Restriction map for
pFN21AB3031
(Circular) (Six-base) MAPSORT of: pFN21AB3031.seq Check: 110 from: 1 to: 6484
pFN21AB3031 6484 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4547 5931 6199 1632
Size: 2915 1384 268 1917
Fragments arranged by size:
2915 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 5383 6360 1203
Size: 4180 977 1327
Fragments arranged by size:
4180 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 2494 5213 2494
Size: 2719 3765
AleI CACnn'nnGTG
Cuts at: 1166 2042 2886 3381 1166
Size: 876 844 495 4269
Fragments arranged by size:
4269 876 844 495
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3399 1184
Size: 2215 4269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 2430
Size: 32 6452
AlwNI CAG_nnn'CTG
Cuts at: 2533 4227 5799 2533
Size: 1694 1572 3218
Fragments arranged by size:
3218 1694 1572
ApaLI G'TGCA_C
Cuts at: 3329 3374 4541 5697 3329
Size: 45 1167 1156 4116
Fragments arranged by size:
4116 1167 1156 45
ApoI r'AATT_y
Cuts at: 1493 2213 2753 2942 3793 4019 4171 6280 1493
Size: 720 540 189 851 226 152 2109 1697
Fragments arranged by size:
2109 1697 851 720 540 226 189 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3054 3809 1870
Size: 73 1111 755 4545
Fragments arranged by size:
4545 1111 755 73
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3805 5260 618
Size: 325 217 627 2018 1455 1842
Fragments arranged by size:
2018 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3680 3803 1391
Size: 2289 123 4072
Fragments arranged by size:
4072 2289 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3732 928
Size: 971 1833 3680
Fragments arranged by size:
3680 1833 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 2860 1548
Size: 1312 5172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2225 5095 136
Size: 107 122 71 631 605 553 2870 1525
Fragments arranged by size:
2870 1525 631 605 553 122 107 71
BglII A'GATC_T
Cuts at: 2578 6479 2578
Size: 3901 2583
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 3291 3333 3378 3624 4545 5701 1790
Size: 1501 42 45 246 921 1156 2573
Fragments arranged by size:
2573 1501 1156 921 246 45 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4215 5173 442
Size: 416 53 83 399 24 2798 958 1753
Fragments arranged by size:
2798 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 2457 3341 5144 1239
Size: 900 318 884 1803 2579
Fragments arranged by size:
2579 1803 900 884 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 4609 5474 5772 2624
Size: 1985 865 298 3336
Fragments arranged by size:
3336 1985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2711 3504 5147 882
Size: 617 1212 793 1643 2219
Fragments arranged by size:
2219 1643 1212 793 617
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 2889 6478 2889
Size: 3589 2895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3147 4291 4673 5308
Size: 53 83 186 936 1614 1144 382 635
Cuts at: 5308 275
Size: 1451
Fragments arranged by size:
1614 1451 1144 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2430 2460 1178
Size: 30 165 30 1027 30 5202
Fragments arranged by size:
5202 1027 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2088 2568 3338 1186
Size: 902 480 770 4332
Fragments arranged by size:
4332 902 770 480
BseYI C'CCAG_C
Cuts at: 3290 5687 6183 3290
Size: 2397 496 3591
Fragments arranged by size:
3591 2397 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2337 3732 1163
Size: 573 601 1395 3915
Fragments arranged by size:
3915 1395 601 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3369 3934 4695 5723 1540
Size: 275 186 1368 565 761 1028 2301
Fragments arranged by size:
2301 1368 1028 761 565 275 186
BsiHKAI G_wGCw'C
Cuts at: 3333 3378 4545 4630 5701 3333
Size: 45 1167 85 1071 4116
Fragments arranged by size:
4116 1167 1071 85 45
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3291 3333 3378 3624 3680 3803 4545
Size: 399 1501 42 45 246 56 123 742
Cuts at: 4545 4630 5701 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1501 1071 742 399 246 123 85
56 45 42
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 3066 3184 4373 6103 3066
Size: 118 1189 1730 3447
Fragments arranged by size:
3447 1730 1189 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3839 844
Size: 2995 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3182 4371 1394
Size: 429 1359 1189 3507
Fragments arranged by size:
3507 1359 1189 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2491 2985 4979 5153 65
Size: 1183 1243 494 1994 174 1396
Fragments arranged by size:
1994 1396 1243 1183 494 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3364 5128 5315 1700
Size: 110 1554 1764 187 2869
Fragments arranged by size:
2869 1764 1554 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 3490 3665 3490
Size: 175 6309
BstYI r'GATC_y
Cuts at: 1972 2320 2578 3814 4181 4566 4583 6035 6121
Size: 348 258 1236 367 385 17 1452 86
Cuts at: 6121 6133 6479 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1236 385 367 348 346 258
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3280 513
Size: 552 2215 3717
Fragments arranged by size:
3717 2215 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 1995 487
Size: 683 56 769 4976
Fragments arranged by size:
4976 769 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 3388 4000 4794 4814 3376
Size: 12 612 794 20 5046
Fragments arranged by size:
5046 794 612 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 3286 3710 3286
Size: 424 6060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3272 3394 3931
Size: 54 1425 50 275 18 1442 122 537
Cuts at: 3931 4820 6449 8
Size: 889 1629 43
Fragments arranged by size:
1629 1442 1425 889 537 275 122 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3591 4414 1353
Size: 2238 823 3423
Fragments arranged by size:
3423 2238 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3046 5071 5445 5591 387
Size: 1064 138 1457 2025 374 146 1280
Fragments arranged by size:
2025 1457 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2457 3341 4547 5144 5931 6199
Size: 393 507 318 884 1206 597 787 268
Cuts at: 6199 1239
Size: 1524
Fragments arranged by size:
1524 1206 884 787 597 507 393 318
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2332 3323 2332
Size: 991 5493
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FspI TGC'GCA
Cuts at: 2459 4990 2459
Size: 2531 3953
FspAI rTGC'GCAy
Cuts at: 2459 2459
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 3180 5631 1678
Size: 1502 2451 2531
Fragments arranged by size:
2531 2451 1502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 2480 2509 2512
Size: 32 135 32 740 32 136 29 3
Cuts at: 2512 2541 3048 3080 3655 3687 5125 5157 5199
Size: 29 507 32 575 32 1438 32 42
Cuts at: 5199 5231 1373
Size: 32 2626
Fragments arranged by size:
2626 1438 740 575 507 136 135 42
32 32 32 32 32 32 32 29
29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2067 3457 3828 4074 669
Size: 988 281 129 1390 371 246 3079
Fragments arranged by size:
3079 1390 988 371 281 246 129
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3202 3722 5598 5782 1305
Size: 1897 520 1876 184 2007
Fragments arranged by size:
2007 1897 1876 520 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4662
Fragments arranged by size:
4662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2886 3381 3488 4442 4801 4960
Size: 648 876 844 495 107 954 359 159
Cuts at: 4960 518
Size: 2042
Fragments arranged by size:
2042 954 876 844 648 495 359 159
107
MspA1I CmG'CkG
Cuts at: 1606 3900 4577 5725 5970 1606
Size: 2294 677 1148 245 2120
Fragments arranged by size:
2294 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5932
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 3842 5387 1132
Size: 97 2613 1545 2229
Fragments arranged by size:
2613 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3299 1348
Size: 1951 4533
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6484
PfoI T'CCnGG_A
Cuts at: 3318 3318
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2430 2462 4553 4585 6066 6098 2430
Size: 32 2091 32 1481 32 2816
Fragments arranged by size:
2816 2091 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 2595 4054 2595
Size: 1459 5025
PstI C_TGCA'G
Cuts at: 830 3383 3836 830
Size: 2553 453 3478
Fragments arranged by size:
3478 2553 453
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SapI GCTCTTCn'nnn_
Cuts at: 3591 3591
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 2631 4732 6444 2631
Size: 2101 1712 2671
Fragments arranged by size:
2671 2101 1712
SfcI C'TryA_G
Cuts at: 826 1045 2791 3379 3578 3832 4967 5648 5839
Size: 219 1746 588 199 254 1135 681 191
Cuts at: 5839 826
Size: 1471
Fragments arranged by size:
1746 1471 1135 681 588 254 219 199
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 4588 5489 5751 820
Size: 197 926 696 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 696 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 3637 4408 5
Size: 47 3585 771 2081
Fragments arranged by size:
3585 2081 771 47
StuI AGG'CCT
Cuts at: 1517 2101 1517
Size: 584 5900
StyI C'CwwG_G
Cuts at: 513 1065 3923 513
Size: 552 2858 3074
Fragments arranged by size:
3074 2858 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2043 2174 4511 4528 4681 4866 5311
Size: 1255 463 131 2337 17 153 185 445
Cuts at: 5311 325
Size: 1498
Fragments arranged by size:
2337 1498 1255 463 445 185 153 131
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2629 3692 4730
Size: 274 80 33 51 1443 652 1063 1038
Cuts at: 4730 6442 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1063 1038 652 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6444 6476 6444
Size: 32 6452
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2432 2432
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 4613 3776
Size: 837 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI