Restriction map for
pFN21AB3086
(Circular) (Six-base) MAPSORT of: pFN21AB3086.seq Check: 6270 from: 1 to: 6445
pFN21AB3086 6445 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2855 2855
Size: 6445
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 1937
Size: 1851 4594
Acc65I G'GTAC_C
Cuts at: 3766 3766
Size: 6445
AclI AA'CG_TT
Cuts at: 1563 3675 4572 4945 1563
Size: 2112 897 373 3063
Fragments arranged by size:
3063 2112 897 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2678 2762 3114 3422 3710 4508 5892
Size: 635 411 84 352 308 288 798 1384
Cuts at: 5892 6160 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 798 635 411 352 308 288
268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 2456 1166
Size: 1290 5155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6445
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2291 2323 3665 3697 2291
Size: 32 1342 32 5039
Fragments arranged by size:
5039 1342 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2137 2701 2806 3421 4188 5760 2137
Size: 564 105 615 767 1572 2822
Fragments arranged by size:
2822 1572 767 615 564 105
ApaI G_GGCC'C
Cuts at: 2213 3368 2213
Size: 1155 5290
ApaLI G'TGCA_C
Cuts at: 3740 4502 5658 3740
Size: 762 1156 4527
Fragments arranged by size:
4527 1156 762
ApoI r'AATT_y
Cuts at: 1493 2444 2632 3754 3980 4132 6241 1493
Size: 951 188 1122 226 152 2109 1697
Fragments arranged by size:
2109 1697 1122 951 226 188 152
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 3668 3770 1870
Size: 73 122 1603 102 4545
Fragments arranged by size:
4545 1603 122 102 73
BamHI G'GATC_C
Cuts at: 3775 4142 3775
Size: 367 6078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2158 3766 5221 618
Size: 325 217 627 371 1608 1455 1842
Fragments arranged by size:
1842 1608 1455 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 2213 2304 3368 3764 1391
Size: 822 91 1064 396 4072
Fragments arranged by size:
4072 1064 822 396 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4636 4670 1903
Size: 34 2699 34 3678
Fragments arranged by size:
3678 2699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 2721 2734 1548
Size: 1173 13 5259
Fragments arranged by size:
5259 1173 13
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5056 136
Size: 107 122 71 631 605 3384 1525
Fragments arranged by size:
3384 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3077 3097 6440 3077
Size: 20 3343 3082
Fragments arranged by size:
3343 3082 20
BlpI GC'TnA_GC
Cuts at: 2051 3862 2051
Size: 1811 4634
Bme1580I G_kGCm'C
Cuts at: 1790 2213 3055 3368 3744 4506 5662 1790
Size: 423 842 313 376 762 1156 2573
Fragments arranged by size:
2573 1156 842 762 423 376 313
BmgBI CAC'GTC
Cuts at: 1318 2612 1318
Size: 1294 5151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4176 5134 442
Size: 416 53 83 399 24 2759 958 1753
Fragments arranged by size:
2759 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2911 5105 1239
Size: 1672 2194 2579
Fragments arranged by size:
2579 2194 1672
Bpu10I CC'TnA_GC
Cuts at: 3369 3369
Size: 6445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2209 2288 4570 5435 5733 2135
Size: 74 79 2282 865 298 2847
Fragments arranged by size:
2847 2282 865 298 79 74
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2975 5108 882
Size: 617 1476 2133 2219
Fragments arranged by size:
2219 2133 1476 617
BsaAI yAC'GTr
Cuts at: 493 1204 4236 493
Size: 711 3032 2702
Fragments arranged by size:
3032 2702 711
BsaBI GATnn'nnATC
Cuts at: 6439 6439
Size: 6445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 4252 4634 5269
Size: 53 83 186 936 567 2152 382 635
Cuts at: 5269 275
Size: 1451
Fragments arranged by size:
2152 1451 936 635 567 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4878 5276 5550 5697 6108 4878
Size: 398 274 147 411 5215
Fragments arranged by size:
5215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2291 2321 1178
Size: 30 165 30 888 30 5302
Fragments arranged by size:
5302 888 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2616 1186
Size: 1430 5015
BseYI C'CCAG_C
Cuts at: 2544 5648 6144 2544
Size: 3104 496 2845
Fragments arranged by size:
3104 2845 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5872
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 3744 4506 4591 5662 2304
Size: 1440 762 85 1071 3087
Fragments arranged by size:
3087 1440 1071 762 85
BsmI GAATG_Cn'
Cuts at: 2329 2772 3019 3956 4049 2329
Size: 443 247 937 93 4725
Fragments arranged by size:
4725 937 443 247 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2304 3055 3368 3744 3764 4506
Size: 399 423 91 751 313 376 20 742
Cuts at: 4506 4591 5662 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 751 742 423 399 376 313
91 85 20
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 4334 6064 4334
Size: 1730 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 3800 844
Size: 2011 945 3489
Fragments arranged by size:
3489 2011 945
BsrBI CCG'CTC
Cuts at: 1394 1823 2121 4332 1394
Size: 429 298 2211 3507
Fragments arranged by size:
3507 2211 429 298
BsrDI GCAATG_nn'
Cuts at: 65 1248 2845 4940 5114 65
Size: 1183 1597 2095 174 1396
Fragments arranged by size:
2095 1597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5089 5276 1700
Size: 110 3279 187 2869
Fragments arranged by size:
3279 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2934 96
Size: 2838 3607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6445
BssSI C'ACGA_G
Cuts at: 3287 4505 5517 3287
Size: 1218 1012 4215
Fragments arranged by size:
4215 1218 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2028 794
Size: 1234 5211
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6445
BstXI CCAn_nnnn'nTGG
Cuts at: 3718 3718
Size: 6445
BstYI r'GATC_y
Cuts at: 1972 2685 3077 3097 3775 4142 4527 4544 5996
Size: 713 392 20 678 367 385 17 1452
Cuts at: 5996 6082 6094 6440 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 713 678 392 385 367 346
86 20 17 12
Bsu36I CC'TnA_GG
Cuts at: 2205 2205
Size: 6445
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4954
Fragments arranged by size:
4954 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2057 487
Size: 683 56 831 4875
Fragments arranged by size:
4875 831 683 56
BtsI GCAGTG_nn'
Cuts at: 2669 3961 4755 4775 2669
Size: 1292 794 20 4339
Fragments arranged by size:
4339 1292 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3019 3054 529
Size: 35 2455 35 3920
Fragments arranged by size:
3920 2455 35 35
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DraIII CAC_nnn'GTG
Cuts at: 2028 2028
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5452 809
Size: 507 4136 1802
Fragments arranged by size:
4136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2761 3708
Size: 54 1425 50 275 18 177 754 947
Cuts at: 3708 3892 4781 6410 8
Size: 184 889 1629 43
Fragments arranged by size:
1629 1425 947 889 754 275 184 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4375 1353
Size: 3022 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3266 5032 5406 5552 387
Size: 1064 138 1677 1766 374 146 1280
Fragments arranged by size:
1766 1677 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2302 2302
Size: 6445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2678 2762 2911 3114 3422 3710
Size: 393 635 411 84 149 203 308 288
Cuts at: 3710 4508 5105 5892 6160 1239
Size: 798 597 787 268 1524
Fragments arranged by size:
1524 798 787 635 597 411 393 308
288 268 203 149 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3470 3470
Size: 6445
EcoO109I rG'GnC_Cy
Cuts at: 2209 3364 3365 2209
Size: 1155 1 5289
Fragments arranged by size:
5289 1155 1
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
FspI TGC'GCA
Cuts at: 2021 4951 2021
Size: 2930 3515
FspAI rTGC'GCAy
Cuts at: 2021 2021
Size: 6445
HaeII r_GCGC'y
Cuts at: 1678 5592 1678
Size: 3914 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3270 3302 5086 5118 5160
Size: 32 135 32 1698 32 1784 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2626 1784 1698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2424 2642 3692 748
Size: 1676 218 1050 3501
Fragments arranged by size:
3501 1676 1050 218
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KpnI G_GTAC'C
Cuts at: 3770 3770
Size: 6445
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5559 5743 1305
Size: 4254 184 2007
Fragments arranged by size:
4254 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3710 10
Size: 54 1425 343 1878 2745
Fragments arranged by size:
2745 1878 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 2777 3434 3704 3716 4403 4762
Size: 648 1290 321 657 270 12 687 359
Cuts at: 4762 4921 518
Size: 159 2042
Fragments arranged by size:
2042 1290 687 657 648 359 321 270
159 12
MspA1I CmG'CkG
Cuts at: 1606 2278 3688 3861 4538 5686 5931 1606
Size: 672 1410 173 677 1148 245 2120
Fragments arranged by size:
2120 1410 1148 677 672 245 173
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4954
Fragments arranged by size:
4954 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 2220 2935 3803 5348 1132
Size: 97 991 715 868 1545 2229
Fragments arranged by size:
2229 1545 991 868 715 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6445
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2781 1108
Size: 1673 4772
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2323 4514 4546 6027 6059 2291
Size: 32 2191 32 1481 32 2677
Fragments arranged by size:
2677 2191 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PspOMI G'GGCC_C
Cuts at: 2209 3364 2209
Size: 1155 5290
PstI C_TGCA'G
Cuts at: 830 2142 3236 3797 830
Size: 1312 1094 561 3478
Fragments arranged by size:
3478 1312 1094 561
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2278 2278
Size: 6445
SacI G_AGCT'C
Cuts at: 2304 2304
Size: 6445
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 2888 4693 6405 2888
Size: 1805 1712 2928
Fragments arranged by size:
2928 1805 1712
SfcI C'TryA_G
Cuts at: 826 1045 2138 3208 3232 3793 4928 5609 5800
Size: 219 1093 1070 24 561 1135 681 191
Cuts at: 5800 826
Size: 1471
Fragments arranged by size:
1471 1135 1093 1070 681 561 219 191
24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 2067 3772 1872
Size: 195 1705 4545
Fragments arranged by size:
4545 1705 195
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2224 2303 4549 5450 5712
Size: 197 926 207 74 79 2246 901 262
Cuts at: 5712 820
Size: 1553
Fragments arranged by size:
2246 1553 926 901 262 207 197 79
74
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 2345 2562 1517
Size: 828 217 5400
Fragments arranged by size:
5400 828 217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2577 3884 513
Size: 552 939 573 1307 3074
Fragments arranged by size:
3074 1307 939 573 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 2429 4472 4489 4642 4827 5272
Size: 1255 566 283 2043 17 153 185 445
Cuts at: 5272 325
Size: 1498
Fragments arranged by size:
2043 1498 1255 566 445 283 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2886 2934 4691
Size: 274 80 33 51 1443 909 48 1757
Cuts at: 4691 6403 96
Size: 1712 138
Fragments arranged by size:
1757 1712 1443 909 274 138 80 51
48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2703 2735 6405 6437 2703
Size: 32 3670 32 2711
Fragments arranged by size:
3670 2711 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 1316
Size: 1185 5260
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 2065 3770 1870
Size: 195 1705 4545
Fragments arranged by size:
4545 1705 195
XmnI GAAnn'nnTTC
Cuts at: 2901 3177 3194 4574 2901
Size: 276 17 1380 4772
Fragments arranged by size:
4772 1380 276 17
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI