Restriction map for
pFN21AB3124
(Circular) (Six-base) MAPSORT of: pFN21AB3124.seq Check: 7403 from: 1 to: 6373
pFN21AB3124 6373 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2110 4183 278
Size: 53 83 186 936 574 2073 2468
Fragments arranged by size:
2468 2073 936 574 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2582 3716 1937
Size: 645 1134 4594
Fragments arranged by size:
4594 1134 645
Acc65I G'GTAC_C
Cuts at: 3297 3694 3297
Size: 397 5976
AclI AA'CG_TT
Cuts at: 1563 2446 3222 4500 4873 1563
Size: 883 776 1278 373 3063
Fragments arranged by size:
3063 1278 883 776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 3070 3082 3513 4436 5820 6088 1632
Size: 983 455 12 431 923 1384 268 1917
Fragments arranged by size:
1917 1384 983 923 455 431 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6176
AflIII A'CryG_T
Cuts at: 1203 2115 5272 6249 1203
Size: 912 3157 977 1327
Fragments arranged by size:
3157 1327 977 912
AgeI A'CCGG_T
Cuts at: 5204 5204
Size: 6373
AhdI GACnn_n'nnGTC
Cuts at: 5102 5102
Size: 6373
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 5257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 2504
Size: 32 6341
AlwNI CAG_nnn'CTG
Cuts at: 3137 4116 5688 3137
Size: 979 1572 3822
Fragments arranged by size:
3822 1572 979
ApaLI G'TGCA_C
Cuts at: 4430 5586 4430
Size: 1156 5217
ApoI r'AATT_y
Cuts at: 1493 2324 2407 3057 3197 3682 3908 4060 6169
Size: 831 83 650 140 485 226 152 2109
Cuts at: 6169 1493
Size: 1697
Fragments arranged by size:
2109 1697 831 650 485 226 152 140
83
AseI AT'TA_AT
Cuts at: 160 1032 4927 160
Size: 872 3895 1606
Fragments arranged by size:
3895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 3698 1870
Size: 73 1170 585 4545
Fragments arranged by size:
4545 1170 585 73
BamHI G'GATC_C
Cuts at: 2941 3703 4070 2941
Size: 762 367 5244
Fragments arranged by size:
5244 762 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3297 3694 5149 618
Size: 325 217 627 1510 397 1455 1842
Fragments arranged by size:
1842 1510 1455 627 397 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2503 3692 1391
Size: 688 424 1189 4072
Fragments arranged by size:
4072 1189 688 424
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2387 3625 928
Size: 971 488 1238 3676
Fragments arranged by size:
3676 1238 971 488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4564 4598 1903
Size: 34 2627 34 3678
Fragments arranged by size:
3678 2627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4265 5475 4265
Size: 1210 5163
BclI T'GATC_A
Cuts at: 1548 2167 1548
Size: 619 5754
BfrBI ATG'CAT
Cuts at: 3437 3437
Size: 6373
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4984 136
Size: 107 122 71 631 605 3312 1525
Fragments arranged by size:
3312 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2178 2425 6368 2178
Size: 247 3943 2183
Fragments arranged by size:
3943 2183 247
BlpI GC'TnA_GC
Cuts at: 2051 2677 3790 2051
Size: 626 1113 4634
Fragments arranged by size:
4634 1113 626
Bme1580I G_kGCm'C
Cuts at: 1790 3527 3613 4434 5590 1790
Size: 1737 86 821 1156 2573
Fragments arranged by size:
2573 1737 1156 821 86
BmgBI CAC'GTC
Cuts at: 1318 3454 1318
Size: 2136 4237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2533 2725 4104
Size: 416 53 83 399 24 1116 192 1379
Cuts at: 4104 5062 442
Size: 958 1753
Fragments arranged by size:
1753 1379 1116 958 416 399 192 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 2615 5033 1239
Size: 1292 84 2418 2579
Fragments arranged by size:
2579 2418 1292 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2193 2655 4498 5363 5661 2063
Size: 130 462 1843 865 298 2775
Fragments arranged by size:
2775 1843 865 462 298 130
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2713 2786 5036 882
Size: 617 1214 73 2250 2219
Fragments arranged by size:
2250 2219 1214 617 73
BsaAI yAC'GTr
Cuts at: 493 1204 2116 2455 4164 493
Size: 711 912 339 1709 2702
Fragments arranged by size:
2702 1709 912 711 339
BsaBI GATnn'nnATC
Cuts at: 2559 6367 2559
Size: 3808 2565
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2107 4180 4562 5197
Size: 53 83 186 936 574 2073 382 635
Cuts at: 5197 275
Size: 1451
Fragments arranged by size:
2073 1451 936 635 574 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4806 5204 5478 5625 6036 4806
Size: 398 274 147 411 5143
Fragments arranged by size:
5143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2504 2534 2682
Size: 30 165 30 807 30 264 30 148
Cuts at: 2682 2712 1178
Size: 30 4839
Fragments arranged by size:
4839 807 264 165 148 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 3614 1186
Size: 1048 1380 3945
Fragments arranged by size:
3945 1380 1048
BseYI C'CCAG_C
Cuts at: 3046 3560 5576 6072 3046
Size: 514 2016 496 3347
Fragments arranged by size:
3347 2016 514 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2020 1163
Size: 573 284 5516
Fragments arranged by size:
5516 573 284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3823 4584 5612 1540
Size: 275 186 1822 761 1028 2301
Fragments arranged by size:
2301 1822 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2079 3094 4434 4519 5590 2052
Size: 27 1015 1340 85 1071 2835
Fragments arranged by size:
2835 1340 1071 1015 85 27
BsmI GAATG_Cn'
Cuts at: 3439 3884 3977 3439
Size: 445 93 5835
Fragments arranged by size:
5835 445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2079 2503 3094 3527 3613 3692
Size: 399 262 27 424 591 433 86 79
Cuts at: 3692 4434 4519 5590 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 591 433 424 399 262
86 85 79 27
BspEI T'CCGG_A
Cuts at: 6036 6036
Size: 6373
BspHI T'CATG_A
Cuts at: 2823 4262 5992 2823
Size: 1439 1730 3204
Fragments arranged by size:
3204 1730 1439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 3285 3728 844
Size: 1363 1078 443 3489
Fragments arranged by size:
3489 1363 1078 443
BsrBI CCG'CTC
Cuts at: 1394 1823 2515 4260 1394
Size: 429 692 1745 3507
Fragments arranged by size:
3507 1745 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3265 4868 5042 65
Size: 1183 2017 1603 174 1396
Fragments arranged by size:
2017 1603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5017 5204 1700
Size: 110 3207 187 2869
Fragments arranged by size:
3207 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6373
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6373
BssSI C'ACGA_G
Cuts at: 2153 2640 2783 4433 5445 2153
Size: 487 143 1650 1012 3081
Fragments arranged by size:
3081 1650 1012 487 143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3225 794
Size: 2431 3942
BstBI TT'CG_AA
Cuts at: 5177 5177
Size: 6373
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6373
BstXI CCAn_nnnn'nTGG
Cuts at: 3026 3026
Size: 6373
BstYI r'GATC_y
Cuts at: 1972 2178 2425 2851 2941 3703 4070 4455 4472
Size: 206 247 426 90 762 367 385 17
Cuts at: 4472 5924 6010 6022 6368 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 762 426 385 367 346 247
206 90 86 17 12
BstZ17I GTA'TAC
Cuts at: 2583 2583
Size: 6373
BtgI C'CryG_G
Cuts at: 513 1065 2004 2712 513
Size: 552 939 708 4174
Fragments arranged by size:
4174 939 708 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5634
Fragments arranged by size:
5634 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 2770 3315 3527 3889 4683 4703 2052
Size: 718 545 212 362 794 20 3722
Fragments arranged by size:
3722 794 718 545 362 212 20
ClaI AT'CG_AT
Cuts at: 1554 2949 1554
Size: 1395 4978
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6338
DraI TTT'AAA
Cuts at: 2422 3677 2422
Size: 1255 5118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5380 809
Size: 507 4064 1802
Fragments arranged by size:
4064 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2389 2838 3820
Size: 54 1425 50 275 18 559 449 982
Cuts at: 3820 4709 6338 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 982 889 559 449 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3820 1537
Size: 275 2008 4090
Fragments arranged by size:
4090 2008 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 3411 3560 4303 1353
Size: 1082 976 149 743 3423
Fragments arranged by size:
3423 1082 976 743 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2513 4960 5334 5480 387
Size: 1064 138 924 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 924 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 2077
Size: 6373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2531 2615 3070 3082 3513 4436 5033
Size: 393 899 84 455 12 431 923 597
Cuts at: 5033 5820 6088 1239
Size: 787 268 1524
Fragments arranged by size:
1524 923 899 787 597 455 431 393
268 84 12
EcoO109I rG'GnC_Cy
Cuts at: 2915 2915
Size: 6373
EcoRI G'AATT_C
Cuts at: 2324 3682 4060 2324
Size: 1358 378 4637
Fragments arranged by size:
4637 1358 378
EcoRV GAT'ATC
Cuts at: 3469 3469
Size: 6373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2357 2357
Size: 6373
FspI TGC'GCA
Cuts at: 4879 4879
Size: 6373
HaeII r_GCGC'y
Cuts at: 1678 2058 5520 1678
Size: 380 3462 2531
Fragments arranged by size:
3462 2531 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 3376 3408 5014
Size: 32 135 32 494 32 1278 32 1606
Cuts at: 5014 5046 5088 5120 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1606 1278 494 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3361 3717 3963 669
Size: 988 281 1423 356 246 3079
Fragments arranged by size:
3079 1423 988 356 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6373
HpaI GTT'AAC
Cuts at: 3963 3963
Size: 6373
KpnI G_GTAC'C
Cuts at: 3301 3698 3301
Size: 397 5976
MfeI C'AATT_G
Cuts at: 3972 3972
Size: 6373
MluI A'CGCG_T
Cuts at: 6249 6249
Size: 6373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3254 5487 5671 1305
Size: 1949 2233 184 2007
Fragments arranged by size:
2233 2007 1949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2391 2840 10
Size: 54 1425 343 559 449 3543
Fragments arranged by size:
3543 1425 559 449 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2951 4331 4690 4849 518
Size: 648 1785 1380 359 159 2042
Fragments arranged by size:
2042 1785 1380 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2593 3050 3137 3789 4466 5614 5859 1606
Size: 987 457 87 652 677 1148 245 2120
Fragments arranged by size:
2120 1148 987 677 652 457 245 87
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4882
Fragments arranged by size:
4882 939 552
NdeI CA'TA_TG
Cuts at: 387 2386 387
Size: 1999 4374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6373
NotI GC'GGCC_GC
Cuts at: 3820 3820
Size: 6373
NsiI A_TGCA'T
Cuts at: 3439 3439
Size: 6373
NspI r_CATG'y
Cuts at: 1132 1229 2404 2983 3731 5276 1132
Size: 97 1175 579 748 1545 2229
Fragments arranged by size:
2229 1545 1175 748 579 97
PasI CC'CwG_GG
Cuts at: 1348 2188 1348
Size: 840 5533
PciI A'CATG_T
Cuts at: 5272 5272
Size: 6373
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6373
PfoI T'CCnGG_A
Cuts at: 2508 2508
Size: 6373
PmeI GTTT'AAAC
Cuts at: 3677 3677
Size: 6373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2504 2536 4442 4474 5955 5987 2504
Size: 32 1906 32 1481 32 2890
Fragments arranged by size:
2890 1906 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2915 2915
Size: 6373
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6373
PsiI TTA'TAA
Cuts at: 3943 3943
Size: 6373
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2525 2557 2525
Size: 32 6341
PstI C_TGCA'G
Cuts at: 830 3250 3725 830
Size: 2420 475 3478
Fragments arranged by size:
3478 2420 475
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6373
PvuII CAG'CTG
Cuts at: 2593 3050 3137 2593
Size: 457 87 5829
Fragments arranged by size:
5829 457 87
SacI G_AGCT'C
Cuts at: 2079 2079
Size: 6373
SalI G'TCGA_C
Cuts at: 1936 3715 1936
Size: 1779 4594
SapI GCTCTTCn'nnn_
Cuts at: 3411 3411
Size: 6373
SbfI CC_TGCA'GG
Cuts at: 3725 3725
Size: 6373
ScaI AGT'ACT
Cuts at: 4621 6333 4621
Size: 1712 4661
SfcI C'TryA_G
Cuts at: 826 1045 3246 3721 4856 5537 5728 826
Size: 219 2201 475 1135 681 191 1471
Fragments arranged by size:
2201 1471 1135 681 475 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6373
SmaI CCC'GGG
Cuts at: 1872 3700 1872
Size: 1828 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2208 2670 4477 5378 5640
Size: 197 926 135 130 462 1807 901 262
Cuts at: 5640 820
Size: 1553
Fragments arranged by size:
1807 1553 926 901 462 262 197 135
130
SnaBI TAC'GTA
Cuts at: 493 4164 493
Size: 3671 2702
SpeI A'CTAG_T
Cuts at: 152 3143 152
Size: 2991 3382
SphI G_CATG'C
Cuts at: 1132 3731 1132
Size: 2599 3774
SspI AAT'ATT
Cuts at: 5 52 4297 5
Size: 47 4245 2081
Fragments arranged by size:
4245 2081 47
StuI AGG'CCT
Cuts at: 1517 3617 1517
Size: 2100 4273
StyI C'CwwG_G
Cuts at: 513 1065 2004 2845 3812 513
Size: 552 939 841 967 3074
Fragments arranged by size:
3074 967 939 841 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2923 3168 4400 4417 4570 4755 5200
Size: 1255 1343 245 1232 17 153 185 445
Cuts at: 5200 325
Size: 1498
Fragments arranged by size:
1498 1343 1255 1232 445 245 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 4619 6331
Size: 274 80 33 51 1443 249 2393 1712
Cuts at: 6331 96
Size: 138
Fragments arranged by size:
2393 1712 1443 274 249 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2682 2714 6333 6365 2682
Size: 32 3619 32 2690
Fragments arranged by size:
3619 2690 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6373
XbaI T'CTAG_A
Cuts at: 3709 3709
Size: 6373
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6373
XmaI C'CCGG_G
Cuts at: 1870 3698 1870
Size: 1828 4545
XmnI GAAnn'nnTTC
Cuts at: 2600 2873 3014 4502 2600
Size: 273 141 1488 4471
Fragments arranged by size:
4471 1488 273 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2108 4181 276
Size: 53 83 186 936 574 2073 2468
Fragments arranged by size:
2468 2073 936 574 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI