Restriction map for
pFN21AB3115
(Circular) (Six-base) MAPSORT of: pFN21AB3115.seq Check: 9191 from: 1 to: 5974
pFN21AB3115 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3167 4037 5421 5689 1632
Size: 977 558 870 1384 268 1917
Fragments arranged by size:
1917 1384 977 870 558 268
AflII C'TTAA_G
Cuts at: 820 1017 2375 820
Size: 197 1358 4419
Fragments arranged by size:
4419 1358 197
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2356 3717 5289 2356
Size: 1361 1572 3041
Fragments arranged by size:
3041 1572 1361
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2141 3299 1870
Size: 73 198 1158 4545
Fragments arranged by size:
4545 1158 198 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2149 2608 3233 3293 1391
Size: 758 459 625 60 4072
Fragments arranged by size:
4072 758 625 459 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2085 3133 1548
Size: 537 1048 4389
Fragments arranged by size:
4389 1048 537
BfrBI ATG'CAT
Cuts at: 2780 2780
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 2494 1318
Size: 1176 4798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2205 2592 3705
Size: 416 53 83 399 24 788 387 1113
Cuts at: 3705 4663 442
Size: 958 1753
Fragments arranged by size:
1753 1113 958 788 416 399 387 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2459 1056
Size: 1403 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 4634 1239
Size: 800 2595 2579
Fragments arranged by size:
2595 2579 800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2040 4099 4964 5262 2040
Size: 2059 865 298 2752
Fragments arranged by size:
2752 2059 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 2507 5968 2507
Size: 3461 2513
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2175 3781 4163
Size: 53 83 186 936 476 166 1606 382
Cuts at: 4163 4798 275
Size: 635 1451
Fragments arranged by size:
1606 1451 936 635 476 382 186 166
83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5974
BseYI C'CCAG_C
Cuts at: 2306 3112 5177 5673 2306
Size: 806 2065 496 2607
Fragments arranged by size:
2607 2065 806 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3002 3005 1163
Size: 573 1266 3 4132
Fragments arranged by size:
4132 1266 573 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2280 2608 4035 4120 5191 2142
Size: 138 328 1427 85 1071 2925
Fragments arranged by size:
2925 1427 1071 328 138 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2149 2280 2608 3233 3293 4035
Size: 399 352 7 131 328 625 60 742
Cuts at: 4035 4120 5191 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 399 352 328 131
85 60 7
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3169 3861 1394
Size: 429 1346 692 3507
Fragments arranged by size:
3507 1346 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2782 2900 4469 4643 65
Size: 1183 1534 118 1569 174 1396
Fragments arranged by size:
1569 1534 1396 1183 174 118
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 2155 3214 96
Size: 2059 1059 2856
Fragments arranged by size:
2856 2059 1059
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 2786 4034 5046 2786
Size: 1248 1012 3714
Fragments arranged by size:
3714 1248 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 2226 4778 2226
Size: 2552 3422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969
Size: 1332 367 385 17 1452 86 12 346
Cuts at: 5969 1972
Size: 1977
Fragments arranged by size:
1977 1452 1332 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2997 2997
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 2004 2643 3180 513
Size: 552 939 639 537 3307
Fragments arranged by size:
3307 939 639 552 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 3490 4284 4304 3490
Size: 794 20 5160
Fragments arranged by size:
5160 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2077 2112 529
Size: 35 1513 35 4391
Fragments arranged by size:
4391 1513 35 35
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3074 4981 809
Size: 507 1758 1907 1802
Fragments arranged by size:
1907 1802 1758 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 3904 1353
Size: 905 1646 3423
Fragments arranged by size:
3423 1646 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2606 2606
Size: 5974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2609 3167 4037 4634 5421 5689
Size: 393 407 570 558 870 597 787 268
Cuts at: 5689 1239
Size: 1524
Fragments arranged by size:
1524 870 787 597 570 558 407 393
268
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
EcoRV GAT'ATC
Cuts at: 2509 2509
Size: 5974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2559 2591 4615 4647 4689
Size: 32 135 32 987 32 2024 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 2024 987 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2065 2646 5088 5272 1305
Size: 760 581 2442 184 2007
Fragments arranged by size:
2442 2007 760 581 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 4291 4450 518
Size: 648 2766 359 159 2042
Fragments arranged by size:
2766 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2416 3390 4067 5215 5460 1606
Size: 810 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 810 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2643 3180 513
Size: 552 939 639 537 3307
Fragments arranged by size:
3307 939 639 552 537
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 2455 1052
Size: 1403 4571
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NruI TCG'CGA
Cuts at: 2868 2868
Size: 5974
NsiI A_TGCA'T
Cuts at: 2782 2782
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2432 3332 4877 1132
Size: 97 1203 900 1545 2229
Fragments arranged by size:
2229 1545 1203 900 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 2913 3544 2913
Size: 631 5343
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2416 2416
Size: 5974
SacI G_AGCT'C
Cuts at: 2608 2608
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 2258 2258
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 2835 2835
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 3322 4457 5138 5329 826
Size: 219 2277 1135 681 191 1471
Fragments arranged by size:
2277 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2055 2375 4078 4979 5241 820
Size: 197 926 112 320 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 320 262 197 112
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 2923 3898 5
Size: 47 2871 975 2081
Fragments arranged by size:
2871 2081 975 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2643 3180 3413 513
Size: 552 939 165 474 537 233 3074
Fragments arranged by size:
3074 939 552 537 474 233 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4001 4018 4171 4356 4801 325
Size: 1255 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 2716 3214
Size: 274 80 33 51 1443 178 561 498
Cuts at: 3214 4220 5932 96
Size: 1006 1712 138
Fragments arranged by size:
1712 1443 1006 561 498 274 178 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI