Restriction map for
pFN21AB3047
(Circular) (Six-base) MAPSORT of: pFN21AB3047.seq Check: 175 from: 1 to: 8146
pFN21AB3047 8146 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3667 3667
Size: 8146
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5956 278
Size: 53 83 186 936 4420 2468
Fragments arranged by size:
4420 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4904 5046 5489 1937
Size: 2967 142 443 4594
Fragments arranged by size:
4594 2967 443 142
Acc65I G'GTAC_C
Cuts at: 5467 5467
Size: 8146
AclI AA'CG_TT
Cuts at: 1563 2753 3298 6273 6646 1563
Size: 1190 545 2975 373 3063
Fragments arranged by size:
3063 2975 1190 545 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3132 4113 6209 7593 7861 1632
Size: 1500 981 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1500 1384 981 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7949
AflIII A'CryG_T
Cuts at: 1203 4593 4672 7045 8022 1203
Size: 3390 79 2373 977 1327
Fragments arranged by size:
3390 2373 1327 977 79
AgeI A'CCGG_T
Cuts at: 6977 6977
Size: 8146
AhdI GACnn_n'nnGTC
Cuts at: 6875 6875
Size: 8146
AleI CACnn'nnGTG
Cuts at: 1166 3251 1166
Size: 2085 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8146
AlwNI CAG_nnn'CTG
Cuts at: 4936 5130 5889 7461 4936
Size: 194 759 1572 5621
Fragments arranged by size:
5621 1572 759 194
ApaI G_GGCC'C
Cuts at: 4544 4544
Size: 8146
ApaLI G'TGCA_C
Cuts at: 4467 6203 7359 4467
Size: 1736 1156 5254
Fragments arranged by size:
5254 1736 1156
ApoI r'AATT_y
Cuts at: 1493 2018 2295 3715 3751 3902 4200 4236 5455
Size: 525 277 1420 36 151 298 36 1219
Cuts at: 5455 5681 5833 7942 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1420 1219 525 298 277 226
152 151 36 36
AseI AT'TA_AT
Cuts at: 160 1032 4211 6700 160
Size: 872 3179 2489 1606
Fragments arranged by size:
3179 2489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8146
AvaI C'yCGr_G
Cuts at: 1870 1943 4326 5471 1870
Size: 73 2383 1145 4545
Fragments arranged by size:
4545 2383 1145 73
AvrII C'CTAG_G
Cuts at: 2855 2855
Size: 8146
BamHI G'GATC_C
Cuts at: 2308 5164 5476 5843 2308
Size: 2856 312 367 4611
Fragments arranged by size:
4611 2856 367 312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5467 6922 618
Size: 325 217 627 3680 1455 1842
Fragments arranged by size:
3680 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2990 3191 4544 5415 5465 1391
Size: 1599 201 1353 871 50 4072
Fragments arranged by size:
4072 1599 1353 871 201 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4790 5106 928
Size: 971 2891 316 3968
Fragments arranged by size:
3968 2891 971 316
BbvCI CC'TCA_GC
Cuts at: 3684 3684
Size: 8146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6337 6371 1903
Size: 34 4400 34 3678
Fragments arranged by size:
4400 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 6038 7248 4001
Size: 2037 1210 4899
Fragments arranged by size:
4899 2037 1210
BclI T'GATC_A
Cuts at: 1548 5358 1548
Size: 3810 4336
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6757 136
Size: 107 122 71 631 605 5085 1525
Fragments arranged by size:
5085 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2393 2531 4615 8141 2393
Size: 138 2084 3526 2398
Fragments arranged by size:
3526 2398 2084 138
BlpI GC'TnA_GC
Cuts at: 5563 5563
Size: 8146
Bme1580I G_kGCm'C
Cuts at: 1790 4471 4544 6207 7363 1790
Size: 2681 73 1663 1156 2573
Fragments arranged by size:
2681 2573 1663 1156 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8146
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4858 5877 6835
Size: 416 53 83 399 24 3441 1019 958
Cuts at: 6835 442
Size: 1753
Fragments arranged by size:
3441 1753 1019 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 3069 3278 3533 4377 4596 4813 4857
Size: 1430 400 209 255 844 219 217 44
Cuts at: 4857 4951 6806 1239
Size: 94 1855 2579
Fragments arranged by size:
2579 1855 1430 844 400 255 219 217
209 94 44
Bpu10I CC'TnA_GC
Cuts at: 3684 3684
Size: 8146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3773 6271 7136 7434 3773
Size: 2498 865 298 4485
Fragments arranged by size:
4485 2498 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6809 882
Size: 617 5310 2219
Fragments arranged by size:
5310 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4018 4675 5937 493
Size: 711 2814 657 1262 2702
Fragments arranged by size:
2814 2702 1262 711 657
BsaBI GATnn'nnATC
Cuts at: 4266 8140 4266
Size: 3874 4272
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5953 6335 6970 275
Size: 53 83 186 936 4420 382 635 1451
Fragments arranged by size:
4420 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4776 6579 6977 7251 7398 7809 4776
Size: 1803 398 274 147 411 5113
Fragments arranged by size:
5113 1803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2642 2672 3046 3076 3831
Size: 30 165 30 1239 30 374 30 755
Cuts at: 3831 3861 1178
Size: 30 5463
Fragments arranged by size:
5463 1239 755 374 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 3701 4595 1186
Size: 1054 1461 894 4737
Fragments arranged by size:
4737 1461 1054 894
BseYI C'CCAG_C
Cuts at: 4888 5130 7349 7845 4888
Size: 242 2219 496 5189
Fragments arranged by size:
5189 2219 496 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5596 6357 7385 1540
Size: 275 186 3595 761 1028 2301
Fragments arranged by size:
3595 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2990 4471 6207 6292 7363 2990
Size: 1481 1736 85 1071 3773
Fragments arranged by size:
3773 1736 1481 1071 85
BsmI GAATG_Cn'
Cuts at: 4033 5657 5750 4033
Size: 1624 93 6429
Fragments arranged by size:
6429 1624 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2222 2222
Size: 8146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2990 3191 4471 4544 5415 5465 6207
Size: 399 1200 201 1280 73 871 50 742
Cuts at: 6207 6292 7363 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1280 1200 1071 871 742 399 201
85 73 50
BspEI T'CCGG_A
Cuts at: 4776 7809 4776
Size: 3033 5113
BspHI T'CATG_A
Cuts at: 4627 6035 7765 4627
Size: 1408 1730 5008
Fragments arranged by size:
5008 1730 1408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2227 3667 5501 844
Size: 1383 1440 1834 3489
Fragments arranged by size:
3489 1834 1440 1383
BsrBI CCG'CTC
Cuts at: 1394 1823 6033 1394
Size: 429 4210 3507
Fragments arranged by size:
4210 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4189 4264 6641 6815 65
Size: 1183 2941 75 2377 174 1396
Fragments arranged by size:
2941 2377 1396 1183 174 75
BsrFI r'CCGG_y
Cuts at: 1700 1810 6790 6977 1700
Size: 110 4980 187 2869
Fragments arranged by size:
4980 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5020 96
Size: 4924 3222
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8146
BssSI C'ACGA_G
Cuts at: 6206 7218 6206
Size: 1012 7134
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4717 794
Size: 3923 4223
BstBI TT'CG_AA
Cuts at: 5438 6950 5438
Size: 1512 6634
BstEII G'GTnAC_C
Cuts at: 1184 3812 4754 1184
Size: 2628 942 4576
Fragments arranged by size:
4576 2628 942
BstXI CCAn_nnnn'nTGG
Cuts at: 3184 3184
Size: 8146
BstYI r'GATC_y
Cuts at: 1972 2308 2393 2531 4615 4773 5164 5476 5843
Size: 336 85 138 2084 158 391 312 367
Cuts at: 5843 6228 6245 7697 7783 7795 8141 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2084 1977 1452 391 385 367 346 336
312 158 138 86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6655
Fragments arranged by size:
6655 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2596 4764 487
Size: 683 56 1370 2168 3869
Fragments arranged by size:
3869 2168 1370 683 56
BtsI GCAGTG_nn'
Cuts at: 3739 5662 6456 6476 3739
Size: 1923 794 20 5409
Fragments arranged by size:
5409 1923 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8146
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8111
DraI TTT'AAA
Cuts at: 2106 3934 4369 4380 5450 2106
Size: 1828 435 11 1070 4802
Fragments arranged by size:
4802 1828 1070 435 11
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7153 809
Size: 507 5837 1802
Fragments arranged by size:
5837 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5593 6482 8111
Size: 54 1425 50 275 18 3763 889 1629
Cuts at: 8111 8
Size: 43
Fragments arranged by size:
3763 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5593 1537
Size: 275 3781 4090
Fragments arranged by size:
4090 3781 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 2941 2976 4132 4655 6076 1353
Size: 1314 274 35 1156 523 1421 3423
Fragments arranged by size:
3423 1421 1314 1156 523 274 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6733 7107 7253 387
Size: 1064 138 5144 374 146 1280
Fragments arranged by size:
5144 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2988 2988
Size: 8146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 3069 3132 3278 3533 4113 4377
Size: 393 1037 400 63 146 255 580 264
Cuts at: 4377 4596 4813 4857 4951 6209 6806 7593 7861
Size: 219 217 44 94 1258 597 787 268
Cuts at: 7861 1239
Size: 1524
Fragments arranged by size:
1524 1258 1037 787 597 580 400 393
268 264 255 219 217 146 94 63
44
EcoO109I rG'GnC_Cy
Cuts at: 3173 3953 4933 3173
Size: 780 980 6386
Fragments arranged by size:
6386 980 780
EcoRI G'AATT_C
Cuts at: 4200 5455 5833 4200
Size: 1255 378 6513
Fragments arranged by size:
6513 1255 378
EcoRV GAT'ATC
Cuts at: 4432 4531 4432
Size: 99 8047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5231 5231
Size: 8146
FspI TGC'GCA
Cuts at: 4809 6652 4809
Size: 1843 6303
HaeII r_GCGC'y
Cuts at: 1678 7293 1678
Size: 5615 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2836 2868 3046
Size: 32 135 32 632 32 600 32 178
Cuts at: 3046 3078 3269 3301 3908 3940 4270 4302 4375
Size: 32 191 32 607 32 330 32 73
Cuts at: 4375 4407 4982 5014 5300 5332 6787 6819 6861
Size: 32 575 32 286 32 1455 32 42
Cuts at: 6861 6893 1373
Size: 32 2626
Fragments arranged by size:
2626 1455 632 607 600 575 330 286
191 178 135 73 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5490 5736 669
Size: 988 281 3552 246 3079
Fragments arranged by size:
3552 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3144 748
Size: 2396 5750
HpaI GTT'AAC
Cuts at: 5736 5736
Size: 8146
KpnI G_GTAC'C
Cuts at: 5471 5471
Size: 8146
MfeI C'AATT_G
Cuts at: 5745 5745
Size: 8146
MluI A'CGCG_T
Cuts at: 8022 8022
Size: 8146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2679 7260 7444 1305
Size: 1374 4581 184 2007
Fragments arranged by size:
4581 2007 1374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6324
Fragments arranged by size:
6324 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 3251 4673 6104 6463 6622 518
Size: 648 1501 584 1422 1431 359 159 2042
Fragments arranged by size:
2042 1501 1431 1422 648 584 359 159
MspA1I CmG'CkG
Cuts at: 1606 2915 4574 5130 5562 6239 7387 7632 1606
Size: 1309 1659 556 432 677 1148 245 2120
Fragments arranged by size:
2120 1659 1309 1148 677 556 432 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6655
Fragments arranged by size:
6655 939 552
NdeI CA'TA_TG
Cuts at: 387 4745 4947 387
Size: 4358 202 3586
Fragments arranged by size:
4358 3586 202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8146
NotI GC'GGCC_GC
Cuts at: 5593 5593
Size: 8146
NspI r_CATG'y
Cuts at: 1132 1229 4148 4597 5504 7049 1132
Size: 97 2919 449 907 1545 2229
Fragments arranged by size:
2919 2229 1545 907 449 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8146
PciI A'CATG_T
Cuts at: 4593 7045 4593
Size: 2452 5694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8146
PfoI T'CCnGG_A
Cuts at: 4656 4834 4656
Size: 178 7968
PmeI GTTT'AAAC
Cuts at: 5450 5450
Size: 8146
PmlI CAC'GTG
Cuts at: 4675 4675
Size: 8146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6215 6247 7728 7760 6215
Size: 32 1481 32 6601
Fragments arranged by size:
6601 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3173 4933 3173
Size: 1760 6386
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8146
PsiI TTA'TAA
Cuts at: 2994 5716 2994
Size: 2722 5424
PspOMI G'GGCC_C
Cuts at: 4540 4540
Size: 8146
PstI C_TGCA'G
Cuts at: 830 5498 830
Size: 4668 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8146
PvuII CAG'CTG
Cuts at: 2915 4574 5130 2915
Size: 1659 556 5931
Fragments arranged by size:
5931 1659 556
SacI G_AGCT'C
Cuts at: 2990 2990
Size: 8146
SalI G'TCGA_C
Cuts at: 1936 5488 1936
Size: 3552 4594
SbfI CC_TGCA'GG
Cuts at: 5498 5498
Size: 8146
ScaI AGT'ACT
Cuts at: 2499 6394 8106 2499
Size: 3895 1712 2539
Fragments arranged by size:
3895 2539 1712
SexAI A'CCwGG_T
Cuts at: 2287 2287
Size: 8146
SfcI C'TryA_G
Cuts at: 826 1045 2848 4683 4905 5340 5494 6629 7310
Size: 219 1803 1835 222 435 154 1135 681
Cuts at: 7310 7501 826
Size: 191 1471
Fragments arranged by size:
1835 1803 1471 1135 681 435 222 219
191 154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8146
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8146
SmaI CCC'GGG
Cuts at: 1872 5473 1872
Size: 3601 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3788 6250 7151 7413 820
Size: 197 926 1845 2462 901 262 1553
Fragments arranged by size:
2462 1845 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5937 493
Size: 5444 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8146
SphI G_CATG'C
Cuts at: 1132 5504 1132
Size: 4372 3774
SspI AAT'ATT
Cuts at: 5 52 6070 5
Size: 47 6018 2081
Fragments arranged by size:
6018 2081 47
StuI AGG'CCT
Cuts at: 1517 3231 1517
Size: 1714 6432
StyI C'CwwG_G
Cuts at: 513 1065 2004 2855 2943 3177 3396 3538 5169
Size: 552 939 851 88 234 219 142 1631
Cuts at: 5169 5585 513
Size: 416 3074
Fragments arranged by size:
3074 1631 939 851 552 416 234 219
142 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2503 3751 6173 6190 6343 6528 6973
Size: 1255 923 1248 2422 17 153 185 445
Cuts at: 6973 325
Size: 1498
Fragments arranged by size:
2422 1498 1255 1248 923 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2497 5020 5053
Size: 274 80 33 51 1443 520 2523 33
Cuts at: 5053 6392 8104 96
Size: 1339 1712 138
Fragments arranged by size:
2523 1712 1443 1339 520 274 138 80
51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8106 8138 8106
Size: 32 8114
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8146
XbaI T'CTAG_A
Cuts at: 5249 5482 5249
Size: 233 7913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2956 3985 2956
Size: 1029 7117
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8146
XmaI C'CCGG_G
Cuts at: 1870 5471 1870
Size: 3601 4545
XmnI GAAnn'nnTTC
Cuts at: 5434 6275 5434
Size: 841 7305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5954 276
Size: 53 83 186 936 4420 2468
Fragments arranged by size:
4420 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI