size: 2573
Fragments arranged by size:
2573 1318 1156 954 839 146 51 30
8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4806 5764
Size: 416 53 83 399 24 1958 1431 958
Cuts at: 5764 442
Size: 1753
Fragments arranged by size:
1958 1753 1431 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7075
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 7075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2456 3563 3634 3840 5735 1239
Size: 825 392 1107 71 206 1895 2579
Fragments arranged by size:
2579 1895 1107 825 392 206 71
Bpu10I CC'TnA_GC
Cuts at: 3656 3656
Size: 7075
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 3765 4123 5200 6065 6363 2378
Size: 1387 358 1077 865 298 3090
Fragments arranged by size:
3090 1387 1077 865 358 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2832 3413 5738 882
Size: 617 1333 581 2325 2219
Fragments arranged by size:
2325 2219 1333 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 4866 493
Size: 711 3662 2702
Fragments arranged by size:
3662 2702 711
BsaBI GATnn'nnATC
Cuts at: 2936 7069 2936
Size: 4133 2942
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3759 4882 5264 5899
Size: 53 83 186 936 2226 1123 382 635
Cuts at: 5899 275
Size: 1451
Fragments arranged by size:
2226 1451 1123 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2271 3717 5508 5906 6180 6327 6738 2271
Size: 1446 1791 398 274 147 411 2608
Fragments arranged by size:
2608 1791 1446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6850
Fragments arranged by size:
6850 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3109 3371 3637 4208 1186
Size: 1923 262 266 571 4053
Fragments arranged by size:
4053 1923 571 266 262
BseYI C'CCAG_C
Cuts at: 2952 3138 3204 3398 6278 6774 2952
Size: 186 66 194 2880 496 3253
Fragments arranged by size:
3253 2880 496 194 186 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3457 3491 3771 3844 1163
Size: 573 1721 34 280 73 4394
Fragments arranged by size:
4394 1721 573 280 73 34
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4525 5286 6314 1540
Size: 275 186 2524 761 1028 2301
Fragments arranged by size:
2524 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3227 4200 5136 5221 6292 3227
Size: 973 936 85 1071 4010
Fragments arranged by size:
4010 1071 973 936 85
BsmI GAATG_Cn'
Cuts at: 2735 3733 3992 4352 4586 4679 2735
Size: 998 259 360 234 93 5131
Fragments arranged by size:
5131 998 360 259 234 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3108 3138 3227 3284 3294 3339 3446
Size: 399 1318 30 89 57 10 45 107
Cuts at: 3446 3725 4200 4238 4246 4297 4394 5136 5221
Size: 279 475 38 8 51 97 742 85
Cuts at: 5221 6292 1391
Size: 1071 2174
Fragments arranged by size:
2174 1318 1071 742 475 399 279 107
97 89 85 57 51 45 38 30
10 8
BspEI T'CCGG_A
Cuts at: 2271 3717 6738 2271
Size: 1446 3021 2608
Fragments arranged by size:
3021 2608 1446
BspHI T'CATG_A
Cuts at: 4063 4964 6694 4063
Size: 901 1730 4444
Fragments arranged by size:
4444 1730 901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4430 844
Size: 3586 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4962 1394
Size: 429 3139 3507
Fragments arranged by size:
3507 3139 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4324 5570 5744 65
Size: 1183 3076 1246 174 1396
Fragments arranged by size:
3076 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3833 5719 5906 1700
Size: 110 2023 1886 187 2869
Fragments arranged by size:
2869 2023 1886 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7075
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7075
BssSI C'ACGA_G
Cuts at: 5135 6147 5135
Size: 1012 6063
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3618 4347 794
Size: 2824 729 3522
Fragments arranged by size:
3522 2824 729
BstBI TT'CG_AA
Cuts at: 5879 5879
Size: 7075
BstEII G'GTnAC_C
Cuts at: 1184 2785 1184
Size: 1601 5474
BstXI CCAn_nnnn'nTGG
Cuts at: 3749 3749
Size: 7075
BstYI r'GATC_y
Cuts at: 1972 2881 3553 3606 3700 4405 4772 5157 5174
Size: 909 672 53 94 705 367 385 17
Cuts at: 5174 6626 6712 6724 7070 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 909 705 672 385 367 346
94 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2025 3285 3354 3925 2025
Size: 1260 69 571 5175
Fragments arranged by size:
5175 1260 571 69
BtgI C'CryG_G
Cuts at: 513 1065 2004 2544 2959 3120 513
Size: 552 939 540 415 161 4468
Fragments arranged by size:
4468 939 552 540 415 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6336
Fragments arranged by size:
6336 683 56
BtsI GCAGTG_nn'
Cuts at: 3423 4591 5385 5405 3423
Size: 1168 794 20 5093
Fragments arranged by size:
5093 1168 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7040
DraI TTT'AAA
Cuts at: 2095 2125 2419 4379 2095
Size: 30 294 1960 4791
Fragments arranged by size:
4791 1960 294 30
DraIII CAC_nnn'GTG
Cuts at: 3882 3882
Size: 7075
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6082 809
Size: 507 4766 1802
Fragments arranged by size:
4766 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4522 5411 7040
Size: 54 1425 50 275 18 2692 889 1629
Cuts at: 7040 8
Size: 43
Fragments arranged by size:
2692 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4522 1537
Size: 275 2710 4090
Fragments arranged by size:
4090 2710 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4077 5005 1353
Size: 2724 928 3423
Fragments arranged by size:
3423 2724 928
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5662 6036 6182 387
Size: 1064 138 4073 374 146 1280
Fragments arranged by size:
4073 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4198 4198
Size: 7075
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2456 2546 2813 3563 3634 3840
Size: 393 432 392 90 267 750 71 206
Cuts at: 3840 5138 5735 6522 6790 1239
Size: 1298 597 787 268 1524
Fragments arranged by size:
1524 1298 787 750 597 432 393 392
268 267 206 90 71
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2024
Size: 7075
EcoO109I rG'GnC_Cy
Cuts at: 3281 3312 3371 4235 4242 4294 3281
Size: 31 59 864 7 52 6062
Fragments arranged by size:
6062 864 59 52 31 7
EcoRI G'AATT_C
Cuts at: 4384 4762 4384
Size: 378 6697
FspI TGC'GCA
Cuts at: 5581 5581
Size: 7075
HaeII r_GCGC'y
Cuts at: 1678 2288 2381 3308 6222 1678
Size: 610 93 927 2914 2531
Fragments arranged by size:
2914 2531 927 610 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 5716 5748 5790
Size: 32 135 32 529 32 3583 32 42
Cuts at: 5790 5822 1373
Size: 32 2626
Fragments arranged by size:
3583 2626 529 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2833 4419 4665 669
Size: 988 281 895 1586 246 3079
Fragments arranged by size:
3079 1586 988 895 281 246
HindIII A'AGCT_T
Cuts at: 748 2276 748
Size: 1528 5547
HpaI GTT'AAC
Cuts at: 2833 4665 2833
Size: 1832 5243
KpnI G_GTAC'C
Cuts at: 4400 4400
Size: 7075
MfeI C'AATT_G
Cuts at: 2308 4674 2308
Size: 2366 4709
MluI A'CGCG_T
Cuts at: 6951 6951
Size: 7075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2973 6189 6373 1305
Size: 1668 3216 184 2007
Fragments arranged by size:
3216 2007 1668 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5253
Fragments arranged by size:
5253 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 3194 3428 4251 5033 5392 5551
Size: 648 1377 651 234 823 782 359 159
Cuts at: 5551 518
Size: 2042
Fragments arranged by size:
2042 1377 823 782 651 648 359 234
159
MspA1I CmG'CkG
Cuts at: 1606 2427 4491 5168 6316 6561 1606
Size: 821 2064 677 1148 245 2120
Fragments arranged by size:
2120 2064 1148 821 677 245
NaeI GCC'GGC
Cuts at: 3835 3835
Size: 7075
NcoI C'CATG_G
Cuts at: 513 1065 2004 2544 2959 3120 513
Size: 552 939 540 415 161 4468
Fragments arranged by size:
4468 939 552 540 415 161
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7075
NgoMIV G'CCGG_C
Cuts at: 3833 3833
Size: 7075
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7075
NotI GC'GGCC_GC
Cuts at: 4522 4522
Size: 7075
NsiI A_TGCA'T
Cuts at: 2322 2322
Size: 7075
NspI r_CATG'y
Cuts at: 1132 1229 2324 2341 4433 5978 1132
Size: 97 1095 17 2092 1545 2229
Fragments arranged by size:
2229 2092 1545 1095 97 17
PasI CC'CwG_GG
Cuts at: 1348 4238 1348
Size: 2890 4185
PciI A'CATG_T
Cuts at: 2337 5974 2337
Size: 3637 3438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4231 1108
Size: 3123 3952
PfoI T'CCnGG_A
Cuts at: 3579 3579
Size: 7075
PmeI GTTT'AAAC
Cuts at: 4379 4379
Size: 7075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5144 5176 6657 6689 5144
Size: 32 1481 32 5530
Fragments arranged by size:
5530 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7075
PsiI TTA'TAA
Cuts at: 4645 4645
Size: 7075
PspOMI G'GGCC_C
Cuts at: 3280 4234 4242 4293 3280
Size: 954 8 51 6062
Fragments arranged by size:
6062 954 51 8
PstI C_TGCA'G
Cuts at: 830 3614 4427 830
Size: 2784 813 3478
Fragments arranged by size:
3478 2784 813
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7075
SacI G_AGCT'C
Cuts at: 4200 4200
Size: 7075
SalI G'TCGA_C
Cuts at: 1936 4417 1936
Size: 2481 4594
SapI GCTCTTCn'nnn_
Cuts at: 4077 4077
Size: 7075
SbfI CC_TGCA'GG
Cuts at: 4427 4427
Size: 7075
ScaI AGT'ACT
Cuts at: 2921 3616 5323 7035 2921
Size: 695 1707 1712 2961
Fragments arranged by size:
2961 1712 1707 695
SfcI C'TryA_G
Cuts at: 826 1045 2734 3610 3633 4423 5558 6239 6430
Size: 219 1689 876 23 790 1135 681 191
Cuts at: 6430 826
Size: 1471
Fragments arranged by size:
1689 1471 1135 876 790 681 219 191
23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7075
SmaI CCC'GGG
Cuts at: 1872 3559 4157 4402 1872
Size: 1687 598 245 4545
Fragments arranged by size:
4545 1687 598 245
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3780 4138 5179 6080 6342
Size: 197 926 450 1387 358 1041 901 262
Cuts at: 6342 820
Size: 1553
Fragments arranged by size:
1553 1387 1041 926 901 450 358 262
197
SnaBI TAC'GTA
Cuts at: 493 4866 493
Size: 4373 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7075
SphI G_CATG'C
Cuts at: 1132 2324 4433 1132
Size: 1192 2109 3774
Fragments arranged by size:
3774 2109 1192
SrfI GCCC'GGGC
Cuts at: 4157 4157
Size: 7075
SspI AAT'ATT
Cuts at: 5 52 4999 5
Size: 47 4947 2081
Fragments arranged by size:
4947 2081 47
StuI AGG'CCT
Cuts at: 1517 3644 1517
Size: 2127 4948
StyI C'CwwG_G
Cuts at: 513 1065 2004 2544 2781 2947 2959 3120 3339
Size: 552 939 540 237 166 12 161 219
Cuts at: 3339 3997 4514 513
Size: 658 517 3074
Fragments arranged by size:
3074 939 658 552 540 517 237 219
166 161 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2557 2681 3218 3412 5102 5119 5272
Size: 1255 977 124 537 194 1690 17 153
Cuts at: 5272 5457 5902 325
Size: 185 445 1498
Fragments arranged by size:
1690 1498 1255 977 537 445 194 185
153 124 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 2565 2919
Size: 274 80 33 51 1443 357 231 354
Cuts at: 2919 3060 3614 3894 5321 7033 96
Size: 141 554 280 1427 1712 138
Fragments arranged by size:
1712 1443 1427 554 357 354 280 274
231 141 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 7035 7067 3674
Size: 32 3329 32 3682
Fragments arranged by size:
3682 3329 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7075
XbaI T'CTAG_A
Cuts at: 4411 4411
Size: 7075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3973 4228 3973
Size: 255 6820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7075
XmaI C'CCGG_G
Cuts at: 1870 3557 4155 4400 1870
Size: 1687 598 245 4545
Fragments arranged by size:
4545 1687 598 245
XmnI GAAnn'nnTTC
Cuts at: 3048 4034 4350 5204 3048
Size: 986 316 854 4919
Fragments arranged by size:
4919 986 854 316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4883 276
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SwaI