Restriction map for
pFN21AB3046
(Circular) (Six-base) MAPSORT of: pFN21AB3046.seq Check: 8700 from: 1 to: 9226
pFN21AB3046 9226 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5612 5612
Size: 9226
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7036 278
Size: 53 83 186 936 5500 2468
Fragments arranged by size:
5500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2694 3471 6569 1937
Size: 757 777 3098 4594
Fragments arranged by size:
4594 3098 777 757
Acc65I G'GTAC_C
Cuts at: 2262 2555 6547 2262
Size: 293 3992 4941
Fragments arranged by size:
4941 3992 293
AclI AA'CG_TT
Cuts at: 1563 7353 7726 1563
Size: 5790 373 3063
Fragments arranged by size:
5790 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2766 3618 3767 5342 6468 7289 8673
Size: 455 679 852 149 1575 1126 821 1384
Cuts at: 8673 8941 1632
Size: 268 1917
Fragments arranged by size:
1917 1575 1384 1126 852 821 679 455
268 149
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9029
AflIII A'CryG_T
Cuts at: 1203 4571 4608 5608 8125 9102 1203
Size: 3368 37 1000 2517 977 1327
Fragments arranged by size:
3368 2517 1327 1000 977 37
AgeI A'CCGG_T
Cuts at: 8057 8057
Size: 9226
AhdI GACnn_n'nnGTC
Cuts at: 4609 7955 4609
Size: 3346 5880
AleI CACnn'nnGTG
Cuts at: 1166 2681 4005 5867 6245 1166
Size: 1515 1324 1862 378 4147
Fragments arranged by size:
4147 1862 1515 1324 378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6525 1184
Size: 5341 3885
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4271 4303 4742 4774 4271
Size: 32 439 32 8723
Fragments arranged by size:
8723 439 32 32
AlwNI CAG_nnn'CTG
Cuts at: 6969 8541 6969
Size: 1572 7654
ApaI G_GGCC'C
Cuts at: 5545 5545
Size: 9226
ApaLI G'TGCA_C
Cuts at: 2750 7283 8439 2750
Size: 4533 1156 3537
Fragments arranged by size:
4533 3537 1156
ApoI r'AATT_y
Cuts at: 1493 2114 2576 2620 3344 3882 4997 5375 5474
Size: 621 462 44 724 538 1115 378 99
Cuts at: 5474 5528 6356 6535 6761 6913 9022 1493
Size: 54 828 179 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 828 724 621 538 462
378 226 179 152 99 54 44
AseI AT'TA_AT
Cuts at: 160 1032 7780 160
Size: 872 6748 1606
Fragments arranged by size:
6748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
AvaI C'yCGr_G
Cuts at: 1870 1943 3169 3279 5544 5632 6551 1870
Size: 73 1226 110 2265 88 919 4545
Fragments arranged by size:
4545 2265 1226 919 110 88 73
AvrII C'CTAG_G
Cuts at: 4252 4252
Size: 9226
BamHI G'GATC_C
Cuts at: 2605 6556 6923 2605
Size: 3951 367 4908
Fragments arranged by size:
4908 3951 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 2555 2939 5335 6547
Size: 325 217 627 475 293 384 2396 1212
Cuts at: 6547 8002 618
Size: 1455 1842
Fragments arranged by size:
2396 1842 1455 1212 627 475 384 325
293 217
BanII G_rGCy'C
Cuts at: 1391 2344 4707 4794 5545 6545 1391
Size: 953 2363 87 751 1000 4072
Fragments arranged by size:
4072 2363 1000 953 751 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 3666 4301 5057 928
Size: 971 176 1591 635 756 5097
Fragments arranged by size:
5097 1591 971 756 635 176
BbvCI CC'TCA_GC
Cuts at: 2631 3851 4817 5283 2631
Size: 1220 966 466 6574
Fragments arranged by size:
6574 1220 966 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3711 3745 6446 6480 7417
Size: 34 50 34 1690 34 2701 34 937
Cuts at: 7417 7451 1903
Size: 34 3678
Fragments arranged by size:
3678 2701 1690 937 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3457 4297 5033 7118 8328 3457
Size: 840 736 2085 1210 4355
Fragments arranged by size:
4355 2085 1210 840 736
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9226
BfrBI ATG'CAT
Cuts at: 3121 3121
Size: 9226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4098 7837 136
Size: 107 122 71 631 605 2426 3739 1525
Fragments arranged by size:
3739 2426 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4537 9221 4537
Size: 4684 4542
BlpI GC'TnA_GC
Cuts at: 6643 6643
Size: 9226
Bme1580I G_kGCm'C
Cuts at: 1790 2754 5258 5545 7287 8443 1790
Size: 964 2504 287 1742 1156 2573
Fragments arranged by size:
2573 2504 1742 1156 964 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2378 3767 3809
Size: 416 53 83 399 24 961 1389 42
Cuts at: 3809 5507 6239 6957 7915 442
Size: 1698 732 718 958 1753
Fragments arranged by size:
1753 1698 1389 961 958 732 718 416
399 83 53 42 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 5308 5823 3617
Size: 1691 515 7020
Fragments arranged by size:
7020 1691 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3360 6042 7886 1239
Size: 2121 2682 1844 2579
Fragments arranged by size:
2682 2579 2121 1844
Bpu10I CC'TnA_GC
Cuts at: 2631 3814 3851 4817 5283 5331 2631
Size: 1183 37 966 466 48 6526
Fragments arranged by size:
6526 1183 966 466 48 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3348 3587 5818 6246 7351 8216 8514 3348
Size: 239 2231 428 1105 865 298 4060
Fragments arranged by size:
4060 2231 1105 865 428 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7889 882
Size: 617 6390 2219
Fragments arranged by size:
6390 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7017 493
Size: 711 5813 2702
Fragments arranged by size:
5813 2702 711
BsaBI GATnn'nnATC
Cuts at: 2804 6119 9220 2804
Size: 3315 3101 2810
Fragments arranged by size:
3315 3101 2810
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7033 7415 8050 275
Size: 53 83 186 936 5500 382 635 1451
Fragments arranged by size:
5500 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7659 8057 8331 8478 8889 7659
Size: 398 274 147 411 7996
Fragments arranged by size:
7996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2733 2763 2879 2909 4742
Size: 30 165 30 1330 30 116 30 1833
Cuts at: 4742 4772 4805 4835 5871 5901 1178
Size: 30 33 30 1036 30 4503
Fragments arranged by size:
4503 1833 1330 1036 165 116 33 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2754 4457 6404 1186
Size: 1568 1703 1947 4008
Fragments arranged by size:
4008 1947 1703 1568
BseYI C'CCAG_C
Cuts at: 2827 4350 6278 8429 8925 2827
Size: 1523 1928 2151 496 3128
Fragments arranged by size:
3128 2151 1928 1523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 3167 6476 1163
Size: 573 346 1085 3309 3913
Fragments arranged by size:
3913 3309 1085 573 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6676 7437 8465 1540
Size: 275 186 4675 761 1028 2301
Fragments arranged by size:
4675 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2344 2754 3603 5090 5511 7287 7372 8443 2344
Size: 410 849 1487 421 1776 85 1071 3127
Fragments arranged by size:
3127 1776 1487 1071 849 421 410 85
BsmI GAATG_Cn'
Cuts at: 2532 4118 4177 4229 6737 6830 2532
Size: 1586 59 52 2508 93 4928
Fragments arranged by size:
4928 2508 1586 93 59 52
BsmBI CGTCTCn'nnnn_
Cuts at: 4060 4508 6264 4060
Size: 448 1756 7022
Fragments arranged by size:
7022 1756 448
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2344 2754 3603 4707 4794 5090 5258
Size: 399 554 410 849 1104 87 296 168
Cuts at: 5258 5511 5545 6545 7287 7372 8443 1391
Size: 253 34 1000 742 85 1071 2174
Fragments arranged by size:
2174 1104 1071 1000 849 742 554 410
399 296 253 168 87 85 34
BspEI T'CCGG_A
Cuts at: 8889 8889
Size: 9226
BspHI T'CATG_A
Cuts at: 7115 8845 7115
Size: 1730 7496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5612 6581 844
Size: 4768 969 3489
Fragments arranged by size:
4768 3489 969
BsrBI CCG'CTC
Cuts at: 1394 1823 7113 1394
Size: 429 5290 3507
Fragments arranged by size:
5290 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2483 3420 4233 4584 6078 7721 7895
Size: 1183 1235 937 813 351 1494 1643 174
Cuts at: 7895 65
Size: 1396
Fragments arranged by size:
1643 1494 1396 1235 1183 937 813 351
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7870 8057 1700
Size: 110 6060 187 2869
Fragments arranged by size:
6060 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9226
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9226
BssSI C'ACGA_G
Cuts at: 7286 8298 7286
Size: 1012 8214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3272 4235 4247 794
Size: 2478 963 12 5773
Fragments arranged by size:
5773 2478 963 12
BstBI TT'CG_AA
Cuts at: 8030 8030
Size: 9226
BstEII G'GTnAC_C
Cuts at: 1184 3163 4333 6430 1184
Size: 1979 1170 2097 3980
Fragments arranged by size:
3980 2097 1979 1170
BstXI CCAn_nnnn'nTGG
Cuts at: 2683 3445 6247 6252 2683
Size: 762 2802 5 5657
Fragments arranged by size:
5657 2802 762 5
BstYI r'GATC_y
Cuts at: 1972 2605 3359 4354 4537 6556 6923 7308 7325
Size: 633 754 995 183 2019 367 385 17
Cuts at: 7325 8777 8863 8875 9221 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2019 1977 1452 995 754 633 385 367
346 183 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2961 3990 4945 6195 513
Size: 552 1896 1029 955 1250 3544
Fragments arranged by size:
3544 1896 1250 1029 955 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8487
Fragments arranged by size:
8487 683 56
BtsI GCAGTG_nn'
Cuts at: 3729 4996 5030 6742 7536 7556 3729
Size: 1267 34 1712 794 20 5399
Fragments arranged by size:
5399 1712 1267 794 34 20
ClaI AT'CG_AT
Cuts at: 1554 3731 1554
Size: 2177 7049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5370 5405 529
Size: 35 4806 35 4350
Fragments arranged by size:
4806 4350 35 35
DraI TTT'AAA
Cuts at: 4680 6530 4680
Size: 1850 7376
DraIII CAC_nnn'GTG
Cuts at: 3803 5515 3803
Size: 1712 7514
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3956 8233 809
Size: 507 2640 4277 1802
Fragments arranged by size:
4277 2640 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 2313 2964
Size: 54 1425 50 275 18 379 104 651
Cuts at: 2964 3553 4523 5859 6137 6254 6673 7562 9191
Size: 589 970 1336 278 117 419 889 1629
Cuts at: 9191 8
Size: 43
Fragments arranged by size:
1629 1425 1336 970 889 651 589 419
379 278 275 117 104 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6673 1537
Size: 275 4861 4090
Fragments arranged by size:
4861 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5118 5478 6292 7156 1353
Size: 3765 360 814 864 3423
Fragments arranged by size:
3765 3423 864 814 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2711 3778 7813 8187 8333 387
Size: 1064 138 1122 1067 4035 374 146 1280
Fragments arranged by size:
4035 1280 1122 1067 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2342 2342
Size: 9226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2766 3360 3618 3767 5342 6042
Size: 393 455 679 594 258 149 1575 700
Cuts at: 6042 6468 7289 7886 8673 8941 1239
Size: 426 821 597 787 268 1524
Fragments arranged by size:
1575 1524 821 787 700 679 597 594
455 426 393 268 258 149
EcoNI CCTnn'n_nnAGG
Cuts at: 5483 5483
Size: 9226
EcoO109I rG'GnC_Cy
Cuts at: 5541 5541
Size: 9226
EcoRI G'AATT_C
Cuts at: 6535 6913 6535
Size: 378 8848
FspI TGC'GCA
Cuts at: 7732 7732
Size: 9226
HaeII r_GCGC'y
Cuts at: 1678 8373 1678
Size: 6695 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 2289 2321 4595
Size: 32 135 32 543 32 142 32 2274
Cuts at: 4595 4627 7867 7899 7941 7973 1373
Size: 32 3240 32 42 32 2626
Fragments arranged by size:
3240 2626 2274 543 142 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3094 5039 5845 6570 6816 669
Size: 988 281 1156 1945 806 725 246 3079
Fragments arranged by size:
3079 1945 1156 988 806 725 281 246
HindIII A'AGCT_T
Cuts at: 748 4458 748
Size: 3710 5516
HpaI GTT'AAC
Cuts at: 6816 6816
Size: 9226
KpnI G_GTAC'C
Cuts at: 2266 2559 6551 2266
Size: 293 3992 4941
Fragments arranged by size:
4941 3992 293
MfeI C'AATT_G
Cuts at: 3952 6825 3952
Size: 2873 6353
MluI A'CGCG_T
Cuts at: 4571 9102 4571
Size: 4531 4695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3890 5173 5930 8340 8524 1305
Size: 2585 1283 757 2410 184 2007
Fragments arranged by size:
2585 2410 2007 1283 757 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2211 2315 2966 3555 4525
Size: 54 1425 343 379 104 651 589 970
Cuts at: 4525 5861 10
Size: 1336 3375
Fragments arranged by size:
3375 1425 1336 970 651 589 379 343
104 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 2681 2783 3039 4005 4166 5648
Size: 648 1359 156 102 256 966 161 1482
Cuts at: 5648 5855 5867 6245 7184 7543 7702 518
Size: 207 12 378 939 359 159 2042
Fragments arranged by size:
2042 1482 1359 966 939 648 378 359
256 207 161 159 156 102 12
MspA1I CmG'CkG
Cuts at: 1606 3670 4350 5306 6392 6642 7319 8467 8712
Size: 2064 680 956 1086 250 677 1148 245
Cuts at: 8712 1606
Size: 2120
Fragments arranged by size:
2120 2064 1148 1086 956 680 677 250
245
NcoI C'CATG_G
Cuts at: 513 1065 2961 3990 4945 513
Size: 552 1896 1029 955 4794
Fragments arranged by size:
4794 1896 1029 955 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9226
NotI GC'GGCC_GC
Cuts at: 6673 6673
Size: 9226
NsiI A_TGCA'T
Cuts at: 3123 3123
Size: 9226
NspI r_CATG'y
Cuts at: 1132 1229 4612 5172 5612 6584 8129 1132
Size: 97 3383 560 440 972 1545 2229
Fragments arranged by size:
3383 2229 1545 972 560 440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9226
PciI A'CATG_T
Cuts at: 4608 5608 8125 4608
Size: 1000 2517 5709
Fragments arranged by size:
5709 2517 1000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2455 2897 3886 1108
Size: 1347 442 989 6448
Fragments arranged by size:
6448 1347 989 442
PfoI T'CCnGG_A
Cuts at: 2172 4289 2172
Size: 2117 7109
PmeI GTTT'AAAC
Cuts at: 6530 6530
Size: 9226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4742 4774 7295 7327 8808 8840 4742
Size: 32 2521 32 1481 32 5128
Fragments arranged by size:
5128 2521 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9226
PsiI TTA'TAA
Cuts at: 6796 6796
Size: 9226
PspOMI G'GGCC_C
Cuts at: 5541 5541
Size: 9226
PstI C_TGCA'G
Cuts at: 830 4873 5271 6578 830
Size: 4043 398 1307 3478
Fragments arranged by size:
4043 3478 1307 398
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9226
PvuII CAG'CTG
Cuts at: 3670 5306 6392 3670
Size: 1636 1086 6504
Fragments arranged by size:
6504 1636 1086
SacI G_AGCT'C
Cuts at: 2344 2344
Size: 9226
SalI G'TCGA_C
Cuts at: 1936 6568 1936
Size: 4632 4594
SbfI CC_TGCA'GG
Cuts at: 6578 6578
Size: 9226
ScaI AGT'ACT
Cuts at: 7474 9186 7474
Size: 1712 7514
SfcI C'TryA_G
Cuts at: 826 1045 2483 2691 4482 4869 5267 6496 6574
Size: 219 1438 208 1791 387 398 1229 78
Cuts at: 6574 7709 8390 8581 826
Size: 1135 681 191 1471
Fragments arranged by size:
1791 1471 1438 1229 1135 681 398 387
219 208 191 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9226
SmaI CCC'GGG
Cuts at: 1872 3171 6553 1872
Size: 1299 3382 4545
Fragments arranged by size:
4545 3382 1299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3363 3602 5797 6261 7330 8231
Size: 197 926 1420 239 2195 464 1069 901
Cuts at: 8231 8493 820
Size: 262 1553
Fragments arranged by size:
2195 1553 1420 1069 926 901 464 262
239 197
SnaBI TAC'GTA
Cuts at: 493 7017 493
Size: 6524 2702
SpeI A'CTAG_T
Cuts at: 152 3268 152
Size: 3116 6110
SphI G_CATG'C
Cuts at: 1132 6584 1132
Size: 5452 3774
SspI AAT'ATT
Cuts at: 5 52 2384 7150 5
Size: 47 2332 4766 2081
Fragments arranged by size:
4766 2332 2081 47
StuI AGG'CCT
Cuts at: 1517 3863 5413 1517
Size: 2346 1550 5330
Fragments arranged by size:
5330 2346 1550
StyI C'CwwG_G
Cuts at: 513 1065 2643 2961 3045 3990 4252 4590 4786
Size: 552 1578 318 84 945 262 338 196
Cuts at: 4786 4841 4945 5133 5339 5674 5865 6148 6665
Size: 55 104 188 206 335 191 283 517
Cuts at: 6665 513
Size: 3074
Fragments arranged by size:
3074 1578 945 552 517 338 335 318
283 262 206 196 191 188 104 84
55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3171 3539 3791 3983 4097 4491 5526
Size: 1255 1591 368 252 192 114 394 1035
Cuts at: 5526 7253 7270 7423 7608 8053 325
Size: 1727 17 153 185 445 1498
Fragments arranged by size:
1727 1591 1498 1255 1035 445 394 368
252 192 185 153 114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2906 5115 6504
Size: 274 80 33 51 1443 929 2209 1389
Cuts at: 6504 7472 9184 96
Size: 968 1712 138
Fragments arranged by size:
2209 1712 1443 1389 968 929 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 2733 2765 4327 4359 4941 4973 9186
Size: 32 547 32 1562 32 582 32 4213
Cuts at: 9186 9218 2154
Size: 32 2162
Fragments arranged by size:
4213 2162 1562 582 547 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9226
XbaI T'CTAG_A
Cuts at: 6562 6562
Size: 9226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3883 6025 3883
Size: 2142 7084
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9226
XmaI C'CCGG_G
Cuts at: 1870 3169 6551 1870
Size: 1299 3382 4545
Fragments arranged by size:
4545 3382 1299
XmnI GAAnn'nnTTC
Cuts at: 2496 3933 5474 6048 7355 2496
Size: 1437 1541 574 1307 4367
Fragments arranged by size:
4367 1541 1437 1307 574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7034 276
Size: 53 83 186 936 5500 2468
Fragments arranged by size:
5500 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BsiWI BstZ17I Bsu36I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI