Restriction map for
pFN21AB3078
(Circular) (Six-base) MAPSORT of: pFN21AB3078.seq Check: 4486 from: 1 to: 7528
pFN21AB3078 7528 bp
With 182 enzymes: SgfI *
September 11, 2009 17:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2869 2869
Size: 7528
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5338 278
Size: 53 83 186 936 3802 2468
Fragments arranged by size:
3802 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4092 4871 1937
Size: 2155 779 4594
Fragments arranged by size:
4594 2155 779
Acc65I G'GTAC_C
Cuts at: 4849 4849
Size: 7528
AclI AA'CG_TT
Cuts at: 1563 5655 6028 1563
Size: 4092 373 3063
Fragments arranged by size:
4092 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2753 5591 6975 7243 1632
Size: 953 168 2838 1384 268 1917
Fragments arranged by size:
2838 1917 1384 953 268 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7331
AflIII A'CryG_T
Cuts at: 1203 2817 4789 6427 7404 1203
Size: 1614 1972 1638 977 1327
Fragments arranged by size:
1972 1638 1614 1327 977
AgeI A'CCGG_T
Cuts at: 6359 6359
Size: 7528
AhdI GACnn_n'nnGTC
Cuts at: 6257 6257
Size: 7528
AleI CACnn'nnGTG
Cuts at: 1166 4340 1166
Size: 3174 4354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7528
AlwNI CAG_nnn'CTG
Cuts at: 3675 3793 4755 5271 6843 3675
Size: 118 962 516 1572 4360
Fragments arranged by size:
4360 1572 962 516 118
ApaI G_GGCC'C
Cuts at: 3057 3057
Size: 7528
ApaLI G'TGCA_C
Cuts at: 2882 5585 6741 2882
Size: 2703 1156 3669
Fragments arranged by size:
3669 2703 1156
ApoI r'AATT_y
Cuts at: 1493 3284 4837 5063 5215 7324 1493
Size: 1791 1553 226 152 2109 1697
Fragments arranged by size:
2109 1791 1697 1553 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6082 160
Size: 872 5050 1606
Fragments arranged by size:
5050 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2114 3476 4143 4819 4853 1870
Size: 73 90 81 1362 667 676 34 4545
Fragments arranged by size:
4545 1362 676 667 90 81 73 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3817 3850 3817
Size: 33 7495
BamHI G'GATC_C
Cuts at: 3156 3383 3626 4858 5225 3156
Size: 227 243 1232 367 5459
Fragments arranged by size:
5459 1232 367 243 227
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2138 3147 4849 6304
Size: 325 217 627 323 28 1009 1702 1455
Cuts at: 6304 618
Size: 1842
Fragments arranged by size:
1842 1702 1455 1009 627 325 323 217
28
BanII G_rGCy'C
Cuts at: 1391 2024 2967 2975 3057 4506 4617 4847 1391
Size: 633 943 8 82 1449 111 230 4072
Fragments arranged by size:
4072 1449 943 633 230 111 82 8
BbeI G_GCGC'C
Cuts at: 2114 2142 2114
Size: 28 7500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4060 4094 5719 5753 1903
Size: 34 2123 34 1625 34 3678
Fragments arranged by size:
3678 2123 1625 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5420 6630 3845
Size: 1575 1210 4743
Fragments arranged by size:
4743 1575 1210
BclI T'GATC_A
Cuts at: 1548 3483 1548
Size: 1935 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4371 6139 136
Size: 107 122 71 631 605 2699 1768 1525
Fragments arranged by size:
2699 1768 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3432 7523 3432
Size: 4091 3437
BlpI GC'TnA_GC
Cuts at: 4945 4945
Size: 7528
Bme1580I G_kGCm'C
Cuts at: 1790 2603 2886 3057 5589 6745 1790
Size: 813 283 171 2532 1156 2573
Fragments arranged by size:
2573 2532 1156 813 283 171
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5259 6217 442
Size: 416 53 83 399 24 3842 958 1753
Fragments arranged by size:
3842 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 3605 2716
Size: 889 6639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2673 3371 3437 3751 3959 4388 4641 4670
Size: 1434 698 66 314 208 429 253 29
Cuts at: 4670 6188 1239
Size: 1518 2579
Fragments arranged by size:
2579 1518 1434 698 429 314 253 208
66 29
Bpu10I CC'TnA_GC
Cuts at: 2969 2969
Size: 7528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 5653 6518 6816 2558
Size: 3095 865 298 3270
Fragments arranged by size:
3270 3095 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3212 4028 4822 6191 882
Size: 617 1713 816 794 1369 2219
Fragments arranged by size:
2219 1713 1369 816 794 617
BsaAI yAC'GTr
Cuts at: 493 1204 5319 493
Size: 711 4115 2702
Fragments arranged by size:
4115 2702 711
BsaBI GATnn'nnATC
Cuts at: 4584 7522 4584
Size: 2938 4590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 2139 2403 4737
Size: 53 83 186 936 578 28 264 2334
Cuts at: 4737 5335 5717 6352 275
Size: 598 382 635 1451
Fragments arranged by size:
2334 1451 936 635 598 578 382 264
186 83 53 28
BsaWI w'CCGG_w
Cuts at: 2913 3255 3522 5961 6359 6633 6780 7191 2913
Size: 342 267 2439 398 274 147 411 3250
Fragments arranged by size:
3250 2439 411 398 342 274 267 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2804 2834 1178
Size: 30 165 30 1401 30 5872
Fragments arranged by size:
5872 1401 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2825 1186
Size: 1639 5889
BseYI C'CCAG_C
Cuts at: 2127 2156 3056 3187 3904 4590 6731 7227 2127
Size: 29 900 131 717 686 2141 496 2428
Fragments arranged by size:
2428 2141 900 717 686 496 131 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 4181 1163
Size: 573 744 1701 4510
Fragments arranged by size:
4510 1701 744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2150 3702 4978 5739 6767 1540
Size: 275 186 149 1552 1276 761 1028 2301
Fragments arranged by size:
2301 1552 1276 1028 761 275 186 149
BsiHKAI G_wGCw'C
Cuts at: 2024 2238 2886 2975 3945 4506 4544 5589 5674
Size: 214 648 89 970 561 38 1045 85
Cuts at: 5674 6745 2024
Size: 1071 2807
Fragments arranged by size:
2807 1071 1045 970 648 561 214 89
85 38
BsmI GAATG_Cn'
Cuts at: 5039 5132 5039
Size: 93 7435
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2238 2603 2886 2967 2975 3057
Size: 399 234 214 365 283 81 8 82
Cuts at: 3057 3945 4506 4544 4617 4847 5589 5674 6745
Size: 888 561 38 73 230 742 85 1071
Cuts at: 6745 1391
Size: 2174
Fragments arranged by size:
2174 1071 888 742 561 399 365 283
234 230 214 85 82 81 73 38
8
BspEI T'CCGG_A
Cuts at: 2913 3255 7191 2913
Size: 342 3936 3250
Fragments arranged by size:
3936 3250 342
BspHI T'CATG_A
Cuts at: 5417 7147 5417
Size: 1730 5798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2313 2869 4883 844
Size: 1469 556 2014 3489
Fragments arranged by size:
3489 2014 1469 556
BsrBI CCG'CTC
Cuts at: 1394 1823 2170 2194 5415 1394
Size: 429 347 24 3221 3507
Fragments arranged by size:
3507 3221 429 347 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 6023 6197 65
Size: 1183 1179 3596 174 1396
Fragments arranged by size:
3596 1396 1183 1179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3144 6172 6359 1700
Size: 110 1334 3028 187 2869
Fragments arranged by size:
3028 2869 1334 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7528
BssHII G'CGCG_C
Cuts at: 1925 2066 1925
Size: 141 7387
BssSI C'ACGA_G
Cuts at: 5588 6600 5588
Size: 1012 6516
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7528
BstBI TT'CG_AA
Cuts at: 6332 6332
Size: 7528
BstEII G'GTnAC_C
Cuts at: 1184 4333 4438 1184
Size: 3149 105 4274
Fragments arranged by size:
4274 3149 105
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 3415 2206
Size: 1209 6319
BstYI r'GATC_y
Cuts at: 1972 2265 3156 3383 3432 3626 4633 4858 5225
Size: 293 891 227 49 194 1007 225 367
Cuts at: 5225 5610 5627 7079 7165 7177 7523 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1007 891 385 367 346 293
227 225 194 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 2244 2934 3067 3120 3687
Size: 552 939 11 229 690 133 53 567
Cuts at: 3687 513
Size: 4354
Fragments arranged by size:
4354 939 690 567 552 229 133 53
11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3082 4376 487
Size: 683 56 1856 1294 3639
Fragments arranged by size:
3639 1856 1294 683 56
BtsI GCAGTG_nn'
Cuts at: 3107 3128 5044 5838 5858 3107
Size: 21 1916 794 20 4777
Fragments arranged by size:
4777 1916 794 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7493
DraI TTT'AAA
Cuts at: 4566 4832 4566
Size: 266 7262
DraIII CAC_nnn'GTG
Cuts at: 2325 2325
Size: 7528
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6535 809
Size: 507 5219 1802
Fragments arranged by size:
5219 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2147 2208 2253
Size: 54 1425 50 275 18 317 61 45
Cuts at: 2253 2761 3339 3489 4975 5864 7493 8
Size: 508 578 150 1486 889 1629 43
Fragments arranged by size:
1629 1486 1425 889 578 508 317 275
150 61 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2147 4975 1537
Size: 275 335 2828 4090
Fragments arranged by size:
4090 2828 335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3509 4428 5458 1353
Size: 2156 919 1030 3423
Fragments arranged by size:
3423 2156 1030 919
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3917 6115 6489 6635 387
Size: 1064 138 2328 2198 374 146 1280
Fragments arranged by size:
2328 2198 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2022 2973 4504 2022
Size: 951 1531 5046
Fragments arranged by size:
5046 1531 951
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 2673 2753 3371 3437 3751 3959
Size: 393 953 88 80 618 66 314 208
Cuts at: 3959 4388 4641 4670 5591 6188 6975 7243 1239
Size: 429 253 29 921 597 787 268 1524
Fragments arranged by size:
1524 953 921 787 618 597 429 393
314 268 253 208 88 80 66 29
EcoO109I rG'GnC_Cy
Cuts at: 2118 3053 3672 4372 2118
Size: 935 619 700 5274
Fragments arranged by size:
5274 935 700 619
EcoRI G'AATT_C
Cuts at: 4837 5215 4837
Size: 378 7150
EcoRV GAT'ATC
Cuts at: 3919 3919
Size: 7528
FspI TGC'GCA
Cuts at: 6034 6034
Size: 7528
HaeII r_GCGC'y
Cuts at: 1678 2114 2142 6675 1678
Size: 436 28 4533 2531
Fragments arranged by size:
4533 2531 436 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3535 3567 3618 3650 3903
Size: 32 135 32 1963 32 51 32 253
Cuts at: 3903 3935 4637 4669 4800 4832 6169 6201 6243
Size: 32 702 32 131 32 1337 32 42
Cuts at: 6243 6275 1373
Size: 32 2626
Fragments arranged by size:
2626 1963 1337 702 253 135 131 51
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4872 5118 669
Size: 988 281 2934 246 3079
Fragments arranged by size:
3079 2934 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7528
HpaI GTT'AAC
Cuts at: 5118 5118
Size: 7528
KasI G'GCGC_C
Cuts at: 2110 2138 2110
Size: 28 7500
KpnI G_GTAC'C
Cuts at: 4853 4853
Size: 7528
MfeI C'AATT_G
Cuts at: 5127 5127
Size: 7528
MluI A'CGCG_T
Cuts at: 7404 7404
Size: 7528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2727 6642 6826 1305
Size: 1422 3915 184 2007
Fragments arranged by size:
3915 2007 1422 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2255 2763 3491 10
Size: 54 1425 343 378 45 508 728 4047
Fragments arranged by size:
4047 1425 728 508 378 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2951 3542 3959 4082 4340 4788 5486
Size: 648 1785 591 417 123 258 448 698
Cuts at: 5486 5845 6004 518
Size: 359 159 2042
Fragments arranged by size:
2042 1785 698 648 591 448 417 359
258 159 123
MspA1I CmG'CkG
Cuts at: 1606 2017 2095 2907 3154 3424 4127 4450 4743
Size: 411 78 812 247 270 703 323 293
Cuts at: 4743 4944 5621 6769 7014 1606
Size: 201 677 1148 245 2120
Fragments arranged by size:
2120 1148 812 703 677 411 323 293
270 247 245 201 78
NaeI GCC'GGC
Cuts at: 3146 3146
Size: 7528
NarI GG'CG_CC
Cuts at: 2111 2139 2111
Size: 28 7500
NcoI C'CATG_G
Cuts at: 513 1065 2004 2934 3120 513
Size: 552 939 930 186 4921
Fragments arranged by size:
4921 939 930 552 186
NdeI CA'TA_TG
Cuts at: 387 3960 387
Size: 3573 3955
NgoMIV G'CCGG_C
Cuts at: 3144 3144
Size: 7528
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7528
NotI GC'GGCC_GC
Cuts at: 4975 4975
Size: 7528
NspI r_CATG'y
Cuts at: 1132 1229 2821 3137 3817 4629 4793 4886 6431
Size: 97 1592 316 680 812 164 93 1545
Cuts at: 6431 1132
Size: 2229
Fragments arranged by size:
2229 1592 1545 812 680 316 164 97
93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7528
PciI A'CATG_T
Cuts at: 2817 4789 6427 2817
Size: 1972 1638 3918
Fragments arranged by size:
3918 1972 1638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 2779 3187 1108
Size: 1281 390 408 5449
Fragments arranged by size:
5449 1281 408 390
PfoI T'CCnGG_A
Cuts at: 3159 3414 3936 3159
Size: 255 522 6751
Fragments arranged by size:
6751 522 255
PmeI GTTT'AAAC
Cuts at: 4832 4832
Size: 7528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5597 5629 7110 7142 5597
Size: 32 1481 32 5983
Fragments arranged by size:
5983 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3672 3672
Size: 7528
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7528
PsiI TTA'TAA
Cuts at: 5098 5098
Size: 7528
PspOMI G'GGCC_C
Cuts at: 3053 3053
Size: 7528
PstI C_TGCA'G
Cuts at: 830 3114 4199 4455 4880 830
Size: 2284 1085 256 425 3478
Fragments arranged by size:
3478 2284 1085 425 256
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7528
PvuII CAG'CTG
Cuts at: 3424 4450 4743 3424
Size: 1026 293 6209
Fragments arranged by size:
6209 1026 293
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 7528
SacI G_AGCT'C
Cuts at: 2024 2975 4506 2024
Size: 951 1531 5046
Fragments arranged by size:
5046 1531 951
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 7528
SalI G'TCGA_C
Cuts at: 1936 4870 1936
Size: 2934 4594
SbfI CC_TGCA'GG
Cuts at: 4880 4880
Size: 7528
ScaI AGT'ACT
Cuts at: 5776 7488 5776
Size: 1712 5816
SfcI C'TryA_G
Cuts at: 826 1045 3110 3448 4195 4376 4451 4595 4638
Size: 219 2065 338 747 181 75 144 43
Cuts at: 4638 4876 6011 6692 6883 826
Size: 238 1135 681 191 1471
Fragments arranged by size:
2065 1471 1135 747 681 338 238 219
191 181 144 75 43
SfoI GGC'GCC
Cuts at: 2112 2140 2112
Size: 28 7500
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7528
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7528
SmaI CCC'GGG
Cuts at: 1872 2035 2116 4145 4855 1872
Size: 163 81 2029 710 4545
Fragments arranged by size:
4545 2029 710 163 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 5632 6533 6795 820
Size: 197 926 630 3059 901 262 1553
Fragments arranged by size:
3059 1553 926 901 630 262 197
SnaBI TAC'GTA
Cuts at: 493 5319 493
Size: 4826 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7528
SphI G_CATG'C
Cuts at: 1132 3137 3817 4886 1132
Size: 2005 680 1069 3774
Fragments arranged by size:
3774 2005 1069 680
SrfI GCCC'GGGC
Cuts at: 2035 2035
Size: 7528
SspI AAT'ATT
Cuts at: 5 52 5452 5
Size: 47 5400 2081
Fragments arranged by size:
5400 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7528
StyI C'CwwG_G
Cuts at: 513 1065 2004 2199 2211 2443 2758 2934 3120
Size: 552 939 195 12 232 315 176 186
Cuts at: 3120 3379 3577 4967 513
Size: 259 198 1390 3074
Fragments arranged by size:
3074 1390 939 552 315 259 232 198
195 186 176 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3971 4321 5555 5572 5725 5910 6355
Size: 1255 2391 350 1234 17 153 185 445
Cuts at: 6355 325
Size: 1498
Fragments arranged by size:
2391 1498 1255 1234 445 350 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3027 3327 3885
Size: 274 80 33 51 1443 1050 300 558
Cuts at: 3885 4074 5774 7486 96
Size: 189 1700 1712 138
Fragments arranged by size:
1712 1700 1443 1050 558 300 274 189
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4788 4820 7488 7520 4788
Size: 32 2668 32 4796
Fragments arranged by size:
4796 2668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7528
XbaI T'CTAG_A
Cuts at: 4864 4864
Size: 7528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4729 4729
Size: 7528
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7528
XmaI C'CCGG_G
Cuts at: 1870 2033 2114 4143 4853 1870
Size: 163 81 2029 710 4545
Fragments arranged by size:
4545 2029 710 163 81
XmnI GAAnn'nnTTC
Cuts at: 2351 5657 2351
Size: 3306 4222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5336 276
Size: 53 83 186 936 3802 2468
Fragments arranged by size:
3802 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoNI FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI SanDI SapI SexAI SfiI
SwaI