Restriction map for
pFN21AB3158
(Circular) (Six-base) MAPSORT of: pFN21AB3158.seq Check: 2611 from: 1 to: 7477
pFN21AB3158 7477 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5287 278
Size: 53 83 186 936 3751 2468
Fragments arranged by size:
3751 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2014 4233 4820 1937
Size: 77 2219 587 4594
Fragments arranged by size:
4594 2219 587 77
Acc65I G'GTAC_C
Cuts at: 4798 4798
Size: 7477
AclI AA'CG_TT
Cuts at: 1563 5604 5977 1563
Size: 4041 373 3063
Fragments arranged by size:
4041 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2263 2334 2476 3838 4291 5540 6924
Size: 442 189 71 142 1362 453 1249 1384
Cuts at: 6924 7192 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1362 1249 453 442 268 189
142 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7280
AflIII A'CryG_T
Cuts at: 1203 6376 7353 1203
Size: 5173 977 1327
Fragments arranged by size:
5173 1327 977
AgeI A'CCGG_T
Cuts at: 2249 6308 2249
Size: 4059 3418
AhdI GACnn_n'nnGTC
Cuts at: 2227 6206 2227
Size: 3979 3498
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7477
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3075 3107 3075
Size: 32 7445
AlwNI CAG_nnn'CTG
Cuts at: 3920 5220 6792 3920
Size: 1300 1572 4605
Fragments arranged by size:
4605 1572 1300
ApaLI G'TGCA_C
Cuts at: 5534 6690 5534
Size: 1156 6321
ApoI r'AATT_y
Cuts at: 1493 3690 4696 4786 5012 5164 7273 1493
Size: 2197 1006 90 226 152 2109 1697
Fragments arranged by size:
2197 2109 1697 1006 226 152 90
AseI AT'TA_AT
Cuts at: 160 1032 2646 2862 4512 6031 160
Size: 872 1614 216 1650 1519 1606
Fragments arranged by size:
1650 1614 1606 1519 872 216
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7477
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2292 4412 4802 1870
Size: 73 156 193 2120 390 4545
Fragments arranged by size:
4545 2120 390 193 156 73
AvrII C'CTAG_G
Cuts at: 4292 4292
Size: 7477
BamHI G'GATC_C
Cuts at: 4807 5174 4807
Size: 367 7110
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2930 4798 6253 618
Size: 325 217 627 1143 1868 1455 1842
Fragments arranged by size:
1868 1842 1455 1143 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4374 4796 1391
Size: 2983 422 4072
Fragments arranged by size:
4072 2983 422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6506
BbvCI CC'TCA_GC
Cuts at: 2799 4004 2799
Size: 1205 6272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5668 5702 1903
Size: 34 3731 34 3678
Fragments arranged by size:
3731 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5369 6579 5369
Size: 1210 6267
BclI T'GATC_A
Cuts at: 1548 4399 1548
Size: 2851 4626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3797 6088 136
Size: 107 122 71 631 605 2125 2291 1525
Fragments arranged by size:
2291 2125 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2562 7472 2562
Size: 4910 2567
BlpI GC'TnA_GC
Cuts at: 4894 4894
Size: 7477
Bme1580I G_kGCm'C
Cuts at: 1790 2933 5538 6694 1790
Size: 1143 2605 1156 2573
Fragments arranged by size:
2605 2573 1156 1143
BmgBI CAC'GTC
Cuts at: 1318 2701 1318
Size: 1383 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5208 6166 442
Size: 416 53 83 399 24 3791 958 1753
Fragments arranged by size:
3791 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7477
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3583 3583
Size: 7477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3075 3116 6137 1239
Size: 1836 41 3021 2579
Fragments arranged by size:
3021 2579 1836 41
Bpu10I CC'TnA_GC
Cuts at: 2355 2799 4004 2355
Size: 444 1205 5828
Fragments arranged by size:
5828 1205 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5602 6467 6765 5602
Size: 865 298 6314
Fragments arranged by size:
6314 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2817 3386 6140 882
Size: 617 1318 569 2754 2219
Fragments arranged by size:
2754 2219 1318 617 569
BsaAI yAC'GTr
Cuts at: 493 1204 2314 5268 493
Size: 711 1110 2954 2702
Fragments arranged by size:
2954 2702 1110 711
BsaBI GATnn'nnATC
Cuts at: 2516 7471 2516
Size: 4955 2522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2011 5284 5666 6301
Size: 53 83 186 936 478 3273 382 635
Cuts at: 6301 275
Size: 1451
Fragments arranged by size:
3273 1451 936 635 478 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2249 5910 6308 6582 6729 7140 2249
Size: 3661 398 274 147 411 2586
Fragments arranged by size:
3661 2586 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 3075 3105 4273
Size: 30 165 30 960 30 682 30 1168
Cuts at: 4273 4303 1178
Size: 30 4352
Fragments arranged by size:
4352 1168 960 682 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2419 2422 2720 3632 4081 4131 4535 1186
Size: 1233 3 298 912 449 50 404 4128
Fragments arranged by size:
4128 1233 912 449 404 298 50 3
BseYI C'CCAG_C
Cuts at: 6680 7176 6680
Size: 496 6981
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 1163
Size: 573 591 6313
Fragments arranged by size:
6313 591 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4927 5688 6716 1540
Size: 275 186 2926 761 1028 2301
Fragments arranged by size:
2926 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 5538 5623 6694 2166
Size: 3372 85 1071 2949
Fragments arranged by size:
3372 2949 1071 85
BsmI GAATG_Cn'
Cuts at: 2923 4258 4988 5081 2923
Size: 1335 730 93 5319
Fragments arranged by size:
5319 1335 730 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2933 4374 4796 5538 5623 6694
Size: 399 376 767 1441 422 742 85 1071
Cuts at: 6694 1391
Size: 2174
Fragments arranged by size:
2174 1441 1071 767 742 422 399 376
85
BspEI T'CCGG_A
Cuts at: 7140 7140
Size: 7477
BspHI T'CATG_A
Cuts at: 5366 7096 5366
Size: 1730 5747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2196 4832 844
Size: 1214 138 2636 3489
Fragments arranged by size:
3489 2636 1214 138
BsrBI CCG'CTC
Cuts at: 1394 1823 2331 2412 5364 1394
Size: 429 508 81 2952 3507
Fragments arranged by size:
3507 2952 508 429 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 3273 4349 5972 6146 65
Size: 1183 2025 1076 1623 174 1396
Fragments arranged by size:
2025 1623 1396 1183 1076 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2249 6121 6308 1700
Size: 110 439 3872 187 2869
Fragments arranged by size:
3872 2869 439 187 110
BsrGI T'GTAC_A
Cuts at: 96 2130 96
Size: 2034 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7477
BssSI C'ACGA_G
Cuts at: 5537 6549 5537
Size: 1012 6465
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7477
BstBI TT'CG_AA
Cuts at: 6281 6281
Size: 7477
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7477
BstXI CCAn_nnnn'nTGG
Cuts at: 4435 4630 4435
Size: 195 7282
BstYI r'GATC_y
Cuts at: 1972 2562 3295 4807 5174 5559 5576 7028 7114
Size: 590 733 1512 367 385 17 1452 86
Cuts at: 7114 7126 7472 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1512 1452 733 590 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2481 2481
Size: 7477
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2680 513
Size: 552 939 150 526 5310
Fragments arranged by size:
5310 939 552 526 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4361 487
Size: 683 56 3135 3603
Fragments arranged by size:
3603 3135 683 56
BtsI GCAGTG_nn'
Cuts at: 2836 3153 4993 5787 5807 2836
Size: 317 1840 794 20 4506
Fragments arranged by size:
4506 1840 794 317 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2805 2840 529
Size: 35 2241 35 5166
Fragments arranged by size:
5166 2241 35 35
DraI TTT'AAA
Cuts at: 4781 4781
Size: 7477
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6484 809
Size: 507 5168 1802
Fragments arranged by size:
5168 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2305 2607
Size: 54 1425 50 275 18 348 127 302
Cuts at: 2607 4924 5813 7442 8
Size: 2317 889 1629 43
Fragments arranged by size:
2317 1629 1425 889 348 302 275 127
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4924 1537
Size: 275 3112 4090
Fragments arranged by size:
4090 3112 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2885 3552 3717 4279 4338 5407 1353
Size: 728 804 667 165 562 59 1069 3423
Fragments arranged by size:
3423 1069 804 728 667 562 165 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6064 6438 6584 387
Size: 1064 138 4475 374 146 1280
Fragments arranged by size:
4475 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2263 2334 2476 3075 3116 3838
Size: 393 442 189 71 142 599 41 722
Cuts at: 3838 4291 5540 6137 6924 7192 1239
Size: 453 1249 597 787 268 1524
Fragments arranged by size:
1524 1249 787 722 599 597 453 442
393 268 189 142 71 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2180 2480 2141
Size: 39 300 7138
Fragments arranged by size:
7138 300 39
EcoO109I rG'GnC_Cy
Cuts at: 2084 2185 2374 2575 3647 4289 2084
Size: 101 189 201 1072 642 5272
Fragments arranged by size:
5272 1072 642 201 189 101
EcoRI G'AATT_C
Cuts at: 3690 4786 5164 3690
Size: 1096 378 6003
Fragments arranged by size:
6003 1096 378
EcoRV GAT'ATC
Cuts at: 4126 4126
Size: 7477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3512 3512
Size: 7477
FspI TGC'GCA
Cuts at: 5983 5983
Size: 7477
HaeII r_GCGC'y
Cuts at: 1678 6624 1678
Size: 4946 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2315 2347 2795
Size: 32 135 32 641 32 70 32 448
Cuts at: 2795 2827 2965 2997 3456 3488 3644 3676 3732
Size: 32 138 32 459 32 156 32 56
Cuts at: 3732 3764 4273 4305 6118 6150 6192 6224 1373
Size: 32 509 32 1813 32 42 32 2626
Fragments arranged by size:
2626 1813 641 509 459 448 156 138
135 70 56 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3265 3319 3760 4234 4821 5067
Size: 988 281 1327 54 441 474 587 246
Cuts at: 5067 669
Size: 3079
Fragments arranged by size:
3079 1327 988 587 474 441 281 246
54
HindIII A'AGCT_T
Cuts at: 748 4612 748
Size: 3864 3613
HpaI GTT'AAC
Cuts at: 3265 3319 5067 3265
Size: 54 1748 5675
Fragments arranged by size:
5675 1748 54
KpnI G_GTAC'C
Cuts at: 4802 4802
Size: 7477
MfeI C'AATT_G
Cuts at: 2869 2937 5076 2869
Size: 68 2139 5270
Fragments arranged by size:
5270 2139 68
MluI A'CGCG_T
Cuts at: 7353 7353
Size: 7477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3792 6591 6775 1305
Size: 2487 2799 184 2007
Fragments arranged by size:
2799 2487 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2307 2609 10
Size: 54 1425 343 475 302 4878
Fragments arranged by size:
4878 1425 475 343 302 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3042 3488 4247 4433 4640 5435 5794
Size: 648 1876 446 759 186 207 795 359
Cuts at: 5794 5953 518
Size: 159 2042
Fragments arranged by size:
2042 1876 795 759 648 446 359 207
186 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2156 2236 2945 3712 4893 5570 6718
Size: 488 62 80 709 767 1181 677 1148
Cuts at: 6718 6963 1606
Size: 245 2120
Fragments arranged by size:
2120 1181 1148 767 709 677 488 245
80 62
NcoI C'CATG_G
Cuts at: 513 1065 2004 2680 513
Size: 552 939 676 5310
Fragments arranged by size:
5310 939 676 552
NdeI CA'TA_TG
Cuts at: 387 4740 387
Size: 4353 3124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7477
NotI GC'GGCC_GC
Cuts at: 4924 4924
Size: 7477
NspI r_CATG'y
Cuts at: 1132 1229 4835 6380 1132
Size: 97 3606 1545 2229
Fragments arranged by size:
3606 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4683 1348
Size: 3335 4142
PciI A'CATG_T
Cuts at: 6376 6376
Size: 7477
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2956 3887 1108
Size: 1848 931 4698
Fragments arranged by size:
4698 1848 931
PfoI T'CCnGG_A
Cuts at: 2577 2577
Size: 7477
PmeI GTTT'AAAC
Cuts at: 4781 4781
Size: 7477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3075 3107 5546 5578 7059 7091 3075
Size: 32 2439 32 1481 32 3461
Fragments arranged by size:
3461 2439 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2084 2185 2575 3647 4289 2084
Size: 101 390 1072 642 5272
Fragments arranged by size:
5272 1072 642 390 101
PshAI GACnn'nnGTC
Cuts at: 1613 2448 3828 1613
Size: 835 1380 5262
Fragments arranged by size:
5262 1380 835
PsiI TTA'TAA
Cuts at: 5047 5047
Size: 7477
PstI C_TGCA'G
Cuts at: 830 2212 4359 4829 830
Size: 1382 2147 470 3478
Fragments arranged by size:
3478 2147 1382 470
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7477
PvuII CAG'CTG
Cuts at: 2094 2236 2945 3712 2094
Size: 142 709 767 5859
Fragments arranged by size:
5859 767 709 142
SacII CC_GC'GG
Cuts at: 2157 2157
Size: 7477
SalI G'TCGA_C
Cuts at: 1936 4232 4819 1936
Size: 2296 587 4594
Fragments arranged by size:
4594 2296 587
SanDI GG'GwC_CC
Cuts at: 2084 2575 2084
Size: 491 6986
SbfI CC_TGCA'GG
Cuts at: 4829 4829
Size: 7477
ScaI AGT'ACT
Cuts at: 4185 5725 7437 4185
Size: 1540 1712 4225
Fragments arranged by size:
4225 1712 1540
SfcI C'TryA_G
Cuts at: 826 1045 2041 2208 2922 3419 3600 4095 4355
Size: 219 996 167 714 497 181 495 260
Cuts at: 4355 4825 5960 6641 6832 826
Size: 470 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 714 681 497 495 470
260 219 191 181 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7477
SgrAI Cr'CCGG_yG
Cuts at: 1700 2249 1700
Size: 549 6928
SmaI CCC'GGG
Cuts at: 1872 2294 4804 1872
Size: 422 2510 4545
Fragments arranged by size:
4545 2510 422
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5581 6482 6744 820
Size: 197 926 3638 901 262 1553
Fragments arranged by size:
3638 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5268 493
Size: 4775 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7477
SphI G_CATG'C
Cuts at: 1132 4835 1132
Size: 3703 3774
SspI AAT'ATT
Cuts at: 5 52 3383 4774 5401 5
Size: 47 3331 1391 627 2081
Fragments arranged by size:
3331 2081 1391 627 47
StuI AGG'CCT
Cuts at: 1517 4297 1517
Size: 2780 4697
StyI C'CwwG_G
Cuts at: 513 1065 2004 2680 4292 4916 513
Size: 552 939 676 1612 624 3074
Fragments arranged by size:
3074 1612 939 676 624 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2738 3586 3732 3790 5504 5521 5674
Size: 1255 1158 848 146 58 1714 17 153
Cuts at: 5674 5859 6304 325
Size: 185 445 1498
Fragments arranged by size:
1714 1498 1255 1158 848 445 185 153
146 58 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 3735 4183
Size: 274 80 33 51 1443 153 1605 448
Cuts at: 4183 4705 5723 7435 96
Size: 522 1018 1712 138
Fragments arranged by size:
1712 1605 1443 1018 522 448 274 153
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 7437 7469 2363
Size: 32 5042 32 2371
Fragments arranged by size:
5042 2371 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3680 4460 1316
Size: 2364 780 4333
Fragments arranged by size:
4333 2364 780
XbaI T'CTAG_A
Cuts at: 4120 4813 4120
Size: 693 6784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3403 3403
Size: 7477
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7477
XmaI C'CCGG_G
Cuts at: 1870 2292 4802 1870
Size: 422 2510 4545
Fragments arranged by size:
4545 2510 422
XmnI GAAnn'nnTTC
Cuts at: 3876 5606 3876
Size: 1730 5747
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5285 276
Size: 53 83 186 936 3751 2468
Fragments arranged by size:
3751 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI
SwaI