Restriction map for
pFN21AB3134
(Circular) (Six-base) MAPSORT of: pFN21AB3134.seq Check: 4536 from: 1 to: 8395
pFN21AB3134 8395 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3697 3697
Size: 8395
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6205 278
Size: 53 83 186 936 4669 2468
Fragments arranged by size:
4669 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5738 1937
Size: 3801 4594
Acc65I G'GTAC_C
Cuts at: 2029 5716 2029
Size: 3687 4708
AclI AA'CG_TT
Cuts at: 1563 6522 6895 1563
Size: 4959 373 3063
Fragments arranged by size:
4959 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 6458 7842 8110 1632
Size: 680 4146 1384 268 1917
Fragments arranged by size:
4146 1917 1384 680 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8198
AflIII A'CryG_T
Cuts at: 1203 7294 8271 1203
Size: 6091 977 1327
Fragments arranged by size:
6091 1327 977
AgeI A'CCGG_T
Cuts at: 7226 7226
Size: 8395
AhdI GACnn_n'nnGTC
Cuts at: 7124 7124
Size: 8395
AleI CACnn'nnGTG
Cuts at: 1166 5414 1166
Size: 4248 4147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 4713 4745 5015 5047 3371
Size: 32 1310 32 270 32 6719
Fragments arranged by size:
6719 1310 270 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2747 4541 5374 6138 7710 2747
Size: 1794 833 764 1572 3432
Fragments arranged by size:
3432 1794 1572 833 764
ApaLI G'TGCA_C
Cuts at: 6452 7608 6452
Size: 1156 7239
ApoI r'AATT_y
Cuts at: 1493 2230 2292 4353 4760 5704 5930 6082 8191
Size: 737 62 2061 407 944 226 152 2109
Cuts at: 8191 1493
Size: 1697
Fragments arranged by size:
2109 2061 1697 944 737 407 226 152
62
AseI AT'TA_AT
Cuts at: 160 1032 4077 6949 160
Size: 872 3045 2872 1606
Fragments arranged by size:
3045 2872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8395
AvaI C'yCGr_G
Cuts at: 1870 1943 3548 5244 5720 1870
Size: 73 1605 1696 476 4545
Fragments arranged by size:
4545 1696 1605 476 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4591 4624 4591
Size: 33 8362
BamHI G'GATC_C
Cuts at: 3497 4203 5725 6092 3497
Size: 706 1522 367 5800
Fragments arranged by size:
5800 1522 706 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3118 5716 7171 618
Size: 325 217 627 242 1089 2598 1455 1842
Fragments arranged by size:
2598 1842 1455 1089 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 3288 3316 3897 5259 5714 1391
Size: 1897 28 581 1362 455 4072
Fragments arranged by size:
4072 1897 1362 581 455 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2808 3684 3970 4266 5331 928
Size: 971 909 876 286 296 1065 3992
Fragments arranged by size:
3992 1065 971 909 876 296 286
BbvCI CC'TCA_GC
Cuts at: 3348 3483 3665 4863 5265 3348
Size: 135 182 1198 402 6478
Fragments arranged by size:
6478 1198 402 182 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6586 6620 1903
Size: 34 50 34 4565 34 3678
Fragments arranged by size:
4565 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6287 7497 6287
Size: 1210 7185
BclI T'GATC_A
Cuts at: 1548 2092 2151 3489 1548
Size: 544 59 1338 6454
Fragments arranged by size:
6454 1338 544 59
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7006 136
Size: 107 122 71 631 605 5334 1525
Fragments arranged by size:
5334 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5064 5119 5515 8390 5064
Size: 55 396 2875 5069
Fragments arranged by size:
5069 2875 396 55
BlpI GC'TnA_GC
Cuts at: 2400 5253 5812 2400
Size: 2853 559 4983
Fragments arranged by size:
4983 2853 559
Bme1580I G_kGCm'C
Cuts at: 1790 3837 6456 7612 1790
Size: 2047 2619 1156 2573
Fragments arranged by size:
2619 2573 2047 1156
BmgBI CAC'GTC
Cuts at: 1318 3607 3789 1318
Size: 2289 182 5924
Fragments arranged by size:
5924 2289 182
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 4486 6126
Size: 416 53 83 399 24 777 2292 1640
Cuts at: 6126 7084 442
Size: 958 1753
Fragments arranged by size:
2292 1753 1640 958 777 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4911 4919 1056
Size: 3855 8 4532
Fragments arranged by size:
4532 3855 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5064 5064
Size: 8395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4127 5363 5396 7055 1239
Size: 2888 1236 33 1659 2579
Fragments arranged by size:
2888 2579 1659 1236 33
Bpu10I CC'TnA_GC
Cuts at: 2388 3348 3483 3665 4863 5039 5265 5363 2388
Size: 960 135 182 1198 176 226 98 5420
Fragments arranged by size:
5420 1198 960 226 182 176 135 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2453 3242 3452 4015 4022 5144 6520 7385
Size: 300 789 210 563 7 1122 1376 865
Cuts at: 7385 7683 2153
Size: 298 2865
Fragments arranged by size:
2865 1376 1122 865 789 563 300 298
210 7
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3410 7058 882
Size: 617 1911 3648 2219
Fragments arranged by size:
3648 2219 1911 617
BsaAI yAC'GTr
Cuts at: 493 1204 6186 493
Size: 711 4982 2702
Fragments arranged by size:
4982 2702 711
BsaBI GATnn'nnATC
Cuts at: 2153 2877 5315 8389 2153
Size: 724 2438 3074 2159
Fragments arranged by size:
3074 2438 2159 724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2194 6202 6584 7219
Size: 53 83 186 936 661 4008 382 635
Cuts at: 7219 275
Size: 1451
Fragments arranged by size:
4008 1451 936 661 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2334 4413 6828 7226 7500 7647 8058 2334
Size: 2079 2415 398 274 147 411 2671
Fragments arranged by size:
2671 2415 2079 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3371 3401 4192 4222 4713
Size: 30 165 30 1968 30 791 30 491
Cuts at: 4713 4743 5015 5045 5336 5366 5679 5709 1178
Size: 30 272 30 291 30 313 30 3864
Fragments arranged by size:
3864 1968 791 491 313 291 272 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3320 3365 3512 3585 5570 1186
Size: 2134 45 147 73 1985 4011
Fragments arranged by size:
4011 2134 1985 147 73 45
BseYI C'CCAG_C
Cuts at: 3870 4324 7598 8094 3870
Size: 454 3274 496 4171
Fragments arranged by size:
4171 3274 496 454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3038 3368 4502 4835 1163
Size: 573 1302 330 1134 333 4723
Fragments arranged by size:
4723 1302 1134 573 333 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5845 6606 7634 1540
Size: 275 186 3844 761 1028 2301
Fragments arranged by size:
3844 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 4119 5259 6456 6541 7612 3897
Size: 222 1140 1197 85 1071 4680
Fragments arranged by size:
4680 1197 1140 1071 222 85
BsmI GAATG_Cn'
Cuts at: 2640 3642 5906 5999 2640
Size: 1002 2264 93 5036
Fragments arranged by size:
5036 2264 1002 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3288 3316 3837 3897 4119 5259 5714
Size: 399 1498 28 521 60 222 1140 455
Cuts at: 5714 6456 6541 7612 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1498 1140 1071 742 521 455 399
222 85 60 28
BspEI T'CCGG_A
Cuts at: 4413 8058 4413
Size: 3645 4750
BspHI T'CATG_A
Cuts at: 3210 5670 6284 8014 3210
Size: 2460 614 1730 3591
Fragments arranged by size:
3591 2460 1730 614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2041 2395 3390 3697 4815 5750 844
Size: 1197 354 995 307 1118 935 3489
Fragments arranged by size:
3489 1197 1118 995 935 354 307
BsrBI CCG'CTC
Cuts at: 1394 1823 6282 1394
Size: 429 4459 3507
Fragments arranged by size:
4459 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4942 5259 6890 7064 65
Size: 1183 3694 317 1631 174 1396
Fragments arranged by size:
3694 1631 1396 1183 317 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3111 7039 7226 1700
Size: 110 1301 3928 187 2869
Fragments arranged by size:
3928 2869 1301 187 110
BsrGI T'GTAC_A
Cuts at: 96 3163 96
Size: 3067 5328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8395
BssSI C'ACGA_G
Cuts at: 3851 6455 7467 3851
Size: 2604 1012 4779
Fragments arranged by size:
4779 2604 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4016 4541 794
Size: 3222 525 4648
Fragments arranged by size:
4648 3222 525
BstBI TT'CG_AA
Cuts at: 4765 7199 4765
Size: 2434 5961
BstEII G'GTnAC_C
Cuts at: 1184 2126 1184
Size: 942 7453
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 2500
Size: 8395
BstYI r'GATC_y
Cuts at: 1972 2915 3497 3988 4203 4940 5064 5119 5515
Size: 943 582 491 215 737 124 55 396
Cuts at: 5515 5725 6092 6477 6494 7946 8032 8044 8390
Size: 210 367 385 17 1452 86 12 346
Cuts at: 8390 1972
Size: 1977
Fragments arranged by size:
1977 1452 943 737 582 491 396 385
367 346 215 210 124 86 55 17
12
Bsu36I CC'TnA_GG
Cuts at: 3303 3303
Size: 8395
BtgI C'CryG_G
Cuts at: 513 1065 3450 3510 4056 4497 4689 5502 513
Size: 552 2385 60 546 441 192 813 3406
Fragments arranged by size:
3406 2385 813 552 546 441 192 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5663 487
Size: 683 56 4437 3219
Fragments arranged by size:
4437 3219 683 56
BtsI GCAGTG_nn'
Cuts at: 5198 5911 6705 6725 5198
Size: 713 794 20 6868
Fragments arranged by size:
6868 794 713 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8360
DraI TTT'AAA
Cuts at: 4222 5699 4222
Size: 1477 6918
DraIII CAC_nnn'GTG
Cuts at: 4525 4525
Size: 8395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7402 809
Size: 507 6086 1802
Fragments arranged by size:
6086 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2502 3711 5842
Size: 54 1425 50 275 18 672 1209 2131
Cuts at: 5842 6731 8360 8
Size: 889 1629 43
Fragments arranged by size:
2131 1629 1425 1209 889 672 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5842 1537
Size: 275 4030 4090
Fragments arranged by size:
4090 4030 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4469 6325 1353
Size: 3116 1856 3423
Fragments arranged by size:
3423 3116 1856
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2504 6982 7356 7502 387
Size: 1064 138 915 4478 374 146 1280
Fragments arranged by size:
4478 1280 1064 915 374 146 138
EcoICRI GAG'CTC
Cuts at: 3895 5257 3895
Size: 1362 7033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 4127 5363 5396 6458 7055 7842
Size: 393 680 1815 1236 33 1062 597 787
Cuts at: 7842 8110 1239
Size: 268 1524
Fragments arranged by size:
1815 1524 1236 1062 787 680 597 393
268 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3126 3178 3258 3126
Size: 52 80 8263
Fragments arranged by size:
8263 80 52
EcoO109I rG'GnC_Cy
Cuts at: 2450 3015 3171 3800 3976 2450
Size: 565 156 629 176 6869
Fragments arranged by size:
6869 629 565 176 156
EcoRI G'AATT_C
Cuts at: 2292 5704 6082 2292
Size: 3412 378 4605
Fragments arranged by size:
4605 3412 378
EcoRV GAT'ATC
Cuts at: 2140 4063 2140
Size: 1923 6472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2342 2342
Size: 8395
FspI TGC'GCA
Cuts at: 6901 6901
Size: 8395
HaeII r_GCGC'y
Cuts at: 1678 7542 1678
Size: 5864 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2236 2268 4946 4978 7036
Size: 32 135 32 664 32 2678 32 2058
Cuts at: 7036 7068 7110 7142 1373
Size: 32 42 32 2626
Fragments arranged by size:
2678 2626 2058 664 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4132 5285 5739 5985 669
Size: 988 281 2194 1153 454 246 3079
Fragments arranged by size:
3079 2194 1153 988 454 281 246
HindIII A'AGCT_T
Cuts at: 748 2429 3698 748
Size: 1681 1269 5445
Fragments arranged by size:
5445 1681 1269
HpaI GTT'AAC
Cuts at: 5985 5985
Size: 8395
KpnI G_GTAC'C
Cuts at: 2033 5720 2033
Size: 3687 4708
MfeI C'AATT_G
Cuts at: 5994 5994
Size: 8395
MluI A'CGCG_T
Cuts at: 8271 8271
Size: 8395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 2184 4733 5472 7509 7693 1305
Size: 699 180 2549 739 2037 184 2007
Fragments arranged by size:
2549 2037 2007 739 699 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 10
Size: 54 1425 343 672 5901
Fragments arranged by size:
5901 1425 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2871 3609 5414 6353 6712 6871 518
Size: 648 1705 738 1805 939 359 159 2042
Fragments arranged by size:
2042 1805 1705 939 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2222 3091 3392 3736 4007 5341 5371 5811
Size: 616 869 301 344 271 1334 30 440
Cuts at: 5811 6488 7636 7881 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 869 677 616 440 344
301 271 245 30
NaeI GCC'GGC
Cuts at: 3113 3113
Size: 8395
NcoI C'CATG_G
Cuts at: 513 1065 4056 4497 4689 5502 513
Size: 552 2991 441 192 813 3406
Fragments arranged by size:
3406 2991 813 552 441 192
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8395
NgoMIV G'CCGG_C
Cuts at: 3111 3111
Size: 8395
NheI G'CTAG_C
Cuts at: 1052 4907 4915 1052
Size: 3855 8 4532
Fragments arranged by size:
4532 3855 8
NotI GC'GGCC_GC
Cuts at: 5842 5842
Size: 8395
NspI r_CATG'y
Cuts at: 1132 1229 4014 5753 7298 1132
Size: 97 2785 1739 1545 2229
Fragments arranged by size:
2785 2229 1739 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3804 1348
Size: 2456 5939
PciI A'CATG_T
Cuts at: 7294 7294
Size: 8395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4057 4498 4503 4589 1108
Size: 2949 441 5 86 4914
Fragments arranged by size:
4914 2949 441 86 5
PfoI T'CCnGG_A
Cuts at: 2568 3432 5319 2568
Size: 864 1887 5644
Fragments arranged by size:
5644 1887 864
PmeI GTTT'AAAC
Cuts at: 5699 5699
Size: 8395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4713 4745 5015 5047 6464 6496 7977
Size: 32 1310 32 270 32 1417 32 1481
Cuts at: 7977 8009 3371
Size: 32 3757
Fragments arranged by size:
3757 1481 1417 1310 270 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2450 3015 3800 2450
Size: 565 785 7045
Fragments arranged by size:
7045 785 565
PshAI GACnn'nnGTC
Cuts at: 1613 3759 5454 1613
Size: 2146 1695 4554
Fragments arranged by size:
4554 2146 1695
PsiI TTA'TAA
Cuts at: 2102 5965 2102
Size: 3863 4532
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2257 2289 2257
Size: 32 8363
PstI C_TGCA'G
Cuts at: 830 2584 3069 3708 3972 4071 4537 5747 830
Size: 1754 485 639 264 99 466 1210 3478
Fragments arranged by size:
3478 1754 1210 639 485 466 264 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8395
PvuII CAG'CTG
Cuts at: 3091 3736 4007 5341 5371 3091
Size: 645 271 1334 30 6115
Fragments arranged by size:
6115 1334 645 271 30
SacI G_AGCT'C
Cuts at: 3897 5259 3897
Size: 1362 7033
SalI G'TCGA_C
Cuts at: 1936 5737 1936
Size: 3801 4594
SanDI GG'GwC_CC
Cuts at: 2450 3800 2450
Size: 1350 7045
SbfI CC_TGCA'GG
Cuts at: 5747 5747
Size: 8395
ScaI AGT'ACT
Cuts at: 3217 6643 8355 3217
Size: 3426 1712 3257
Fragments arranged by size:
3426 3257 1712
SfcI C'TryA_G
Cuts at: 826 1045 2580 2768 3065 3086 3704 3968 4067
Size: 219 1535 188 297 21 618 264 99
Cuts at: 4067 4533 5438 5743 6878 7559 7750 826
Size: 466 905 305 1135 681 191 1471
Fragments arranged by size:
1535 1471 1135 905 681 618 466 305
297 264 219 191 188 99 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8395
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8395
SmaI CCC'GGG
Cuts at: 1872 5246 5722 1872
Size: 3374 476 4545
Fragments arranged by size:
4545 3374 476
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2432 3221 3467 3548 3994
Size: 197 926 189 300 789 246 81 446
Cuts at: 3994 4037 5123 6499 7400 7662 820
Size: 43 1086 1376 901 262 1553
Fragments arranged by size:
1553 1376 1086 926 901 789 446 300
262 246 197 189 81 43
SnaBI TAC'GTA
Cuts at: 493 6186 493
Size: 5693 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8395
SphI G_CATG'C
Cuts at: 1132 5753 1132
Size: 4621 3774
SspI AAT'ATT
Cuts at: 5 52 3237 4700 6319 5
Size: 47 3185 1463 1619 2081
Fragments arranged by size:
3185 2081 1619 1463 47
StuI AGG'CCT
Cuts at: 1517 5190 1517
Size: 3673 4722
StyI C'CwwG_G
Cuts at: 513 1065 2808 2848 4020 4056 4497 4689 4743
Size: 552 1743 40 1172 36 441 192 54
Cuts at: 4743 5502 5834 513
Size: 759 332 3074
Fragments arranged by size:
3074 1743 1172 759 552 441 332 192
54 40 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 3738 4358 5495 6422 6439 6592
Size: 1255 534 1624 620 1137 927 17 153
Cuts at: 6592 6777 7222 325
Size: 185 445 1498
Fragments arranged by size:
1624 1498 1255 1137 927 620 534 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2314 3163 3215
Size: 274 80 33 51 1443 337 849 52
Cuts at: 3215 4266 6641 8353 96
Size: 1051 2375 1712 138
Fragments arranged by size:
2375 1712 1443 1051 849 337 274 138
80 52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3767 3799 4192 4224 8355 8387 3767
Size: 32 393 32 4131 32 3775
Fragments arranged by size:
4131 3775 393 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3725 1316
Size: 2409 5986
XbaI T'CTAG_A
Cuts at: 5731 5731
Size: 8395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3457 3755 3457
Size: 298 8097
XhoI C'TCGA_G
Cuts at: 1943 3548 1943
Size: 1605 6790
XmaI C'CCGG_G
Cuts at: 1870 5244 5720 1870
Size: 3374 476 4545
Fragments arranged by size:
4545 3374 476
XmnI GAAnn'nnTTC
Cuts at: 2292 2325 2724 4728 5517 6524 2292
Size: 33 399 2004 789 1007 4163
Fragments arranged by size:
4163 2004 1007 789 399 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6203 276
Size: 53 83 186 936 4669 2468
Fragments arranged by size:
4669 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI