Restriction map for
pFN21AB3020
(Circular) (Six-base) MAPSORT of: pFN21AB3020.seq Check: 2172 from: 1 to: 6628
pFN21AB3020 6628 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3055 3295 3055
Size: 240 6388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3971 1937
Size: 2034 4594
Acc65I G'GTAC_C
Cuts at: 2497 3949 2497
Size: 1452 5176
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3042 3482 3608 3939 4691 6075 6343 1632
Size: 1410 440 126 331 752 1384 268 1917
Fragments arranged by size:
1917 1410 1384 752 440 331 268 126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 3822 5527 6504 1203
Size: 2619 1705 977 1327
Fragments arranged by size:
2619 1705 1327 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 3196 5357 3196
Size: 2161 4467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2096 2402 1184
Size: 912 306 5410
Fragments arranged by size:
5410 912 306
AlwNI CAG_nnn'CTG
Cuts at: 2494 3501 4371 5943 2494
Size: 1007 870 1572 3179
Fragments arranged by size:
3179 1572 1007 870
ApaI G_GGCC'C
Cuts at: 3483 3770 3788 3483
Size: 287 18 6323
Fragments arranged by size:
6323 287 18
ApaLI G'TGCA_C
Cuts at: 3308 4685 5841 3308
Size: 1377 1156 4095
Fragments arranged by size:
4095 1377 1156
ApoI r'AATT_y
Cuts at: 1493 3937 4163 4315 6424 1493
Size: 2444 226 152 2109 1697
Fragments arranged by size:
2444 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 3363 3761 3833 3953 1870
Size: 73 700 720 398 72 120 4545
Fragments arranged by size:
4545 720 700 398 120 73 72
BamHI G'GATC_C
Cuts at: 3535 3958 4325 3535
Size: 423 367 5838
Fragments arranged by size:
5838 423 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2497 2873 3406 3949 5404
Size: 325 217 627 710 376 533 543 1455
Cuts at: 5404 618
Size: 1842
Fragments arranged by size:
1842 1455 710 627 543 533 376 325
217
BanII G_rGCy'C
Cuts at: 1391 3483 3551 3760 3770 3788 3947 1391
Size: 2092 68 209 10 18 159 4072
Fragments arranged by size:
4072 2092 209 159 68 18 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3030 3405 3596 928
Size: 971 1131 375 191 3960
Fragments arranged by size:
3960 1131 971 375 191
BbvCI CC'TCA_GC
Cuts at: 3813 3813
Size: 6628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2590 2624 4819 4853 1903
Size: 34 653 34 2195 34 3678
Fragments arranged by size:
3678 2195 653 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4520 5730 4520
Size: 1210 5418
BclI T'GATC_A
Cuts at: 1548 3280 1548
Size: 1732 4896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2180 3913
Size: 107 122 71 631 605 368 140 1733
Cuts at: 3913 5239 136
Size: 1326 1525
Fragments arranged by size:
1733 1525 1326 631 605 368 140 122
107 71
BglII A'GATC_T
Cuts at: 2454 3078 3090 6623 2454
Size: 624 12 3533 2459
Fragments arranged by size:
3533 2459 624 12
BlpI GC'TnA_GC
Cuts at: 4045 4045
Size: 6628
Bme1580I G_kGCm'C
Cuts at: 1790 2779 3312 3411 3483 3770 3788 4689 5845
Size: 989 533 99 72 287 18 901 1156
Cuts at: 5845 1790
Size: 2573
Fragments arranged by size:
2573 1156 989 901 533 287 99 72
18
BmgBI CAC'GTC
Cuts at: 1318 2131 1318
Size: 813 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 2754 4359
Size: 416 53 83 399 24 798 539 1605
Cuts at: 4359 5317 442
Size: 958 1753
Fragments arranged by size:
1753 1605 958 798 539 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2945 3256 5288 1239
Size: 1097 609 311 2032 2579
Fragments arranged by size:
2579 2032 1097 609 311
Bpu10I CC'TnA_GC
Cuts at: 3813 3813
Size: 6628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4753 5618 5916 4753
Size: 865 298 5465
Fragments arranged by size:
5465 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3360 5291 882
Size: 617 1861 1931 2219
Fragments arranged by size:
2219 1931 1861 617
BsaAI yAC'GTr
Cuts at: 493 1204 4419 493
Size: 711 3215 2702
Fragments arranged by size:
3215 2702 711
BsaBI GATnn'nnATC
Cuts at: 3105 6622 3105
Size: 3517 3111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3593 4435 4817 5452
Size: 53 83 186 936 2060 842 382 635
Cuts at: 5452 275
Size: 1451
Fragments arranged by size:
2060 1451 936 842 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3662 3692 1178
Size: 30 165 30 2259 30 4114
Fragments arranged by size:
4114 2259 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2645 2648 2976 3061 3801 3821 1186
Size: 1459 3 328 85 740 20 3993
Fragments arranged by size:
3993 1459 740 328 85 20 3
BseYI C'CCAG_C
Cuts at: 2161 2940 2965 2995 3482 5831 6327 2161
Size: 779 25 30 487 2349 496 2462
Fragments arranged by size:
2462 2349 779 496 487 30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2676 1163
Size: 573 333 607 5115
Fragments arranged by size:
5115 607 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2421 4078 4839 5867 1540
Size: 275 186 420 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 420 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 3312 3551 4689 4774 5845 2136
Size: 1176 239 1138 85 1071 2919
Fragments arranged by size:
2919 1176 1138 1071 239 85
BsmI GAATG_Cn'
Cuts at: 4139 4232 4139
Size: 93 6535
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2779 3312 3411 3483 3551 3760
Size: 399 346 643 533 99 72 68 209
Cuts at: 3760 3770 3788 3947 4689 4774 5845 1391
Size: 10 18 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 533 399 346 209
159 99 85 72 68 18 10
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 2412 3394 4517 6247 2412
Size: 982 1123 1730 2793
Fragments arranged by size:
2793 1730 1123 982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2835 3055 3295 3350 3983 844
Size: 1991 220 240 55 633 3489
Fragments arranged by size:
3489 1991 633 240 220 55
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 3322 3711 4515 1394
Size: 429 188 1311 389 804 3507
Fragments arranged by size:
3507 1311 804 429 389 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 2661 3352 5123 5297 65
Size: 1183 1360 53 691 1771 174 1396
Fragments arranged by size:
1771 1396 1360 1183 691 174 53
BsrFI r'CCGG_y
Cuts at: 1700 1810 2612 2919 3117 5272 5459 1700
Size: 110 802 307 198 2155 187 2869
Fragments arranged by size:
2869 2155 802 307 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 2933 4688 5700 2933
Size: 1755 1012 3861
Fragments arranged by size:
3861 1755 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2663 2774 3492 794
Size: 1869 111 718 3930
Fragments arranged by size:
3930 1869 718 111
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 3563 1184
Size: 2379 4249
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 3257 3422 2830
Size: 427 165 6036
Fragments arranged by size:
6036 427 165
BstYI r'GATC_y
Cuts at: 1972 2454 2490 3078 3090 3535 3886 3958 4325
Size: 482 36 588 12 445 351 72 367
Cuts at: 4325 4710 4727 6179 6265 6277 6623 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 588 482 445 385 367 351
346 86 72 36 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2861 3891 2861
Size: 1030 5598
BtgI C'CryG_G
Cuts at: 513 1065 2004 2712 3228 3909 513
Size: 552 939 708 516 681 3232
Fragments arranged by size:
3232 939 708 681 552 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2593 487
Size: 683 56 1367 4522
Fragments arranged by size:
4522 1367 683 56
BtsI GCAGTG_nn'
Cuts at: 4144 4938 4958 4144
Size: 794 20 5814
Fragments arranged by size:
5814 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6593
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3638 5635 809
Size: 507 2322 1997 1802
Fragments arranged by size:
2322 1997 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2418 4075
Size: 54 1425 50 275 18 351 237 1657
Cuts at: 4075 4964 6593 8
Size: 889 1629 43
Fragments arranged by size:
1657 1629 1425 889 351 275 237 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2418 4075 1537
Size: 275 606 1657 4090
Fragments arranged by size:
4090 1657 606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4558 1353
Size: 3205 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2250 5215 5589 5735 387
Size: 1064 138 661 2965 374 146 1280
Fragments arranged by size:
2965 1280 1064 661 374 146 138
EcoICRI GAG'CTC
Cuts at: 3549 3549
Size: 6628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2945 3042 3256 3482 3608 3939
Size: 393 704 609 97 214 226 126 331
Cuts at: 3939 4691 5288 6075 6343 1239
Size: 752 597 787 268 1524
Fragments arranged by size:
1524 787 752 704 609 597 393 331
268 226 214 126 97
EcoNI CCTnn'n_nnAGG
Cuts at: 2864 2864
Size: 6628
EcoO109I rG'GnC_Cy
Cuts at: 2541 2847 2857 3479 3766 3767 3778 3785 3914
Size: 306 10 622 287 1 11 7 129
Cuts at: 3914 2541
Size: 5255
Fragments arranged by size:
5255 622 306 287 129 11 10 7
1
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3739 3739
Size: 6628
FspI TGC'GCA
Cuts at: 5134 5134
Size: 6628
HaeII r_GCGC'y
Cuts at: 1678 2122 2220 5775 1678
Size: 444 98 3555 2531
Fragments arranged by size:
3555 2531 444 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3182 3214 5269 5301 5343
Size: 32 135 32 1610 32 2055 32 42
Cuts at: 5343 5375 1373
Size: 32 2626
Fragments arranged by size:
2626 2055 1610 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3898 3972 4218 669
Size: 988 281 1960 74 246 3079
Fragments arranged by size:
3079 1960 988 281 246 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6628
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KpnI G_GTAC'C
Cuts at: 2501 3953 2501
Size: 1452 5176
MfeI C'AATT_G
Cuts at: 4227 4227
Size: 6628
MluI A'CGCG_T
Cuts at: 6504 6504
Size: 6628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2505 5742 5926 1305
Size: 1200 3237 184 2007
Fragments arranged by size:
3237 2007 1200 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4806
Fragments arranged by size:
4806 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2384 4586 4945 5104 518
Size: 648 912 306 2202 359 159 2042
Fragments arranged by size:
2202 2042 912 648 359 306 159
MspA1I CmG'CkG
Cuts at: 1606 2965 4044 4721 5869 6114 1606
Size: 1359 1079 677 1148 245 2120
Fragments arranged by size:
2120 1359 1148 1079 677 245
NaeI GCC'GGC
Cuts at: 2614 2614
Size: 6628
NcoI C'CATG_G
Cuts at: 513 1065 2004 2712 3909 513
Size: 552 939 708 1197 3232
Fragments arranged by size:
3232 1197 939 708 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6628
NgoMIV G'CCGG_C
Cuts at: 2612 2612
Size: 6628
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 2623 3199 3752 3826 3986 5531 1132
Size: 97 1394 576 553 74 160 1545 2229
Fragments arranged by size:
2229 1545 1394 576 553 160 97 74
PasI CC'CwG_GG
Cuts at: 1348 2537 2852 3772 1348
Size: 1189 315 920 4204
Fragments arranged by size:
4204 1189 920 315
PciI A'CATG_T
Cuts at: 3822 5527 3822
Size: 1705 4923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6628
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2195 2227 4697 4729 6210 6242 2195
Size: 32 2470 32 1481 32 2581
Fragments arranged by size:
2581 2470 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2541 2847 3778 2541
Size: 306 931 5391
Fragments arranged by size:
5391 931 306
PshAI GACnn'nnGTC
Cuts at: 1613 2250 1613
Size: 637 5991
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PspOMI G'GGCC_C
Cuts at: 3479 3766 3784 3479
Size: 287 18 6323
Fragments arranged by size:
6323 287 18
PspXI vC'TCGA_Gb
Cuts at: 3833 3833
Size: 6628
PstI C_TGCA'G
Cuts at: 830 2192 3497 3980 830
Size: 1362 1305 483 3478
Fragments arranged by size:
3478 1362 1305 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
PvuII CAG'CTG
Cuts at: 2965 2965
Size: 6628
SacI G_AGCT'C
Cuts at: 3551 3551
Size: 6628
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SanDI GG'GwC_CC
Cuts at: 2847 2847
Size: 6628
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 2198 3431 4876 6588 2198
Size: 1233 1445 1712 2238
Fragments arranged by size:
2238 1712 1445 1233
SexAI A'CCwGG_T
Cuts at: 2055 2055
Size: 6628
SfcI C'TryA_G
Cuts at: 826 1045 2188 3493 3976 5111 5792 5983 826
Size: 219 1143 1305 483 1135 681 191 1471
Fragments arranged by size:
1471 1305 1143 1135 681 483 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 3955 1872
Size: 2083 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3833 4732 5633 5895 820
Size: 197 926 1890 899 901 262 1553
Fragments arranged by size:
1890 1553 926 901 899 262 197
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 2623 3986 1132
Size: 1491 1363 3774
Fragments arranged by size:
3774 1491 1363
SspI AAT'ATT
Cuts at: 5 52 4552 5
Size: 47 4500 2081
Fragments arranged by size:
4500 2081 47
StuI AGG'CCT
Cuts at: 1517 3277 1517
Size: 1760 4868
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2586 2712 2813 3793 3909
Size: 552 939 333 249 126 101 980 116
Cuts at: 3909 4067 513
Size: 158 3074
Fragments arranged by size:
3074 980 939 552 333 249 158 126
116 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2987 4655 4672 4825 5010 5455 325
Size: 1255 1407 1668 17 153 185 445 1498
Fragments arranged by size:
1668 1498 1407 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3429 4874
Size: 274 80 33 51 1443 219 1233 1445
Cuts at: 4874 6586 96
Size: 1712 138
Fragments arranged by size:
1712 1445 1443 1233 274 219 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 6588 6620 3113
Size: 32 3443 32 3121
Fragments arranged by size:
3443 3121 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6628
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2962 3064 3652 2962
Size: 102 588 5938
Fragments arranged by size:
5938 588 102
XhoI C'TCGA_G
Cuts at: 1943 3833 1943
Size: 1890 4738
XmaI C'CCGG_G
Cuts at: 1870 3953 1870
Size: 2083 4545
XmnI GAAnn'nnTTC
Cuts at: 3266 4757 3266
Size: 1491 5137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI