Restriction map for
pFN21AB3015
(Circular) (Six-base) MAPSORT of: pFN21AB3015.seq Check: 2697 from: 1 to: 6913
pFN21AB3015 6913 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2815 3265 2815
Size: 450 6463
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3072 4723 278
Size: 53 83 186 936 1536 1651 2468
Fragments arranged by size:
2468 1651 1536 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3748 4256 1937
Size: 1811 508 4594
Fragments arranged by size:
4594 1811 508
Acc65I G'GTAC_C
Cuts at: 4234 4234
Size: 6913
AclI AA'CG_TT
Cuts at: 1563 5040 5413 1563
Size: 3477 373 3063
Fragments arranged by size:
3477 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2889 3432 4976 6360 6628 1632
Size: 1257 543 1544 1384 268 1917
Fragments arranged by size:
1917 1544 1384 1257 543 268
AfeI AGC'GCT
Cuts at: 3722 3722
Size: 6913
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6716
AflIII A'CryG_T
Cuts at: 1203 5812 6789 1203
Size: 4609 977 1327
Fragments arranged by size:
4609 1327 977
AgeI A'CCGG_T
Cuts at: 2148 5744 2148
Size: 3596 3317
AhdI GACnn_n'nnGTC
Cuts at: 5642 5642
Size: 6913
AleI CACnn'nnGTG
Cuts at: 1166 3035 1166
Size: 1869 5044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6913
AlwNI CAG_nnn'CTG
Cuts at: 2380 4656 6228 2380
Size: 2276 1572 3065
Fragments arranged by size:
3065 2276 1572
ApaLI G'TGCA_C
Cuts at: 4970 6126 4970
Size: 1156 5757
ApoI r'AATT_y
Cuts at: 1493 2384 2519 2690 3010 4222 4448 4600 6709
Size: 891 135 171 320 1212 226 152 2109
Cuts at: 6709 1493
Size: 1697
Fragments arranged by size:
2109 1697 1212 891 320 226 171 152
135
AseI AT'TA_AT
Cuts at: 160 1032 5467 160
Size: 872 4435 1606
Fragments arranged by size:
4435 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
AvaI C'yCGr_G
Cuts at: 1870 1943 4238 1870
Size: 73 2295 4545
Fragments arranged by size:
4545 2295 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3054 3087 3054
Size: 33 6880
BamHI G'GATC_C
Cuts at: 4243 4610 4243
Size: 367 6546
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3125 4234 5689 618
Size: 325 217 627 1338 1109 1455 1842
Fragments arranged by size:
1842 1455 1338 1109 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3341 4232 1391
Size: 1950 891 4072
Fragments arranged by size:
4072 1950 891
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5942
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5104 5138 1903
Size: 34 3167 34 3678
Fragments arranged by size:
3678 3167 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4805 6015 3686
Size: 1119 1210 4584
Fragments arranged by size:
4584 1210 1119
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6913
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5524 136
Size: 107 122 71 631 605 3852 1525
Fragments arranged by size:
3852 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3285 6908 3285
Size: 3623 3290
BlpI GC'TnA_GC
Cuts at: 4330 4330
Size: 6913
Bme1580I G_kGCm'C
Cuts at: 1790 2903 3128 4974 6130 1790
Size: 1113 225 1846 1156 2573
Fragments arranged by size:
2573 1846 1156 1113 225
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6913
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2736 4644 5602
Size: 416 53 83 399 24 1319 1908 958
Cuts at: 5602 442
Size: 1753
Fragments arranged by size:
1908 1753 1319 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3229 3642 4017 5573 1239
Size: 1990 413 375 1556 2579
Fragments arranged by size:
2579 1990 1556 413 375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 5038 5903 6201 2170
Size: 2868 865 298 2882
Fragments arranged by size:
2882 2868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5576 882
Size: 617 4077 2219
Fragments arranged by size:
4077 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4704 493
Size: 711 3500 2702
Fragments arranged by size:
3500 2702 711
BsaBI GATnn'nnATC
Cuts at: 6907 6907
Size: 6913
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3069 4720 5102 5737
Size: 53 83 186 936 1536 1651 382 635
Cuts at: 5737 275
Size: 1451
Fragments arranged by size:
1651 1536 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2148 5346 5744 6018 6165 6576 2148
Size: 3198 398 274 147 411 2485
Fragments arranged by size:
3198 2485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3217 3247 3785 3815 1178
Size: 30 165 30 1814 30 538 30 4276
Fragments arranged by size:
4276 1814 538 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2538 2586 2814 1186
Size: 1352 48 228 5285
Fragments arranged by size:
5285 1352 228 48
BseYI C'CCAG_C
Cuts at: 3465 4098 4130 6116 6612 3465
Size: 633 32 1986 496 3766
Fragments arranged by size:
3766 1986 633 496 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3699 1163
Size: 573 1963 4377
Fragments arranged by size:
4377 1963 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4363 5124 6152 1540
Size: 275 186 2362 761 1028 2301
Fragments arranged by size:
2362 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4974 5059 6130 4974
Size: 85 1071 5757
Fragments arranged by size:
5757 1071 85
BsmI GAATG_Cn'
Cuts at: 2426 2795 4424 4517 2426
Size: 369 1629 93 4822
Fragments arranged by size:
4822 1629 369 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3501 3512 3501
Size: 11 6902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2903 3128 3341 4232 4974 5059 6130
Size: 399 1113 225 213 891 742 85 1071
Cuts at: 6130 1391
Size: 2174
Fragments arranged by size:
2174 1113 1071 891 742 399 225 213
85
BspEI T'CCGG_A
Cuts at: 6576 6576
Size: 6913
BspHI T'CATG_A
Cuts at: 4802 6532 4802
Size: 1730 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2556 2815 3265 4268 844
Size: 1712 259 450 1003 3489
Fragments arranged by size:
3489 1712 1003 450 259
BsrBI CCG'CTC
Cuts at: 1394 1823 4800 1394
Size: 429 2977 3507
Fragments arranged by size:
3507 2977 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2865 5408 5582 65
Size: 1183 1617 2543 174 1396
Fragments arranged by size:
2543 1617 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 4026 5557 5744 1700
Size: 110 338 1878 1531 187 2869
Fragments arranged by size:
2869 1878 1531 338 187 110
BsrGI T'GTAC_A
Cuts at: 96 2347 96
Size: 2251 4662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6913
BssSI C'ACGA_G
Cuts at: 4973 5985 4973
Size: 1012 5901
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6913
BstBI TT'CG_AA
Cuts at: 3688 5717 3688
Size: 2029 4884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6913
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2207 2122
Size: 85 6828
BstYI r'GATC_y
Cuts at: 1972 2494 2884 3285 4164 4243 4610 4995 5012
Size: 522 390 401 879 79 367 385 17
Cuts at: 5012 6464 6550 6562 6908 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 879 522 401 390 385 367
346 86 79 17 12
BstZ17I GTA'TAC
Cuts at: 3749 3749
Size: 6913
BtgI C'CryG_G
Cuts at: 513 1065 3311 513
Size: 552 2246 4115
Fragments arranged by size:
4115 2246 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 5279
Fragments arranged by size:
5279 895 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 2403 4044 4429 5223 5243 2280
Size: 123 1641 385 794 20 3950
Fragments arranged by size:
3950 1641 794 385 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6878
DraI TTT'AAA
Cuts at: 4217 4217
Size: 6913
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2626 5920 809
Size: 507 1310 3294 1802
Fragments arranged by size:
3294 1802 1310 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3264 4360 5249
Size: 54 1425 50 275 18 1434 1096 889
Cuts at: 5249 6878 8
Size: 1629 43
Fragments arranged by size:
1629 1434 1425 1096 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4360 1537
Size: 275 2548 4090
Fragments arranged by size:
4090 2548 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 2232 4843 1353
Size: 876 3 2611 3423
Fragments arranged by size:
3423 2611 876 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5500 5874 6020 387
Size: 1064 138 3911 374 146 1280
Fragments arranged by size:
3911 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2889 3229 3432 3642 4017 4976 5573
Size: 393 1257 340 203 210 375 959 597
Cuts at: 5573 6360 6628 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1257 959 787 597 393 375 340
268 210 203
EcoNI CCTnn'n_nnAGG
Cuts at: 3057 3057
Size: 6913
EcoRI G'AATT_C
Cuts at: 4222 4600 4222
Size: 378 6535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3814 3814
Size: 6913
FspI TGC'GCA
Cuts at: 5419 5419
Size: 6913
HaeII r_GCGC'y
Cuts at: 1678 3724 6060 1678
Size: 2046 2336 2531
Fragments arranged by size:
2531 2336 2046
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3527 3559 3614 3646 5554
Size: 32 135 32 1955 32 55 32 1908
Cuts at: 5554 5586 5628 5660 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1955 1908 135 55 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3382 3828 4257 4503 669
Size: 988 281 1104 340 446 429 246 3079
Fragments arranged by size:
3079 1104 988 446 429 340 281 246
HindIII A'AGCT_T
Cuts at: 748 3650 748
Size: 2902 4011
HpaI GTT'AAC
Cuts at: 3042 4503 3042
Size: 1461 5452
KpnI G_GTAC'C
Cuts at: 4238 4238
Size: 6913
MfeI C'AATT_G
Cuts at: 4512 4512
Size: 6913
MluI A'CGCG_T
Cuts at: 6789 6789
Size: 6913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3141 6027 6211 1305
Size: 1836 2886 184 2007
Fragments arranged by size:
2886 2007 1836 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3266 10
Size: 54 1425 343 1434 3657
Fragments arranged by size:
3657 1434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2988 3035 4871 5230 5389 518
Size: 648 954 868 47 1836 359 159 2042
Fragments arranged by size:
2042 1836 954 868 648 359 159 47
MspA1I CmG'CkG
Cuts at: 1606 2086 2134 3667 3932 4329 5006 6154 6399
Size: 480 48 1533 265 397 677 1148 245
Cuts at: 6399 1606
Size: 2120
Fragments arranged by size:
2120 1533 1148 677 480 397 265 245
48
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6361
NdeI CA'TA_TG
Cuts at: 387 2623 2989 3484 387
Size: 2236 366 495 3816
Fragments arranged by size:
3816 2236 495 366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6913
NotI GC'GGCC_GC
Cuts at: 4360 4360
Size: 6913
NspI r_CATG'y
Cuts at: 1132 1229 2169 4271 5816 1132
Size: 97 940 2102 1545 2229
Fragments arranged by size:
2229 2102 1545 940 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6913
PciI A'CATG_T
Cuts at: 5812 5812
Size: 6913
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6913
PfoI T'CCnGG_A
Cuts at: 2805 2805
Size: 6913
PmeI GTTT'AAAC
Cuts at: 4217 4217
Size: 6913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 6495 6527 4982
Size: 32 1481 32 5368
Fragments arranged by size:
5368 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6913
PsiI TTA'TAA
Cuts at: 3395 4483 3395
Size: 1088 5825
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3984 4016 3984
Size: 32 6881
PstI C_TGCA'G
Cuts at: 830 2410 3937 4265 830
Size: 1580 1527 328 3478
Fragments arranged by size:
3478 1580 1527 328
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6913
PvuII CAG'CTG
Cuts at: 3667 3932 3667
Size: 265 6648
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 6913
SalI G'TCGA_C
Cuts at: 1936 4255 1936
Size: 2319 4594
SbfI CC_TGCA'GG
Cuts at: 4265 4265
Size: 6913
ScaI AGT'ACT
Cuts at: 3400 3409 3926 3979 5161 6873 3400
Size: 9 517 53 1182 1712 3440
Fragments arranged by size:
3440 1712 1182 517 53 9
SexAI A'CCwGG_T
Cuts at: 3346 3346
Size: 6913
SfcI C'TryA_G
Cuts at: 826 1045 2406 3933 4261 5396 6077 6268 826
Size: 219 1361 1527 328 1135 681 191 1471
Fragments arranged by size:
1527 1471 1361 1135 681 328 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6913
SmaI CCC'GGG
Cuts at: 1872 4240 1872
Size: 2368 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2185 5017 5918 6180 820
Size: 197 926 242 2832 901 262 1553
Fragments arranged by size:
2832 1553 926 901 262 242 197
SnaBI TAC'GTA
Cuts at: 493 4704 493
Size: 4211 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6913
SphI G_CATG'C
Cuts at: 1132 2169 4271 1132
Size: 1037 2102 3774
Fragments arranged by size:
3774 2102 1037
SspI AAT'ATT
Cuts at: 5 52 2356 4837 5
Size: 47 2304 2481 2081
Fragments arranged by size:
2481 2304 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6913
StyI C'CwwG_G
Cuts at: 513 1065 4352 513
Size: 552 3287 3074
Fragments arranged by size:
3287 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4940 4957 5110 5295 5740 325
Size: 1255 3360 17 153 185 445 1498
Fragments arranged by size:
3360 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2347 3398 3407
Size: 274 80 33 51 1443 370 1051 9
Cuts at: 3407 3924 3977 4199 5159 6871 96
Size: 517 53 222 960 1712 138
Fragments arranged by size:
1712 1443 1051 960 517 370 274 222
138 80 53 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 2927 2959 3217 3249 6873 6905 2306
Size: 32 589 32 258 32 3624 32 2314
Fragments arranged by size:
3624 2314 589 258 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 1316
Size: 1401 5512
XbaI T'CTAG_A
Cuts at: 2497 4249 2497
Size: 1752 5161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2702 2702
Size: 6913
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6913
XmaI C'CCGG_G
Cuts at: 1870 4238 1870
Size: 2368 4545
XmnI GAAnn'nnTTC
Cuts at: 5042 5042
Size: 6913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3070 4721 276
Size: 53 83 186 936 1536 1651 2468
Fragments arranged by size:
2468 1651 1536 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI