Restriction map for
pFN21AB3103
(Circular) (Six-base) MAPSORT of: pFN21AB3103.seq Check: 9474 from: 1 to: 6355
pFN21AB3103 6355 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2335 2335
Size: 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2511 3698 1937
Size: 574 1187 4594
Fragments arranged by size:
4594 1187 574
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2001 4418 5802 6070 1632
Size: 369 2417 1384 268 1917
Fragments arranged by size:
2417 1917 1384 369 268
AflII C'TTAA_G
Cuts at: 820 1017 2210 820
Size: 197 1193 4965
Fragments arranged by size:
4965 1193 197
AflIII A'CryG_T
Cuts at: 1203 5254 6231 1203
Size: 4051 977 1327
Fragments arranged by size:
4051 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 2990 1166
Size: 1824 4531
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AlwNI CAG_nnn'CTG
Cuts at: 3020 3388 3407 4098 5670 3020
Size: 368 19 691 1572 3705
Fragments arranged by size:
3705 1572 691 368 19
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2264 2708 2819 3664 3890 4042 6151 1493
Size: 771 444 111 845 226 152 2109 1697
Fragments arranged by size:
2109 1697 845 771 444 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3253 3430 3680 1870
Size: 73 1310 177 250 4545
Fragments arranged by size:
4545 1310 250 177 73
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2148 3676 5131 618
Size: 325 217 627 336 25 1528 1455 1842
Fragments arranged by size:
1842 1528 1455 627 336 325 217 25
BanII G_rGCy'C
Cuts at: 1391 2144 3127 3260 3336 3624 3674 1391
Size: 753 983 133 76 288 50 4072
Fragments arranged by size:
4072 983 753 288 133 76 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2441 3017 928
Size: 971 542 576 4266
Fragments arranged by size:
4266 971 576 542
BbvCI CC'TCA_GC
Cuts at: 2177 3228 2177
Size: 1051 5304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2930 2964 4546 4580 1903
Size: 34 993 34 1582 34 3678
Fragments arranged by size:
3678 1582 993 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 2401 1548
Size: 853 5502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3384 3404 4966
Size: 107 122 71 631 605 1712 20 1562
Cuts at: 4966 136
Size: 1525
Fragments arranged by size:
1712 1562 1525 631 605 122 107 71
20
BglII A'GATC_T
Cuts at: 3534 6350 3534
Size: 2816 3539
BlpI GC'TnA_GC
Cuts at: 3296 3772 3296
Size: 476 5879
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2746 4416 5572 1790
Size: 361 595 1670 1156 2573
Fragments arranged by size:
2573 1670 1156 595 361
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 4086 5044
Size: 416 53 83 399 24 660 2009 958
Cuts at: 5044 442
Size: 1753
Fragments arranged by size:
2009 1753 958 660 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2452 3197 5015 1239
Size: 1213 745 1818 2579
Fragments arranged by size:
2579 1818 1213 745
Bpu10I CC'TnA_GC
Cuts at: 2177 3078 3228 2177
Size: 901 150 5304
Fragments arranged by size:
5304 901 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 5018 882
Size: 617 567 2952 2219
Fragments arranged by size:
2952 2219 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 4146 493
Size: 711 2942 2702
Fragments arranged by size:
2942 2702 711
BsaBI GATnn'nnATC
Cuts at: 6349 6349
Size: 6355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4162 4544 5179 275
Size: 53 83 186 936 2629 382 635 1451
Fragments arranged by size:
2629 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4788 5186 5460 5607 6018 4788
Size: 398 274 147 411 5125
Fragments arranged by size:
5125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3323 3353 1178
Size: 30 165 30 1920 30 4180
Fragments arranged by size:
4180 1920 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3194 3254 1186
Size: 2008 60 4287
Fragments arranged by size:
4287 2008 60
BseYI C'CCAG_C
Cuts at: 2751 2898 3126 3628 5558 6054 2751
Size: 147 228 502 1930 496 3052
Fragments arranged by size:
3052 1930 502 496 228 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2516 2738 2960 1163
Size: 573 420 360 222 222 4558
Fragments arranged by size:
4558 573 420 360 222 222
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2506 3805 4566 5594 1540
Size: 275 186 505 1299 761 1028 2301
Fragments arranged by size:
2301 1299 1028 761 505 275 186
BsiHKAI G_wGCw'C
Cuts at: 4416 4501 5572 4416
Size: 85 1071 5199
Fragments arranged by size:
5199 1071 85
BsmI GAATG_Cn'
Cuts at: 2968 3866 3959 2968
Size: 898 93 5364
Fragments arranged by size:
5364 898 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2151 2746 3127 3260 3336 3624
Size: 399 354 7 595 381 133 76 288
Cuts at: 3624 3674 4416 4501 5572 1391
Size: 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 399 381 354 288
133 85 76 50 7
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 4244 5974 4244
Size: 1730 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2335 3643 3710 844
Size: 1491 1308 67 3489
Fragments arranged by size:
3489 1491 1308 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3145 4242 1394
Size: 429 1322 1097 3507
Fragments arranged by size:
3507 1322 1097 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4850 5024 65
Size: 1183 3602 174 1396
Fragments arranged by size:
3602 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3612 4999 5186 1700
Size: 110 1802 1387 187 2869
Fragments arranged by size:
2869 1802 1387 187 110
BsrGI T'GTAC_A
Cuts at: 96 3339 96
Size: 3243 3112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 2558 2669 2891 4415 5427 2558
Size: 111 222 1524 1012 3486
Fragments arranged by size:
3486 1524 1012 222 111
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6355
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 2255 3640 2255
Size: 1385 4970
BstYI r'GATC_y
Cuts at: 1972 3534 3685 4052 4437 4454 5906 5992 6004
Size: 1562 151 367 385 17 1452 86 12
Cuts at: 6004 6350 1972
Size: 346 1977
Fragments arranged by size:
1977 1562 1452 385 367 346 151 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2248 2359 513
Size: 552 1183 111 4509
Fragments arranged by size:
4509 1183 552 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2023 3540 487
Size: 683 56 797 1517 3302
Fragments arranged by size:
3302 1517 797 683 56
BtsI GCAGTG_nn'
Cuts at: 2335 3871 4665 4685 2335
Size: 1536 794 20 4005
Fragments arranged by size:
4005 1536 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DraIII CAC_nnn'GTG
Cuts at: 2032 2032
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2305 2883 3135
Size: 54 1425 50 275 18 475 578 252
Cuts at: 3135 3802 4691 6320 8
Size: 667 889 1629 43
Fragments arranged by size:
1629 1425 889 667 578 475 275 252
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3153 3629 4285 1353
Size: 1800 476 656 3423
Fragments arranged by size:
3423 1800 656 476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3118 3125 3131 4942 5316 5462
Size: 1064 138 1529 7 6 1811 374 146
Cuts at: 5462 387
Size: 1280
Fragments arranged by size:
1811 1529 1280 1064 374 146 138 7
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 2452 3197 4418 5015 5802 6070
Size: 393 369 451 745 1221 597 787 268
Cuts at: 6070 1239
Size: 1524
Fragments arranged by size:
1524 1221 787 745 597 451 393 369
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3077 3077
Size: 6355
EcoO109I rG'GnC_Cy
Cuts at: 3308 3385 3427 3308
Size: 77 42 6236
Fragments arranged by size:
6236 77 42
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 2634 5502 1678
Size: 956 2868 2531
Fragments arranged by size:
2868 2531 956
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3521 3553 4996 5028 5070
Size: 32 135 32 1949 32 1443 32 42
Cuts at: 5070 5102 1373
Size: 32 2626
Fragments arranged by size:
2626 1949 1443 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3699 3945 669
Size: 988 281 1032 729 246 3079
Fragments arranged by size:
3079 1032 988 729 281 246
HindIII A'AGCT_T
Cuts at: 748 2013 2241 748
Size: 1265 228 4862
Fragments arranged by size:
4862 1265 228
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2814 5469 5653 1305
Size: 1509 2655 184 2007
Fragments arranged by size:
2655 2007 1509 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2885 3137 10
Size: 54 1425 343 1053 252 3228
Fragments arranged by size:
3228 1425 1053 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 2990 3572 4313 4672 4831 518
Size: 648 1087 737 582 741 359 159 2042
Fragments arranged by size:
2042 1087 741 737 648 582 359 159
MspA1I CmG'CkG
Cuts at: 1606 2300 2776 2852 3130 3356 3771 4448 5596
Size: 694 476 76 278 226 415 677 1148
Cuts at: 5596 5841 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 694 677 476 415 278 245
226 76
NcoI C'CATG_G
Cuts at: 513 1065 2248 513
Size: 552 1183 4620
Fragments arranged by size:
4620 1183 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2450 3713 5258 1132
Size: 97 1221 1263 1545 2229
Fragments arranged by size:
2229 1545 1263 1221 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6355
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3133 1108
Size: 2025 4330
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 2799 3925 2799
Size: 1126 5229
PspXI vC'TCGA_Gb
Cuts at: 3253 3253
Size: 6355
PstI C_TGCA'G
Cuts at: 830 3707 830
Size: 2877 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
PvuII CAG'CTG
Cuts at: 2300 2776 2300
Size: 476 5879
RsrII CG'GwC_CG
Cuts at: 2356 2356
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SapI GCTCTTCn'nnn_
Cuts at: 3629 3629
Size: 6355
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SfcI C'TryA_G
Cuts at: 826 1045 2412 3301 3703 4838 5519 5710 826
Size: 219 1367 889 402 1135 681 191 1471
Fragments arranged by size:
1471 1367 1135 889 681 402 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3432 3682 1872
Size: 1560 250 4545
Fragments arranged by size:
4545 1560 250
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 3253 4459 5360 5622 820
Size: 197 926 267 1043 1206 901 262 1553
Fragments arranged by size:
1553 1206 1043 926 901 267 262 197
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 2450 3713 1132
Size: 1318 1263 3774
Fragments arranged by size:
3774 1318 1263
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6355
StyI C'CwwG_G
Cuts at: 513 1065 2248 2746 3138 3794 513
Size: 552 1183 498 392 656 3074
Fragments arranged by size:
3074 1183 656 552 498 392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 2298 2344 2520 3002 4382 4399
Size: 1255 510 208 46 176 482 1380 17
Cuts at: 4399 4552 4737 5182 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1380 1255 510 482 445 208 185
176 153 46 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3339 4601 6313
Size: 274 80 33 51 1443 1362 1262 1712
Cuts at: 6313 96
Size: 138
Fragments arranged by size:
1712 1443 1362 1262 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2018 2050 2254 2286 6315 6347 2018
Size: 32 204 32 4029 32 2026
Fragments arranged by size:
4029 2026 204 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2354 1316
Size: 1038 5317
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3172 3172
Size: 6355
XhoI C'TCGA_G
Cuts at: 1943 3253 1943
Size: 1310 5045
XmaI C'CCGG_G
Cuts at: 1870 3430 3680 1870
Size: 1560 250 4545
Fragments arranged by size:
4545 1560 250
XmnI GAAnn'nnTTC
Cuts at: 4484 4484
Size: 6355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII RsrII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PsrI SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI