Restriction map for
pFN21AB3039
(Circular) (Six-base) MAPSORT of: pFN21AB3039.seq Check: 319 from: 1 to: 8077
pFN21AB3039 8077 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2141 2544 2141
Size: 403 7674
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5887 278
Size: 53 83 186 936 4351 2468
Fragments arranged by size:
4351 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5420 1937
Size: 3483 4594
Acc65I G'GTAC_C
Cuts at: 5398 5398
Size: 8077
AclI AA'CG_TT
Cuts at: 1563 6204 6577 1563
Size: 4641 373 3063
Fragments arranged by size:
4641 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5385 6140 7524 7792 1632
Size: 3753 755 1384 268 1917
Fragments arranged by size:
3753 1917 1384 755 268
AflII C'TTAA_G
Cuts at: 820 1017 4520 820
Size: 197 3503 4377
Fragments arranged by size:
4377 3503 197
AflIII A'CryG_T
Cuts at: 1203 2753 2778 6976 7953 1203
Size: 1550 25 4198 977 1327
Fragments arranged by size:
4198 1550 1327 977 25
AgeI A'CCGG_T
Cuts at: 6908 6908
Size: 8077
AhdI GACnn_n'nnGTC
Cuts at: 6806 6806
Size: 8077
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8077
AlwNI CAG_nnn'CTG
Cuts at: 3293 5820 7392 3293
Size: 2527 1572 3978
Fragments arranged by size:
3978 2527 1572
ApaLI G'TGCA_C
Cuts at: 6134 7290 6134
Size: 1156 6921
ApoI r'AATT_y
Cuts at: 1493 3098 3682 4478 5386 5612 5764 7873 1493
Size: 1605 584 796 908 226 152 2109 1697
Fragments arranged by size:
2109 1697 1605 908 796 584 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6631 160
Size: 872 5599 1606
Fragments arranged by size:
5599 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8077
AvaI C'yCGr_G
Cuts at: 1870 1943 4851 4962 5402 1870
Size: 73 2908 111 440 4545
Fragments arranged by size:
4545 2908 440 111 73
BamHI G'GATC_C
Cuts at: 2212 3243 5407 5774 2212
Size: 1031 2164 367 4515
Fragments arranged by size:
4515 2164 1031 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2151 5398 6853 618
Size: 325 217 627 282 82 3247 1455 1842
Fragments arranged by size:
3247 1842 1455 627 325 282 217 82
BanII G_rGCy'C
Cuts at: 1391 2166 3018 5396 1391
Size: 775 852 2378 4072
Fragments arranged by size:
4072 2378 852 775
BbeI G_GCGC'C
Cuts at: 2155 2155
Size: 8077
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4053 928
Size: 971 2154 4952
Fragments arranged by size:
4952 2154 971
BbvCI CC'TCA_GC
Cuts at: 3964 3964
Size: 8077
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6268 6302 1903
Size: 34 4331 34 3678
Fragments arranged by size:
4331 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2334 5969 7179 2334
Size: 3635 1210 3232
Fragments arranged by size:
3635 3232 1210
BclI T'GATC_A
Cuts at: 1548 2088 3763 4305 4597 1548
Size: 540 1675 542 292 5028
Fragments arranged by size:
5028 1675 542 540 292
BfrBI ATG'CAT
Cuts at: 2724 2724
Size: 8077
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4847 6688 136
Size: 107 122 71 631 605 3175 1841 1525
Fragments arranged by size:
3175 1841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3886 8072 3886
Size: 4186 3891
BlpI GC'TnA_GC
Cuts at: 2547 5494 2547
Size: 2947 5130
Bme1580I G_kGCm'C
Cuts at: 1790 2502 6138 7294 1790
Size: 712 3636 1156 2573
Fragments arranged by size:
3636 2573 1156 712
BmgBI CAC'GTC
Cuts at: 1318 2754 4846 1318
Size: 1436 2092 4549
Fragments arranged by size:
4549 2092 1436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5000 5808 6766
Size: 416 53 83 399 24 3583 808 958
Cuts at: 6766 442
Size: 1753
Fragments arranged by size:
3583 1753 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8077
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2612 2612
Size: 8077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2583 2736 2985 3002 4784 5195 6737 1239
Size: 1344 153 249 17 1782 411 1542 2579
Fragments arranged by size:
2579 1782 1542 1344 411 249 153 17
Bpu10I CC'TnA_GC
Cuts at: 2942 3964 5104 2942
Size: 1022 1140 5915
Fragments arranged by size:
5915 1140 1022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2214 4085 4194 4888 6202 7067 7365 2214
Size: 1871 109 694 1314 865 298 2926
Fragments arranged by size:
2926 1871 1314 865 694 298 109
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6740 882
Size: 617 5241 2219
Fragments arranged by size:
5241 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3838 5311 5868 493
Size: 711 2634 1473 557 2702
Fragments arranged by size:
2702 2634 1473 711 557
BsaBI GATnn'nnATC
Cuts at: 2087 5021 8071 2087
Size: 2934 3050 2093
Fragments arranged by size:
3050 2934 2093
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2152 5884 6266 6901
Size: 53 83 186 936 619 3732 382 635
Cuts at: 6901 275
Size: 1451
Fragments arranged by size:
3732 1451 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2628 3240 6510 6908 7182 7329 7740 2628
Size: 612 3270 398 274 147 411 2965
Fragments arranged by size:
3270 2965 612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2403 2433 3255 3285 3731
Size: 30 165 30 1000 30 822 30 446
Cuts at: 3731 3761 1178
Size: 30 5494
Fragments arranged by size:
5494 1000 822 446 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2340 3627 3912 3952 5309 1186
Size: 1154 1287 285 40 1357 3954
Fragments arranged by size:
3954 1357 1287 1154 285 40
BseYI C'CCAG_C
Cuts at: 3293 4171 4774 4818 7280 7776 3293
Size: 878 603 44 2462 496 3594
Fragments arranged by size:
3594 2462 878 603 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2759 2827 2968 4406 5157 1163
Size: 573 1023 68 141 1438 751 4083
Fragments arranged by size:
4083 1438 1023 751 573 141 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5376 5527 6288 7316 1540
Size: 275 186 3375 151 761 1028 2301
Fragments arranged by size:
3375 2301 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 3183 6138 6223 7294 3183
Size: 2955 85 1071 3966
Fragments arranged by size:
3966 2955 1071 85
BsmI GAATG_Cn'
Cuts at: 2666 2726 3355 4081 5588 5681 2666
Size: 60 629 726 1507 93 5062
Fragments arranged by size:
5062 1507 726 629 93 60
BsmBI CGTCTCn'nnnn_
Cuts at: 3598 4760 3598
Size: 1162 6915
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2502 3018 3183 5396 6138 6223
Size: 399 376 336 516 165 2213 742 85
Cuts at: 6223 7294 1391
Size: 1071 2174
Fragments arranged by size:
2213 2174 1071 742 516 399 376 336
165 85
BspEI T'CCGG_A
Cuts at: 2628 7740 2628
Size: 5112 2965
BspHI T'CATG_A
Cuts at: 4183 5966 7696 4183
Size: 1783 1730 4564
Fragments arranged by size:
4564 1783 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 2544 4286 5432 844
Size: 1297 403 1742 1146 3489
Fragments arranged by size:
3489 1742 1297 1146 403
BsrBI CCG'CTC
Cuts at: 1394 1823 5964 1394
Size: 429 4141 3507
Fragments arranged by size:
4141 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2136 2905 4785 6572 6746 65
Size: 1183 888 769 1880 1787 174 1396
Fragments arranged by size:
1880 1787 1396 1183 888 769 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6721 6908 1700
Size: 110 4911 187 2869
Fragments arranged by size:
4911 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5046 96
Size: 4950 3127
BssHII G'CGCG_C
Cuts at: 1925 4526 1925
Size: 2601 5476
BssSI C'ACGA_G
Cuts at: 6137 7149 6137
Size: 1012 7065
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8077
BstBI TT'CG_AA
Cuts at: 6881 6881
Size: 8077
BstEII G'GTnAC_C
Cuts at: 1184 4192 1184
Size: 3008 5069
BstXI CCAn_nnnn'nTGG
Cuts at: 2561 5013 2561
Size: 2452 5625
BstYI r'GATC_y
Cuts at: 1972 2212 3243 3886 4438 4894 5407 5774 6159
Size: 240 1031 643 552 456 513 367 385
Cuts at: 6159 6176 7628 7714 7726 8072 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1031 643 552 513 456 385
367 346 240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 4723 513
Size: 552 939 69 2650 3867
Fragments arranged by size:
3867 2650 939 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7338
Fragments arranged by size:
7338 683 56
BtsI GCAGTG_nn'
Cuts at: 2469 2815 2900 3283 3891 5593 6387 6407 2469
Size: 346 85 383 608 1702 794 20 4139
Fragments arranged by size:
4139 1702 794 608 383 346 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3042 3077 529
Size: 35 2478 35 5529
Fragments arranged by size:
5529 2478 35 35
DraI TTT'AAA
Cuts at: 5381 5381
Size: 8077
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7084 809
Size: 507 5768 1802
Fragments arranged by size:
5768 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2574 3128 4510
Size: 54 1425 50 275 18 744 554 1382
Cuts at: 4510 4704 5019 5373 5524 6413 8042 8
Size: 194 315 354 151 889 1629 43
Fragments arranged by size:
1629 1425 1382 889 744 554 354 315
275 194 151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5373 5524 1537
Size: 275 3561 151 4090
Fragments arranged by size:
4090 3561 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 2603 4316 6007 1353
Size: 911 339 1713 1691 3423
Fragments arranged by size:
3423 1713 1691 911 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6664 7038 7184 387
Size: 1064 138 5075 374 146 1280
Fragments arranged by size:
5075 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2583 2736 2985 3002 4784 5195 5385
Size: 393 951 153 249 17 1782 411 190
Cuts at: 5385 6140 6737 7524 7792 1239
Size: 755 597 787 268 1524
Fragments arranged by size:
1782 1524 951 787 755 597 411 393
268 249 190 153 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2614 3258 3642 2614
Size: 644 384 7049
Fragments arranged by size:
7049 644 384
EcoO109I rG'GnC_Cy
Cuts at: 2654 4105 2654
Size: 1451 6626
EcoRI G'AATT_C
Cuts at: 5386 5764 5386
Size: 378 7699
FspI TGC'GCA
Cuts at: 6583 6583
Size: 8077
HaeII r_GCGC'y
Cuts at: 1678 2155 7224 1678
Size: 477 5069 2531
Fragments arranged by size:
5069 2531 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2319 2351 2959 2991 3200
Size: 32 135 32 747 32 608 32 209
Cuts at: 3200 3232 3255 3287 3731 3763 3837 3869 3871
Size: 32 23 32 444 32 74 32 2
Cuts at: 3871 3903 5054 5086 5237 5269 6718 6750 6792
Size: 32 1151 32 151 32 1449 32 42
Cuts at: 6792 6824 1373
Size: 32 2626
Fragments arranged by size:
2626 1449 1151 747 608 444 209 151
135 74 42 32 32 32 32 32
32 32 32 32 32 32 32 32
23 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2363 3856 5421 5667 669
Size: 988 281 425 1493 1565 246 3079
Fragments arranged by size:
3079 1565 1493 988 425 281 246
HindIII A'AGCT_T
Cuts at: 748 2367 2939 3117 3443 748
Size: 1619 572 178 326 5382
Fragments arranged by size:
5382 1619 572 326 178
HpaI GTT'AAC
Cuts at: 5667 5667
Size: 8077
KasI G'GCGC_C
Cuts at: 2151 2151
Size: 8077
KpnI G_GTAC'C
Cuts at: 5402 5402
Size: 8077
MfeI C'AATT_G
Cuts at: 4316 5676 4316
Size: 1360 6717
MluI A'CGCG_T
Cuts at: 7953 7953
Size: 8077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3972 7191 7375 1305
Size: 2667 3219 184 2007
Fragments arranged by size:
3219 2667 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4512 4706 5021 10
Size: 54 1425 343 2680 194 315 3066
Fragments arranged by size:
3066 2680 1425 343 315 194 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 4059 6035 6394 6553 518
Size: 648 1188 1705 1976 359 159 2042
Fragments arranged by size:
2042 1976 1705 1188 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2218 2952 3968 4220 4382 5493 6170 7318
Size: 612 734 1016 252 162 1111 677 1148
Cuts at: 7318 7563 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1111 1016 734 677 612 252
245 162
NarI GG'CG_CC
Cuts at: 2152 2152
Size: 8077
NcoI C'CATG_G
Cuts at: 513 1065 2004 2073 4723 513
Size: 552 939 69 2650 3867
Fragments arranged by size:
3867 2650 939 552 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8077
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8077
NotI GC'GGCC_GC
Cuts at: 5524 5524
Size: 8077
NsiI A_TGCA'T
Cuts at: 2726 2726
Size: 8077
NspI r_CATG'y
Cuts at: 1132 1229 2910 4680 5435 6980 1132
Size: 97 1681 1770 755 1545 2229
Fragments arranged by size:
2229 1770 1681 1545 755 97
PasI CC'CwG_GG
Cuts at: 1348 3825 3868 5161 1348
Size: 2477 43 1293 4264
Fragments arranged by size:
4264 2477 1293 43
PciI A'CATG_T
Cuts at: 6976 6976
Size: 8077
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2247 2880 5269 1108
Size: 1139 633 2389 3916
Fragments arranged by size:
3916 2389 1139 633
PfoI T'CCnGG_A
Cuts at: 3001 3592 3001
Size: 591 7486
PmeI GTTT'AAAC
Cuts at: 5381 5381
Size: 8077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6146 6178 7659 7691 6146
Size: 32 1481 32 6532
Fragments arranged by size:
6532 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2654 2654
Size: 8077
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8077
PsiI TTA'TAA
Cuts at: 5647 5647
Size: 8077
PstI C_TGCA'G
Cuts at: 830 2895 3278 5429 830
Size: 2065 383 2151 3478
Fragments arranged by size:
3478 2151 2065 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8077
PvuII CAG'CTG
Cuts at: 2952 3968 4382 2952
Size: 1016 414 6647
Fragments arranged by size:
6647 1016 414
SalI G'TCGA_C
Cuts at: 1936 5419 1936
Size: 3483 4594
SbfI CC_TGCA'GG
Cuts at: 5429 5429
Size: 8077
ScaI AGT'ACT
Cuts at: 6325 8037 6325
Size: 1712 6365
SexAI A'CCwGG_T
Cuts at: 4128 4128
Size: 8077
SfcI C'TryA_G
Cuts at: 826 1045 2891 3274 5167 5425 6560 7241 7432
Size: 219 1846 383 1893 258 1135 681 191
Cuts at: 7432 826
Size: 1471
Fragments arranged by size:
1893 1846 1471 1135 681 383 258 219
191
SfoI GGC'GCC
Cuts at: 2153 2153
Size: 8077
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8077
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8077
SmaI CCC'GGG
Cuts at: 1872 4853 5404 1872
Size: 2981 551 4545
Fragments arranged by size:
4545 2981 551
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 4100 4209 4520 4867 6181
Size: 197 926 286 1871 109 311 347 1314
Cuts at: 6181 7082 7344 820
Size: 901 262 1553
Fragments arranged by size:
1871 1553 1314 926 901 347 311 286
262 197 109
SnaBI TAC'GTA
Cuts at: 493 5868 493
Size: 5375 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8077
SphI G_CATG'C
Cuts at: 1132 2910 5435 1132
Size: 1778 2525 3774
Fragments arranged by size:
3774 2525 1778
SspI AAT'ATT
Cuts at: 5 52 3702 3775 3987 6001 5
Size: 47 3650 73 212 2014 2081
Fragments arranged by size:
3650 2081 2014 212 73 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8077
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2983 3861 4258 4723 5516
Size: 552 939 69 910 878 397 465 793
Cuts at: 5516 513
Size: 3074
Fragments arranged by size:
3074 939 910 878 793 552 465 397
69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2136 2850 6104 6121 6274 6459 6904
Size: 1255 556 714 3254 17 153 185 445
Cuts at: 6904 325
Size: 1498
Fragments arranged by size:
3254 1498 1255 714 556 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2530 2568 2675
Size: 274 80 33 51 1443 553 38 107
Cuts at: 2675 5046 6323 8035 96
Size: 2371 1277 1712 138
Fragments arranged by size:
2371 1712 1443 1277 553 274 138 107
80 51 38 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 3892 3924 5119 5151 8037 8069 2403
Size: 32 1457 32 1195 32 2886 32 2411
Fragments arranged by size:
2886 2411 1457 1195 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8077
XbaI T'CTAG_A
Cuts at: 2524 5413 2524
Size: 2889 5188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 4461 2711
Size: 1750 6327
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8077
XmaI C'CCGG_G
Cuts at: 1870 4851 5402 1870
Size: 2981 551 4545
Fragments arranged by size:
4545 2981 551
XmnI GAAnn'nnTTC
Cuts at: 6206 6206
Size: 8077
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5885 276
Size: 53 83 186 936 4351 2468
Fragments arranged by size:
4351 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI