Restriction map for
pFN21AB3118
(Circular) (Six-base) MAPSORT of: pFN21AB3118.seq Check: 169 from: 1 to: 7249
pFN21AB3118 7249 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5059 278
Size: 53 83 186 936 3523 2468
Fragments arranged by size:
3523 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4592 1937
Size: 2655 4594
Acc65I G'GTAC_C
Cuts at: 4570 4570
Size: 7249
AclI AA'CG_TT
Cuts at: 1563 5376 5749 1563
Size: 3813 373 3063
Fragments arranged by size:
3813 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 2804 2958 3703 3777 4469 5312 6696
Size: 477 695 154 745 74 692 843 1384
Cuts at: 6696 6964 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 843 745 695 692 477 268
154 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7052
AflIII A'CryG_T
Cuts at: 1203 4292 6148 7125 1203
Size: 3089 1856 977 1327
Fragments arranged by size:
3089 1856 1327 977
AgeI A'CCGG_T
Cuts at: 6080 6080
Size: 7249
AhdI GACnn_n'nnGTC
Cuts at: 5978 5978
Size: 7249
AleI CACnn'nnGTG
Cuts at: 1166 4349 1166
Size: 3183 4066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2965 1184
Size: 1781 5468
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4091 4123 4091
Size: 32 7217
AlwNI CAG_nnn'CTG
Cuts at: 2368 2561 4992 6564 2368
Size: 193 2431 1572 3053
Fragments arranged by size:
3053 2431 1572 193
ApaLI G'TGCA_C
Cuts at: 2950 5306 6462 2950
Size: 2356 1156 3737
Fragments arranged by size:
3737 2356 1156
ApoI r'AATT_y
Cuts at: 1493 2317 2351 2510 2716 2854 3683 4558 4784
Size: 824 34 159 206 138 829 875 226
Cuts at: 4784 4936 7045 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 875 829 824 226 206 159
152 138 34
AseI AT'TA_AT
Cuts at: 160 1032 2187 5803 160
Size: 872 1155 3616 1606
Fragments arranged by size:
3616 1606 1155 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
AvaI C'yCGr_G
Cuts at: 1870 1943 4574 1870
Size: 73 2631 4545
Fragments arranged by size:
4545 2631 73
AvrII C'CTAG_G
Cuts at: 3640 3640
Size: 7249
BamHI G'GATC_C
Cuts at: 3277 4579 4946 3277
Size: 1302 367 5580
Fragments arranged by size:
5580 1302 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4570 6025 618
Size: 325 217 627 2783 1455 1842
Fragments arranged by size:
2783 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3594 4568 1391
Size: 2203 974 4072
Fragments arranged by size:
4072 2203 974
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 3133 3663 928
Size: 971 606 628 530 4514
Fragments arranged by size:
4514 971 628 606 530
BbvCI CC'TCA_GC
Cuts at: 4338 4338
Size: 7249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5440 5474 1903
Size: 34 3503 34 3678
Fragments arranged by size:
3678 3503 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5141 6351 5141
Size: 1210 6039
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7249
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5860 136
Size: 107 122 71 631 605 4188 1525
Fragments arranged by size:
4188 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7244 7244
Size: 7249
BlpI GC'TnA_GC
Cuts at: 2219 4666 2219
Size: 2447 4802
Bme1580I G_kGCm'C
Cuts at: 1790 2954 5310 6466 1790
Size: 1164 2356 1156 2573
Fragments arranged by size:
2573 2356 1164 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7249
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 4980 5938
Size: 416 53 83 399 24 1122 2441 958
Cuts at: 5938 442
Size: 1753
Fragments arranged by size:
2441 1753 1122 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2160 1056
Size: 1104 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2616 3054 3249 3597 5909 1239
Size: 1377 438 195 348 2312 2579
Fragments arranged by size:
2579 2312 1377 438 348 195
Bpu10I CC'TnA_GC
Cuts at: 2962 4338 2962
Size: 1376 5873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 5374 6239 6537 3814
Size: 1560 865 298 4526
Fragments arranged by size:
4526 1560 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2118 2594 3148 5912 882
Size: 617 619 476 554 2764 2219
Fragments arranged by size:
2764 2219 619 617 554 476
BsaAI yAC'GTr
Cuts at: 493 1204 4351 5040 493
Size: 711 3147 689 2702
Fragments arranged by size:
3147 2702 711 689
BsaBI GATnn'nnATC
Cuts at: 7243 7243
Size: 7249
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5056 5438 6073 275
Size: 53 83 186 936 3523 382 635 1451
Fragments arranged by size:
3523 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2844 5682 6080 6354 6501 6912 2844
Size: 2838 398 274 147 411 3181
Fragments arranged by size:
3181 2838 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3222 3252 1178
Size: 30 165 30 1819 30 5175
Fragments arranged by size:
5175 1819 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4275 1186
Size: 3089 4160
BseYI C'CCAG_C
Cuts at: 2083 4301 6452 6948 2083
Size: 2218 2151 496 2384
Fragments arranged by size:
2384 2218 2151 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 3423 3489 1163
Size: 573 345 1342 66 4923
Fragments arranged by size:
4923 1342 573 345 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4699 5460 6488 1540
Size: 275 186 2698 761 1028 2301
Fragments arranged by size:
2698 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2954 5310 5395 6466 2954
Size: 2356 85 1071 3737
Fragments arranged by size:
3737 2356 1071 85
BsmI GAATG_Cn'
Cuts at: 3485 4760 4853 3485
Size: 1275 93 5881
Fragments arranged by size:
5881 1275 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2954 3594 4568 5310 5395 6466 1391
Size: 399 1164 640 974 742 85 1071 2174
Fragments arranged by size:
2174 1164 1071 974 742 640 399 85
BspEI T'CCGG_A
Cuts at: 2844 6912 2844
Size: 4068 3181
BspHI T'CATG_A
Cuts at: 5138 6868 5138
Size: 1730 5519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4604 844
Size: 3760 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5136 1394
Size: 429 3313 3507
Fragments arranged by size:
3507 3313 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2718 5744 5918 65
Size: 1183 1470 3026 174 1396
Fragments arranged by size:
3026 1470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 5893 6080 1700
Size: 110 246 3837 187 2869
Fragments arranged by size:
3837 2869 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 2552 3621 4145 96
Size: 2456 1069 524 3200
Fragments arranged by size:
3200 2456 1069 524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7249
BssSI C'ACGA_G
Cuts at: 2953 5309 6321 2953
Size: 2356 1012 3881
Fragments arranged by size:
3881 2356 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7249
BstBI TT'CG_AA
Cuts at: 6053 6053
Size: 7249
BstEII G'GTnAC_C
Cuts at: 1184 3581 1184
Size: 2397 4852
BstXI CCAn_nnnn'nTGG
Cuts at: 4253 4253
Size: 7249
BstYI r'GATC_y
Cuts at: 1972 3277 4579 4946 5331 5348 6800 6886 6898
Size: 1305 1302 367 385 17 1452 86 12
Cuts at: 6898 7244 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1305 1302 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3555 3555
Size: 7249
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6510
Fragments arranged by size:
6510 683 56
BtsI GCAGTG_nn'
Cuts at: 4765 5559 5579 4765
Size: 794 20 6435
Fragments arranged by size:
6435 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2772 2807 529
Size: 35 2208 35 4971
Fragments arranged by size:
4971 2208 35 35
DraI TTT'AAA
Cuts at: 4401 4425 4553 4401
Size: 24 128 7097
Fragments arranged by size:
7097 128 24
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6256 809
Size: 507 4940 1802
Fragments arranged by size:
4940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4696 5585 7214
Size: 54 1425 50 275 18 2866 889 1629
Cuts at: 7214 8
Size: 43
Fragments arranged by size:
2866 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4696 1537
Size: 275 2884 4090
Fragments arranged by size:
4090 2884 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5179 1353
Size: 3826 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 5836 6210 6356 387
Size: 1064 138 476 3771 374 146 1280
Fragments arranged by size:
3771 1280 1064 476 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2616 2804 2958 3054 3249 3597
Size: 393 477 507 188 154 96 195 348
Cuts at: 3597 3703 3777 4469 5312 5909 6696 6964 1239
Size: 106 74 692 843 597 787 268 1524
Fragments arranged by size:
1524 843 787 692 597 507 477 393
348 268 195 188 154 106 96 74
EcoRI G'AATT_C
Cuts at: 2351 4558 4936 2351
Size: 2207 378 4664
Fragments arranged by size:
4664 2207 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 3799 2266
Size: 1533 5716
FspI TGC'GCA
Cuts at: 5755 5755
Size: 7249
HaeII r_GCGC'y
Cuts at: 1678 2022 6396 1678
Size: 344 4374 2531
Fragments arranged by size:
4374 2531 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2681 2713 2797 2829 3551
Size: 32 135 32 1109 32 84 32 722
Cuts at: 3551 3583 4517 4549 5890 5922 5964 5996 1373
Size: 32 934 32 1341 32 42 32 2626
Fragments arranged by size:
2626 1341 1109 934 722 135 84 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3715 4593 4839 669
Size: 988 281 1777 878 246 3079
Fragments arranged by size:
3079 1777 988 878 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7249
HpaI GTT'AAC
Cuts at: 4839 4839
Size: 7249
KpnI G_GTAC'C
Cuts at: 4574 4574
Size: 7249
MfeI C'AATT_G
Cuts at: 3991 4848 3991
Size: 857 6392
MluI A'CGCG_T
Cuts at: 7125 7125
Size: 7249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 2175 6363 6547 1305
Size: 819 51 4188 184 2007
Fragments arranged by size:
4188 2007 819 184 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5427
Fragments arranged by size:
5427 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 4349 5207 5566 5725 518
Size: 648 1306 1877 858 359 159 2042
Fragments arranged by size:
2042 1877 1306 858 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 2987 3916 4442 4665 5342 6490 6735
Size: 481 900 929 526 223 677 1148 245
Cuts at: 6735 1606
Size: 2120
Fragments arranged by size:
2120 1148 929 900 677 526 481 245
223
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6697
NdeI CA'TA_TG
Cuts at: 387 2666 387
Size: 2279 4970
NheI G'CTAG_C
Cuts at: 1052 2156 1052
Size: 1104 6145
NotI GC'GGCC_GC
Cuts at: 4696 4696
Size: 7249
NspI r_CATG'y
Cuts at: 1132 1229 3673 3940 3955 4296 4607 6152 1132
Size: 97 2444 267 15 341 311 1545 2229
Fragments arranged by size:
2444 2229 1545 341 311 267 97 15
PasI CC'CwG_GG
Cuts at: 1348 3587 1348
Size: 2239 5010
PciI A'CATG_T
Cuts at: 4292 6148 4292
Size: 1856 5393
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7249
PmeI GTTT'AAAC
Cuts at: 4553 4553
Size: 7249
PmlI CAC'GTG
Cuts at: 4351 4351
Size: 7249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5318 5350 6831 6863 5318
Size: 32 1481 32 5704
Fragments arranged by size:
5704 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2603 3089 3755 1613
Size: 990 486 666 5107
Fragments arranged by size:
5107 990 666 486
PsiI TTA'TAA
Cuts at: 2613 3117 4819 2613
Size: 504 1702 5043
Fragments arranged by size:
5043 1702 504
PstI C_TGCA'G
Cuts at: 830 4601 830
Size: 3771 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7249
PvuII CAG'CTG
Cuts at: 2087 2987 3916 4442 2087
Size: 900 929 526 4894
Fragments arranged by size:
4894 929 900 526
SalI G'TCGA_C
Cuts at: 1936 4591 1936
Size: 2655 4594
SbfI CC_TGCA'GG
Cuts at: 4601 4601
Size: 7249
ScaI AGT'ACT
Cuts at: 3427 3436 4377 5497 7209 3427
Size: 9 941 1120 1712 3467
Fragments arranged by size:
3467 1712 1120 941 9
SfcI C'TryA_G
Cuts at: 826 1045 2305 2394 4597 5732 6413 6604 826
Size: 219 1260 89 2203 1135 681 191 1471
Fragments arranged by size:
2203 1471 1260 1135 681 219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7249
SmaI CCC'GGG
Cuts at: 1872 4576 1872
Size: 2704 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3829 5353 6254 6516 820
Size: 197 926 1886 1524 901 262 1553
Fragments arranged by size:
1886 1553 1524 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5040 493
Size: 4547 2702
SpeI A'CTAG_T
Cuts at: 152 3308 152
Size: 3156 4093
SphI G_CATG'C
Cuts at: 1132 4607 1132
Size: 3475 3774
SspI AAT'ATT
Cuts at: 5 52 5173 5
Size: 47 5121 2081
Fragments arranged by size:
5121 2081 47
StuI AGG'CCT
Cuts at: 1517 2331 1517
Size: 814 6435
StyI C'CwwG_G
Cuts at: 513 1065 2722 3640 3816 4688 513
Size: 552 1657 918 176 872 3074
Fragments arranged by size:
3074 1657 918 872 552 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2142 2517 3600 4361 5276 5293 5446
Size: 1255 562 375 1083 761 915 17 153
Cuts at: 5446 5631 6076 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1083 915 761 562 445 375
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2552 3425 3434
Size: 274 80 33 51 1443 575 873 9
Cuts at: 3434 3621 4145 4365 4375 5495 7207 96
Size: 187 524 220 10 1120 1712 138
Fragments arranged by size:
1712 1443 1120 873 575 524 274 220
187 138 80 51 33 10 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3222 3254 7209 7241 3222
Size: 32 3955 32 3230
Fragments arranged by size:
3955 3230 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7249
XbaI T'CTAG_A
Cuts at: 3859 4585 3859
Size: 726 6523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4543 4543
Size: 7249
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7249
XmaI C'CCGG_G
Cuts at: 1870 4574 1870
Size: 2704 4545
XmnI GAAnn'nnTTC
Cuts at: 5378 5378
Size: 7249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5057 276
Size: 53 83 186 936 3523 2468
Fragments arranged by size:
3523 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI