Restriction map for
pFN21AB3109
(Circular) (Six-base) MAPSORT of: pFN21AB3109.seq Check: 521 from: 1 to: 10063
pFN21AB3109 10063 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5351 5351
Size: 10063
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 7873 278
Size: 53 83 186 936 2083 4254 2468
Fragments arranged by size:
4254 2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3526 7406 1937
Size: 1589 3880 4594
Fragments arranged by size:
4594 3880 1589
Acc65I G'GTAC_C
Cuts at: 7384 7384
Size: 10063
AclI AA'CG_TT
Cuts at: 1563 8190 8563 1563
Size: 6627 373 3063
Fragments arranged by size:
6627 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2628 2997 6393 6423 6462 6671 7152 8126
Size: 996 369 3396 30 39 209 481 974
Cuts at: 8126 9510 9778 1632
Size: 1384 268 1917
Fragments arranged by size:
3396 1917 1384 996 974 481 369 268
209 39 30
AflII C'TTAA_G
Cuts at: 820 1017 2327 3502 6977 820
Size: 197 1310 1175 3475 3906
Fragments arranged by size:
3906 3475 1310 1175 197
AflIII A'CryG_T
Cuts at: 1203 3200 6933 8962 9939 1203
Size: 1997 3733 2029 977 1327
Fragments arranged by size:
3733 2029 1997 1327 977
AgeI A'CCGG_T
Cuts at: 8894 8894
Size: 10063
AhdI GACnn_n'nnGTC
Cuts at: 2433 8792 2433
Size: 6359 3704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6714 1184
Size: 5530 4533
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 6911 6943 2063
Size: 32 4816 32 5183
Fragments arranged by size:
5183 4816 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3484 4194 4643 4800 6182 6880 6914 7806 9378
Size: 710 449 157 1382 698 34 892 1572
Cuts at: 9378 3484
Size: 4169
Fragments arranged by size:
4169 1572 1382 892 710 698 449 157
34
ApaLI G'TGCA_C
Cuts at: 4422 5497 8120 9276 4422
Size: 1075 2623 1156 5209
Fragments arranged by size:
5209 2623 1156 1075
ApoI r'AATT_y
Cuts at: 1493 2393 2680 3231 5924 7111 7372 7598 7750
Size: 900 287 551 2693 1187 261 226 152
Cuts at: 7750 9859 1493
Size: 2109 1697
Fragments arranged by size:
2693 2109 1697 1187 900 551 287 261
226 152
AseI AT'TA_AT
Cuts at: 160 1032 8617 160
Size: 872 7585 1606
Fragments arranged by size:
7585 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10063
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2075 5512 7388 1870
Size: 73 112 20 3437 1876 4545
Fragments arranged by size:
4545 3437 1876 112 73 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4204 4237 4843 4876 5041 5074 4204
Size: 33 606 33 165 33 9193
Fragments arranged by size:
9193 606 165 33 33 33
BamHI G'GATC_C
Cuts at: 7393 7760 7393
Size: 367 9696
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5105 5951 7384 8839 618
Size: 325 217 627 3318 846 1433 1455 1842
Fragments arranged by size:
3318 1842 1455 1433 846 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2056 4094 5717 7382 1391
Size: 665 2038 1623 1665 4072
Fragments arranged by size:
4072 2038 1665 1623 665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5165 5512 6081 6450 6464 6864 7073
Size: 971 3266 347 569 369 14 400 209
Cuts at: 7073 7254 928
Size: 181 3737
Fragments arranged by size:
3737 3266 971 569 400 369 347 209
181 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2796 2830 4541 4575 5060 5094 8254
Size: 34 859 34 1711 34 485 34 3160
Cuts at: 8254 8288 1903
Size: 34 3678
Fragments arranged by size:
3678 3160 1711 859 485 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2667 4844 4880 5042 7955 9165 2667
Size: 2177 36 162 2913 1210 3565
Fragments arranged by size:
3565 2913 2177 1210 162 36
BclI T'GATC_A
Cuts at: 1548 5568 1548
Size: 4020 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8674 136
Size: 107 122 71 631 605 7002 1525
Fragments arranged by size:
7002 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 10058 10058
Size: 10063
BlpI GC'TnA_GC
Cuts at: 7480 7480
Size: 10063
Bme1580I G_kGCm'C
Cuts at: 1790 2848 4426 4578 5108 5501 6367 8124 9280
Size: 1058 1578 152 530 393 866 1757 1156
Cuts at: 9280 1790
Size: 2573
Fragments arranged by size:
2573 1757 1578 1156 1058 866 530 393
152
BmgBI CAC'GTC
Cuts at: 1318 2169 4084 5383 6079 1318
Size: 851 1915 1299 696 5302
Fragments arranged by size:
5302 1915 1299 851 696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3505 3883 4443
Size: 416 53 83 399 24 2088 378 560
Cuts at: 4443 4588 4631 4653 4995 6094 7794 8752 442
Size: 145 43 22 342 1099 1700 958 1753
Fragments arranged by size:
2088 1753 1700 1099 958 560 416 399
378 342 145 83 53 43 24 22
BmtI G_CTAG'C
Cuts at: 1056 2027 1056
Size: 971 9092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2000 3426 3777 5627 8723 1239
Size: 761 1426 351 1850 3096 2579
Fragments arranged by size:
3096 2579 1850 1426 761 351
Bpu10I CC'TnA_GC
Cuts at: 2067 2067
Size: 10063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2722 3970 4982 5784 6011 6654 7182 7263 8188
Size: 1248 1012 802 227 643 528 81 925
Cuts at: 8188 9053 9351 2722
Size: 865 298 3434
Fragments arranged by size:
3434 1248 1012 925 865 802 643 528
298 227 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 2962 3269 5367 6403 8726 882
Size: 617 759 704 307 2098 1036 2323 2219
Fragments arranged by size:
2323 2219 2098 1036 759 704 617 307
BsaAI yAC'GTr
Cuts at: 493 1204 3201 6647 7854 493
Size: 711 1997 3446 1207 2702
Fragments arranged by size:
3446 2702 1997 1207 711
BsaBI GATnn'nnATC
Cuts at: 2256 2762 10057 2256
Size: 506 7295 2262
Fragments arranged by size:
7295 2262 506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3616 4783 5426 7870
Size: 53 83 186 936 2083 1167 643 2444
Cuts at: 7870 8252 8887 275
Size: 382 635 1451
Fragments arranged by size:
2444 2083 1451 1167 936 643 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3738 8496 8894 9168 9315 9726 3738
Size: 4758 398 274 147 411 4075
Fragments arranged by size:
4758 4075 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2299 2329 4161 4191 1178
Size: 30 165 30 896 30 1832 30 7050
Fragments arranged by size:
7050 1832 896 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4060 6014 6134 6512 1186
Size: 2874 1954 120 378 4737
Fragments arranged by size:
4737 2874 1954 378 120
BseYI C'CCAG_C
Cuts at: 3208 6939 9266 9762 3208
Size: 3731 2327 496 3509
Fragments arranged by size:
3731 3509 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2068 2162 2923 3917 5515 7071 1163
Size: 573 332 94 761 994 1598 1556 4155
Fragments arranged by size:
4155 1598 1556 994 761 573 332 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 2462 4010 7513 8274 9302
Size: 275 186 139 322 1548 3503 761 1028
Cuts at: 9302 1540
Size: 2301
Fragments arranged by size:
3503 2301 1548 1028 761 322 275 186
139
BsiHKAI G_wGCw'C
Cuts at: 2146 3495 3593 4426 5121 5501 8124 8209 9280
Size: 1349 98 833 695 380 2623 85 1071
Cuts at: 9280 2146
Size: 2929
Fragments arranged by size:
2929 2623 1349 1071 833 695 380 98
85
BsmI GAATG_Cn'
Cuts at: 4331 6016 7574 7667 4331
Size: 1685 1558 93 6727
Fragments arranged by size:
6727 1685 1558 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5417 5417
Size: 10063
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2146 2848 3495 3593 4094 4426
Size: 399 266 90 702 647 98 501 332
Cuts at: 4426 4578 5108 5121 5501 5717 6367 7382 8124
Size: 152 530 13 380 216 650 1015 742
Cuts at: 8124 8209 9280 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1015 742 702 650 647 530
501 399 380 332 266 216 152 98
90 85 13
BspEI T'CCGG_A
Cuts at: 9726 9726
Size: 10063
BspHI T'CATG_A
Cuts at: 2260 6057 7952 9682 2260
Size: 3797 1895 1730 2641
Fragments arranged by size:
3797 2641 1895 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2980 4418 5351 6888 7418 844
Size: 2136 1438 933 1537 530 3489
Fragments arranged by size:
3489 2136 1537 1438 933 530
BsrBI CCG'CTC
Cuts at: 1394 1823 3721 7950 1394
Size: 429 1898 4229 3507
Fragments arranged by size:
4229 3507 1898 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3022 3267 3514 4618 5269 5299 5571
Size: 1183 1774 245 247 1104 651 30 272
Cuts at: 5571 6499 6705 8558 8732 65
Size: 928 206 1853 174 1396
Fragments arranged by size:
1853 1774 1396 1183 1104 928 651 272
247 245 206 174 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 3597 3761 4009 4386 5040 8707 8894
Size: 110 1787 164 248 377 654 3667 187
Cuts at: 8894 1700
Size: 2869
Fragments arranged by size:
3667 2869 1787 654 377 248 187 164
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10063
BssHII G'CGCG_C
Cuts at: 1925 2062 1925
Size: 137 9926
BssSI C'ACGA_G
Cuts at: 8123 9135 8123
Size: 1012 9051
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5352 5538 794
Size: 4558 186 5319
Fragments arranged by size:
5319 4558 186
BstBI TT'CG_AA
Cuts at: 8867 8867
Size: 10063
BstEII G'GTnAC_C
Cuts at: 1184 2158 4180 1184
Size: 974 2022 7067
Fragments arranged by size:
7067 2022 974
BstXI CCAn_nnnn'nTGG
Cuts at: 3495 4613 5857 6343 3495
Size: 1118 1244 486 7215
Fragments arranged by size:
7215 1244 1118 486
BstYI r'GATC_y
Cuts at: 1972 2251 2635 2656 3175 3355 4484 4810 7393
Size: 279 384 21 519 180 1129 326 2583
Cuts at: 7393 7760 8145 8162 9614 9700 9712 10058 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2583 1977 1452 1129 519 385 384 367
346 326 279 180 86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 4335 4882 4335
Size: 547 9516
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 3063 3111 3825 4155 4674
Size: 552 939 114 945 48 714 330 519
Cuts at: 4674 5445 6336 513
Size: 771 891 4240
Fragments arranged by size:
4240 945 939 891 771 714 552 519
330 114 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9324
Fragments arranged by size:
9324 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 3137 3355 3436 4559 4741 5315 6774 7579
Size: 244 218 81 1123 182 574 1459 805
Cuts at: 7579 8373 8393 2893
Size: 794 20 4563
Fragments arranged by size:
4563 1459 1123 805 794 574 244 218
182 81 20
ClaI AT'CG_AT
Cuts at: 1554 4107 1554
Size: 2553 7510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10028
DraI TTT'AAA
Cuts at: 7367 7367
Size: 10063
DraIII CAC_nnn'GTG
Cuts at: 5435 5489 5435
Size: 54 10009
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6353 9070 809
Size: 507 5037 2717 1802
Fragments arranged by size:
5037 2717 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 3060 3274
Size: 54 1425 50 275 18 307 923 214
Cuts at: 3274 4007 4516 4615 5229 7510 8399 10028 8
Size: 733 509 99 614 2281 889 1629 43
Fragments arranged by size:
2281 1629 1425 923 889 733 614 509
307 275 214 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2137 4007 7510 1537
Size: 275 325 1870 3503 4090
Fragments arranged by size:
4090 3503 1870 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5369 7128 7993 1353
Size: 4016 1759 865 3423
Fragments arranged by size:
4016 3423 1759 865
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8650 9024 9170 387
Size: 1064 138 7061 374 146 1280
Fragments arranged by size:
7061 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2628 2997 3426 3777 5627 6393
Size: 393 368 628 369 429 351 1850 766
Cuts at: 6393 6423 6462 6671 7152 8126 8723 9510 9778
Size: 30 39 209 481 974 597 787 268
Cuts at: 9778 1239
Size: 1524
Fragments arranged by size:
1850 1524 974 787 766 628 597 481
429 393 369 368 351 268 209 39
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2976 5069 2976
Size: 2093 7970
EcoO109I rG'GnC_Cy
Cuts at: 3698 6213 6284 3698
Size: 2515 71 7477
Fragments arranged by size:
7477 2515 71
EcoRI G'AATT_C
Cuts at: 2393 7372 7750 2393
Size: 4979 378 4706
Fragments arranged by size:
4979 4706 378
FspI TGC'GCA
Cuts at: 2095 8569 2095
Size: 6474 3589
HaeII r_GCGC'y
Cuts at: 1678 9210 1678
Size: 7532 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2257 2289 2419 2451 3601
Size: 32 135 32 685 32 130 32 1150
Cuts at: 3601 3633 3981 4013 4096 4128 4865 4897 6080
Size: 32 348 32 83 32 737 32 1183
Cuts at: 6080 6112 8704 8736 8778 8810 1373
Size: 32 2592 32 42 32 2626
Fragments arranged by size:
2626 2592 1183 1150 737 685 348 135
130 83 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3527 7407 7653 669
Size: 988 281 1589 3880 246 3079
Fragments arranged by size:
3880 3079 1589 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2150 748
Size: 1402 8661
HpaI GTT'AAC
Cuts at: 7653 7653
Size: 10063
KpnI G_GTAC'C
Cuts at: 7388 7388
Size: 10063
MfeI C'AATT_G
Cuts at: 7662 7662
Size: 10063
MluI A'CGCG_T
Cuts at: 6933 9939 6933
Size: 3006 7057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2398 3941 4029 4381 9177 9361 1305
Size: 1093 1543 88 352 4796 184 2007
Fragments arranged by size:
4796 2007 1543 1093 352 184 88
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3062 3276 4518 4617 5231
Size: 54 1425 343 1230 214 1242 99 614
Cuts at: 5231 10
Size: 4842
Fragments arranged by size:
4842 1425 1242 1230 614 343 214 99
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3315 3893 4623 4686 8021 8380 8539
Size: 648 2149 578 730 63 3335 359 159
Cuts at: 8539 518
Size: 2042
Fragments arranged by size:
3335 2149 2042 730 648 578 359 159
63
MspA1I CmG'CkG
Cuts at: 1606 2106 2441 3078 3212 5205 5227 6182 7142
Size: 500 335 637 134 1993 22 955 960
Cuts at: 7142 7479 8156 9304 9549 1606
Size: 337 677 1148 245 2120
Fragments arranged by size:
2120 1993 1148 960 955 677 637 500
337 335 245 134 22
NaeI GCC'GGC
Cuts at: 3599 3763 4388 3599
Size: 164 625 9274
Fragments arranged by size:
9274 625 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 3111 3825 4674 6336 513
Size: 552 939 1107 714 849 1662 4240
Fragments arranged by size:
4240 1662 1107 939 849 714 552
NdeI CA'TA_TG
Cuts at: 387 2237 387
Size: 1850 8213
NgoMIV G'CCGG_C
Cuts at: 3597 3761 4386 3597
Size: 164 625 9274
Fragments arranged by size:
9274 625 164
NheI G'CTAG_C
Cuts at: 1052 2023 1052
Size: 971 9092
NotI GC'GGCC_GC
Cuts at: 7510 7510
Size: 10063
NspI r_CATG'y
Cuts at: 1132 1229 2902 5323 7359 7421 8966 1132
Size: 97 1673 2421 2036 62 1545 2229
Fragments arranged by size:
2421 2229 2036 1673 1545 97 62
PasI CC'CwG_GG
Cuts at: 1348 5412 6209 1348
Size: 4064 797 5202
Fragments arranged by size:
5202 4064 797
PciI A'CATG_T
Cuts at: 8962 8962
Size: 10063
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4643 5220 1108
Size: 3535 577 5951
Fragments arranged by size:
5951 3535 577
PfoI T'CCnGG_A
Cuts at: 2753 3403 3732 3749 4846 5604 2753
Size: 650 329 17 1097 758 7212
Fragments arranged by size:
7212 1097 758 650 329 17
PmeI GTTT'AAAC
Cuts at: 7367 7367
Size: 10063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8132 8164 9645 9677 8132
Size: 32 1481 32 8518
Fragments arranged by size:
8518 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10063
PsiI TTA'TAA
Cuts at: 7633 7633
Size: 10063
PstI C_TGCA'G
Cuts at: 830 5348 6254 6781 7415 830
Size: 4518 906 527 634 3478
Fragments arranged by size:
4518 3478 906 634 527
PvuI CG_AT'CG
Cuts at: 2001 2462 2001
Size: 461 9602
PvuII CAG'CTG
Cuts at: 2106 2441 3212 5227 7142 2106
Size: 335 771 2015 1915 5027
Fragments arranged by size:
5027 2015 1915 771 335
SalI G'TCGA_C
Cuts at: 1936 3525 7405 1936
Size: 1589 3880 4594
Fragments arranged by size:
4594 3880 1589
SbfI CC_TGCA'GG
Cuts at: 7415 7415
Size: 10063
ScaI AGT'ACT
Cuts at: 8311 10023 8311
Size: 1712 8351
SexAI A'CCwGG_T
Cuts at: 3637 3960 3637
Size: 323 9740
SfcI C'TryA_G
Cuts at: 826 1045 5344 5867 6250 6777 7411 8546 9227
Size: 219 4299 523 383 527 634 1135 681
Cuts at: 9227 9418 826
Size: 191 1471
Fragments arranged by size:
4299 1471 1135 681 634 527 523 383
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10063
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10063
SmaI CCC'GGG
Cuts at: 1872 7390 1872
Size: 5518 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2701 3502 3985 4997 5799
Size: 197 926 384 374 801 483 1012 802
Cuts at: 5799 5990 6669 6977 7197 7278 8167 9068 9330
Size: 191 679 308 220 81 889 901 262
Cuts at: 9330 820
Size: 1553
Fragments arranged by size:
1553 1012 926 901 889 802 801 679
483 384 374 308 262 220 197 191
81
SnaBI TAC'GTA
Cuts at: 493 7854 493
Size: 7361 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10063
SphI G_CATG'C
Cuts at: 1132 5323 7421 1132
Size: 4191 2098 3774
Fragments arranged by size:
4191 3774 2098
SspI AAT'ATT
Cuts at: 5 52 7987 5
Size: 47 7935 2081
Fragments arranged by size:
7935 2081 47
StuI AGG'CCT
Cuts at: 1517 2519 4314 5621 1517
Size: 1002 1795 1307 5959
Fragments arranged by size:
5959 1795 1307 1002
StyI C'CwwG_G
Cuts at: 513 1065 2004 2669 2916 2963 3111 3544 3825
Size: 552 939 665 247 47 148 433 281
Cuts at: 3825 4674 5616 5955 6336 7502 513
Size: 849 942 339 381 1166 3074
Fragments arranged by size:
3074 1166 942 939 849 665 552 433
381 339 281 247 148 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3171 8090 8107 8260 8445 8890 325
Size: 1255 1591 4919 17 153 185 445 1498
Fragments arranged by size:
4919 1591 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4062 8309 10021
Size: 274 80 33 51 1443 2085 4247 1712
Cuts at: 10021 96
Size: 138
Fragments arranged by size:
4247 2085 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4161 4193 5586 5618 10023 10055 4161
Size: 32 1393 32 4405 32 4169
Fragments arranged by size:
4405 4169 1393 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10063
XbaI T'CTAG_A
Cuts at: 4222 7399 4222
Size: 3177 6886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5321 6037 6478 5321
Size: 716 441 8906
Fragments arranged by size:
8906 716 441
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10063
XmaI C'CCGG_G
Cuts at: 1870 7388 1870
Size: 5518 4545
XmnI GAAnn'nnTTC
Cuts at: 4203 6447 6861 8192 4203
Size: 2244 414 1331 6074
Fragments arranged by size:
6074 2244 1331 414
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 7871 276
Size: 53 83 186 936 2083 4254 2468
Fragments arranged by size:
4254 2468 2083 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI