Restriction map for
pFN21AB3063
(Circular) (Six-base) MAPSORT of: pFN21AB3063.seq Check: 2008 from: 1 to: 6850
pFN21AB3063 6850 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4660 278
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2934 4193 1937
Size: 997 1259 4594
Fragments arranged by size:
4594 1259 997
Acc65I G'GTAC_C
Cuts at: 4171 4171
Size: 6850
AclI AA'CG_TT
Cuts at: 1563 2306 4977 5350 1563
Size: 743 2671 373 3063
Fragments arranged by size:
3063 2671 743 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 2556 2957 3575 4913 6297 6565 1632
Size: 599 325 401 618 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 618 599 401 325 268
AfeI AGC'GCT
Cuts at: 3075 3243 3075
Size: 168 6682
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6653
AflIII A'CryG_T
Cuts at: 1203 2090 3666 5749 6726 1203
Size: 887 1576 2083 977 1327
Fragments arranged by size:
2083 1576 1327 977 887
AgeI A'CCGG_T
Cuts at: 5681 5681
Size: 6850
AhdI GACnn_n'nnGTC
Cuts at: 5579 5579
Size: 6850
AleI CACnn'nnGTG
Cuts at: 1166 4022 1166
Size: 2856 3994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6850
AlwNI CAG_nnn'CTG
Cuts at: 2636 4593 6165 2636
Size: 1957 1572 3321
Fragments arranged by size:
3321 1957 1572
ApaLI G'TGCA_C
Cuts at: 2859 4907 6063 2859
Size: 2048 1156 3646
Fragments arranged by size:
3646 2048 1156
ApoI r'AATT_y
Cuts at: 1493 2135 2263 2823 3914 4159 4385 4537 6646
Size: 642 128 560 1091 245 226 152 2109
Cuts at: 6646 1493
Size: 1697
Fragments arranged by size:
2109 1697 1091 642 560 245 226 152
128
AseI AT'TA_AT
Cuts at: 160 1032 2057 2328 5404 160
Size: 872 1025 271 3076 1606
Fragments arranged by size:
3076 1606 1025 872 271
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 3077 3954 4175 1870
Size: 73 325 809 877 221 4545
Fragments arranged by size:
4545 877 809 325 221 73
BamHI G'GATC_C
Cuts at: 4180 4547 4180
Size: 367 6483
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2702 3313 4171 5626 618
Size: 325 217 627 915 611 858 1455 1842
Fragments arranged by size:
1842 1455 915 858 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2694 3607 3748 4169 1391
Size: 1303 913 141 421 4072
Fragments arranged by size:
4072 1303 913 421 141
BbeI G_GCGC'C
Cuts at: 3317 3317
Size: 6850
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3555 3586 928
Size: 971 1656 31 4192
Fragments arranged by size:
4192 1656 971 31
BbvCI CC'TCA_GC
Cuts at: 2669 2669
Size: 6850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2828 2862 3953 3987 5041 5075 1903
Size: 34 891 34 1091 34 1054 34 3678
Fragments arranged by size:
3678 1091 1054 891 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4742 5952 4742
Size: 1210 5640
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6850
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4080 5461 136
Size: 107 122 71 631 605 2408 1381 1525
Fragments arranged by size:
2408 1525 1381 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6845 6845
Size: 6850
BlpI GC'TnA_GC
Cuts at: 4267 4267
Size: 6850
Bme1580I G_kGCm'C
Cuts at: 1790 2541 2705 2863 4911 6067 1790
Size: 751 164 158 2048 1156 2573
Fragments arranged by size:
2573 2048 1156 751 164 158
BmgBI CAC'GTC
Cuts at: 1318 2913 3667 3967 1318
Size: 1595 754 300 4201
Fragments arranged by size:
4201 1595 754 300
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2021 3299 4581
Size: 416 53 83 399 24 604 1278 1282
Cuts at: 4581 5539 442
Size: 958 1753
Fragments arranged by size:
1753 1282 1278 958 604 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2786 3438 3560 4085 4099 4114 4129 5510
Size: 1547 652 122 525 14 15 15 1381
Cuts at: 5510 1239
Size: 2579
Fragments arranged by size:
2579 1547 1381 652 525 122 15 15
14
Bpu10I CC'TnA_GC
Cuts at: 2669 2688 3035 3128 2669
Size: 19 347 93 6391
Fragments arranged by size:
6391 347 93 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 4975 5840 6138 2627
Size: 2348 865 298 3339
Fragments arranged by size:
3339 2348 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2801 3220 3563 3991 5513 882
Size: 617 1302 419 343 428 1522 2219
Fragments arranged by size:
2219 1522 1302 617 428 419 343
BsaAI yAC'GTr
Cuts at: 493 1204 3031 4641 493
Size: 711 1827 1610 2702
Fragments arranged by size:
2702 1827 1610 711
BsaBI GATnn'nnATC
Cuts at: 6844 6844
Size: 6850
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3314 4657 5039 5674
Size: 53 83 186 936 1781 1343 382 635
Cuts at: 5674 275
Size: 1451
Fragments arranged by size:
1781 1451 1343 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3522 5283 5681 5955 6102 6513 3522
Size: 1761 398 274 147 411 3859
Fragments arranged by size:
3859 1761 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3092 3122 3217 3247 3464
Size: 30 165 30 1689 30 95 30 217
Cuts at: 3464 3494 3846 3876 4118 4148 1178
Size: 30 352 30 242 30 3880
Fragments arranged by size:
3880 1689 352 242 217 165 95 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2952 3548 4102 1186
Size: 1766 596 554 3934
Fragments arranged by size:
3934 1766 596 554
BseYI C'CCAG_C
Cuts at: 2678 3040 3805 4089 6053 6549 2678
Size: 362 765 284 1964 496 2979
Fragments arranged by size:
2979 1964 765 496 362 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 3800 3884 1163
Size: 573 294 1770 84 4129
Fragments arranged by size:
4129 1770 573 294 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4300 5061 6089 1540
Size: 275 186 2299 761 1028 2301
Fragments arranged by size:
2301 2299 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2863 3036 3336 4911 4996 6067 2863
Size: 173 300 1575 85 1071 3646
Fragments arranged by size:
3646 1575 1071 300 173 85
BsmI GAATG_Cn'
Cuts at: 4361 4454 4361
Size: 93 6757
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2541 2694 2705 2863 3036 3336 3607
Size: 399 751 153 11 158 173 300 271
Cuts at: 3607 3748 4169 4911 4996 6067 1391
Size: 141 421 742 85 1071 2174
Fragments arranged by size:
2174 1071 751 742 421 399 300 271
173 158 153 141 85 11
BspEI T'CCGG_A
Cuts at: 6513 6513
Size: 6850
BspHI T'CATG_A
Cuts at: 4739 6469 4739
Size: 1730 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 3973 4205 844
Size: 2347 782 232 3489
Fragments arranged by size:
3489 2347 782 232
BsrBI CCG'CTC
Cuts at: 1394 1823 4737 1394
Size: 429 2914 3507
Fragments arranged by size:
3507 2914 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2335 2454 5345 5519 65
Size: 1183 1087 119 2891 174 1396
Fragments arranged by size:
2891 1396 1183 1087 174 119
BsrFI r'CCGG_y
Cuts at: 1700 1810 5494 5681 1700
Size: 110 3684 187 2869
Fragments arranged by size:
3684 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2037 96
Size: 1941 4909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6850
BssSI C'ACGA_G
Cuts at: 4910 5922 4910
Size: 1012 5838
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6850
BstBI TT'CG_AA
Cuts at: 5654 5654
Size: 6850
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6850
BstXI CCAn_nnnn'nTGG
Cuts at: 3064 3416 3064
Size: 352 6498
BstYI r'GATC_y
Cuts at: 1972 4180 4547 4932 4949 6401 6487 6499 6845
Size: 2208 367 385 17 1452 86 12 346
Cuts at: 6845 1972
Size: 1977
Fragments arranged by size:
2208 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2964 2964
Size: 6850
BtgI C'CryG_G
Cuts at: 513 1065 2004 2613 2694 513
Size: 552 939 609 81 4669
Fragments arranged by size:
4669 939 609 552 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3148 487
Size: 683 56 1922 4189
Fragments arranged by size:
4189 1922 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 3914 4366 5160 5180 2125
Size: 1789 452 794 20 3795
Fragments arranged by size:
3795 1789 794 452 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6815
DraI TTT'AAA
Cuts at: 4154 4154
Size: 6850
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5857 809
Size: 507 4541 1802
Fragments arranged by size:
4541 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2616 3887 4297
Size: 54 1425 50 275 18 786 1271 410
Cuts at: 4297 5186 6815 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1271 889 786 410 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4297 1537
Size: 275 2485 4090
Fragments arranged by size:
4090 2485 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2575 3135 3994 4780 1353
Size: 1222 560 859 786 3423
Fragments arranged by size:
3423 1222 859 786 560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2541 5437 5811 5957 387
Size: 1064 138 952 2896 374 146 1280
Fragments arranged by size:
2896 1280 1064 952 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2556 2786 2957 3438 3560 3575
Size: 393 599 325 230 171 481 122 15
Cuts at: 3575 4085 4099 4114 4129 4913 5510 6297 6565
Size: 510 14 15 15 784 597 787 268
Cuts at: 6565 1239
Size: 1524
Fragments arranged by size:
1524 787 784 599 597 510 481 393
325 268 230 171 122 15 15 15
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2074 2645 2074
Size: 571 6279
EcoO109I rG'GnC_Cy
Cuts at: 2968 3413 2968
Size: 445 6405
EcoRI G'AATT_C
Cuts at: 2135 2263 4159 4537 2135
Size: 128 1896 378 4448
Fragments arranged by size:
4448 1896 378 128
FspI TGC'GCA
Cuts at: 5356 5356
Size: 6850
HaeII r_GCGC'y
Cuts at: 1678 2179 3077 3245 3317 5997 1678
Size: 501 898 168 72 2680 2531
Fragments arranged by size:
2680 2531 898 501 168 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 2884 2916 2947
Size: 32 135 32 821 32 459 32 31
Cuts at: 2947 2979 3090 3122 3626 3658 5491 5523 5565
Size: 32 111 32 504 32 1833 32 42
Cuts at: 5565 5597 1373
Size: 32 2626
Fragments arranged by size:
2626 1833 821 504 459 135 111 42
32 32 32 32 32 32 32 32
32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 4194 4440 669
Size: 988 281 2256 246 3079
Fragments arranged by size:
3079 2256 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6850
HpaI GTT'AAC
Cuts at: 4440 4440
Size: 6850
KasI G'GCGC_C
Cuts at: 3313 3313
Size: 6850
KpnI G_GTAC'C
Cuts at: 4175 4175
Size: 6850
MfeI C'AATT_G
Cuts at: 4449 4449
Size: 6850
MluI A'CGCG_T
Cuts at: 6726 6726
Size: 6850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2525 5964 6148 1305
Size: 1220 3439 184 2007
Fragments arranged by size:
3439 2007 1220 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5028
Fragments arranged by size:
5028 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3260 4022 4808 5167 5326 518
Size: 648 2094 762 786 359 159 2042
Fragments arranged by size:
2094 2042 786 762 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2764 3805 4080 4093 4266 4943 6091 6336
Size: 1158 1041 275 13 173 677 1148 245
Cuts at: 6336 1606
Size: 2120
Fragments arranged by size:
2120 1158 1148 1041 677 275 245 173
13
NarI GG'CG_CC
Cuts at: 3314 3314
Size: 6850
NcoI C'CATG_G
Cuts at: 513 1065 2004 2613 513
Size: 552 939 609 4750
Fragments arranged by size:
4750 939 609 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6850
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6850
NotI GC'GGCC_GC
Cuts at: 4297 4297
Size: 6850
NspI r_CATG'y
Cuts at: 1132 1229 2094 3214 3481 3928 4208 5753 1132
Size: 97 865 1120 267 447 280 1545 2229
Fragments arranged by size:
2229 1545 1120 865 447 280 267 97
PasI CC'CwG_GG
Cuts at: 1348 3318 3739 1348
Size: 1970 421 4459
Fragments arranged by size:
4459 1970 421
PciI A'CATG_T
Cuts at: 2090 5749 2090
Size: 3659 3191
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3497 1108
Size: 2389 4461
PfoI T'CCnGG_A
Cuts at: 2802 3415 2802
Size: 613 6237
PmeI GTTT'AAAC
Cuts at: 4154 4154
Size: 6850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 6432 6464 4919
Size: 32 1481 32 5305
Fragments arranged by size:
5305 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2968 3413 2968
Size: 445 6405
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6850
PsiI TTA'TAA
Cuts at: 2298 4420 2298
Size: 2122 4728
PstI C_TGCA'G
Cuts at: 830 2769 3532 3909 3984 4202 830
Size: 1939 763 377 75 218 3478
Fragments arranged by size:
3478 1939 763 377 218 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6850
PvuII CAG'CTG
Cuts at: 3805 4093 3805
Size: 288 6562
SalI G'TCGA_C
Cuts at: 1936 4192 1936
Size: 2256 4594
SbfI CC_TGCA'GG
Cuts at: 3984 4202 3984
Size: 218 6632
ScaI AGT'ACT
Cuts at: 5098 6810 5098
Size: 1712 5138
SexAI A'CCwGG_T
Cuts at: 3229 3881 3229
Size: 652 6198
SfcI C'TryA_G
Cuts at: 826 1045 2765 3528 3817 3905 3980 4198 5333
Size: 219 1720 763 289 88 75 218 1135
Cuts at: 5333 6014 6205 826
Size: 681 191 1471
Fragments arranged by size:
1720 1471 1135 763 681 289 219 218
191 88 75
SfoI GGC'GCC
Cuts at: 3315 3315
Size: 6850
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6850
SmaI CCC'GGG
Cuts at: 1872 3956 4177 1872
Size: 2084 221 4545
Fragments arranged by size:
4545 2084 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 4954 5855 6117 820
Size: 197 926 699 2312 901 262 1553
Fragments arranged by size:
2312 1553 926 901 699 262 197
SnaBI TAC'GTA
Cuts at: 493 4641 493
Size: 4148 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6850
SphI G_CATG'C
Cuts at: 1132 3928 4208 1132
Size: 2796 280 3774
Fragments arranged by size:
3774 2796 280
SspI AAT'ATT
Cuts at: 5 52 2875 4774 5
Size: 47 2823 1899 2081
Fragments arranged by size:
2823 2081 1899 47
StuI AGG'CCT
Cuts at: 1517 3423 1517
Size: 1906 4944
StyI C'CwwG_G
Cuts at: 513 1065 2004 2613 4289 513
Size: 552 939 609 1676 3074
Fragments arranged by size:
3074 1676 939 609 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2215 3739 4877 4894 5047 5232 5677
Size: 1255 635 1524 1138 17 153 185 445
Cuts at: 5677 325
Size: 1498
Fragments arranged by size:
1524 1498 1255 1138 635 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2037 2429 4011
Size: 274 80 33 51 1443 60 392 1582
Cuts at: 4011 5096 6808 96
Size: 1085 1712 138
Fragments arranged by size:
1712 1582 1443 1085 392 274 138 80
60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3217 3249 3464 3496 3846 3878 4118 4150 6810
Size: 32 215 32 350 32 240 32 2660
Cuts at: 6810 6842 3217
Size: 32 3225
Fragments arranged by size:
3225 2660 350 240 215 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3794 1316
Size: 2478 4372
XbaI T'CTAG_A
Cuts at: 4186 4186
Size: 6850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4060 4060
Size: 6850
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6850
XmaI C'CCGG_G
Cuts at: 1870 3954 4175 1870
Size: 2084 221 4545
Fragments arranged by size:
4545 2084 221
XmnI GAAnn'nnTTC
Cuts at: 2135 4979 2135
Size: 2844 4006
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4658 276
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI