Restriction map for
pFN21AB3014
(Circular) (Six-base) MAPSORT of: pFN21AB3014.seq Check: 6807 from: 1 to: 7735
pFN21AB3014 7735 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5545 278
Size: 53 83 186 936 4009 2468
Fragments arranged by size:
4009 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5078 1937
Size: 3141 4594
Acc65I G'GTAC_C
Cuts at: 5056 5056
Size: 7735
AclI AA'CG_TT
Cuts at: 1563 5862 6235 1563
Size: 4299 373 3063
Fragments arranged by size:
4299 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2415 2753 3669 4335 4866 4952 5798 7182
Size: 783 338 916 666 531 86 846 1384
Cuts at: 7182 7450 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 916 846 783 666 531 338
268 86
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7538
AflIII A'CryG_T
Cuts at: 1203 3732 4024 4327 6634 7611 1203
Size: 2529 292 303 2307 977 1327
Fragments arranged by size:
2529 2307 1327 977 303 292
AgeI A'CCGG_T
Cuts at: 6566 6566
Size: 7735
AhdI GACnn_n'nnGTC
Cuts at: 6464 6464
Size: 7735
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2993 1184
Size: 1809 5926
AlwNI CAG_nnn'CTG
Cuts at: 2120 3119 3932 4252 4547 4820 5478 7050 2120
Size: 999 813 320 295 273 658 1572 2805
Fragments arranged by size:
2805 1572 999 813 658 320 295 273
ApaLI G'TGCA_C
Cuts at: 4825 5792 6948 4825
Size: 967 1156 5612
Fragments arranged by size:
5612 1156 967
ApoI r'AATT_y
Cuts at: 1493 2207 3363 4622 4695 5044 5270 5422 7531
Size: 714 1156 1259 73 349 226 152 2109
Cuts at: 7531 1493
Size: 1697
Fragments arranged by size:
2109 1697 1259 1156 714 349 226 152
73
AseI AT'TA_AT
Cuts at: 160 1032 4593 6289 160
Size: 872 3561 1696 1606
Fragments arranged by size:
3561 1696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7735
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 4488 5060 1870
Size: 73 70 2475 572 4545
Fragments arranged by size:
4545 2475 572 73 70
BamHI G'GATC_C
Cuts at: 4128 5065 5432 4128
Size: 937 367 6431
Fragments arranged by size:
6431 937 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3776 5056 6511 618
Size: 325 217 627 1989 1280 1455 1842
Fragments arranged by size:
1989 1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3597 5054 1391
Size: 2206 1457 4072
Fragments arranged by size:
4072 2206 1457
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3851 4132 928
Size: 971 1952 281 4531
Fragments arranged by size:
4531 1952 971 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5926 5960 1903
Size: 34 3989 34 3678
Fragments arranged by size:
3989 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3747 5627 6837 3747
Size: 1880 1210 4645
Fragments arranged by size:
4645 1880 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7735
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 2986 4433
Size: 107 122 71 631 605 874 440 1447
Cuts at: 4433 6346 136
Size: 1913 1525
Fragments arranged by size:
1913 1525 1447 874 631 605 440 122
107 71
BglII A'GATC_T
Cuts at: 5013 7730 5013
Size: 2717 5018
BlpI GC'TnA_GC
Cuts at: 5152 5152
Size: 7735
Bme1580I G_kGCm'C
Cuts at: 1790 4829 5796 6952 1790
Size: 3039 967 1156 2573
Fragments arranged by size:
3039 2573 1156 967
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7735
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4830 5466 6424
Size: 416 53 83 399 24 3413 636 958
Cuts at: 6424 442
Size: 1753
Fragments arranged by size:
3413 1753 958 636 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2574 2690 3060 3119 3275 3345 3765 3896
Size: 1335 116 370 59 156 70 420 131
Cuts at: 3896 3909 4144 6395 1239
Size: 13 235 2251 2579
Fragments arranged by size:
2579 2251 1335 420 370 235 156 131
116 70 59 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3069 3224 5860 6725 7023 3069
Size: 155 2636 865 298 3781
Fragments arranged by size:
3781 2636 865 298 155
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6398 882
Size: 617 4899 2219
Fragments arranged by size:
4899 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4328 5526 493
Size: 711 3124 1198 2702
Fragments arranged by size:
3124 2702 1198 711
BsaBI GATnn'nnATC
Cuts at: 7729 7729
Size: 7735
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5542 5924 6559 275
Size: 53 83 186 936 4009 382 635 1451
Fragments arranged by size:
4009 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4805 6168 6566 6840 6987 7398 4805
Size: 1363 398 274 147 411 5142
Fragments arranged by size:
5142 1363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2580 2610 4800 4830 1178
Size: 30 165 30 1177 30 2190 30 4083
Fragments arranged by size:
4083 2190 1177 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3910 1186
Size: 2724 5011
BseYI C'CCAG_C
Cuts at: 2243 2644 3513 3761 4547 6938 7434 2243
Size: 401 869 248 786 2391 496 2544
Fragments arranged by size:
2544 2391 869 786 496 401 248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 2953 4491 1163
Size: 573 706 511 1538 4407
Fragments arranged by size:
4407 1538 706 573 511
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5185 5946 6974 1540
Size: 275 186 3184 761 1028 2301
Fragments arranged by size:
3184 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3700 4829 5796 5881 6952 3700
Size: 1129 967 85 1071 4483
Fragments arranged by size:
4483 1129 1071 967 85
BsmI GAATG_Cn'
Cuts at: 5246 5339 5246
Size: 93 7642
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3597 3700 4829 5054 5796 5881 6952
Size: 399 1807 103 1129 225 742 85 1071
Cuts at: 6952 1391
Size: 2174
Fragments arranged by size:
2174 1807 1129 1071 742 399 225 103
85
BspEI T'CCGG_A
Cuts at: 7398 7398
Size: 7735
BspHI T'CATG_A
Cuts at: 4689 5624 7354 4689
Size: 935 1730 5070
Fragments arranged by size:
5070 1730 935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4473 5090 844
Size: 3629 617 3489
Fragments arranged by size:
3629 3489 617
BsrBI CCG'CTC
Cuts at: 1394 1823 5622 1394
Size: 429 3799 3507
Fragments arranged by size:
3799 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2285 3564 3874 6230 6404 65
Size: 1183 1037 1279 310 2356 174 1396
Fragments arranged by size:
2356 1396 1279 1183 1037 310 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6379 6566 1700
Size: 110 4569 187 2869
Fragments arranged by size:
4569 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2515 4831 4990 96
Size: 2419 2316 159 2841
Fragments arranged by size:
2841 2419 2316 159
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7735
BssSI C'ACGA_G
Cuts at: 4308 5795 6807 4308
Size: 1487 1012 5236
Fragments arranged by size:
5236 1487 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2120 2124 3119 794
Size: 1326 4 995 5410
Fragments arranged by size:
5410 1326 995 4
BstBI TT'CG_AA
Cuts at: 6539 6539
Size: 7735
BstEII G'GTnAC_C
Cuts at: 1184 3944 1184
Size: 2760 4975
BstXI CCAn_nnnn'nTGG
Cuts at: 4049 4049
Size: 7735
BstYI r'GATC_y
Cuts at: 1972 3460 4128 5013 5065 5432 5817 5834 7286
Size: 1488 668 885 52 367 385 17 1452
Cuts at: 7286 7372 7384 7730 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1488 1452 885 668 385 367 346
86 52 17 12
Bsu36I CC'TnA_GG
Cuts at: 2261 2724 3078 2261
Size: 463 354 6918
Fragments arranged by size:
6918 463 354
BtgI C'CryG_G
Cuts at: 513 1065 2004 3780 513
Size: 552 939 1776 4468
Fragments arranged by size:
4468 1776 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6996
Fragments arranged by size:
6996 683 56
BtsI GCAGTG_nn'
Cuts at: 2038 2118 2169 3117 4303 5251 6045 6065 2038
Size: 80 51 948 1186 948 794 20 3708
Fragments arranged by size:
3708 1186 948 948 794 80 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7735
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7700
DraI TTT'AAA
Cuts at: 3441 5039 3441
Size: 1598 6137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6742 809
Size: 507 5426 1802
Fragments arranged by size:
5426 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4185 4977 5182
Size: 54 1425 50 275 18 2355 792 205
Cuts at: 5182 6071 7700 8
Size: 889 1629 43
Fragments arranged by size:
2355 1629 1425 889 792 275 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5182 1537
Size: 275 3370 4090
Fragments arranged by size:
4090 3370 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2493 2586 3605 4173 4647 4889 5665 1353
Size: 1140 93 1019 568 474 242 776 3423
Fragments arranged by size:
3423 1140 1019 776 568 474 242 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6322 6696 6842 387
Size: 1064 138 4733 374 146 1280
Fragments arranged by size:
4733 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2574 2690 2753 3060 3119 3275
Size: 393 783 159 116 63 307 59 156
Cuts at: 3275 3345 3669 3765 3896 3909 4144 4335 4866
Size: 70 324 96 131 13 235 191 531
Cuts at: 4866 4952 5798 6395 7182 7450 1239
Size: 86 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 783 597 531 393 324
307 268 235 191 159 156 131 116
96 86 70 63 59 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2691 4172 2691
Size: 1481 6254
EcoRI G'AATT_C
Cuts at: 4695 5044 5422 4695
Size: 349 378 7008
Fragments arranged by size:
7008 378 349
EcoRV GAT'ATC
Cuts at: 3334 4261 3334
Size: 927 6808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2597 3428 3482 2597
Size: 831 54 6850
Fragments arranged by size:
6850 831 54
FspI TGC'GCA
Cuts at: 6241 6241
Size: 7735
HaeII r_GCGC'y
Cuts at: 1678 4215 6882 1678
Size: 2537 2667 2531
Fragments arranged by size:
2667 2537 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2343 2375 3318 3350 3684
Size: 32 135 32 771 32 943 32 334
Cuts at: 3684 3716 4422 4454 6376 6408 6450 6482 1373
Size: 32 706 32 1922 32 42 32 2626
Fragments arranged by size:
2626 1922 943 771 706 334 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5079 5325 669
Size: 988 281 3141 246 3079
Fragments arranged by size:
3141 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3675 748
Size: 2927 4808
HpaI GTT'AAC
Cuts at: 5325 5325
Size: 7735
KpnI G_GTAC'C
Cuts at: 5060 5060
Size: 7735
MfeI C'AATT_G
Cuts at: 2562 4635 5334 2562
Size: 2073 699 4963
Fragments arranged by size:
4963 2073 699
MluI A'CGCG_T
Cuts at: 7611 7611
Size: 7735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2200 3472 3820 4554 6849 7033 1305
Size: 895 1272 348 734 2295 184 2007
Fragments arranged by size:
2295 2007 1272 895 734 348 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4187 4979 10
Size: 54 1425 343 2355 792 2766
Fragments arranged by size:
2766 2355 1425 792 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3867 5693 6052 6211 518
Size: 648 2701 1826 359 159 2042
Fragments arranged by size:
2701 2042 1826 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2465 2552 3341 3449 4972 5151 5828 6976
Size: 859 87 789 108 1523 179 677 1148
Cuts at: 6976 7221 1606
Size: 245 2120
Fragments arranged by size:
2120 1523 1148 859 789 677 245 179
108 87
NcoI C'CATG_G
Cuts at: 513 1065 2004 3780 513
Size: 552 939 1776 4468
Fragments arranged by size:
4468 1776 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7735
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7735
NotI GC'GGCC_GC
Cuts at: 5182 5182
Size: 7735
NspI r_CATG'y
Cuts at: 1132 1229 2791 3736 4028 4360 5093 6638 1132
Size: 97 1562 945 292 332 733 1545 2229
Fragments arranged by size:
2229 1562 1545 945 733 332 292 97
PasI CC'CwG_GG
Cuts at: 1348 4134 1348
Size: 2786 4949
PciI A'CATG_T
Cuts at: 3732 4024 6634 3732
Size: 292 2610 4833
Fragments arranged by size:
4833 2610 292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7735
PfoI T'CCnGG_A
Cuts at: 3037 3180 3037
Size: 143 7592
PmeI GTTT'AAAC
Cuts at: 5039 5039
Size: 7735
PmlI CAC'GTG
Cuts at: 4328 4328
Size: 7735
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5804 5836 7317 7349 5804
Size: 32 1481 32 6190
Fragments arranged by size:
6190 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7735
PsiI TTA'TAA
Cuts at: 2024 5305 2024
Size: 3281 4454
PstI C_TGCA'G
Cuts at: 830 2272 2408 4963 5087 830
Size: 1442 136 2555 124 3478
Fragments arranged by size:
3478 2555 1442 136 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7735
PvuII CAG'CTG
Cuts at: 2465 2552 3341 3449 4972 2465
Size: 87 789 108 1523 5228
Fragments arranged by size:
5228 1523 789 108 87
SalI G'TCGA_C
Cuts at: 1936 5077 1936
Size: 3141 4594
SbfI CC_TGCA'GG
Cuts at: 5087 5087
Size: 7735
ScaI AGT'ACT
Cuts at: 2019 3917 4486 5983 7695 2019
Size: 1898 569 1497 1712 2059
Fragments arranged by size:
2059 1898 1712 1497 569
SfcI C'TryA_G
Cuts at: 826 1045 2268 2404 3988 4959 5083 6218 6899
Size: 219 1223 136 1584 971 124 1135 681
Cuts at: 6899 7090 826
Size: 191 1471
Fragments arranged by size:
1584 1471 1223 1135 971 681 219 191
136 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7735
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7735
SmaI CCC'GGG
Cuts at: 1872 5062 1872
Size: 3190 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3084 3203 5839 6740 7002 820
Size: 197 926 1141 119 2636 901 262 1553
Fragments arranged by size:
2636 1553 1141 926 901 262 197 119
SnaBI TAC'GTA
Cuts at: 493 5526 493
Size: 5033 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7735
SphI G_CATG'C
Cuts at: 1132 2791 4360 5093 1132
Size: 1659 1569 733 3774
Fragments arranged by size:
3774 1659 1569 733
SspI AAT'ATT
Cuts at: 5 52 4953 5659 5
Size: 47 4901 706 2081
Fragments arranged by size:
4901 2081 706 47
StuI AGG'CCT
Cuts at: 1517 2504 1517
Size: 987 6748
StyI C'CwwG_G
Cuts at: 513 1065 2004 3051 3703 3780 5174 513
Size: 552 939 1047 652 77 1394 3074
Fragments arranged by size:
3074 1394 1047 939 652 552 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4276 4764 4812 5762 5779 5932 6117
Size: 1255 2696 488 48 950 17 153 185
Cuts at: 6117 6562 325
Size: 445 1498
Fragments arranged by size:
2696 1498 1255 950 488 445 185 153
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2017 2515 3915
Size: 274 80 33 51 1443 40 498 1400
Cuts at: 3915 4484 4831 4990 5981 7693 96
Size: 569 347 159 991 1712 138
Fragments arranged by size:
1712 1443 1400 991 569 498 347 274
159 138 80 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2460 2492 2580 2612 3087 3119 7695 7727 2460
Size: 32 88 32 475 32 4576 32 2468
Fragments arranged by size:
4576 2468 475 88 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4643 4670 1316
Size: 3327 27 4381
Fragments arranged by size:
4381 3327 27
XbaI T'CTAG_A
Cuts at: 2863 5071 2863
Size: 2208 5527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2370 4049 4724 2341
Size: 29 1679 675 5352
Fragments arranged by size:
5352 1679 675 29
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7735
XmaI C'CCGG_G
Cuts at: 1870 5060 1870
Size: 3190 4545
XmnI GAAnn'nnTTC
Cuts at: 2381 2577 2940 3847 4699 4884 5864 2381
Size: 196 363 907 852 185 980 4252
Fragments arranged by size:
4252 980 907 852 363 196 185
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5543 276
Size: 53 83 186 936 4009 2468
Fragments arranged by size:
4009 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI