Restriction map for
pFN21AB3029
(Circular) (Six-base) MAPSORT of: pFN21AB3029.seq Check: 7904 from: 1 to: 6736
pFN21AB3029 6736 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2589 2589
Size: 6736
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3794 4079 1937
Size: 1857 285 4594
Fragments arranged by size:
4594 1857 285
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2713 3312 4799 6183 6451 1632
Size: 1081 599 1487 1384 268 1917
Fragments arranged by size:
1917 1487 1384 1081 599 268
AfeI AGC'GCT
Cuts at: 2700 2700
Size: 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 5635 6612 1203
Size: 4432 977 1327
Fragments arranged by size:
4432 1327 977
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 3070 5465 3070
Size: 2395 4341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6736
AlwNI CAG_nnn'CTG
Cuts at: 2260 4479 6051 2260
Size: 2219 1572 2945
Fragments arranged by size:
2945 2219 1572
ApaI G_GGCC'C
Cuts at: 3903 3903
Size: 6736
ApaLI G'TGCA_C
Cuts at: 2077 4793 5949 2077
Size: 2716 1156 2864
Fragments arranged by size:
2864 2716 1156
ApoI r'AATT_y
Cuts at: 1493 2588 2801 4045 4271 4423 6532 1493
Size: 1095 213 1244 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 1095 226 213 152
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 2680 3200 3686 4061 1870
Size: 73 737 520 486 375 4545
Fragments arranged by size:
4545 737 520 486 375 73
BamHI G'GATC_C
Cuts at: 4066 4433 4066
Size: 367 6369
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 2364 2673 3167 4057
Size: 325 217 627 487 90 309 494 890
Cuts at: 4057 5512 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 890 627 494 487 325 309
217 90
BanII G_rGCy'C
Cuts at: 1391 2526 3159 3498 3903 3966 4055 1391
Size: 1135 633 339 405 63 89 4072
Fragments arranged by size:
4072 1135 633 405 339 89 63
BbeI G_GCGC'C
Cuts at: 2278 2677 2278
Size: 399 6337
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2814 928
Size: 971 915 4850
Fragments arranged by size:
4850 971 915
BbvCI CC'TCA_GC
Cuts at: 2459 3641 2459
Size: 1182 5554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2026 2060 4927 4961 1903
Size: 34 89 34 2867 34 3678
Fragments arranged by size:
3678 2867 89 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6736
BfrBI ATG'CAT
Cuts at: 2722 3294 2722
Size: 572 6164
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2682 3041 3572
Size: 107 122 71 631 605 1010 359 531
Cuts at: 3572 3871 5347 136
Size: 299 1476 1525
Fragments arranged by size:
1525 1476 1010 631 605 531 359 299
122 107 71
BglII A'GATC_T
Cuts at: 3603 6731 3603
Size: 3128 3608
BlpI GC'TnA_GC
Cuts at: 3861 4153 3861
Size: 292 6444
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2438 3172 3903 3979 4797 5953 1790
Size: 291 357 734 731 76 818 1156 2573
Fragments arranged by size:
2573 1156 818 734 731 357 291 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4467 5425 442
Size: 416 53 83 399 24 3050 958 1753
Fragments arranged by size:
3050 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2717
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2456 2879 2966 3389 3592 3611 5396
Size: 881 336 423 87 423 203 19 1785
Cuts at: 5396 1239
Size: 2579
Fragments arranged by size:
2579 1785 881 423 423 336 203 87
19
Bpu10I CC'TnA_GC
Cuts at: 2459 3641 2459
Size: 1182 5554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4861 5726 6024 4861
Size: 865 298 5573
Fragments arranged by size:
5573 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5399 882
Size: 617 3900 2219
Fragments arranged by size:
3900 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2227 2395 2488 4527 493
Size: 711 1023 168 93 2039 2702
Fragments arranged by size:
2702 2039 1023 711 168 93
BsaBI GATnn'nnATC
Cuts at: 6730 6730
Size: 6736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2275 2674 3728 4543
Size: 53 83 186 936 742 399 1054 815
Cuts at: 4543 4925 5560 275
Size: 382 635 1451
Fragments arranged by size:
1451 1054 936 815 742 635 399 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5169 5567 5841 5988 6399 5169
Size: 398 274 147 411 5506
Fragments arranged by size:
5506 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2852 2882 1178
Size: 30 165 30 1449 30 5032
Fragments arranged by size:
5032 1449 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2876 2879 2963 3104 3122 3289 3938
Size: 960 730 3 84 141 18 167 649
Cuts at: 3938 3950 3953 3965 1186
Size: 12 3 12 3957
Fragments arranged by size:
3957 960 730 649 167 141 84 18
12 12 3 3
BseYI C'CCAG_C
Cuts at: 2891 5939 6435 2891
Size: 3048 496 3192
Fragments arranged by size:
3192 3048 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 1163
Size: 573 411 5752
Fragments arranged by size:
5752 573 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2163 2380 4186 4947 5975 1540
Size: 275 186 162 217 1806 761 1028 2301
Fragments arranged by size:
2301 1806 1028 761 275 217 186 162
BsiHKAI G_wGCw'C
Cuts at: 2081 2526 3159 3498 3633 4797 4882 5953 2081
Size: 445 633 339 135 1164 85 1071 2864
Fragments arranged by size:
2864 1164 1071 633 445 339 135 85
BsiWI C'GTAC_G
Cuts at: 2426 2426
Size: 6736
BsmI GAATG_Cn'
Cuts at: 2724 3296 4247 4340 2724
Size: 572 951 93 5120
Fragments arranged by size:
5120 951 572 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3327 3327
Size: 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2438 2526 3159 3172 3498 3633
Size: 399 291 357 88 633 13 326 135
Cuts at: 3633 3903 3966 3979 4055 4797 4882 5953 1391
Size: 270 63 13 76 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 633 399 357 326 291
270 135 88 85 76 63 13 13
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 2844 4625 6355 2844
Size: 1781 1730 3225
Fragments arranged by size:
3225 1781 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 2589 3456 4091 844
Size: 1631 114 867 635 3489
Fragments arranged by size:
3489 1631 867 635 114
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 4623 1394
Size: 429 2074 726 3507
Fragments arranged by size:
3507 2074 726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5231 5405 65
Size: 1183 3983 174 1396
Fragments arranged by size:
3983 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2539 2670 3009 3650 3730 5380 5567
Size: 110 729 131 339 641 80 1650 187
Cuts at: 5567 1700
Size: 2869
Fragments arranged by size:
2869 1650 729 641 339 187 131 110
80
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6736
BssHII G'CGCG_C
Cuts at: 1925 2060 1925
Size: 135 6601
BssSI C'ACGA_G
Cuts at: 2606 4796 5808 2606
Size: 2190 1012 3534
Fragments arranged by size:
3534 2190 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2085 2260 794
Size: 1291 175 5270
Fragments arranged by size:
5270 1291 175
BstBI TT'CG_AA
Cuts at: 5540 5540
Size: 6736
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6736
BstYI r'GATC_y
Cuts at: 1972 3603 4066 4433 4818 4835 6287 6373 6385
Size: 1631 463 367 385 17 1452 86 12
Cuts at: 6385 6731 1972
Size: 346 1977
Fragments arranged by size:
1977 1631 1452 463 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2627 2627
Size: 6736
BtgI C'CryG_G
Cuts at: 513 1065 2023 2163 2374 3120 3819 513
Size: 552 958 140 211 746 699 3430
Fragments arranged by size:
3430 958 746 699 552 211 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4032 487
Size: 683 56 2806 3191
Fragments arranged by size:
3191 2806 683 56
BtsI GCAGTG_nn'
Cuts at: 2317 3252 4252 5046 5066 2317
Size: 935 1000 794 20 3987
Fragments arranged by size:
3987 1000 935 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3795 3830 529
Size: 35 3231 35 3435
Fragments arranged by size:
3435 3231 35 35
DraI TTT'AAA
Cuts at: 4040 4040
Size: 6736
DraIII CAC_nnn'GTG
Cuts at: 2611 2611
Size: 6736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2352 2377
Size: 54 1425 50 275 18 330 192 25
Cuts at: 2377 2541 3011 3033 3123 3732 3816 4183 5072
Size: 164 470 22 90 609 84 367 889
Cuts at: 5072 6701 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 609 470 367 330 275
192 164 90 84 54 50 43 25
22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2160 2377 4183 1537
Size: 275 348 217 1806 4090
Fragments arranged by size:
4090 1806 348 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2783 4666 1353
Size: 1430 1883 3423
Fragments arranged by size:
3423 1883 1430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5323 5697 5843 387
Size: 1064 138 3734 374 146 1280
Fragments arranged by size:
3734 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 3157 3496 2524
Size: 633 339 5764
Fragments arranged by size:
5764 633 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2456 2713 2879 2966 3312 3389
Size: 393 488 336 257 166 87 346 77
Cuts at: 3389 3592 3611 4799 5396 6183 6451 1239
Size: 203 19 1188 597 787 268 1524
Fragments arranged by size:
1524 1188 787 597 488 393 346 336
268 257 203 166 87 77 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2861 2471
Size: 390 6346
EcoO109I rG'GnC_Cy
Cuts at: 3028 3231 3637 3873 3028
Size: 203 406 236 5891
Fragments arranged by size:
5891 406 236 203
EcoRI G'AATT_C
Cuts at: 2588 4045 4423 2588
Size: 1457 378 4901
Fragments arranged by size:
4901 1457 378
FspI TGC'GCA
Cuts at: 2577 5242 2577
Size: 2665 4071
HaeII r_GCGC'y
Cuts at: 1678 2278 2677 2702 3651 3699 5883 1678
Size: 600 399 25 949 48 2184 2531
Fragments arranged by size:
2531 2184 949 600 399 48 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2864 2896 3056 3088 5377
Size: 32 135 32 1292 32 160 32 2289
Cuts at: 5377 5409 5451 5483 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2289 1292 160 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 4080 4326 669
Size: 988 281 891 1251 246 3079
Fragments arranged by size:
3079 1251 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6736
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KasI G'GCGC_C
Cuts at: 2274 2673 2274
Size: 399 6337
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 2985 4335 2985
Size: 1350 5386
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3378 5850 6034 1305
Size: 2073 2472 184 2007
Fragments arranged by size:
2472 2073 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3035 3818 10
Size: 54 1425 343 1203 783 2928
Fragments arranged by size:
2928 1425 1203 783 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2756 4694 5053 5212 518
Size: 648 948 642 1938 359 159 2042
Fragments arranged by size:
2042 1938 948 648 642 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2145 2302 2308 2376 2891 3122 4152
Size: 419 120 157 6 68 515 231 1030
Cuts at: 4152 4829 5977 6222 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1030 677 515 419 245 231
157 120 68 6
NaeI GCC'GGC
Cuts at: 2541 2672 3011 3652 3732 2541
Size: 131 339 641 80 5545
Fragments arranged by size:
5545 641 339 131 80
NarI GG'CG_CC
Cuts at: 2275 2674 2275
Size: 399 6337
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6184
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6736
NgoMIV G'CCGG_C
Cuts at: 2539 2670 3009 3650 3730 2539
Size: 131 339 641 80 5545
Fragments arranged by size:
5545 641 339 131 80
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NsiI A_TGCA'T
Cuts at: 2724 3296 2724
Size: 572 6164
NspI r_CATG'y
Cuts at: 1132 1229 2449 4094 5639 1132
Size: 97 1220 1645 1545 2229
Fragments arranged by size:
2229 1645 1545 1220 97
PasI CC'CwG_GG
Cuts at: 1348 3226 1348
Size: 1878 4858
PciI A'CATG_T
Cuts at: 5635 5635
Size: 6736
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2290 1108
Size: 1182 5554
PfoI T'CCnGG_A
Cuts at: 2451 2451
Size: 6736
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PmlI CAC'GTG
Cuts at: 2227 2395 2227
Size: 168 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2114 2146 4805 4837 6318 6350 2114
Size: 32 2659 32 1481 32 2500
Fragments arranged by size:
2659 2500 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3231 3231
Size: 6736
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PspOMI G'GGCC_C
Cuts at: 3899 3899
Size: 6736
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 6704
PstI C_TGCA'G
Cuts at: 830 2265 2307 3259 3984 4088 830
Size: 1435 42 952 725 104 3478
Fragments arranged by size:
3478 1435 952 725 104 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2302 2308 2891 2302
Size: 6 583 6147
Fragments arranged by size:
6147 583 6
SacI G_AGCT'C
Cuts at: 2526 3159 3498 2526
Size: 633 339 5764
Fragments arranged by size:
5764 633 339
SacII CC_GC'GG
Cuts at: 2026 2377 3123 2026
Size: 351 746 5639
Fragments arranged by size:
5639 746 351
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SanDI GG'GwC_CC
Cuts at: 3231 3231
Size: 6736
SbfI CC_TGCA'GG
Cuts at: 4088 4088
Size: 6736
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SexAI A'CCwGG_T
Cuts at: 2415 2415
Size: 6736
SfcI C'TryA_G
Cuts at: 826 1045 2261 2303 2636 3255 3980 4084 5219
Size: 219 1216 42 333 619 725 104 1135
Cuts at: 5219 5900 6091 826
Size: 681 191 1471
Fragments arranged by size:
1471 1216 1135 725 681 619 333 219
191 104 42
SfoI GGC'GCC
Cuts at: 2276 2675 2276
Size: 399 6337
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 3650 1700
Size: 1950 4786
SmaI CCC'GGG
Cuts at: 1872 4063 1872
Size: 2191 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4840 5741 6003 820
Size: 197 926 2897 901 262 1553
Fragments arranged by size:
2897 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4527 493
Size: 4034 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6736
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SspI AAT'ATT
Cuts at: 5 52 4660 5
Size: 47 4608 2081
Fragments arranged by size:
4608 2081 47
StuI AGG'CCT
Cuts at: 1517 3784 1517
Size: 2267 4469
StyI C'CwwG_G
Cuts at: 513 1065 3021 3160 4175 513
Size: 552 1956 139 1015 3074
Fragments arranged by size:
3074 1956 1015 552 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 4763 4780 4933 5118 5563 325
Size: 1255 621 2562 17 153 185 445 1498
Fragments arranged by size:
2562 1498 1255 621 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4982 6694 96
Size: 274 80 33 51 1443 3005 1712 138
Fragments arranged by size:
3005 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 2597 2629 2852 2884 6696 6728 2060
Size: 32 505 32 223 32 3812 32 2068
Fragments arranged by size:
3812 2068 505 223 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3028 3028
Size: 6736
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 4061 1870
Size: 2191 4545
XmnI GAAnn'nnTTC
Cuts at: 4865 4865
Size: 6736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BstXI BstZ17I EcoRV FalI
FseI FspAI NruI PacI PspXI RsrII SapI SfiI
SrfI SwaI