Restriction map for
pFN21AB3043
(Circular) (Six-base) MAPSORT of: pFN21AB3043.seq Check: 4362 from: 1 to: 8161
pFN21AB3043 8161 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5971 278
Size: 53 83 186 936 4435 2468
Fragments arranged by size:
4435 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3801 4659 4779 5504 1937
Size: 1864 858 120 725 4594
Fragments arranged by size:
4594 1864 858 725 120
Acc65I G'GTAC_C
Cuts at: 5482 5482
Size: 8161
AclI AA'CG_TT
Cuts at: 1563 6288 6661 1563
Size: 4725 373 3063
Fragments arranged by size:
4725 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2604 2987 3052 4339 6224 7608 7876
Size: 479 493 383 65 1287 1885 1384 268
Cuts at: 7876 1632
Size: 1917
Fragments arranged by size:
1917 1885 1384 1287 493 479 383 268
65
AfeI AGC'GCT
Cuts at: 3073 4517 5012 3073
Size: 1444 495 6222
Fragments arranged by size:
6222 1444 495
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7964
AflIII A'CryG_T
Cuts at: 1203 2262 2352 2937 7060 8037 1203
Size: 1059 90 585 4123 977 1327
Fragments arranged by size:
4123 1327 1059 977 585 90
AgeI A'CCGG_T
Cuts at: 6992 6992
Size: 8161
AhdI GACnn_n'nnGTC
Cuts at: 2043 6890 2043
Size: 4847 3314
AleI CACnn'nnGTG
Cuts at: 1166 2357 4565 1166
Size: 1191 2208 4762
Fragments arranged by size:
4762 2208 1191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3537 1184
Size: 2353 5808
AlwNI CAG_nnn'CTG
Cuts at: 2311 3686 3763 3963 4418 5904 7476 2311
Size: 1375 77 200 455 1486 1572 2996
Fragments arranged by size:
2996 1572 1486 1375 455 200 77
ApaI G_GGCC'C
Cuts at: 3442 3631 4395 3442
Size: 189 764 7208
Fragments arranged by size:
7208 764 189
ApaLI G'TGCA_C
Cuts at: 2330 6218 7374 2330
Size: 3888 1156 3117
Fragments arranged by size:
3888 3117 1156
ApoI r'AATT_y
Cuts at: 1493 2588 4745 5129 5470 5696 5848 7957 1493
Size: 1095 2157 384 341 226 152 2109 1697
Fragments arranged by size:
2157 2109 1697 1095 384 341 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6715 160
Size: 872 5683 1606
Fragments arranged by size:
5683 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8161
AvaI C'yCGr_G
Cuts at: 1870 1943 3462 4395 4987 5443 5486 1870
Size: 73 1519 933 592 456 43 4545
Fragments arranged by size:
4545 1519 933 592 456 73 43
BamHI G'GATC_C
Cuts at: 2132 2384 5491 5858 2132
Size: 252 3107 367 4435
Fragments arranged by size:
4435 3107 367 252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3271 3353 3365 4533 4921
Size: 325 217 627 1484 82 12 1168 388
Cuts at: 4921 5482 6937 618
Size: 561 1455 1842
Fragments arranged by size:
1842 1484 1455 1168 627 561 388 325
217 82 12
BanII G_rGCy'C
Cuts at: 1391 2155 2229 2370 2740 3442 3508 3631 3820
Size: 764 74 141 370 702 66 123 189
Cuts at: 3820 4187 4395 5480 1391
Size: 367 208 1085 4072
Fragments arranged by size:
4072 1085 764 702 370 367 208 189
141 123 74 66
BbeI G_GCGC'C
Cuts at: 3369 4537 3369
Size: 1168 6993
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2975 4066 928
Size: 971 1076 1091 5023
Fragments arranged by size:
5023 1091 1076 971
BbvCI CC'TCA_GC
Cuts at: 2304 4343 2304
Size: 2039 6122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3343 3377 5326 5360 6352 6386 1903
Size: 34 1406 34 1949 34 992 34 3678
Fragments arranged by size:
3678 1949 1406 992 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6053 7263 6053
Size: 1210 6951
BclI T'GATC_A
Cuts at: 1548 2932 3922 1548
Size: 1384 990 5787
Fragments arranged by size:
5787 1384 990
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3626 4930 6772
Size: 107 122 71 631 605 1954 1304 1842
Cuts at: 6772 136
Size: 1525
Fragments arranged by size:
1954 1842 1525 1304 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2997 5217 8156 2997
Size: 2220 2939 3002
Fragments arranged by size:
3002 2939 2220
BlpI GC'TnA_GC
Cuts at: 3101 5040 5578 3101
Size: 1939 538 5684
Fragments arranged by size:
5684 1939 538
Bme1580I G_kGCm'C
Cuts at: 1790 2334 3274 3356 3442 3488 3631 3828 4395
Size: 544 940 82 86 46 143 197 567
Cuts at: 4395 4926 6222 7378 1790
Size: 531 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 940 567 544 531 197
143 86 82 46
BmgBI CAC'GTC
Cuts at: 1318 3400 3616 1318
Size: 2082 216 5863
Fragments arranged by size:
5863 2082 216
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3133 4172 5892
Size: 416 53 83 399 24 1716 1039 1720
Cuts at: 5892 6850 442
Size: 958 1753
Fragments arranged by size:
1753 1720 1716 1039 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 3983 4570 5002 2539
Size: 1444 587 432 5698
Fragments arranged by size:
5698 1444 587 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3728 4490 4775 6821 1239
Size: 2489 762 285 2046 2579
Fragments arranged by size:
2579 2489 2046 762 285
Bpu10I CC'TnA_GC
Cuts at: 2304 3839 4343 2304
Size: 1535 504 6122
Fragments arranged by size:
6122 1535 504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2424 4679 6286 7151 7449 2424
Size: 2255 1607 865 298 3136
Fragments arranged by size:
3136 2255 1607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4133 4810 5279 6824 882
Size: 617 2634 677 469 1545 2219
Fragments arranged by size:
2634 2219 1545 677 617 469
BsaAI yAC'GTr
Cuts at: 493 1204 2355 2938 3829 4995 5952 493
Size: 711 1151 583 891 1166 957 2702
Fragments arranged by size:
2702 1166 1151 957 891 711 583
BsaBI GATnn'nnATC
Cuts at: 8155 8155
Size: 8161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3366 4534 5968 6350
Size: 53 83 186 936 1833 1168 1434 382
Cuts at: 6350 6985 275
Size: 635 1451
Fragments arranged by size:
1833 1451 1434 1168 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2592 4488 6594 6992 7266 7413 7824 2592
Size: 1896 2106 398 274 147 411 2929
Fragments arranged by size:
2929 2106 1896 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5343 5373 1178
Size: 30 165 30 3940 30 3966
Fragments arranged by size:
3966 3940 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3653 4546 4682 4906 1186
Size: 2467 893 136 224 4441
Fragments arranged by size:
4441 2467 893 224 136
BseYI C'CCAG_C
Cuts at: 3583 3846 7364 7860 3583
Size: 263 3518 496 3884
Fragments arranged by size:
3884 3518 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7588
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3009 4054 5611 6372 7400 1540
Size: 275 186 1008 1045 1557 761 1028 2301
Fragments arranged by size:
2301 1557 1045 1028 1008 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2334 3107 3986 4589 5210 6222 6307 7378 2334
Size: 773 879 603 621 1012 85 1071 3117
Fragments arranged by size:
3117 1071 1012 879 773 621 603 85
BsmI GAATG_Cn'
Cuts at: 2752 5672 5765 2752
Size: 2920 93 5148
Fragments arranged by size:
5148 2920 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2229 2334 2370 2740 3107 3274
Size: 399 365 74 105 36 370 367 167
Cuts at: 3274 3356 3442 3488 3508 3631 3820 3828 3986
Size: 82 86 46 20 123 189 8 158
Cuts at: 3986 4187 4395 4589 4926 5210 5480 6222 6307
Size: 201 208 194 337 284 270 742 85
Cuts at: 6307 7378 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 367 365 337
284 270 208 201 194 189 167 158
123 105 86 85 82 74 46 36
20 8
BspEI T'CCGG_A
Cuts at: 2592 7824 2592
Size: 5232 2929
BspHI T'CATG_A
Cuts at: 4362 6050 7780 4362
Size: 1688 1730 4743
Fragments arranged by size:
4743 1730 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2230 5516 844
Size: 1386 3286 3489
Fragments arranged by size:
3489 3286 1386
BsrBI CCG'CTC
Cuts at: 1394 1823 5326 6048 1394
Size: 429 3503 722 3507
Fragments arranged by size:
3507 3503 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2082 2454 4324 6656 6830 65
Size: 1183 834 372 1870 2332 174 1396
Fragments arranged by size:
2332 1870 1396 1183 834 372 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 6805 6992 1700
Size: 110 2952 2043 187 2869
Fragments arranged by size:
2952 2869 2043 187 110
BsrGI T'GTAC_A
Cuts at: 96 5385 96
Size: 5289 2872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8161
BssSI C'ACGA_G
Cuts at: 2928 4379 6221 7233 2928
Size: 1451 1842 1012 3856
Fragments arranged by size:
3856 1842 1451 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3832 4593 794
Size: 3038 761 4362
Fragments arranged by size:
4362 3038 761
BstBI TT'CG_AA
Cuts at: 6965 6965
Size: 8161
BstEII G'GTnAC_C
Cuts at: 1184 4456 4633 4789 4966 1184
Size: 3272 177 156 177 4379
Fragments arranged by size:
4379 3272 177 177 156
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 3752 3895 4909 2143
Size: 1609 143 1014 5395
Fragments arranged by size:
5395 1609 1014 143
BstYI r'GATC_y
Cuts at: 1972 2132 2384 2757 2997 3683 4011 5217 5491
Size: 160 252 373 240 686 328 1206 274
Cuts at: 5491 5858 6243 6260 7712 7798 7810 8156 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1206 686 385 373 367 346
328 274 252 240 160 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2823 3679 2823
Size: 856 7305
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 2688 3393 4798 5058 513
Size: 552 939 243 441 705 1405 260 3616
Fragments arranged by size:
3616 1405 939 705 552 441 260 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2896 3317 5059 487
Size: 683 56 1670 421 1742 3589
Fragments arranged by size:
3589 1742 1670 683 421 56
BtsI GCAGTG_nn'
Cuts at: 2350 2887 3521 5677 6471 6491 2350
Size: 537 634 2156 794 20 4020
Fragments arranged by size:
4020 2156 794 634 537 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8126
DraI TTT'AAA
Cuts at: 5465 5465
Size: 8161
DraIII CAC_nnn'GTG
Cuts at: 3767 3767
Size: 8161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7168 809
Size: 507 5852 1802
Fragments arranged by size:
5852 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 3618 4051
Size: 54 1425 50 275 18 855 933 433
Cuts at: 4051 4250 5608 6497 8126 8
Size: 199 1358 889 1629 43
Fragments arranged by size:
1629 1425 1358 933 889 855 433 275
199 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4051 5608 1537
Size: 275 2239 1557 4090
Fragments arranged by size:
4090 2239 1557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3974 4286 4577 6091 1353
Size: 2621 312 291 1514 3423
Fragments arranged by size:
3423 2621 1514 312 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2222 2521 2689 4277 6748 7122
Size: 1064 138 633 299 168 1588 2471 374
Cuts at: 7122 7268 387
Size: 146 1280
Fragments arranged by size:
2471 1588 1280 1064 633 374 299 168
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2604 2987 3052 3728 4339 4490
Size: 393 479 493 383 65 676 611 151
Cuts at: 4490 4775 6224 6821 7608 7876 1239
Size: 285 1449 597 787 268 1524
Fragments arranged by size:
1524 1449 787 676 611 597 493 479
393 383 285 268 151 65
EcoNI CCTnn'n_nnAGG
Cuts at: 3493 3493
Size: 8161
EcoO109I rG'GnC_Cy
Cuts at: 2242 2422 3378 3438 3439 3627 3628 3778 3843
Size: 180 956 60 1 188 1 150 65
Cuts at: 3843 4174 4392 4575 5053 5274 5308 5438 2242
Size: 331 218 183 478 221 34 130 4965
Fragments arranged by size:
4965 956 478 331 221 218 188 183
180 150 130 65 60 34 1 1
EcoRI G'AATT_C
Cuts at: 2588 4745 5129 5470 5848 2588
Size: 2157 384 341 378 4901
Fragments arranged by size:
4901 2157 384 378 341
EcoRV GAT'ATC
Cuts at: 3281 3281
Size: 8161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 5029 5413 2767
Size: 2262 384 5515
Fragments arranged by size:
5515 2262 384
FspI TGC'GCA
Cuts at: 6667 6667
Size: 8161
HaeII r_GCGC'y
Cuts at: 1678 3075 3369 4519 4537 5014 7308 1678
Size: 1397 294 1150 18 477 2294 2531
Fragments arranged by size:
2531 2294 1397 1150 477 294 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2826 2858 3675
Size: 32 135 32 457 32 765 32 817
Cuts at: 3675 3707 4646 4678 5343 5375 6802 6834 6876
Size: 32 939 32 665 32 1427 32 42
Cuts at: 6876 6908 1373
Size: 32 2626
Fragments arranged by size:
2626 1427 939 817 765 665 457 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 5505 5751 669
Size: 988 281 90 3477 246 3079
Fragments arranged by size:
3477 3079 988 281 246 90
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8161
HpaI GTT'AAC
Cuts at: 2028 5751 2028
Size: 3723 4438
KasI G'GCGC_C
Cuts at: 3365 4533 3365
Size: 1168 6993
KpnI G_GTAC'C
Cuts at: 5486 5486
Size: 8161
MfeI C'AATT_G
Cuts at: 5760 5760
Size: 8161
MluI A'CGCG_T
Cuts at: 8037 8037
Size: 8161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2128 2412 3360 4774 5052 7275 7459 1305
Size: 823 284 948 1414 278 2223 184 2007
Fragments arranged by size:
2223 2007 1414 948 823 284 278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3620 10
Size: 54 1425 343 1788 4551
Fragments arranged by size:
4551 1788 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2861 3176 3323 4565 6119 6478
Size: 648 1191 504 315 147 1242 1554 359
Cuts at: 6478 6637 518
Size: 159 2042
Fragments arranged by size:
2042 1554 1242 1191 648 504 359 315
159 147
MspA1I CmG'CkG
Cuts at: 1606 2308 2665 3712 4593 5213 5577 6254 7402
Size: 702 357 1047 881 620 364 677 1148
Cuts at: 7402 7647 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1047 881 702 677 620 364
357 245
NarI GG'CG_CC
Cuts at: 3366 4534 3366
Size: 1168 6993
NcoI C'CATG_G
Cuts at: 513 1065 2004 4798 513
Size: 552 939 2794 3876
Fragments arranged by size:
3876 2794 939 552
NdeI CA'TA_TG
Cuts at: 387 4949 387
Size: 4562 3599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8161
NotI GC'GGCC_GC
Cuts at: 5608 5608
Size: 8161
NspI r_CATG'y
Cuts at: 1132 1229 2266 3328 4726 5519 7064 1132
Size: 97 1037 1062 1398 793 1545 2229
Fragments arranged by size:
2229 1545 1398 1062 1037 793 97
PasI CC'CwG_GG
Cuts at: 1348 2269 3495 3552 3820 3888 1348
Size: 921 1226 57 268 68 5621
Fragments arranged by size:
5621 1226 921 268 68 57
PciI A'CATG_T
Cuts at: 2262 7060 2262
Size: 4798 3363
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8161
PfoI T'CCnGG_A
Cuts at: 4617 5121 4617
Size: 504 7657
PmeI GTTT'AAAC
Cuts at: 5465 5465
Size: 8161
PmlI CAC'GTG
Cuts at: 2938 3829 2938
Size: 891 7270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4534 6230 6262 7743 7775 4502
Size: 32 1696 32 1481 32 4888
Fragments arranged by size:
4888 1696 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 3778 3843 4174 5274 5308 5438 2242
Size: 1536 65 331 1100 34 130 4965
Fragments arranged by size:
4965 1536 1100 331 130 65 34
PshAI GACnn'nnGTC
Cuts at: 1613 4112 4454 1613
Size: 2499 342 5320
Fragments arranged by size:
5320 2499 342
PsiI TTA'TAA
Cuts at: 5731 5731
Size: 8161
PspOMI G'GGCC_C
Cuts at: 3438 3627 4391 3438
Size: 189 764 7208
Fragments arranged by size:
7208 764 189
PstI C_TGCA'G
Cuts at: 830 2241 4598 5513 830
Size: 1411 2357 915 3478
Fragments arranged by size:
3478 2357 1411 915
PvuI CG_AT'CG
Cuts at: 2001 3009 2001
Size: 1008 7153
PvuII CAG'CTG
Cuts at: 2308 2665 4593 5213 2308
Size: 357 1928 620 5256
Fragments arranged by size:
5256 1928 620 357
SalI G'TCGA_C
Cuts at: 1936 5503 1936
Size: 3567 4594
SanDI GG'GwC_CC
Cuts at: 5438 5438
Size: 8161
SapI GCTCTTCn'nnn_
Cuts at: 3974 4577 3974
Size: 603 7558
SbfI CC_TGCA'GG
Cuts at: 2241 5513 2241
Size: 3272 4889
ScaI AGT'ACT
Cuts at: 2579 3855 6409 8121 2579
Size: 1276 2554 1712 2619
Fragments arranged by size:
2619 2554 1712 1276
SexAI A'CCwGG_T
Cuts at: 3423 3423
Size: 8161
SfcI C'TryA_G
Cuts at: 826 1045 2206 2237 3810 4151 4444 4594 5509
Size: 219 1161 31 1573 341 293 150 915
Cuts at: 5509 6644 7325 7516 826
Size: 1135 681 191 1471
Fragments arranged by size:
1573 1471 1161 1135 915 681 341 293
219 191 150 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 3626 3626
Size: 8161
SfoI GGC'GCC
Cuts at: 3367 4535 3367
Size: 1168 6993
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8161
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8161
SmaI CCC'GGG
Cuts at: 1872 4397 5445 5488 1872
Size: 2525 1048 43 4545
Fragments arranged by size:
4545 2525 1048 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2439 4694 4987 6265 7166 7428
Size: 197 926 496 2255 293 1278 901 262
Cuts at: 7428 820
Size: 1553
Fragments arranged by size:
2255 1553 1278 926 901 496 293 262
197
SnaBI TAC'GTA
Cuts at: 493 5952 493
Size: 5459 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8161
SphI G_CATG'C
Cuts at: 1132 5519 1132
Size: 4387 3774
SspI AAT'ATT
Cuts at: 5 52 6085 5
Size: 47 6033 2081
Fragments arranged by size:
6033 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8161
StyI C'CwwG_G
Cuts at: 513 1065 2004 2799 4675 4798 5004 5600 513
Size: 552 939 795 1876 123 206 596 3074
Fragments arranged by size:
3074 1876 939 795 596 552 206 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3371 4376 6188 6205 6358 6543 6988
Size: 1255 1791 1005 1812 17 153 185 445
Cuts at: 6988 325
Size: 1498
Fragments arranged by size:
1812 1791 1498 1255 1005 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 3853 5385
Size: 274 80 33 51 1443 600 1276 1532
Cuts at: 5385 6407 8119 96
Size: 1022 1712 138
Fragments arranged by size:
1712 1532 1443 1276 1022 600 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 8121 8153 2124
Size: 32 5965 32 2132
Fragments arranged by size:
5965 2132 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3425 1316
Size: 2109 6052
XbaI T'CTAG_A
Cuts at: 5497 5497
Size: 8161
XhoI C'TCGA_G
Cuts at: 1943 4987 1943
Size: 3044 5117
XmaI C'CCGG_G
Cuts at: 1870 4395 5443 5486 1870
Size: 2525 1048 43 4545
Fragments arranged by size:
4545 2525 1048 43
XmnI GAAnn'nnTTC
Cuts at: 6290 6290
Size: 8161
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5969 276
Size: 53 83 186 936 4435 2468
Fragments arranged by size:
4435 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacI SacII SrfI SwaI
XcmI