Restriction map for
pFN21AB3110
(Circular) (Six-base) MAPSORT of: pFN21AB3110.seq Check: 9785 from: 1 to: 5596
pFN21AB3110 5596 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2112 2624 2917 2112
Size: 512 293 4791
Fragments arranged by size:
4791 512 293
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3659 5043 5311 1632
Size: 2027 1384 268 1917
Fragments arranged by size:
2027 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 2744 4495 5472 1203
Size: 1541 1751 977 1327
Fragments arranged by size:
1751 1541 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 2510
Size: 32 5564
AlwNI CAG_nnn'CTG
Cuts at: 2089 2098 3339 4911 2089
Size: 9 1241 1572 2774
Fragments arranged by size:
2774 1572 1241 9
ApaI G_GGCC'C
Cuts at: 2071 2071
Size: 5596
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 2234 2553 2585 2592 2858 2921
Size: 73 224 67 319 32 7 266 63
Cuts at: 2921 1870
Size: 4545
Fragments arranged by size:
4545 319 266 224 73 67 63 32
7
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2171 2576 2624 2917
Size: 325 217 627 325 59 405 48 293
Cuts at: 2917 4372 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 405 325 325 293 217
59 48
BanII G_rGCy'C
Cuts at: 1391 2071 2586 2592 2681 2915 1391
Size: 680 515 6 89 234 4072
Fragments arranged by size:
4072 680 515 234 89 6
BbeI G_GCGC'C
Cuts at: 2175 2175
Size: 5596
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2894 928
Size: 971 995 3630
Fragments arranged by size:
3630 995 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2271 4207 136
Size: 107 122 71 631 605 599 1936 1525
Fragments arranged by size:
1936 1525 631 605 599 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 2429 2472 3013 2429
Size: 43 541 5012
Fragments arranged by size:
5012 541 43
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2814 3657 4813 1790
Size: 281 743 843 1156 2573
Fragments arranged by size:
2573 1156 843 743 281
BmgBI CAC'GTC
Cuts at: 1318 2293 2809 1318
Size: 975 516 4105
Fragments arranged by size:
4105 975 516
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2690 4256 1239
Size: 1451 1566 2579
Fragments arranged by size:
2579 1566 1451
Bpu10I CC'TnA_GC
Cuts at: 2654 2654
Size: 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2745 2773 3387 493
Size: 711 1541 28 614 2702
Fragments arranged by size:
2702 1541 711 614 28
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2172 2307 2757
Size: 53 83 186 936 570 69 135 450
Cuts at: 2757 3403 3785 4420 275
Size: 646 382 635 1451
Fragments arranged by size:
1451 936 646 635 570 450 382 186
135 83 69 53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2810 2840 1178
Size: 30 165 30 1407 30 3934
Fragments arranged by size:
3934 1407 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2699 1186
Size: 982 531 4083
Fragments arranged by size:
4083 982 531
BseYI C'CCAG_C
Cuts at: 2078 2125 4799 5295 2078
Size: 47 2674 496 2379
Fragments arranged by size:
2674 2379 496 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 1163
Size: 573 482 4541
Fragments arranged by size:
4541 573 482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2206 3046 3807 4835 1540
Size: 275 186 205 840 761 1028 2301
Fragments arranged by size:
2301 1028 840 761 275 205 186
BsiHKAI G_wGCw'C
Cuts at: 3657 3742 4813 3657
Size: 85 1071 4440
Fragments arranged by size:
4440 1071 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
BsmBI CGTCTCn'nnnn_
Cuts at: 2589 2589
Size: 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2586 2592 2681 2814 2915 3657
Size: 399 281 515 6 89 133 101 742
Cuts at: 3657 3742 4813 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 399 281 133 101
89 85 6
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2458 2556 2951 844
Size: 1280 334 98 395 3489
Fragments arranged by size:
3489 1280 395 334 98
BsrBI CCG'CTC
Cuts at: 1394 1823 2428 3483 1394
Size: 429 605 1055 3507
Fragments arranged by size:
3507 1055 605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2051 4091 4265 65
Size: 1183 803 2040 174 1396
Fragments arranged by size:
2040 1396 1183 803 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 2028 2074 2848 1925
Size: 103 46 774 4673
Fragments arranged by size:
4673 774 103 46
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 794
Size: 1295 4301
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2688 2926 3293 3678 3695 5147 5233 5245
Size: 716 238 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 716 385 367 346 238 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2682 2682
Size: 5596
BtgI C'CryG_G
Cuts at: 513 1065 2004 2572 513
Size: 552 939 568 3537
Fragments arranged by size:
3537 939 568 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2381 3112 3906 3926 2381
Size: 731 794 20 4051
Fragments arranged by size:
4051 794 731 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DraIII CAC_nnn'GTG
Cuts at: 2296 2377 2296
Size: 81 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2365 2611
Size: 54 1425 50 275 18 453 82 246
Cuts at: 2611 2739 3043 3932 5561 8
Size: 128 304 889 1629 43
Fragments arranged by size:
1629 1425 889 453 304 275 246 128
82 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 3526 1353
Size: 1316 857 3423
Fragments arranged by size:
3423 1316 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 2320 2390 2681 4183 4557
Size: 1064 138 480 251 70 291 1502 374
Cuts at: 4557 4703 387
Size: 146 1280
Fragments arranged by size:
1502 1280 1064 480 374 291 251 146
138 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2690 3659 4256 5043 5311 1239
Size: 393 1058 969 597 787 268 1524
Fragments arranged by size:
1524 1058 969 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2657 2657
Size: 5596
EcoO109I rG'GnC_Cy
Cuts at: 2068 2320 2784 2068
Size: 252 464 4880
Fragments arranged by size:
4880 464 252
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2063 2161 2175 2536 4743 1678
Size: 385 98 14 361 2207 2531
Fragments arranged by size:
2531 2207 385 361 98 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2680 2712 2855
Size: 32 135 32 623 32 453 32 143
Cuts at: 2855 2887 4237 4269 4311 4343 1373
Size: 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 623 453 143 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KasI G'GCGC_C
Cuts at: 2171 2171
Size: 5596
KpnI G_GTAC'C
Cuts at: 2116 2628 2921 2116
Size: 512 293 4791
Fragments arranged by size:
4791 512 293
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2613 2741 10
Size: 54 1425 343 781 128 2865
Fragments arranged by size:
2865 1425 781 343 128 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3554 3913 4072 518
Size: 648 2388 359 159 2042
Fragments arranged by size:
2388 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2574 3012 3689 4837 5082 1606
Size: 489 479 438 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 479 438 245
NarI GG'CG_CC
Cuts at: 2172 2172
Size: 5596
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4105
Fragments arranged by size:
4105 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2230 2848 2954 4499 1132
Size: 97 1001 618 106 1545 2229
Fragments arranged by size:
2229 1545 1001 618 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2545 1108
Size: 1437 4159
PfoI T'CCnGG_A
Cuts at: 2703 2703
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PmlI CAC'GTG
Cuts at: 2745 2773 2745
Size: 28 5568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PspOMI G'GGCC_C
Cuts at: 2067 2067
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2094 2948 830
Size: 1264 854 3478
Fragments arranged by size:
3478 1264 854
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2095 2095
Size: 5596
SacII CC_GC'GG
Cuts at: 2575 2575
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2669 2669
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2090 2944 4079 4760 4951 826
Size: 219 1045 854 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 854 681 219 191
SfoI GGC'GCC
Cuts at: 2173 2173
Size: 5596
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2169 2236 2923 1872
Size: 297 67 687 4545
Fragments arranged by size:
4545 687 297 67
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2004 3035 513
Size: 552 939 1031 3074
Fragments arranged by size:
3074 1031 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2303 2821 3623 3640 3793 3978
Size: 1255 458 265 518 802 17 153 185
Cuts at: 3978 4423 325
Size: 445 1498
Fragments arranged by size:
1498 1255 802 518 458 445 265 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2167 2234 2921 1870
Size: 297 67 687 4545
Fragments arranged by size:
4545 687 297 67
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI