Restriction map for
pFN21AB3072
(Circular) (Six-base) MAPSORT of: pFN21AB3072.seq Check: 6104 from: 1 to: 6571
pFN21AB3072 6571 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3512 3743 3512
Size: 231 6340
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3914 1937
Size: 1977 4594
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2633 2963 4634 6018 6286 1632
Size: 1001 330 1671 1384 268 1917
Fragments arranged by size:
1917 1671 1384 1001 330 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 3801 5402 3801
Size: 1601 4970
AhdI GACnn_n'nnGTC
Cuts at: 3001 3564 5300 3001
Size: 563 1736 4272
Fragments arranged by size:
4272 1736 563
AleI CACnn'nnGTG
Cuts at: 1166 2060 1166
Size: 894 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2975 3542 1184
Size: 1791 567 4213
Fragments arranged by size:
4213 1791 567
AlwNI CAG_nnn'CTG
Cuts at: 2129 2370 2461 2503 2617 2800 2881 3085 3457
Size: 241 91 42 114 183 81 204 372
Cuts at: 3457 3676 4314 5886 2129
Size: 219 638 1572 2814
Fragments arranged by size:
2814 1572 638 372 241 219 204 183
114 91 81 42
ApaLI G'TGCA_C
Cuts at: 3194 4628 5784 3194
Size: 1434 1156 3981
Fragments arranged by size:
3981 1434 1156
ApoI r'AATT_y
Cuts at: 1493 2206 2323 3880 4106 4258 6367 1493
Size: 713 117 1557 226 152 2109 1697
Fragments arranged by size:
2109 1697 1557 713 226 152 117
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2160 2909 3013 3251 3645 3896 1870
Size: 73 217 749 104 238 394 251 4545
Fragments arranged by size:
4545 749 394 251 238 217 104 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2338 3892 5347 618
Size: 325 217 627 267 284 1554 1455 1842
Fragments arranged by size:
1842 1554 1455 627 325 284 267 217
BanII G_rGCy'C
Cuts at: 1391 2021 2123 2219 2454 2871 3626 3890 1391
Size: 630 102 96 235 417 755 264 4072
Fragments arranged by size:
4072 755 630 417 264 235 102 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5600
BbvCI CC'TCA_GC
Cuts at: 2142 2142
Size: 6571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3490 3524 4762 4796 1903
Size: 34 1553 34 1238 34 3678
Fragments arranged by size:
3678 1553 1238 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3477 4463 5673 2796
Size: 681 986 1210 3694
Fragments arranged by size:
3694 1210 986 681
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 2982 5182
Size: 107 122 71 631 605 364 946 2200
Cuts at: 5182 136
Size: 1525
Fragments arranged by size:
2200 1525 946 631 605 364 122 107
71
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 2122 3218 3443 3988 2122
Size: 1096 225 545 4705
Fragments arranged by size:
4705 1096 545 225
Bme1580I G_kGCm'C
Cuts at: 1790 2964 3198 3764 4632 5788 1790
Size: 1174 234 566 868 1156 2573
Fragments arranged by size:
2573 1174 1156 868 566 234
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6571
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2813 4302 5260
Size: 416 53 83 399 24 1396 1489 958
Cuts at: 5260 442
Size: 1753
Fragments arranged by size:
1753 1489 1396 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 3039 2305
Size: 734 5837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2169 2178 2220 2438 2911 3200 3224 3343
Size: 930 9 42 218 473 289 24 119
Cuts at: 3343 3479 5231 1239
Size: 136 1752 2579
Fragments arranged by size:
2579 1752 930 473 289 218 136 119
42 24 9
Bpu10I CC'TnA_GC
Cuts at: 2142 2142
Size: 6571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4696 5561 5859 4696
Size: 865 298 5408
Fragments arranged by size:
5408 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2145 2260 5234 882
Size: 617 646 115 2974 2219
Fragments arranged by size:
2974 2219 646 617 115
BsaAI yAC'GTr
Cuts at: 493 1204 3589 4362 493
Size: 711 2385 773 2702
Fragments arranged by size:
2702 2385 773 711
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 4378 4760 5395
Size: 53 83 186 936 2032 813 382 635
Cuts at: 5395 275
Size: 1451
Fragments arranged by size:
2032 1451 936 813 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3801 5004 5402 5676 5823 6234 3801
Size: 1203 398 274 147 411 4138
Fragments arranged by size:
4138 1203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6346
Fragments arranged by size:
6346 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2099 3616 3764 1186
Size: 843 70 1517 148 3993
Fragments arranged by size:
3993 1517 843 148 70
BseYI C'CCAG_C
Cuts at: 2031 2155 2692 2739 2874 2972 3163 5774 6270
Size: 124 537 47 135 98 191 2611 496
Cuts at: 6270 2031
Size: 2332
Fragments arranged by size:
2611 2332 537 496 191 135 124 98
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 2786 3071 3635 1163
Size: 573 317 733 285 564 4099
Fragments arranged by size:
4099 733 573 564 317 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4021 4782 5810 1540
Size: 275 186 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2454 3198 3626 3687 3864 4632 4717 5788 2454
Size: 744 428 61 177 768 85 1071 3237
Fragments arranged by size:
3237 1071 768 744 428 177 85 61
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2123 2219 2454 2871 2964 3198
Size: 399 231 102 96 235 417 93 234
Cuts at: 3198 3626 3687 3764 3864 3890 4632 4717 5788
Size: 428 61 77 100 26 742 85 1071
Cuts at: 5788 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 428 417 399 235 234
231 102 100 96 93 85 77 61
26
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 2466 2685 4460 6190 2466
Size: 219 1775 1730 2847
Fragments arranged by size:
2847 1775 1730 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2656 3006 3512 3743 3926 844
Size: 1812 350 506 231 183 3489
Fragments arranged by size:
3489 1812 506 350 231 183
BsrBI CCG'CTC
Cuts at: 1394 1823 3175 3319 4458 1394
Size: 429 1352 144 1139 3507
Fragments arranged by size:
3507 1352 1139 429 144
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3447 3801 5215 5402 1700
Size: 110 1637 354 1414 187 2869
Fragments arranged by size:
2869 1637 1414 354 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 2105 4631 5643 2105
Size: 2526 1012 3033
Fragments arranged by size:
3033 2526 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2800 3085 794
Size: 2006 285 4280
Fragments arranged by size:
4280 2006 285
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6571
BstYI r'GATC_y
Cuts at: 1972 2658 3327 3372 3380 3901 4268 4653 4670
Size: 686 669 45 8 521 367 385 17
Cuts at: 4670 6122 6208 6220 6566 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 686 669 521 385 367 346
86 45 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2058 2841 2977 3488 513
Size: 552 993 783 136 511 3596
Fragments arranged by size:
3596 993 783 552 511 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3858 487
Size: 683 56 2632 3200
Fragments arranged by size:
3200 2632 683 56
BtsI GCAGTG_nn'
Cuts at: 2127 4087 4881 4901 2127
Size: 1960 794 20 3797
Fragments arranged by size:
3797 1960 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DraIII CAC_nnn'GTG
Cuts at: 3781 3781
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2844 3607 4018
Size: 54 1425 50 275 18 1014 763 411
Cuts at: 4018 4907 6536 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1014 889 763 411 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 3828 3833 4501 1353
Size: 1469 1006 5 668 3423
Fragments arranged by size:
3423 1469 1006 668 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5158 5532 5678 387
Size: 1064 138 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2452 3624 2452
Size: 1172 5399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 2178 2220 2438 2633 2911 2963
Size: 393 537 9 42 218 195 278 52
Cuts at: 2963 3200 3224 3343 3479 4634 5231 6018 6286
Size: 237 24 119 136 1155 597 787 268
Cuts at: 6286 1239
Size: 1524
Fragments arranged by size:
1524 1155 787 597 537 393 278 268
237 218 195 136 119 52 42 24
9
EcoNI CCTnn'n_nnAGG
Cuts at: 3746 3746
Size: 6571
EcoO109I rG'GnC_Cy
Cuts at: 2110 2250 2424 2905 2984 2995 3102 3139 3547
Size: 140 174 481 79 11 107 37 408
Cuts at: 3547 3558 2110
Size: 11 5123
Fragments arranged by size:
5123 481 408 174 140 107 79 37
11 11
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 6571
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 5718 1678
Size: 4040 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 2987 3019 3550
Size: 32 135 32 644 32 739 32 531
Cuts at: 3550 3582 3761 3793 3806 3838 5212 5244 5286
Size: 32 179 32 13 32 1374 32 42
Cuts at: 5286 5318 1373
Size: 32 2626
Fragments arranged by size:
2626 1374 739 644 531 179 135 42
32 32 32 32 32 32 32 32
32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2671 3730 3915 4161 669
Size: 988 281 733 1059 185 246 3079
Fragments arranged by size:
3079 1059 988 733 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 2671 4161 2671
Size: 1490 5081
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 5685 5869 1305
Size: 954 3426 184 2007
Fragments arranged by size:
3426 2007 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2846 3609 10
Size: 54 1425 343 1014 763 2972
Fragments arranged by size:
2972 1425 1014 763 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2060 3719 4529 4888 5047 518
Size: 648 843 51 1659 810 359 159 2042
Fragments arranged by size:
2042 1659 843 810 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2035 2135 2479 2617 2743 2791 2797 2955
Size: 429 100 344 138 126 48 6 158
Cuts at: 2955 3082 3490 3664 3987 4664 5812 6057 1606
Size: 127 408 174 323 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 429 408 344 323 245
174 158 138 127 126 100 48 6
NaeI GCC'GGC
Cuts at: 3449 3449
Size: 6571
NcoI C'CATG_G
Cuts at: 513 1065 2058 2841 2977 513
Size: 552 993 783 136 4107
Fragments arranged by size:
4107 993 783 552 136
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NgoMIV G'CCGG_C
Cuts at: 3447 3447
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 2677 3352 3661 3718 3929 5474 1132
Size: 97 1448 675 309 57 211 1545 2229
Fragments arranged by size:
2229 1545 1448 675 309 211 97 57
PasI CC'CwG_GG
Cuts at: 1348 2116 2200 1348
Size: 768 84 5719
Fragments arranged by size:
5719 768 84
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2370 2461 1108
Size: 1262 91 5218
Fragments arranged by size:
5218 1262 91
PfoI T'CCnGG_A
Cuts at: 3066 3066
Size: 6571
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2250 2424 2995 3139 3558 2250
Size: 174 571 144 419 5263
Fragments arranged by size:
5263 571 419 174 144
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PstI C_TGCA'G
Cuts at: 830 2796 2865 3003 3740 3923 830
Size: 1966 69 138 737 183 3478
Fragments arranged by size:
3478 1966 737 183 138 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2035 2479 2617 2743 2797 2955 3082 2035
Size: 444 138 126 54 158 127 5524
Fragments arranged by size:
5524 444 158 138 127 126 54
SacI G_AGCT'C
Cuts at: 2454 3626 2454
Size: 1172 5399
SacII CC_GC'GG
Cuts at: 3491 3491
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SanDI GG'GwC_CC
Cuts at: 2250 2424 2250
Size: 174 6397
SapI GCTCTTCn'nnn_
Cuts at: 3833 3833
Size: 6571
SbfI CC_TGCA'GG
Cuts at: 2865 3923 2865
Size: 1058 5513
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SfcI C'TryA_G
Cuts at: 826 1045 2227 2792 2861 2999 3736 3919 5054
Size: 219 1182 565 69 138 737 183 1135
Cuts at: 5054 5735 5926 826
Size: 681 191 1471
Fragments arranged by size:
1471 1182 1135 737 681 565 219 191
183 138 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 2162 3898 1872
Size: 290 1736 4545
Fragments arranged by size:
4545 1736 290
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4675 5576 5838 820
Size: 197 926 2732 901 262 1553
Fragments arranged by size:
2732 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3661 3929 1132
Size: 2529 268 3774
Fragments arranged by size:
3774 2529 268
SrfI GCCC'GGGC
Cuts at: 2162 2162
Size: 6571
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 2833 1517
Size: 1316 5255
StyI C'CwwG_G
Cuts at: 513 1065 2058 2841 2901 2977 3034 3105 3168
Size: 552 993 783 60 76 57 71 63
Cuts at: 3168 3309 3376 4010 513
Size: 141 67 634 3074
Fragments arranged by size:
3074 993 783 634 552 141 76 71
67 63 60 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2864 3213 4598 4615 4768 4953 5398
Size: 1255 1284 349 1385 17 153 185 445
Cuts at: 5398 325
Size: 1498
Fragments arranged by size:
1498 1385 1284 1255 445 349 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3480 3540 4817
Size: 274 80 33 51 1443 1503 60 1277
Cuts at: 4817 6529 96
Size: 1712 138
Fragments arranged by size:
1712 1503 1443 1277 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 6531 6563 2465
Size: 32 4034 32 2473
Fragments arranged by size:
4034 2473 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3050 1316
Size: 1734 4837
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3175 3175
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6571
XmaI C'CCGG_G
Cuts at: 1870 2160 3896 1870
Size: 290 1736 4545
Fragments arranged by size:
4545 1736 290
XmnI GAAnn'nnTTC
Cuts at: 3293 4700 3293
Size: 1407 5164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SexAI SfiI SfoI SwaI