Restriction map for
pFN21AB3008
(Circular) (Six-base) MAPSORT of: pFN21AB3008.seq Check: 6621 from: 1 to: 7171
pFN21AB3008 7171 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4981 278
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2629 4514 1937
Size: 692 1885 4594
Fragments arranged by size:
4594 1885 692
Acc65I G'GTAC_C
Cuts at: 4492 4492
Size: 7171
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3059 4329 5234 6618 6886 1632
Size: 1427 1270 905 1384 268 1917
Fragments arranged by size:
1917 1427 1384 1270 905 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6974
AflIII A'CryG_T
Cuts at: 1203 3968 6070 7047 1203
Size: 2765 2102 977 1327
Fragments arranged by size:
2765 2102 1327 977
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 4104 5900 4104
Size: 1796 5375
AleI CACnn'nnGTG
Cuts at: 1166 2458 2681 1166
Size: 1292 223 5656
Fragments arranged by size:
5656 1292 223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2476 4025 1184
Size: 1292 1549 4330
Fragments arranged by size:
4330 1549 1292
AlwNI CAG_nnn'CTG
Cuts at: 3380 3782 3899 3935 4914 6486 3380
Size: 402 117 36 979 1572 4065
Fragments arranged by size:
4065 1572 979 402 117 36
ApaLI G'TGCA_C
Cuts at: 4260 5228 6384 4260
Size: 968 1156 5047
Fragments arranged by size:
5047 1156 968
ApoI r'AATT_y
Cuts at: 1493 2383 2846 2984 4127 4480 4706 4858 6967
Size: 890 463 138 1143 353 226 152 2109
Cuts at: 6967 1493
Size: 1697
Fragments arranged by size:
2109 1697 1143 890 463 353 226 152
138
AseI AT'TA_AT
Cuts at: 160 1032 5725 160
Size: 872 4693 1606
Fragments arranged by size:
4693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 4496 1870
Size: 73 2553 4545
Fragments arranged by size:
4545 2553 73
BamHI G'GATC_C
Cuts at: 3506 4501 4868 3506
Size: 995 367 5809
Fragments arranged by size:
5809 995 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4492 5947 618
Size: 325 217 627 2705 1455 1842
Fragments arranged by size:
2705 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2650 3321 3654 4490 1391
Size: 1259 671 333 836 4072
Fragments arranged by size:
4072 1259 836 671 333
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4202 4405 928
Size: 971 2303 203 3694
Fragments arranged by size:
3694 2303 971 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3458 3492 5362 5396 1903
Size: 34 1521 34 1870 34 3678
Fragments arranged by size:
3678 1870 1521 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5063 6273 5063
Size: 1210 5961
BclI T'GATC_A
Cuts at: 1548 2247 1548
Size: 699 6472
BfrBI ATG'CAT
Cuts at: 2425 2425
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5782 136
Size: 107 122 71 631 605 4110 1525
Fragments arranged by size:
4110 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2488 3358 7166 2488
Size: 870 3808 2493
Fragments arranged by size:
3808 2493 870
BlpI GC'TnA_GC
Cuts at: 4588 4588
Size: 7171
Bme1580I G_kGCm'C
Cuts at: 1790 4264 5232 6388 1790
Size: 2474 968 1156 2573
Fragments arranged by size:
2573 2474 1156 968
BmgBI CAC'GTC
Cuts at: 1318 3971 4200 1318
Size: 2653 229 4289
Fragments arranged by size:
4289 2653 229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 4902 5860
Size: 416 53 83 399 24 608 2877 958
Cuts at: 5860 442
Size: 1753
Fragments arranged by size:
2877 1753 958 608 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4134 4134
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2399 3759 3912 3970 5831 1239
Size: 1160 1360 153 58 1861 2579
Fragments arranged by size:
2579 1861 1360 1160 153 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2295 5296 6161 6459 2295
Size: 3001 865 298 3007
Fragments arranged by size:
3007 3001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3763 5834 882
Size: 617 2264 2071 2219
Fragments arranged by size:
2264 2219 2071 617
BsaAI yAC'GTr
Cuts at: 493 1204 4962 493
Size: 711 3758 2702
Fragments arranged by size:
3758 2702 711
BsaBI GATnn'nnATC
Cuts at: 2493 3363 7165 2493
Size: 870 3802 2499
Fragments arranged by size:
3802 2499 870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2595 4978 5360 5995
Size: 53 83 186 936 1062 2383 382 635
Cuts at: 5995 275
Size: 1451
Fragments arranged by size:
2383 1451 1062 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4039 5604 6002 6276 6423 6834 4039
Size: 1565 398 274 147 411 4376
Fragments arranged by size:
4376 1565 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2931 2961 3880 3910 3968
Size: 30 165 30 1528 30 919 30 58
Cuts at: 3968 3998 1178
Size: 30 4351
Fragments arranged by size:
4351 1528 919 165 58 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3873 1186
Size: 2687 4484
BseYI C'CCAG_C
Cuts at: 3312 3955 6374 6870 3312
Size: 643 2419 496 3613
Fragments arranged by size:
3613 2419 643 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3131 1163
Size: 573 1395 5203
Fragments arranged by size:
5203 1395 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4621 5382 6410 1540
Size: 275 186 2620 761 1028 2301
Fragments arranged by size:
2620 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2650 3654 4264 5232 5317 6388 2650
Size: 1004 610 968 85 1071 3433
Fragments arranged by size:
3433 1071 1004 968 610 85
BsmI GAATG_Cn'
Cuts at: 2425 3439 4682 4775 2425
Size: 1014 1243 93 4821
Fragments arranged by size:
4821 1243 1014 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2650 3321 3654 4264 4490 5232 5317
Size: 399 860 671 333 610 226 742 85
Cuts at: 5317 6388 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 860 742 671 610 399 333
226 85
BspEI T'CCGG_A
Cuts at: 6834 6834
Size: 7171
BspHI T'CATG_A
Cuts at: 2761 5060 6790 2761
Size: 2299 1730 3142
Fragments arranged by size:
3142 2299 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4526 844
Size: 3682 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4118 5058 1394
Size: 429 2295 940 3507
Fragments arranged by size:
3507 2295 940 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3313 3707 5666 5840 65
Size: 1183 2065 394 1959 174 1396
Fragments arranged by size:
2065 1959 1396 1183 394 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5815 6002 1700
Size: 110 4005 187 2869
Fragments arranged by size:
4005 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4339 96
Size: 4243 2928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 5231 6243 5231
Size: 1012 6159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4150 794
Size: 3356 3815
BstBI TT'CG_AA
Cuts at: 3715 5975 3715
Size: 2260 4911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7171
BstYI r'GATC_y
Cuts at: 1972 2488 3358 3506 4501 4868 5253 5270 6722
Size: 516 870 148 995 367 385 17 1452
Cuts at: 6722 6808 6820 7166 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 995 870 516 385 367 346
148 86 17 12
BstZ17I GTA'TAC
Cuts at: 2630 2630
Size: 7171
Bsu36I CC'TnA_GG
Cuts at: 2600 2600
Size: 7171
BtgI C'CryG_G
Cuts at: 513 1065 4139 513
Size: 552 3074 3545
Fragments arranged by size:
3545 3074 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6432
Fragments arranged by size:
6432 683 56
BtsI GCAGTG_nn'
Cuts at: 2182 2407 4372 4687 5481 5501 2182
Size: 225 1965 315 794 20 3852
Fragments arranged by size:
3852 1965 794 315 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7136
DraI TTT'AAA
Cuts at: 2394 4475 2394
Size: 2081 5090
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6178 809
Size: 507 4862 1802
Fragments arranged by size:
4862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3030 3263 4618
Size: 54 1425 50 275 18 1200 233 1355
Cuts at: 4618 5507 7136 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1355 1200 889 275 233 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4618 1537
Size: 275 2806 4090
Fragments arranged by size:
4090 2806 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 2576 5101 1353
Size: 1151 72 2525 3423
Fragments arranged by size:
3423 2525 1151 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5758 6132 6278 387
Size: 1064 138 4169 374 146 1280
Fragments arranged by size:
4169 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2648 3652 2648
Size: 1004 6167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 3059 3759 3912 3970 4329 5234
Size: 393 767 660 700 153 58 359 905
Cuts at: 5234 5831 6618 6886 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 905 787 767 700 660 597 393
359 268 153 58
EcoO109I rG'GnC_Cy
Cuts at: 2131 3125 3952 2131
Size: 994 827 5350
Fragments arranged by size:
5350 994 827
EcoRI G'AATT_C
Cuts at: 4480 4858 4480
Size: 378 6793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 7171
FspI TGC'GCA
Cuts at: 5677 5677
Size: 7171
HaeII r_GCGC'y
Cuts at: 1678 6318 1678
Size: 4640 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 4090 4122 5812
Size: 32 135 32 776 32 1710 32 1690
Cuts at: 5812 5844 5886 5918 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1710 1690 776 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4515 4761 669
Size: 988 281 2577 246 3079
Fragments arranged by size:
3079 2577 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7171
HpaI GTT'AAC
Cuts at: 4761 4761
Size: 7171
KpnI G_GTAC'C
Cuts at: 4496 4496
Size: 7171
MfeI C'AATT_G
Cuts at: 3174 4770 3174
Size: 1596 5575
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3911 4088 6285 6469 1305
Size: 2606 177 2197 184 2007
Fragments arranged by size:
2606 2197 2007 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3032 3265 10
Size: 54 1425 343 1200 233 3916
Fragments arranged by size:
3916 1425 1200 343 233 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2458 2681 3444 5129 5488 5647 518
Size: 648 1292 223 763 1685 359 159 2042
Fragments arranged by size:
2042 1685 1292 763 648 359 223 159
MspA1I CmG'CkG
Cuts at: 1606 3217 3857 4587 5264 6412 6657 1606
Size: 1611 640 730 677 1148 245 2120
Fragments arranged by size:
2120 1611 1148 730 677 640 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6619
NdeI CA'TA_TG
Cuts at: 387 3289 387
Size: 2902 4269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NsiI A_TGCA'T
Cuts at: 2427 2427
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 4529 6074 1132
Size: 97 3300 1545 2229
Fragments arranged by size:
3300 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2666 1348
Size: 1318 5853
PciI A'CATG_T
Cuts at: 6070 6070
Size: 7171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2833 3470 3782 1108
Size: 1725 637 312 4497
Fragments arranged by size:
4497 1725 637 312
PfoI T'CCnGG_A
Cuts at: 2226 2226
Size: 7171
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5240 5272 6753 6785 5240
Size: 32 1481 32 5626
Fragments arranged by size:
5626 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3125 3952 3125
Size: 827 6344
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 4741 4741
Size: 7171
PstI C_TGCA'G
Cuts at: 830 3070 4523 830
Size: 2240 1453 3478
Fragments arranged by size:
3478 2240 1453
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 3217 3857 3217
Size: 640 6531
SacI G_AGCT'C
Cuts at: 2650 3654 2650
Size: 1004 6167
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SbfI CC_TGCA'GG
Cuts at: 4523 4523
Size: 7171
ScaI AGT'ACT
Cuts at: 3147 5419 7131 3147
Size: 2272 1712 3187
Fragments arranged by size:
3187 2272 1712
SfcI C'TryA_G
Cuts at: 826 1045 3066 4324 4519 5654 6335 6526 826
Size: 219 2021 1258 195 1135 681 191 1471
Fragments arranged by size:
2021 1471 1258 1135 681 219 195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 4498 1872
Size: 2626 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2310 5275 6176 6438 820
Size: 197 926 367 2965 901 262 1553
Fragments arranged by size:
2965 1553 926 901 367 262 197
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7171
SphI G_CATG'C
Cuts at: 1132 4529 1132
Size: 3397 3774
SspI AAT'ATT
Cuts at: 5 52 5095 5
Size: 47 5043 2081
Fragments arranged by size:
5043 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7171
StyI C'CwwG_G
Cuts at: 513 1065 2292 2731 2750 3520 4610 513
Size: 552 1227 439 19 770 1090 3074
Fragments arranged by size:
3074 1227 1090 770 552 439 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2656 2693 4062 5198 5215 5368 5553
Size: 1255 1076 37 1369 1136 17 153 185
Cuts at: 5553 5998 325
Size: 445 1498
Fragments arranged by size:
1498 1369 1255 1136 1076 445 185 153
37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3145 4339 5417
Size: 274 80 33 51 1443 1168 1194 1078
Cuts at: 5417 7129 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1194 1168 1078 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3631 3663 7131 7163 3631
Size: 32 3468 32 3639
Fragments arranged by size:
3639 3468 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2168 1316
Size: 852 6319
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 4496 1870
Size: 2626 4545
XmnI GAAnn'nnTTC
Cuts at: 4127 5300 4127
Size: 1173 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4979 276
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI