Restriction map for
pFN21AB3028
(Circular) (Six-base) MAPSORT of: pFN21AB3028.seq Check: 3816 from: 1 to: 8257
pFN21AB3028 8257 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3774 4349 5538 3774
Size: 575 1189 6493
Fragments arranged by size:
6493 1189 575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6067 278
Size: 53 83 186 936 4531 2468
Fragments arranged by size:
4531 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2926 5600 1937
Size: 989 2674 4594
Fragments arranged by size:
4594 2674 989
Acc65I G'GTAC_C
Cuts at: 2681 5578 2681
Size: 2897 5360
AclI AA'CG_TT
Cuts at: 1563 6384 6757 1563
Size: 4821 373 3063
Fragments arranged by size:
4821 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2872 3187 4664 5088 6320 7704 7972 1632
Size: 1240 315 1477 424 1232 1384 268 1917
Fragments arranged by size:
1917 1477 1384 1240 1232 424 315 268
AfeI AGC'GCT
Cuts at: 5375 5375
Size: 8257
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8060
AflIII A'CryG_T
Cuts at: 1203 3234 7156 8133 1203
Size: 2031 3922 977 1327
Fragments arranged by size:
3922 2031 1327 977
AgeI A'CCGG_T
Cuts at: 7088 7088
Size: 8257
AhdI GACnn_n'nnGTC
Cuts at: 6986 6986
Size: 8257
AleI CACnn'nnGTG
Cuts at: 1166 4097 1166
Size: 2931 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4166 4590 5498 1184
Size: 2982 424 908 3943
Fragments arranged by size:
3943 2982 908 424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 8225
AlwNI CAG_nnn'CTG
Cuts at: 2161 2428 2816 4798 4900 6000 7572 2161
Size: 267 388 1982 102 1100 1572 2846
Fragments arranged by size:
2846 1982 1572 1100 388 267 102
ApaI G_GGCC'C
Cuts at: 2567 3803 4788 2567
Size: 1236 985 6036
Fragments arranged by size:
6036 1236 985
ApaLI G'TGCA_C
Cuts at: 6314 7470 6314
Size: 1156 7101
ApoI r'AATT_y
Cuts at: 1493 5566 5792 5944 8053 1493
Size: 4073 226 152 2109 1697
Fragments arranged by size:
4073 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3477 6811 160
Size: 872 2445 3334 1606
Fragments arranged by size:
3334 2445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8257
AvaI C'yCGr_G
Cuts at: 1870 1943 3886 4065 4581 4601 5582 1870
Size: 73 1943 179 516 20 981 4545
Fragments arranged by size:
4545 1943 981 516 179 73 20
BamHI G'GATC_C
Cuts at: 2308 2463 5062 5587 5954 2308
Size: 155 2599 525 367 4611
Fragments arranged by size:
4611 2599 525 367 155
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2681 3772 5287 5550 5578
Size: 325 217 627 894 1091 1515 263 28
Cuts at: 5578 7033 618
Size: 1455 1842
Fragments arranged by size:
1842 1515 1455 1091 894 627 325 263
217 28
BanII G_rGCy'C
Cuts at: 1391 2482 2567 3803 3906 4072 4608 4788 4860
Size: 1091 85 1236 103 166 536 180 72
Cuts at: 4860 5303 5348 5576 1391
Size: 443 45 228 4072
Fragments arranged by size:
4072 1236 1091 536 443 228 180 166
103 85 72 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6448 6482 1903
Size: 34 4511 34 3678
Fragments arranged by size:
4511 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6149 7359 6149
Size: 1210 7047
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3807 4297 5177
Size: 107 122 71 631 605 2135 490 880
Cuts at: 5177 5307 5405 6868 136
Size: 130 98 1463 1525
Fragments arranged by size:
2135 1525 1463 880 631 605 490 130
122 107 98 71
BglII A'GATC_T
Cuts at: 8252 8252
Size: 8257
BlpI GC'TnA_GC
Cuts at: 2261 3182 3341 3713 5153 5297 5674 2261
Size: 921 159 372 1440 144 377 4844
Fragments arranged by size:
4844 1440 921 377 372 159 144
Bme1580I G_kGCm'C
Cuts at: 1790 2567 3777 3803 3812 4788 6318 7474 1790
Size: 777 1210 26 9 976 1530 1156 2573
Fragments arranged by size:
2573 1530 1210 1156 976 777 26 9
BmgBI CAC'GTC
Cuts at: 1318 3064 1318
Size: 1746 6511
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 2526 3506
Size: 416 53 83 399 24 754 355 980
Cuts at: 3506 5988 6946 442
Size: 2482 958 1753
Fragments arranged by size:
2482 1753 980 958 754 416 399 355
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2466 3923 5095 5362 5416 2464
Size: 2 1457 1172 267 54 5305
Fragments arranged by size:
5305 1457 1172 267 54 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2853 3299 4043 4698 5043 5362 5375 6917
Size: 1614 446 744 655 345 319 13 1542
Cuts at: 6917 1239
Size: 2579
Fragments arranged by size:
2579 1614 1542 744 655 446 345 319
13
Bpu10I CC'TnA_GC
Cuts at: 4346 4346
Size: 8257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 6382 7247 7545 4159
Size: 2223 865 298 4871
Fragments arranged by size:
4871 2223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 4674 6920 882
Size: 617 1068 2107 2246 2219
Fragments arranged by size:
2246 2219 2107 1068 617
BsaAI yAC'GTr
Cuts at: 493 1204 6048 493
Size: 711 4844 2702
Fragments arranged by size:
4844 2702 711
BsaBI GATnn'nnATC
Cuts at: 8251 8251
Size: 8257
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6064 6446 7081 275
Size: 53 83 186 936 4531 382 635 1451
Fragments arranged by size:
4531 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6690 7088 7362 7509 7920 6690
Size: 398 274 147 411 7027
Fragments arranged by size:
7027 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2512 2542 2828 2858 4256
Size: 30 165 30 1109 30 286 30 1398
Cuts at: 4256 4286 1178
Size: 30 5149
Fragments arranged by size:
5149 1398 1109 286 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 4711 4863 4868 5264 1186
Size: 1000 2525 152 5 396 4179
Fragments arranged by size:
4179 2525 1000 396 152 5
BseYI C'CCAG_C
Cuts at: 2029 2189 3001 3078 3193 3802 4425 4608 4893
Size: 160 812 77 115 609 623 183 285
Cuts at: 4893 5126 7460 7956 2029
Size: 233 2334 496 2330
Fragments arranged by size:
2334 2330 812 623 609 496 285 233
183 160 115 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2495 2760 5484 1163
Size: 573 759 265 2724 3936
Fragments arranged by size:
3936 2724 759 573 265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5707 6468 7496 1540
Size: 275 186 3706 761 1028 2301
Fragments arranged by size:
3706 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2482 2541 3714 4347 4507 4860 5348 6318 6403
Size: 59 1173 633 160 353 488 970 85
Cuts at: 6403 7474 2482
Size: 1071 3265
Fragments arranged by size:
3265 1173 1071 970 633 488 353 160
85 59
BsmI GAATG_Cn'
Cuts at: 3345 3754 4579 5768 5861 3345
Size: 409 825 1189 93 5741
Fragments arranged by size:
5741 1189 825 409 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4729 4729
Size: 8257
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2482 2541 2567 3714 3777 3803 3812
Size: 399 692 59 26 1147 63 26 9
Cuts at: 3812 3906 4072 4347 4507 4608 4788 4860 5303
Size: 94 166 275 160 101 180 72 443
Cuts at: 5303 5348 5576 6318 6403 7474 1391
Size: 45 228 742 85 1071 2174
Fragments arranged by size:
2174 1147 1071 742 692 443 399 275
228 180 166 160 101 94 85 72
63 59 45 26 26 9
BspEI T'CCGG_A
Cuts at: 7920 7920
Size: 8257
BspHI T'CATG_A
Cuts at: 3457 6146 7876 3457
Size: 2689 1730 3838
Fragments arranged by size:
3838 2689 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3384 3774 4349 5538 5612 844
Size: 2540 390 575 1189 74 3489
Fragments arranged by size:
3489 2540 1189 575 390 74
BsrBI CCG'CTC
Cuts at: 1394 1823 4840 6144 1394
Size: 429 3017 1304 3507
Fragments arranged by size:
3507 3017 1304 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 3928 6752 6926 65
Size: 1183 1759 921 2824 174 1396
Fragments arranged by size:
2824 1759 1396 1183 921 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3590 4507 5148 6901 7088 1700
Size: 110 1780 917 641 1753 187 2869
Fragments arranged by size:
2869 1780 1753 917 641 187 110
BsrGI T'GTAC_A
Cuts at: 96 4470 96
Size: 4374 3883
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8257
BssSI C'ACGA_G
Cuts at: 4334 5311 6317 7329 4334
Size: 977 1006 1012 5262
Fragments arranged by size:
5262 1012 1006 977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2017 2026 3634 3878 5550 794
Size: 1223 9 1608 244 1672 3501
Fragments arranged by size:
3501 1672 1608 1223 244 9
BstBI TT'CG_AA
Cuts at: 7061 7061
Size: 8257
BstEII G'GTnAC_C
Cuts at: 1184 2083 1184
Size: 899 7358
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 3796 2239
Size: 1557 6700
BstYI r'GATC_y
Cuts at: 1972 2308 2370 2463 4794 5062 5587 5954 6339
Size: 336 62 93 2331 268 525 367 385
Cuts at: 6339 6356 7808 7894 7906 8252 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2331 1977 1452 525 385 367 346 336
268 93 86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 2220 4109 5470 2220
Size: 1889 1361 5007
Fragments arranged by size:
5007 1889 1361
BtgI C'CryG_G
Cuts at: 513 1065 2402 4848 5244 5388 513
Size: 552 1337 2446 396 144 3382
Fragments arranged by size:
3382 2446 1337 552 396 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3732 4424 487
Size: 683 56 2506 692 4320
Fragments arranged by size:
4320 2506 692 683 56
BtsI GCAGTG_nn'
Cuts at: 3172 3275 3555 4696 5348 5773 6567 6587 3172
Size: 103 280 1141 652 425 794 20 4842
Fragments arranged by size:
4842 1141 794 652 425 280 103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3849 3884 529
Size: 35 3285 35 4902
Fragments arranged by size:
4902 3285 35 35
DraI TTT'AAA
Cuts at: 5561 5561
Size: 8257
DraIII CAC_nnn'GTG
Cuts at: 4336 4336
Size: 8257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7264 809
Size: 507 5948 1802
Fragments arranged by size:
5948 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3588 3828 3845
Size: 54 1425 50 275 18 1758 240 17
Cuts at: 3845 4962 5319 5454 5704 6593 8222 8
Size: 1117 357 135 250 889 1629 43
Fragments arranged by size:
1758 1629 1425 1117 889 357 275 250
240 135 54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 5704 1537
Size: 275 3892 4090
Fragments arranged by size:
4090 3892 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3108 3438 3576 3947 5064 5097 6187 1353
Size: 1755 330 138 371 1117 33 1090 3423
Fragments arranged by size:
3423 1755 1117 1090 371 330 138 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2194 4526 6844 7218 7364 387
Size: 1064 138 605 2332 2318 374 146 1280
Fragments arranged by size:
2332 2318 1280 1064 605 374 146 138
EcoICRI GAG'CTC
Cuts at: 2480 4858 5346 2480
Size: 2378 488 5391
Fragments arranged by size:
5391 2378 488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2853 2872 3187 3299 4043 4664 4698
Size: 393 1221 19 315 112 744 621 34
Cuts at: 4698 5043 5088 5362 5375 6320 6917 7704 7972
Size: 345 45 274 13 945 597 787 268
Cuts at: 7972 1239
Size: 1524
Fragments arranged by size:
1524 1221 945 787 744 621 597 393
345 315 274 268 112 45 34 19
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2820 4617 2820
Size: 1797 6460
EcoO109I rG'GnC_Cy
Cuts at: 2498 2564 2825 2980 2987 4134 4248 4299 4429
Size: 66 261 155 7 1147 114 51 130
Cuts at: 4429 4444 4784 4785 5054 5074 5259 5436 5491
Size: 15 340 1 269 20 185 177 55
Cuts at: 5491 2498
Size: 5264
Fragments arranged by size:
5264 1147 340 269 261 185 177 155
130 114 66 55 51 20 15 7
1
EcoRI G'AATT_C
Cuts at: 5566 5944 5566
Size: 378 7879
FspI TGC'GCA
Cuts at: 6763 6763
Size: 8257
HaeII r_GCGC'y
Cuts at: 1678 2228 2448 5160 5377 5411 7404 1678
Size: 550 220 2712 217 34 1993 2531
Fragments arranged by size:
2712 2531 1993 550 220 217 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3869 3901 4256 4288 4763
Size: 32 135 32 2297 32 355 32 475
Cuts at: 4763 4795 6898 6930 6972 7004 1373
Size: 32 2103 32 42 32 2626
Fragments arranged by size:
2626 2297 2103 475 355 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 2701 2927 4543 5601 5847
Size: 988 281 708 55 226 1616 1058 246
Cuts at: 5847 669
Size: 3079
Fragments arranged by size:
3079 1616 1058 988 708 281 246 226
55
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8257
HpaI GTT'AAC
Cuts at: 2646 5847 2646
Size: 3201 5056
KpnI G_GTAC'C
Cuts at: 2685 5582 2685
Size: 2897 5360
MfeI C'AATT_G
Cuts at: 5856 5856
Size: 8257
MluI A'CGCG_T
Cuts at: 8133 8133
Size: 8257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3564 7371 7555 1305
Size: 2259 3807 184 2007
Fragments arranged by size:
3807 2259 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3830 4964 5321 5456 10
Size: 54 1425 343 1998 1134 357 135 2811
Fragments arranged by size:
2811 1998 1425 1134 357 343 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2597 3233 4097 4148 4392 4752
Size: 648 1071 360 636 864 51 244 360
Cuts at: 4752 5387 5480 6215 6574 6733 518
Size: 635 93 735 359 159 2042
Fragments arranged by size:
2042 1071 864 735 648 636 635 360
360 359 244 159 93 51
MspA1I CmG'CkG
Cuts at: 1606 2215 2293 3624 3806 3970 4897 5673 6350
Size: 609 78 1331 182 164 927 776 677
Cuts at: 6350 7498 7743 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1331 1148 927 776 677 609 245
182 164 78
NaeI GCC'GGC
Cuts at: 3592 3592
Size: 8257
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7705
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8257
NgoMIV G'CCGG_C
Cuts at: 3590 3590
Size: 8257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8257
NotI GC'GGCC_GC
Cuts at: 5704 5704
Size: 8257
NspI r_CATG'y
Cuts at: 1132 1229 2585 2866 3238 4147 5386 5615 7160
Size: 97 1356 281 372 909 1239 229 1545
Cuts at: 7160 1132
Size: 2229
Fragments arranged by size:
2229 1545 1356 1239 909 372 281 229
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8257
PciI A'CATG_T
Cuts at: 3234 7156 3234
Size: 3922 4335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2161 2380 3193 3769 1108
Size: 1053 219 813 576 5596
Fragments arranged by size:
5596 1053 813 576 219
PfoI T'CCnGG_A
Cuts at: 3501 3501
Size: 8257
PmeI GTTT'AAAC
Cuts at: 5561 5561
Size: 8257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 6326 6358 7839 7871 2828
Size: 32 3466 32 1481 32 3214
Fragments arranged by size:
3466 3214 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2980 2987 4429 5074 5259 5436 5491 2980
Size: 7 1442 645 185 177 55 5746
Fragments arranged by size:
5746 1442 645 185 177 55 7
PshAI GACnn'nnGTC
Cuts at: 1613 2985 1613
Size: 1372 6885
PsiI TTA'TAA
Cuts at: 5827 5827
Size: 8257
PspOMI G'GGCC_C
Cuts at: 2563 3799 4784 2563
Size: 1236 985 6036
Fragments arranged by size:
6036 1236 985
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 8225
PstI C_TGCA'G
Cuts at: 830 4443 5242 5609 830
Size: 3613 799 367 3478
Fragments arranged by size:
3613 3478 799 367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8257
PvuII CAG'CTG
Cuts at: 2215 2293 3970 4897 2215
Size: 78 1677 927 5575
Fragments arranged by size:
5575 1677 927 78
SacI G_AGCT'C
Cuts at: 2482 4860 5348 2482
Size: 2378 488 5391
Fragments arranged by size:
5391 2378 488
SalI G'TCGA_C
Cuts at: 1936 2925 5599 1936
Size: 989 2674 4594
Fragments arranged by size:
4594 2674 989
SanDI GG'GwC_CC
Cuts at: 2987 4429 5491 2987
Size: 1442 1062 5753
Fragments arranged by size:
5753 1442 1062
SapI GCTCTTCn'nnn_
Cuts at: 3108 5097 3108
Size: 1989 6268
SbfI CC_TGCA'GG
Cuts at: 4443 5609 4443
Size: 1166 7091
ScaI AGT'ACT
Cuts at: 4958 6505 8217 4958
Size: 1547 1712 4998
Fragments arranged by size:
4998 1712 1547
SexAI A'CCwGG_T
Cuts at: 2779 2779
Size: 8257
SfcI C'TryA_G
Cuts at: 826 1045 3910 4439 5238 5605 6740 7421 7612
Size: 219 2865 529 799 367 1135 681 191
Cuts at: 7612 826
Size: 1471
Fragments arranged by size:
2865 1471 1135 799 681 529 367 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8257
SgrAI Cr'CCGG_yG
Cuts at: 1700 4507 1700
Size: 2807 5450
SmaI CCC'GGG
Cuts at: 1872 4583 5584 1872
Size: 2711 1001 4545
Fragments arranged by size:
4545 2711 1001
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4138 6361 7262 7524 820
Size: 197 926 2195 2223 901 262 1553
Fragments arranged by size:
2223 2195 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6048 493
Size: 5555 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8257
SphI G_CATG'C
Cuts at: 1132 5386 5615 1132
Size: 4254 229 3774
Fragments arranged by size:
4254 3774 229
SspI AAT'ATT
Cuts at: 5 52 6181 5
Size: 47 6129 2081
Fragments arranged by size:
6129 2081 47
StuI AGG'CCT
Cuts at: 1517 4639 1517
Size: 3122 5135
StyI C'CwwG_G
Cuts at: 513 1065 2547 2821 3108 3307 5696 513
Size: 552 1482 274 287 199 2389 3074
Fragments arranged by size:
3074 2389 1482 552 287 274 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5413 6284 6301 6454 6639 7084 325
Size: 1255 3833 871 17 153 185 445 1498
Fragments arranged by size:
3833 1498 1255 871 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4174 4470 4956
Size: 274 80 33 51 1443 2197 296 486
Cuts at: 4956 6503 8215 96
Size: 1547 1712 138
Fragments arranged by size:
2197 1712 1547 1443 486 296 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2512 2544 2745 2777 8217 8249 2512
Size: 32 201 32 5440 32 2520
Fragments arranged by size:
5440 2520 201 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4538 1316
Size: 3222 5035
XbaI T'CTAG_A
Cuts at: 2932 5593 2932
Size: 2661 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2828 2836 3253 4669 5134 5386 2347
Size: 481 8 417 1416 465 252 5218
Fragments arranged by size:
5218 1416 481 465 417 252 8
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8257
XmaI C'CCGG_G
Cuts at: 1870 4581 5582 1870
Size: 2711 1001 4545
Fragments arranged by size:
4545 2711 1001
XmnI GAAnn'nnTTC
Cuts at: 3102 6386 3102
Size: 3284 4973
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6065 276
Size: 53 83 186 936 4531 2468
Fragments arranged by size:
4531 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI RsrII SacII SfiI SfoI SrfI
SwaI