Restriction map for
pFN21AB3038
(Circular) (Six-base) MAPSORT of: pFN21AB3038.seq Check: 3916 from: 1 to: 8908
pFN21AB3038 8908 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6718 278
Size: 53 83 186 936 5182 2468
Fragments arranged by size:
5182 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 3229 6251 1937
Size: 484 808 3022 4594
Fragments arranged by size:
4594 3022 808 484
Acc65I G'GTAC_C
Cuts at: 6229 6229
Size: 8908
AclI AA'CG_TT
Cuts at: 1563 7035 7408 1563
Size: 5472 373 3063
Fragments arranged by size:
5472 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 4149 4449 4715 5535 5924 6971 8355
Size: 654 1863 300 266 820 389 1047 1384
Cuts at: 8355 8623 1632
Size: 268 1917
Fragments arranged by size:
1917 1863 1384 1047 820 654 389 300
268 266
AflII C'TTAA_G
Cuts at: 820 1017 4934 820
Size: 197 3917 4794
Fragments arranged by size:
4794 3917 197
AflIII A'CryG_T
Cuts at: 1203 7807 8784 1203
Size: 6604 977 1327
Fragments arranged by size:
6604 1327 977
AgeI A'CCGG_T
Cuts at: 7739 7739
Size: 8908
AhdI GACnn_n'nnGTC
Cuts at: 2554 7637 2554
Size: 5083 3825
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8908
AlwNI CAG_nnn'CTG
Cuts at: 3481 3620 4277 5675 6651 8223 3481
Size: 139 657 1398 976 1572 4166
Fragments arranged by size:
4166 1572 1398 976 657 139
ApaLI G'TGCA_C
Cuts at: 6087 6965 8121 6087
Size: 878 1156 6874
Fragments arranged by size:
6874 1156 878
ApoI r'AATT_y
Cuts at: 1493 2508 2622 3570 5563 6217 6443 6595 8704
Size: 1015 114 948 1993 654 226 152 2109
Cuts at: 8704 1493
Size: 1697
Fragments arranged by size:
2109 1993 1697 1015 948 654 226 152
114
AseI AT'TA_AT
Cuts at: 160 1032 4566 7462 160
Size: 872 3534 2896 1606
Fragments arranged by size:
3534 2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8908
AvaI C'yCGr_G
Cuts at: 1870 1943 6233 1870
Size: 73 4290 4545
Fragments arranged by size:
4545 4290 73
AvrII C'CTAG_G
Cuts at: 3076 3076
Size: 8908
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2350 2383 2350
Size: 33 8875
BamHI G'GATC_C
Cuts at: 5041 6238 6605 5041
Size: 1197 367 7344
Fragments arranged by size:
7344 1197 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 3311 4183 6229 7684
Size: 325 217 627 414 1110 872 2046 1455
Cuts at: 7684 618
Size: 1842
Fragments arranged by size:
2046 1842 1455 1110 872 627 414 325
217
BanII G_rGCy'C
Cuts at: 1391 4598 4933 6227 1391
Size: 3207 335 1294 4072
Fragments arranged by size:
4072 3207 1294 335
BbeI G_GCGC'C
Cuts at: 2205 2205
Size: 8908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5882 928
Size: 971 3983 3954
Fragments arranged by size:
3983 3954 971
BbvCI CC'TCA_GC
Cuts at: 4338 4338
Size: 8908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7099 7133 1903
Size: 34 5162 34 3678
Fragments arranged by size:
5162 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6800 8010 6800
Size: 1210 7698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8908
BfrBI ATG'CAT
Cuts at: 2438 2761 2438
Size: 323 8585
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7519 136
Size: 107 122 71 631 605 5847 1525
Fragments arranged by size:
5847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2773 4609 5084 5599 8903 2773
Size: 1836 475 515 3304 2778
Fragments arranged by size:
3304 2778 1836 515 475
BlpI GC'TnA_GC
Cuts at: 4681 6325 4681
Size: 1644 7264
Bme1580I G_kGCm'C
Cuts at: 1790 6091 6969 8125 1790
Size: 4301 878 1156 2573
Fragments arranged by size:
4301 2573 1156 878
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2231 4002 5047
Size: 416 53 83 399 24 814 1771 1045
Cuts at: 5047 5533 6639 7597 442
Size: 486 1106 958 1753
Fragments arranged by size:
1771 1753 1106 1045 958 814 486 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8908
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2042 3316 5946 2042
Size: 1274 2630 5004
Fragments arranged by size:
5004 2630 1274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3286 3363 3693 3995 4227 4947 5667 7568
Size: 2047 77 330 302 232 720 720 1901
Cuts at: 7568 1239
Size: 2579
Fragments arranged by size:
2579 2047 1901 720 720 330 302 232
77
Bpu10I CC'TnA_GC
Cuts at: 4338 4338
Size: 8908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7033 7898 8196 7033
Size: 865 298 7745
Fragments arranged by size:
7745 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3529 5857 7571 882
Size: 617 2030 2328 1714 2219
Fragments arranged by size:
2328 2219 2030 1714 617
BsaAI yAC'GTr
Cuts at: 493 1204 4916 6699 493
Size: 711 3712 1783 2702
Fragments arranged by size:
3712 2702 1783 711
BsaBI GATnn'nnATC
Cuts at: 8902 8902
Size: 8908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 2202 6715 7097
Size: 53 83 186 936 581 88 4513 382
Cuts at: 7097 7732 275
Size: 635 1451
Fragments arranged by size:
4513 1451 936 635 581 382 186 88
83 53
BsaWI w'CCGG_w
Cuts at: 7341 7739 8013 8160 8571 7341
Size: 398 274 147 411 7678
Fragments arranged by size:
7678 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4474 4504 5108 5138 5640
Size: 30 165 30 3071 30 604 30 502
Cuts at: 5640 5670 6166 6196 1178
Size: 30 496 30 3890
Fragments arranged by size:
3890 3071 604 502 496 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2150 3278 3905 4093 5116 5236 5239
Size: 937 27 1128 627 188 1023 120 3
Cuts at: 5239 6174 1186
Size: 935 3920
Fragments arranged by size:
3920 1128 1023 937 935 627 188 120
27 3
BseYI C'CCAG_C
Cuts at: 2120 2486 3757 4002 4417 4676 8111 8607 2120
Size: 366 1271 245 415 259 3435 496 2421
Fragments arranged by size:
3435 2421 1271 496 415 366 259 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2587 2703 2976 3153 1163
Size: 573 851 116 273 177 6918
Fragments arranged by size:
6918 851 573 273 177 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6358 7119 8147 1540
Size: 275 186 4357 761 1028 2301
Fragments arranged by size:
4357 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4933 6091 6969 7054 8125 4933
Size: 1158 878 85 1071 5716
Fragments arranged by size:
5716 1158 1071 878 85
BsmI GAATG_Cn'
Cuts at: 2395 6419 6512 2395
Size: 4024 93 4791
Fragments arranged by size:
4791 4024 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3667 3667
Size: 8908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4598 4933 6091 6227 6969 7054 8125
Size: 399 2808 335 1158 136 742 85 1071
Cuts at: 8125 1391
Size: 2174
Fragments arranged by size:
2808 2174 1158 1071 742 399 335 136
85
BspEI T'CCGG_A
Cuts at: 8571 8571
Size: 8908
BspHI T'CATG_A
Cuts at: 2191 6797 8527 2191
Size: 4606 1730 2572
Fragments arranged by size:
4606 2572 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3840 3950 6263 844
Size: 2996 110 2313 3489
Fragments arranged by size:
3489 2996 2313 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2097 6795 1394
Size: 429 274 4698 3507
Fragments arranged by size:
4698 3507 429 274
BsrDI GCAATG_nn'
Cuts at: 65 1248 2634 5712 5915 5934 6073 7403 7577
Size: 1183 1386 3078 203 19 139 1330 174
Cuts at: 7577 65
Size: 1396
Fragments arranged by size:
3078 1396 1386 1330 1183 203 174 139
19
BsrFI r'CCGG_y
Cuts at: 1700 1810 7552 7739 1700
Size: 110 5742 187 2869
Fragments arranged by size:
5742 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3037 4883 96
Size: 2941 1846 4121
Fragments arranged by size:
4121 2941 1846
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8908
BssSI C'ACGA_G
Cuts at: 6968 7980 6968
Size: 1012 7896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2345 794
Size: 1551 7357
BstBI TT'CG_AA
Cuts at: 7712 7712
Size: 8908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8908
BstXI CCAn_nnnn'nTGG
Cuts at: 4019 4019
Size: 8908
BstYI r'GATC_y
Cuts at: 1972 2773 2842 4609 5041 5084 5599 5857 6238
Size: 801 69 1767 432 43 515 258 381
Cuts at: 6238 6605 6990 7007 8459 8545 8557 8903 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1767 1452 801 515 432 385 381
367 346 258 86 69 43 17 12
Bsu36I CC'TnA_GG
Cuts at: 3108 4120 3108
Size: 1012 7896
BtgI C'CryG_G
Cuts at: 513 1065 3006 3049 3896 4294 5811 513
Size: 552 1941 43 847 398 1517 3610
Fragments arranged by size:
3610 1941 1517 847 552 398 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8169
Fragments arranged by size:
8169 683 56
BtsI GCAGTG_nn'
Cuts at: 3599 4110 4224 5766 6424 7218 7238 3599
Size: 511 114 1542 658 794 20 5269
Fragments arranged by size:
5269 1542 794 658 511 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3334 3369 529
Size: 35 2770 35 6068
Fragments arranged by size:
6068 2770 35 35
DraI TTT'AAA
Cuts at: 2565 3465 5242 6212 2565
Size: 900 1777 970 5261
Fragments arranged by size:
5261 1777 970 900
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7915 809
Size: 507 6599 1802
Fragments arranged by size:
6599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2854 3052 3622
Size: 54 1425 50 275 18 1024 198 570
Cuts at: 3622 3705 6355 7244 8873 8
Size: 83 2650 889 1629 43
Fragments arranged by size:
2650 1629 1425 1024 889 570 275 198
83 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6355 1537
Size: 275 4543 4090
Fragments arranged by size:
4543 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3991 4459 6838 1353
Size: 2638 468 2379 3423
Fragments arranged by size:
3423 2638 2379 468
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2169 5983 6187 7495 7869 8015
Size: 1064 138 580 3814 204 1308 374 146
Cuts at: 8015 387
Size: 1280
Fragments arranged by size:
3814 1308 1280 1064 580 374 204 146
138
EcoICRI GAG'CTC
Cuts at: 4931 4931
Size: 8908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 3286 3363 3693 3995 4149 4227
Size: 393 654 1000 77 330 302 154 78
Cuts at: 4227 4449 4715 4947 5535 5667 5924 6971 7568
Size: 222 266 232 588 132 257 1047 597
Cuts at: 7568 8355 8623 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1047 1000 787 654 597 588 393
330 302 268 266 257 232 222 154
132 78 77
EcoO109I rG'GnC_Cy
Cuts at: 2215 3962 5234 6148 2215
Size: 1747 1272 914 4975
Fragments arranged by size:
4975 1747 1272 914
EcoRI G'AATT_C
Cuts at: 3570 6217 6595 3570
Size: 2647 378 5883
Fragments arranged by size:
5883 2647 378
FspI TGC'GCA
Cuts at: 7414 7414
Size: 8908
HaeII r_GCGC'y
Cuts at: 1678 2150 2205 8055 1678
Size: 472 55 5850 2531
Fragments arranged by size:
5850 2531 472 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2540 2572 2650
Size: 32 135 32 527 32 409 32 78
Cuts at: 2650 2682 2695 2727 4292 4324 5180 5212 5640
Size: 32 13 32 1565 32 856 32 428
Cuts at: 5640 5672 7549 7581 7623 7655 1373
Size: 32 1877 32 42 32 2626
Fragments arranged by size:
2626 1877 1565 856 527 428 409 135
78 42 32 32 32 32 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2590 4624 5470 6252 6498 669
Size: 988 281 652 2034 846 782 246 3079
Fragments arranged by size:
3079 2034 988 846 782 652 281 246
HindIII A'AGCT_T
Cuts at: 748 2682 5864 748
Size: 1934 3182 3792
Fragments arranged by size:
3792 3182 1934
HpaI GTT'AAC
Cuts at: 4624 5470 6498 4624
Size: 846 1028 7034
Fragments arranged by size:
7034 1028 846
KasI G'GCGC_C
Cuts at: 2201 2201
Size: 8908
KpnI G_GTAC'C
Cuts at: 6233 6233
Size: 8908
MfeI C'AATT_G
Cuts at: 3041 3346 5097 6507 3041
Size: 305 1751 1410 5442
Fragments arranged by size:
5442 1751 1410 305
MluI A'CGCG_T
Cuts at: 8784 8784
Size: 8908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2735 4728 8022 8206 1305
Size: 1430 1993 3294 184 2007
Fragments arranged by size:
3294 2007 1993 1430 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2856 3054 3624 3707 10
Size: 54 1425 343 1024 198 570 83 5211
Fragments arranged by size:
5211 1425 1024 570 343 198 83 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 2433 2443 2646 3054 6866 7225
Size: 648 1030 237 10 203 408 3812 359
Cuts at: 7225 7384 518
Size: 159 2042
Fragments arranged by size:
3812 2042 1030 648 408 359 237 203
159 10
MspA1I CmG'CkG
Cuts at: 1606 2042 3496 3590 4019 4421 6324 7001 8149
Size: 436 1454 94 429 402 1903 677 1148
Cuts at: 8149 8394 1606
Size: 245 2120
Fragments arranged by size:
2120 1903 1454 1148 677 436 429 402
245 94
NarI GG'CG_CC
Cuts at: 2202 2202
Size: 8908
NcoI C'CATG_G
Cuts at: 513 1065 3006 3049 4294 5811 513
Size: 552 1941 43 1245 1517 3610
Fragments arranged by size:
3610 1941 1517 1245 552 43
NdeI CA'TA_TG
Cuts at: 387 4992 387
Size: 4605 4303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8908
NotI GC'GGCC_GC
Cuts at: 6355 6355
Size: 8908
NsiI A_TGCA'T
Cuts at: 2440 2763 2440
Size: 323 8585
NspI r_CATG'y
Cuts at: 1132 1229 3365 4704 6266 7811 1132
Size: 97 2136 1339 1562 1545 2229
Fragments arranged by size:
2229 2136 1562 1545 1339 97
PasI CC'CwG_GG
Cuts at: 1348 3692 1348
Size: 2344 6564
PciI A'CATG_T
Cuts at: 7807 7807
Size: 8908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8908
PfoI T'CCnGG_A
Cuts at: 5133 5825 5133
Size: 692 8216
PmeI GTTT'AAAC
Cuts at: 6212 6212
Size: 8908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3966 3998 6977 7009 8490 8522 3966
Size: 32 2979 32 1481 32 4352
Fragments arranged by size:
4352 2979 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3962 5234 6148 3962
Size: 1272 914 6722
Fragments arranged by size:
6722 1272 914
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8908
PsiI TTA'TAA
Cuts at: 2433 6478 2433
Size: 4045 4863
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5799 5831 5799
Size: 32 8876
PstI C_TGCA'G
Cuts at: 830 3064 3727 3837 5944 6260 830
Size: 2234 663 110 2107 316 3478
Fragments arranged by size:
3478 2234 2107 663 316 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8908
PvuII CAG'CTG
Cuts at: 3496 3590 4019 4421 3496
Size: 94 429 402 7983
Fragments arranged by size:
7983 429 402 94
SacI G_AGCT'C
Cuts at: 4933 4933
Size: 8908
SalI G'TCGA_C
Cuts at: 1936 6250 1936
Size: 4314 4594
SbfI CC_TGCA'GG
Cuts at: 6260 6260
Size: 8908
ScaI AGT'ACT
Cuts at: 7156 8868 7156
Size: 1712 7196
SexAI A'CCwGG_T
Cuts at: 4312 5434 4312
Size: 1122 7786
SfcI C'TryA_G
Cuts at: 826 1045 2362 2769 3060 3230 3723 3739 3833
Size: 219 1317 407 291 170 493 16 94
Cuts at: 3833 5940 5995 6256 7391 8072 8263 826
Size: 2107 55 261 1135 681 191 1471
Fragments arranged by size:
2107 1471 1317 1135 681 493 407 291
261 219 191 170 94 55 16
SfoI GGC'GCC
Cuts at: 2203 2203
Size: 8908
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8908
SmaI CCC'GGG
Cuts at: 1872 6235 1872
Size: 4363 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4934 7012 7913 8175 820
Size: 197 926 2991 2078 901 262 1553
Fragments arranged by size:
2991 2078 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6699 493
Size: 6206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8908
SphI G_CATG'C
Cuts at: 1132 4704 6266 1132
Size: 3572 1562 3774
Fragments arranged by size:
3774 3572 1562
SspI AAT'ATT
Cuts at: 5 52 5266 5479 6832 5
Size: 47 5214 213 1353 2081
Fragments arranged by size:
5214 2081 1353 213 47
StuI AGG'CCT
Cuts at: 1517 3023 5123 1517
Size: 1506 2100 5302
Fragments arranged by size:
5302 2100 1506
StyI C'CwwG_G
Cuts at: 513 1065 2502 2796 2832 3006 3049 3076 4294
Size: 552 1437 294 36 174 43 27 1218
Cuts at: 4294 5811 6347 513
Size: 1517 536 3074
Fragments arranged by size:
3074 1517 1437 1218 552 536 294 174
43 36 27
SwaI ATTT'AAAT
Cuts at: 3465 3465
Size: 8908
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4431 6935 6952 7105 7290 7735 325
Size: 1255 2851 2504 17 153 185 445 1498
Fragments arranged by size:
2851 2504 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3037 3382 3764
Size: 274 80 33 51 1443 1060 345 382
Cuts at: 3764 4883 6070 6135 7154 8866 96
Size: 1119 1187 65 1019 1712 138
Fragments arranged by size:
1712 1443 1187 1119 1060 1019 382 345
274 138 80 65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3351 3383 8868 8900 3351
Size: 32 5485 32 3359
Fragments arranged by size:
5485 3359 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4400 1316
Size: 3084 5824
XbaI T'CTAG_A
Cuts at: 6244 6244
Size: 8908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4375 4375
Size: 8908
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8908
XmaI C'CCGG_G
Cuts at: 1870 6233 1870
Size: 4363 4545
XmnI GAAnn'nnTTC
Cuts at: 5540 7037 5540
Size: 1497 7411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6716 276
Size: 53 83 186 936 5182 2468
Fragments arranged by size:
5182 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BsiWI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI
SfiI SrfI