Restriction map for
pFN21AB3144
(Circular) (Six-base) MAPSORT of: pFN21AB3144.seq Check: 1418 from: 1 to: 6430
pFN21AB3144 6430 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2452 2583 2587 2452
Size: 131 4 6295
Fragments arranged by size:
6295 131 4
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 3431 4557 4930 1563
Size: 1868 1126 373 3063
Fragments arranged by size:
3063 1868 1126 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3191 3648 3694 4493 5877 6145 1632
Size: 1559 457 46 799 1384 268 1917
Fragments arranged by size:
1917 1559 1384 799 457 268 46
AfeI AGC'GCT
Cuts at: 3218 3218
Size: 6430
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3498 1184
Size: 2314 4116
AlwNI CAG_nnn'CTG
Cuts at: 2611 2686 4173 5745 2611
Size: 75 1487 1572 3296
Fragments arranged by size:
3296 1572 1487 75
ApaI G_GGCC'C
Cuts at: 2060 2374 2631 2670 2060
Size: 314 257 39 5820
Fragments arranged by size:
5820 314 257 39
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 3282 3739 3965 4117 6226 1493
Size: 1789 457 226 152 2109 1697
Fragments arranged by size:
2109 1789 1697 457 226 152
AscI GG'CGCG_CC
Cuts at: 2585 2585
Size: 6430
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2061 2624 3755 1870
Size: 73 85 33 563 1131 4545
Fragments arranged by size:
4545 1131 563 85 73 33
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2518 2861 3161 3620 3751
Size: 325 217 627 731 343 300 459 131
Cuts at: 3751 5206 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 731 627 459 343 325 300
217 131
BanII G_rGCy'C
Cuts at: 1391 2060 2205 2306 2374 2631 2670 3749 1391
Size: 669 145 101 68 257 39 1079 4072
Fragments arranged by size:
4072 1079 669 257 145 101 68 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2283 928
Size: 971 384 5075
Fragments arranged by size:
5075 971 384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2329 2363 4621 4655 1903
Size: 34 392 34 2258 34 3678
Fragments arranged by size:
3678 2258 392 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 2420 2699
Size: 107 122 71 631 605 433 315 279
Cuts at: 2699 2724 5041 136
Size: 25 2317 1525
Fragments arranged by size:
2317 1525 631 605 433 315 279 122
107 71 25
BglII A'GATC_T
Cuts at: 3000 6425 3000
Size: 3425 3005
BlpI GC'TnA_GC
Cuts at: 2109 2117 2843 2976 3847 2109
Size: 8 726 133 871 4692
Fragments arranged by size:
4692 871 726 133 8
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2374 2523 2631 2670 2680 3116 3164
Size: 270 314 149 108 39 10 436 48
Cuts at: 3164 3625 4491 5647 1790
Size: 461 866 1156 2573
Fragments arranged by size:
2573 1156 866 461 436 314 270 149
108 48 39 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2427 3119 4161
Size: 416 53 83 399 24 1010 692 1042
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1753 1042 1010 958 692 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2573 2837 3251 3272 3321 3587 5090
Size: 1051 283 264 414 21 49 266 1503
Cuts at: 5090 1239
Size: 2579
Fragments arranged by size:
2579 1503 1051 414 283 266 264 49
21
Bpu10I CC'TnA_GC
Cuts at: 2514 2514
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2215 2519 3365 4555 5420 5718 2215
Size: 304 846 1190 865 298 2927
Fragments arranged by size:
2927 1190 865 846 304 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3002 3540 5093 882
Size: 617 1503 538 1553 2219
Fragments arranged by size:
2219 1553 1503 617 538
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2472 4237 4619 5254
Size: 53 83 186 936 939 1765 382 635
Cuts at: 5254 275
Size: 1451
Fragments arranged by size:
1765 1451 939 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2137 4863 5261 5535 5682 6093 2137
Size: 2726 398 274 147 411 2474
Fragments arranged by size:
2726 2474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2862 2892 1178
Size: 30 165 30 1459 30 4716
Fragments arranged by size:
4716 1459 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 3419 3422 3599 3602 3605 3737 1186
Size: 913 1320 3 177 3 3 132 3879
Fragments arranged by size:
3879 1320 913 177 132 3 3 3
BseYI C'CCAG_C
Cuts at: 2269 2480 2679 2882 2971 3521 5633 6129 2269
Size: 211 199 203 89 550 2112 496 2570
Fragments arranged by size:
2570 2112 550 496 211 203 199 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2424 2561 3468 3497 1163
Size: 573 688 137 907 29 4096
Fragments arranged by size:
4096 907 688 573 137 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2100 3880 4641 5669 1540
Size: 275 186 18 81 1780 761 1028 2301
Fragments arranged by size:
2301 1780 1028 761 275 186 81 18
BsiHKAI G_wGCw'C
Cuts at: 2205 2535 4491 4576 5647 2205
Size: 330 1956 85 1071 2988
Fragments arranged by size:
2988 1956 1071 330 85
BsmI GAATG_Cn'
Cuts at: 3466 3941 4034 3466
Size: 475 93 5862
Fragments arranged by size:
5862 475 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2479 2479
Size: 6430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2205 2306 2374 2523 2535 2631
Size: 399 270 145 101 68 149 12 96
Cuts at: 2631 2670 2680 3116 3164 3625 3749 4491 4576
Size: 39 10 436 48 461 124 742 85
Cuts at: 4576 5647 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 461 436 399 270 149
145 124 101 96 85 68 48 39
12 10
BspEI T'CCGG_A
Cuts at: 2137 6093 2137
Size: 3956 2474
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 2583 2587 3460 3519 3785 844
Size: 1608 131 4 873 59 266 3489
Fragments arranged by size:
3489 1608 873 266 131 59 4
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 1394
Size: 429 2494 3507
Fragments arranged by size:
3507 2494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 2589 2714 3237 5074 5261 1700
Size: 110 285 494 125 523 1837 187 2869
Fragments arranged by size:
2869 1837 523 494 285 187 125 110
BsrGI T'GTAC_A
Cuts at: 96 2919 96
Size: 2823 3607
BssHII G'CGCG_C
Cuts at: 1925 2585 1925
Size: 660 5770
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2365 2967 794
Size: 1571 602 4257
Fragments arranged by size:
4257 1571 602
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstXI CCAn_nnnn'nTGG
Cuts at: 2464 3229 3314 2464
Size: 765 85 5580
Fragments arranged by size:
5580 765 85
BstYI r'GATC_y
Cuts at: 1972 3000 3309 3760 4127 4512 4529 5981 6067
Size: 1028 309 451 367 385 17 1452 86
Cuts at: 6067 6079 6425 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1028 451 385 367 346 309
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2697 2697
Size: 6430
BtgI C'CryG_G
Cuts at: 513 1065 2418 2745 3313 513
Size: 552 1353 327 568 3630
Fragments arranged by size:
3630 1353 568 552 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 2246 2684 2734 3632 3946 4740 4760 2246
Size: 438 50 898 314 794 20 3916
Fragments arranged by size:
3916 898 794 438 314 50 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2097 2937
Size: 54 1425 50 275 18 186 81 840
Cuts at: 2937 3354 3877 4766 6395 8
Size: 417 523 889 1629 43
Fragments arranged by size:
1629 1425 889 840 523 417 275 186
81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2097 3877 1537
Size: 275 204 81 1780 4090
Fragments arranged by size:
4090 1780 275 204 81
EarI CTCTTCn'nnn_
Cuts at: 1353 3667 3714 4360 1353
Size: 2314 47 646 3423
Fragments arranged by size:
3423 2314 646 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5017 5391 5537 387
Size: 1064 138 3428 374 146 1280
Fragments arranged by size:
3428 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2203 2203
Size: 6430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2573 2837 3191 3251 3272 3321
Size: 393 658 283 264 354 60 21 49
Cuts at: 3321 3587 3648 3694 4493 5090 5877 6145 1239
Size: 266 61 46 799 597 787 268 1524
Fragments arranged by size:
1524 799 787 658 597 393 354 283
268 266 264 61 60 49 46 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2579 2898 3029 3254 2579
Size: 319 131 225 5755
Fragments arranged by size:
5755 319 225 131
EcoO109I rG'GnC_Cy
Cuts at: 2056 2057 2310 2647 2666 3022 3449 3538 2056
Size: 1 253 337 19 356 427 89 4948
Fragments arranged by size:
4948 427 356 337 253 89 19 1
EcoRI G'AATT_C
Cuts at: 3282 3739 4117 3282
Size: 457 378 5595
Fragments arranged by size:
5595 457 378
FseI GG_CCGG'CC
Cuts at: 2099 2099
Size: 6430
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2753 3220 5577 1678
Size: 1075 467 2357 2531
Fragments arranged by size:
2531 2357 1075 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2862 2894 5071 5103 5145
Size: 32 135 32 1290 32 2177 32 42
Cuts at: 5145 5177 1373
Size: 32 2626
Fragments arranged by size:
2626 2177 1290 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 5544 5728 1305
Size: 756 3483 184 2007
Fragments arranged by size:
3483 2007 756 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2939 3356 10
Size: 54 1425 343 1107 417 3084
Fragments arranged by size:
3084 1425 1107 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 3578 4388 4747 4906 518
Size: 648 1296 1116 810 359 159 2042
Fragments arranged by size:
2042 1296 1116 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2420 2975 3394 3846 4523 5671 5916
Size: 507 307 555 419 452 677 1148 245
Cuts at: 5916 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 555 507 452 419 307
245
NaeI GCC'GGC
Cuts at: 2097 2591 3239 2097
Size: 494 648 5288
Fragments arranged by size:
5288 648 494
NcoI C'CATG_G
Cuts at: 513 1065 2745 3313 513
Size: 552 1680 568 3630
Fragments arranged by size:
3630 1680 568 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2095 2589 3237 2095
Size: 494 648 5288
Fragments arranged by size:
5288 648 494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3788 5333 1132
Size: 97 2559 1545 2229
Fragments arranged by size:
2559 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2523 1348
Size: 1175 5255
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6430
PfoI T'CCnGG_A
Cuts at: 2144 2306 2144
Size: 162 6268
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2310 3449 3538 2310
Size: 1139 89 5202
Fragments arranged by size:
5202 1139 89
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2056 2370 2627 2666 2056
Size: 314 257 39 5820
Fragments arranged by size:
5820 314 257 39
PspXI vC'TCGA_Gb
Cuts at: 2061 2061
Size: 6430
PstI C_TGCA'G
Cuts at: 830 2253 2299 2580 2877 3204 3276 3303 3782
Size: 1423 46 281 297 327 72 27 479
Cuts at: 3782 830
Size: 3478
Fragments arranged by size:
3478 1423 479 327 297 281 72 46
27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2113 2975 3394 2113
Size: 862 419 5149
Fragments arranged by size:
5149 862 419
SacI G_AGCT'C
Cuts at: 2205 2205
Size: 6430
SacII CC_GC'GG
Cuts at: 2421 2421
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 2249 2295 2576 2873 3200 3272 3299
Size: 219 1204 46 281 297 327 72 27
Cuts at: 3299 3778 4913 5594 5785 826
Size: 479 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 479 327 297 281
219 191 72 46 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 3757 1872
Size: 1885 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2061 2230 2534 3380 4534 5435
Size: 197 926 118 169 304 846 1154 901
Cuts at: 5435 5697 820
Size: 262 1553
Fragments arranged by size:
1553 1154 926 901 846 304 262 197
169 118
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 3176 1517
Size: 1659 4771
StyI C'CwwG_G
Cuts at: 513 1065 2745 3313 3351 3684 3869 513
Size: 552 1680 568 38 333 185 3074
Fragments arranged by size:
3074 1680 568 552 333 185 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3026 4457 4474 4627 4812 5257 325
Size: 1255 1446 1431 17 153 185 445 1498
Fragments arranged by size:
1498 1446 1431 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2919 4676 6388
Size: 274 80 33 51 1443 942 1757 1712
Cuts at: 6388 96
Size: 138
Fragments arranged by size:
1757 1712 1443 942 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6390 6422 6390
Size: 32 6398
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2743 3529 2743
Size: 786 5644
XhoI C'TCGA_G
Cuts at: 1943 2061 1943
Size: 118 6312
XmaI C'CCGG_G
Cuts at: 1870 3755 1870
Size: 1885 4545
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI FspAI KasI NarI NruI
NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI