Restriction map for
pFN21AB3085
(Circular) (Six-base) MAPSORT of: pFN21AB3085.seq Check: 2516 from: 1 to: 5338
pFN21AB3085 5338 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 2249 1184
Size: 915 150 4273
Fragments arranged by size:
4273 915 150
AlwNI CAG_nnn'CTG
Cuts at: 3081 4653 3081
Size: 1572 3766
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2444 2483 2647 2873 3025 5134 1493
Size: 951 39 164 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 226 164 152 39
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 1870
Size: 73 720 4545
Fragments arranged by size:
4545 720 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 2540 2659 4114 618
Size: 325 217 627 276 477 119 1455 1842
Fragments arranged by size:
1842 1455 627 477 325 276 217 119
BanII G_rGCy'C
Cuts at: 1391 2657 1391
Size: 1266 4072
BbeI G_GCGC'C
Cuts at: 2067 2067
Size: 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 928
Size: 971 438 3929
Fragments arranged by size:
3929 971 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2416 2450 3529 3563 1903
Size: 34 479 34 1079 34 3678
Fragments arranged by size:
3678 1079 479 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3949 136
Size: 107 122 71 631 605 2277 1525
Fragments arranged by size:
2277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2545 3399 4555 1790
Size: 755 854 1156 2573
Fragments arranged by size:
2573 1156 854 755
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 2459 3069
Size: 416 53 83 399 24 666 376 610
Cuts at: 3069 4027 442
Size: 958 1753
Fragments arranged by size:
1753 958 666 610 416 399 376 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3998 1239
Size: 2759 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3129 493
Size: 711 1431 494 2702
Fragments arranged by size:
2702 1431 711 494
BsaBI GATnn'nnATC
Cuts at: 2285 5332 2285
Size: 3047 2291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 3145 3527 4162
Size: 53 83 186 936 531 1081 382 635
Cuts at: 4162 275
Size: 1451
Fragments arranged by size:
1451 1081 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 2508 4541 5037 2508
Size: 2033 496 2809
Fragments arranged by size:
2809 2033 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 2595 1163
Size: 573 627 232 3906
Fragments arranged by size:
3906 627 573 232
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2180 3399 3484 4555 2180
Size: 1219 85 1071 2963
Fragments arranged by size:
2963 1219 1071 85
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2545 2657 3399 3484 4555 1391
Size: 399 390 365 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 365 112 85
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 2617 2693 844
Size: 1537 236 76 3489
Fragments arranged by size:
3489 1537 236 76
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2231 3833 4007 65
Size: 1183 983 1602 174 1396
Fragments arranged by size:
1602 1396 1183 983 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 3982 4169 1700
Size: 110 301 1871 187 2869
Fragments arranged by size:
2869 1871 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2246 794
Size: 1452 3886
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2187 2668 3035 3420 3437 4889 4975 4987
Size: 215 481 367 385 17 1452 86 12
Cuts at: 4987 5333 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 481 385 367 346 215 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3847
Fragments arranged by size:
3847 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2854 3648 3668 2076
Size: 778 794 20 3746
Fragments arranged by size:
3746 794 778 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2274 2309 529
Size: 35 1710 35 3558
Fragments arranged by size:
3558 1710 35 35
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2295 2785
Size: 54 1425 50 275 18 177 288 490
Cuts at: 2785 3674 5303 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 490 288 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 3268 1353
Size: 1151 764 3423
Fragments arranged by size:
3423 1151 764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3925 4299 4445 387
Size: 1064 138 2336 374 146 1280
Fragments arranged by size:
2336 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3401 3998 4785 5053 1239
Size: 393 1769 597 787 268 1524
Fragments arranged by size:
1769 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2595 2595
Size: 5338
EcoRI G'AATT_C
Cuts at: 2444 2647 3025 2444
Size: 203 378 4757
Fragments arranged by size:
4757 378 203
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2067 4485 1678
Size: 389 2418 2531
Fragments arranged by size:
2531 2418 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373
Size: 32 135 32 2407 32 42 32 2626
Fragments arranged by size:
2626 2407 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KasI G'GCGC_C
Cuts at: 2063 2063
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2297 10
Size: 54 1425 343 177 288 3051
Fragments arranged by size:
3051 1425 343 288 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3296 3655 3814 518
Size: 648 2130 359 159 2042
Fragments arranged by size:
2130 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2622 2754 3431 4579 4824 1606
Size: 420 596 132 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 596 420 245 132
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 5338
NarI GG'CG_CC
Cuts at: 2064 2064
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3847
Fragments arranged by size:
3847 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2418 1108
Size: 1310 4028
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2465 1613
Size: 852 4486
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2025 2690 830
Size: 1195 665 3478
Fragments arranged by size:
3478 1195 665
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2026 2026
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2021 2686 3821 4502 4693 826
Size: 219 976 665 1135 681 191 1471
Fragments arranged by size:
1471 1135 976 681 665 219 191
SfoI GGC'GCC
Cuts at: 2065 2065
Size: 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2591 2777 513
Size: 552 939 587 186 3074
Fragments arranged by size:
3074 939 587 552 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 3382 3535 3720 4165 325
Size: 1255 1785 17 153 185 445 1498
Fragments arranged by size:
1785 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI