Restriction map for
pFN21AB3051
(Circular) (Six-base) MAPSORT of: pFN21AB3051.seq Check: 4825 from: 1 to: 8350
pFN21AB3051 8350 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3414 4312 3414
Size: 898 7452
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2328 2696 6160 278
Size: 53 83 186 936 792 368 3464 2468
Fragments arranged by size:
3464 2468 936 792 368 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3156 4631 5577 5693 1937
Size: 1219 1475 946 116 4594
Fragments arranged by size:
4594 1475 1219 946 116
Acc65I G'GTAC_C
Cuts at: 3170 4080 5671 3170
Size: 910 1591 5849
Fragments arranged by size:
5849 1591 910
AclI AA'CG_TT
Cuts at: 1563 6477 6850 1563
Size: 4914 373 3063
Fragments arranged by size:
4914 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2165 2549 2588 3017 3743 5287 5299 6413
Size: 533 384 39 429 726 1544 12 1114
Cuts at: 6413 7797 8065 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1544 1384 1114 726 533 429 384
268 39 12
AfeI AGC'GCT
Cuts at: 3796 3796
Size: 8350
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8153
AflIII A'CryG_T
Cuts at: 1203 7249 8226 1203
Size: 6046 977 1327
Fragments arranged by size:
6046 1327 977
AgeI A'CCGG_T
Cuts at: 7181 7181
Size: 8350
AhdI GACnn_n'nnGTC
Cuts at: 3907 7079 3907
Size: 3172 5178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8350
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 8318
AlwNI CAG_nnn'CTG
Cuts at: 2143 3049 4213 6093 7665 2143
Size: 906 1164 1880 1572 2828
Fragments arranged by size:
2828 1880 1572 1164 906
ApaI G_GGCC'C
Cuts at: 3085 3085
Size: 8350
ApaLI G'TGCA_C
Cuts at: 3399 6407 7563 3399
Size: 3008 1156 4186
Fragments arranged by size:
4186 3008 1156
ApoI r'AATT_y
Cuts at: 1493 4972 5659 5885 6037 8146 1493
Size: 3479 687 226 152 2109 1697
Fragments arranged by size:
3479 2109 1697 687 226 152
AscI GG'CGCG_CC
Cuts at: 4917 4917
Size: 8350
AseI AT'TA_AT
Cuts at: 160 1032 6904 160
Size: 872 5872 1606
Fragments arranged by size:
5872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
AvaI C'yCGr_G
Cuts at: 1870 1943 2537 2594 4093 5675 1870
Size: 73 594 57 1499 1582 4545
Fragments arranged by size:
4545 1582 1499 594 73 57
BamHI G'GATC_C
Cuts at: 5680 6047 5680
Size: 367 7983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2318 3170 4005 4080 4200
Size: 325 217 627 531 852 835 75 120
Cuts at: 4200 5671 7126 618
Size: 1471 1455 1842
Fragments arranged by size:
1842 1471 1455 852 835 627 531 325
217 120 75
BanII G_rGCy'C
Cuts at: 1391 2790 3085 4061 4408 4692 5088 5135 5402
Size: 1399 295 976 347 284 396 47 267
Cuts at: 5402 5669 1391
Size: 267 4072
Fragments arranged by size:
4072 1399 976 396 347 295 284 267
267 47
BbeI G_GCGC'C
Cuts at: 4009 4009
Size: 8350
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3005 3928 928
Size: 971 1106 923 5350
Fragments arranged by size:
5350 1106 971 923
BbvCI CC'TCA_GC
Cuts at: 4608 4608
Size: 8350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6541 6575 1903
Size: 34 4604 34 3678
Fragments arranged by size:
4604 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2204 6242 7452 2204
Size: 4038 1210 3102
Fragments arranged by size:
4038 3102 1210
BclI T'GATC_A
Cuts at: 1548 2508 5334 1548
Size: 960 2826 4564
Fragments arranged by size:
4564 2826 960
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3629 3833 5117
Size: 107 122 71 631 605 1957 204 1284
Cuts at: 5117 6961 136
Size: 1844 1525
Fragments arranged by size:
1957 1844 1525 1284 631 605 204 122
107 71
BglII A'GATC_T
Cuts at: 5451 8345 5451
Size: 2894 5456
BlpI GC'TnA_GC
Cuts at: 5087 5767 5087
Size: 680 7670
Bme1580I G_kGCm'C
Cuts at: 1790 3085 3296 3403 6411 7567 1790
Size: 1295 211 107 3008 1156 2573
Fragments arranged by size:
3008 2573 1295 1156 211 107
BmgBI CAC'GTC
Cuts at: 1318 2814 3334 3772 1318
Size: 1496 520 438 5896
Fragments arranged by size:
5896 1496 520 438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2719 3673 3815
Size: 416 53 83 399 24 1302 954 142
Cuts at: 3815 5191 6081 7039 442
Size: 1376 890 958 1753
Fragments arranged by size:
1753 1376 1302 958 954 890 416 399
142 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8350
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3913 3913
Size: 8350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 3308 3722 4205 4235 4860 5069 5396
Size: 1361 708 414 483 30 625 209 327
Cuts at: 5396 7010 1239
Size: 1614 2579
Fragments arranged by size:
2579 1614 1361 708 625 483 414 327
209 30
Bpu10I CC'TnA_GC
Cuts at: 3642 4432 4608 3642
Size: 790 176 7384
Fragments arranged by size:
7384 790 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1997 2383 5057 5225 5410 6475 7340 7638 1997
Size: 386 2674 168 185 1065 865 298 2709
Fragments arranged by size:
2709 2674 1065 865 386 298 185 168
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2433 4472 7013 882
Size: 617 934 2039 2541 2219
Fragments arranged by size:
2541 2219 2039 934 617
BsaAI yAC'GTr
Cuts at: 493 1204 3154 6141 493
Size: 711 1950 2987 2702
Fragments arranged by size:
2987 2702 1950 711
BsaBI GATnn'nnATC
Cuts at: 2936 3128 8344 2936
Size: 192 5216 2942
Fragments arranged by size:
5216 2942 192
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 2693 3719 3876
Size: 53 83 186 936 792 368 1026 157
Cuts at: 3876 3958 4006 4734 5151 5181 5358 6157 6539
Size: 82 48 728 417 30 177 799 382
Cuts at: 6539 7174 275
Size: 635 1451
Fragments arranged by size:
1451 1026 936 799 792 728 635 417
382 368 186 177 157 83 82 53
48 30
BsaWI w'CCGG_w
Cuts at: 6783 7181 7455 7602 8013 6783
Size: 398 274 147 411 7120
Fragments arranged by size:
7120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3704 3734 5501 5531 1178
Size: 30 165 30 2301 30 1767 30 3997
Fragments arranged by size:
3997 2301 1767 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 3800 3959 3962 4202 4205 4243 4355
Size: 1633 981 159 3 240 3 38 112
Cuts at: 4355 4772 4850 4901 4940 4943 4952 5125 1186
Size: 417 78 51 39 3 9 173 4411
Fragments arranged by size:
4411 1633 981 417 240 173 159 112
78 51 39 38 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2223 2372 2767 3423 3654 3806 4317 4364 7553
Size: 149 395 656 231 152 511 47 3189
Cuts at: 7553 8049 2223
Size: 496 2524
Fragments arranged by size:
3189 2524 656 511 496 395 231 152
149 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3038 3593 3707 3728 4139 4928 5294
Size: 573 1302 555 114 21 411 789 366
Cuts at: 5294 5608 1163
Size: 314 3905
Fragments arranged by size:
3905 1302 789 573 555 411 366 314
114 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 3974 5126 5800 6561 7589
Size: 275 186 37 1936 1152 674 761 1028
Cuts at: 7589 1540
Size: 2301
Fragments arranged by size:
2301 1936 1152 1028 761 674 275 186
37
BsiHKAI G_wGCw'C
Cuts at: 2746 2865 3403 4061 4107 4692 5088 5402 6411
Size: 119 538 658 46 585 396 314 1009
Cuts at: 6411 6496 7567 2746
Size: 85 1071 3529
Fragments arranged by size:
3529 1071 1009 658 585 538 396 314
119 85 46
BsmI GAATG_Cn'
Cuts at: 4360 5418 5861 5954 4360
Size: 1058 443 93 6756
Fragments arranged by size:
6756 1058 443 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5188 5188
Size: 8350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2746 2790 2865 3085 3296 3403 4061
Size: 399 956 44 75 220 211 107 658
Cuts at: 4061 4107 4408 4692 5088 5135 5402 5669 6411
Size: 46 301 284 396 47 267 267 742
Cuts at: 6411 6496 7567 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 956 742 658 399 396 301
284 267 267 220 211 107 85 75
47 46 44
BspEI T'CCGG_A
Cuts at: 8013 8013
Size: 8350
BspHI T'CATG_A
Cuts at: 2457 6239 7969 2457
Size: 3782 1730 2838
Fragments arranged by size:
3782 2838 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3099 3414 4312 5705 844
Size: 2255 315 898 1393 3489
Fragments arranged by size:
3489 2255 1393 898 315
BsrBI CCG'CTC
Cuts at: 1394 1823 2674 3715 4195 4840 5517 6237 1394
Size: 429 851 1041 480 645 677 720 3507
Fragments arranged by size:
3507 1041 851 720 677 645 480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2988 5259 6845 7019 65
Size: 1183 1740 2271 1586 174 1396
Fragments arranged by size:
2271 1740 1586 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3669 3969 4203 4903 6994 7181 1700
Size: 110 1859 300 234 700 2091 187 2869
Fragments arranged by size:
2869 2091 1859 700 300 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 2514 5508 96
Size: 2418 2994 2938
Fragments arranged by size:
2994 2938 2418
BssHII G'CGCG_C
Cuts at: 1925 4917 1925
Size: 2992 5358
BssSI C'ACGA_G
Cuts at: 3941 5288 6410 7422 3941
Size: 1347 1122 1012 4869
Fragments arranged by size:
4869 1347 1122 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8350
BstBI TT'CG_AA
Cuts at: 7154 7154
Size: 8350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8350
BstXI CCAn_nnnn'nTGG
Cuts at: 2443 5047 2443
Size: 2604 5746
BstYI r'GATC_y
Cuts at: 1972 2349 2991 3435 3465 5451 5680 6047 6432
Size: 377 642 444 30 1986 229 367 385
Cuts at: 6432 6449 7901 7987 7999 8345 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1986 1977 1452 642 444 385 377 367
346 229 86 30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2071 2762 4113 4287 4509 4680
Size: 552 939 67 691 1351 174 222 171
Cuts at: 4680 513
Size: 4183
Fragments arranged by size:
4183 1351 939 691 552 222 174 171
67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2143 3298 4050 4291 487
Size: 683 56 917 1155 752 241 4546
Fragments arranged by size:
4546 1155 917 752 683 241 56
BtsI GCAGTG_nn'
Cuts at: 2865 3050 5596 5866 6660 6680 2865
Size: 185 2546 270 794 20 4535
Fragments arranged by size:
4535 2546 794 270 185 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8315
DraI TTT'AAA
Cuts at: 5654 5654
Size: 8350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2407 2467 4133 5027 7357 809
Size: 507 1091 60 1666 894 2330 1802
Fragments arranged by size:
2330 1802 1666 1091 894 507 60
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2634 3304 3336
Size: 54 1425 50 275 18 804 670 32
Cuts at: 3336 3411 3971 5037 5123 5640 5797 6686 8315
Size: 75 560 1066 86 517 157 889 1629
Cuts at: 8315 8
Size: 43
Fragments arranged by size:
1629 1425 1066 889 804 670 560 517
275 157 86 75 54 50 43 32
18
EagI C'GGCC_G
Cuts at: 1537 1812 3971 5123 5797 1537
Size: 275 2159 1152 674 4090
Fragments arranged by size:
4090 2159 1152 674 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3987 4517 5616 6280 1353
Size: 2634 530 1099 664 3423
Fragments arranged by size:
3423 2634 1099 664 530
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3998 6937 7311 7457 387
Size: 1064 138 2409 2939 374 146 1280
Fragments arranged by size:
2939 2409 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4059 4690 5086 5400 4059
Size: 631 396 314 7009
Fragments arranged by size:
7009 631 396 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2549 2588 2600 3017 3308 3722
Size: 393 533 384 39 12 417 291 414
Cuts at: 3722 3743 4205 4235 4860 5069 5287 5299 5396
Size: 21 462 30 625 209 218 12 97
Cuts at: 5396 6413 7010 7797 8065 1239
Size: 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 787 625 597 533 462 417
414 393 384 291 268 218 209 97
39 30 21 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2488 2488
Size: 8350
EcoO109I rG'GnC_Cy
Cuts at: 2584 2713 3031 3082 4612 5247 2584
Size: 129 318 51 1530 635 5687
Fragments arranged by size:
5687 1530 635 318 129 51
EcoRI G'AATT_C
Cuts at: 5659 6037 5659
Size: 378 7972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 5317 2287
Size: 3030 5320
FspI TGC'GCA
Cuts at: 6856 6856
Size: 8350
HaeII r_GCGC'y
Cuts at: 1678 2901 3642 3798 4009 4270 7497 1678
Size: 1223 741 156 211 261 3227 2531
Fragments arranged by size:
3227 2531 1223 741 261 211 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2234 2329 2361 3704
Size: 32 135 32 630 32 95 32 1343
Cuts at: 3704 3736 3893 3925 4778 4810 5326 5358 6991
Size: 32 157 32 853 32 516 32 1633
Cuts at: 6991 7023 7065 7097 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1633 1343 853 630 516 157 135
95 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5578 5694 5940 669
Size: 988 281 3640 116 246 3079
Fragments arranged by size:
3640 3079 988 281 246 116
HindIII A'AGCT_T
Cuts at: 748 3755 748
Size: 3007 5343
HpaI GTT'AAC
Cuts at: 5940 5940
Size: 8350
KasI G'GCGC_C
Cuts at: 4005 4005
Size: 8350
KpnI G_GTAC'C
Cuts at: 3174 4084 5675 3174
Size: 910 1591 5849
Fragments arranged by size:
5849 1591 910
MfeI C'AATT_G
Cuts at: 5949 5949
Size: 8350
MluI A'CGCG_T
Cuts at: 8226 8226
Size: 8350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3249 3957 5211 7464 7648 1305
Size: 1944 708 1254 2253 184 2007
Fragments arranged by size:
2253 2007 1944 1254 708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 3338 3413 5039 5642
Size: 54 1425 343 804 702 75 1626 603
Cuts at: 5642 10
Size: 2718
Fragments arranged by size:
2718 1626 1425 804 702 603 343 75
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2246 3269 3344 4112 5225 5423
Size: 648 996 84 1023 75 768 1113 198
Cuts at: 5423 6308 6667 6826 518
Size: 885 359 159 2042
Fragments arranged by size:
2042 1113 1023 996 885 768 648 359
198 159 84 75
MspA1I CmG'CkG
Cuts at: 1606 2073 2223 2764 3427 3736 3955 4895 5170
Size: 467 150 541 663 309 219 940 275
Cuts at: 5170 5319 5766 6443 7591 7836 1606
Size: 149 447 677 1148 245 2120
Fragments arranged by size:
2120 1148 940 677 663 541 467 447
309 275 245 219 150 149
NarI GG'CG_CC
Cuts at: 4006 4006
Size: 8350
NcoI C'CATG_G
Cuts at: 513 1065 2004 4509 513
Size: 552 939 2505 4354
Fragments arranged by size:
4354 2505 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8350
NotI GC'GGCC_GC
Cuts at: 5797 5797
Size: 8350
NspI r_CATG'y
Cuts at: 1132 1229 2044 2887 5708 7253 1132
Size: 97 815 843 2821 1545 2229
Fragments arranged by size:
2821 2229 1545 843 815 97
PasI CC'CwG_GG
Cuts at: 1348 3927 1348
Size: 2579 5771
PciI A'CATG_T
Cuts at: 7249 7249
Size: 8350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2709 3328 3511 3661 4324 1108
Size: 1601 619 183 150 663 5134
Fragments arranged by size:
5134 1601 663 619 183 150
PmeI GTTT'AAAC
Cuts at: 5654 5654
Size: 8350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5555 5587 6419 6451 7932 7964 5555
Size: 32 832 32 1481 32 5941
Fragments arranged by size:
5941 1481 832 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2584 2713 3031 4612 5247 2584
Size: 129 318 1581 635 5687
Fragments arranged by size:
5687 1581 635 318 129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8350
PsiI TTA'TAA
Cuts at: 5920 5920
Size: 8350
PspOMI G'GGCC_C
Cuts at: 3081 3081
Size: 8350
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2507 2539 2948 2980 2507
Size: 32 409 32 7877
Fragments arranged by size:
7877 409 32 32
PstI C_TGCA'G
Cuts at: 830 2061 3045 3218 3495 3609 3735 5016 5324
Size: 1231 984 173 277 114 126 1281 308
Cuts at: 5324 5702 830
Size: 378 3478
Fragments arranged by size:
3478 1281 1231 984 378 308 277 173
126 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8350
PvuII CAG'CTG
Cuts at: 2223 3736 5170 5319 2223
Size: 1513 1434 149 5254
Fragments arranged by size:
5254 1513 1434 149
SacI G_AGCT'C
Cuts at: 4061 4692 5088 5402 4061
Size: 631 396 314 7009
Fragments arranged by size:
7009 631 396 314
SacII CC_GC'GG
Cuts at: 2074 2765 2074
Size: 691 7659
SalI G'TCGA_C
Cuts at: 1936 5576 5692 1936
Size: 3640 116 4594
Fragments arranged by size:
4594 3640 116
SanDI GG'GwC_CC
Cuts at: 5247 5247
Size: 8350
SapI GCTCTTCn'nnn_
Cuts at: 3987 4517 3987
Size: 530 7820
SbfI CC_TGCA'GG
Cuts at: 5702 5702
Size: 8350
ScaI AGT'ACT
Cuts at: 6598 8310 6598
Size: 1712 6638
SfcI C'TryA_G
Cuts at: 826 1045 2057 3041 3214 3491 3605 3731 4399
Size: 219 1012 984 173 277 114 126 668
Cuts at: 4399 5012 5320 5350 5698 6833 7514 7705 826
Size: 613 308 30 348 1135 681 191 1471
Fragments arranged by size:
1471 1135 1012 984 681 668 613 348
308 277 219 191 173 126 114 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3833 3833
Size: 8350
SfoI GGC'GCC
Cuts at: 4007 4007
Size: 8350
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8350
SmaI CCC'GGG
Cuts at: 1872 2596 4095 5677 1872
Size: 724 1499 1582 4545
Fragments arranged by size:
4545 1582 1499 724
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 2362 5072 5240 5389 6454
Size: 197 926 69 350 2710 168 149 1065
Cuts at: 6454 7355 7617 820
Size: 901 262 1553
Fragments arranged by size:
2710 1553 1065 926 901 350 262 197
168 149 69
SnaBI TAC'GTA
Cuts at: 493 6141 493
Size: 5648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8350
SphI G_CATG'C
Cuts at: 1132 2887 5708 1132
Size: 1755 2821 3774
Fragments arranged by size:
3774 2821 1755
SspI AAT'ATT
Cuts at: 5 52 6274 5
Size: 47 6222 2081
Fragments arranged by size:
6222 2081 47
StuI AGG'CCT
Cuts at: 1517 3864 1517
Size: 2347 6003
StyI C'CwwG_G
Cuts at: 513 1065 2004 3765 3831 4509 5789 513
Size: 552 939 1761 66 678 1280 3074
Fragments arranged by size:
3074 1761 1280 939 678 552 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3437 3668 6377 6394 6547 6732 7177
Size: 1255 1857 231 2709 17 153 185 445
Cuts at: 7177 325
Size: 1498
Fragments arranged by size:
2709 1857 1498 1255 445 231 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 3234 5508
Size: 274 80 33 51 1443 537 720 2274
Cuts at: 5508 6596 8308 96
Size: 1088 1712 138
Fragments arranged by size:
2274 1712 1443 1088 720 537 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 5456 5488 5575 5607 8310 8342 2996
Size: 32 2428 32 87 32 2703 32 3004
Fragments arranged by size:
3004 2703 2428 87 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2066 4331 5251 1316
Size: 750 2265 920 4415
Fragments arranged by size:
4415 2265 920 750
XbaI T'CTAG_A
Cuts at: 5686 5686
Size: 8350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8350
XmaI C'CCGG_G
Cuts at: 1870 2594 4093 5675 1870
Size: 724 1499 1582 4545
Fragments arranged by size:
4545 1582 1499 724
XmnI GAAnn'nnTTC
Cuts at: 6479 6479
Size: 8350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2326 2694 6158 276
Size: 53 83 186 936 792 368 3464 2468
Fragments arranged by size:
3464 2468 936 792 368 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI RsrII SexAI SrfI SwaI XcmI