Restriction map for
pFN21AB3002
(Circular) (Six-base) MAPSORT of: pFN21AB3002.seq Check: 799 from: 1 to: 6430
pFN21AB3002 6430 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3288 3773 1937
Size: 1351 485 4594
Fragments arranged by size:
4594 1351 485
Acc65I G'GTAC_C
Cuts at: 3060 3751 3060
Size: 691 5739
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2474 3155 3319 3667 4493 5877 6145
Size: 713 129 681 164 348 826 1384 268
Cuts at: 6145 1632
Size: 1917
Fragments arranged by size:
1917 1384 826 713 681 348 268 164
129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 3608 1166
Size: 2442 3988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 2702 4173 5745 2702
Size: 1471 1572 3387
Fragments arranged by size:
3387 1572 1471
ApaI G_GGCC'C
Cuts at: 2143 2237 2833 2143
Size: 94 596 5740
Fragments arranged by size:
5740 596 94
ApaLI G'TGCA_C
Cuts at: 2727 4487 5643 2727
Size: 1760 1156 3514
Fragments arranged by size:
3514 1760 1156
ApoI r'AATT_y
Cuts at: 1493 2049 3739 3965 4117 6226 1493
Size: 556 1690 226 152 2109 1697
Fragments arranged by size:
2109 1697 1690 556 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 2317 3755 1870
Size: 73 229 145 1438 4545
Fragments arranged by size:
4545 1438 229 145 73
AvrII C'CTAG_G
Cuts at: 2090 2090
Size: 6430
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3313 3346 3313
Size: 33 6397
BamHI G'GATC_C
Cuts at: 2008 3760 4127 2008
Size: 1752 367 4311
Fragments arranged by size:
4311 1752 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2471 2557 3060 3459 3465
Size: 325 217 627 684 86 503 399 6
Cuts at: 3465 3484 3751 5206 618
Size: 19 267 1455 1842
Fragments arranged by size:
1842 1455 684 627 503 399 325 267
217 86 19 6
BanII G_rGCy'C
Cuts at: 1391 2132 2143 2173 2237 2287 2833 3015 3559
Size: 741 11 30 64 50 546 182 544
Cuts at: 3559 3749 1391
Size: 190 4072
Fragments arranged by size:
4072 741 546 544 190 182 64 50
30 11
BbeI G_GCGC'C
Cuts at: 3469 3469
Size: 6430
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3093 3347 3673 928
Size: 971 1194 254 326 3685
Fragments arranged by size:
3685 1194 971 326 254
BbvCI CC'TCA_GC
Cuts at: 3599 3599
Size: 6430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4621 4655 1903
Size: 34 2684 34 3678
Fragments arranged by size:
3678 2684 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2918 4322 5532 2918
Size: 1404 1210 3816
Fragments arranged by size:
3816 1404 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BfrBI ATG'CAT
Cuts at: 2063 2063
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3454 5041 136
Size: 107 122 71 631 605 1782 1587 1525
Fragments arranged by size:
1782 1587 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 2126 2325 2348 3717 3847 2126
Size: 199 23 1369 130 4709
Fragments arranged by size:
4709 1369 199 130 23
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2237 2731 2833 3489 3511 4491 5647
Size: 353 94 494 102 656 22 980 1156
Cuts at: 5647 1790
Size: 2573
Fragments arranged by size:
2573 1156 980 656 494 353 102 94
22
BmgBI CAC'GTC
Cuts at: 1318 3292 1318
Size: 1974 4456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2403 3736 4161
Size: 416 53 83 399 24 986 1333 425
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1753 1333 986 958 425 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2975 5090 1239
Size: 1736 2115 2579
Fragments arranged by size:
2579 2115 1736
Bpu10I CC'TnA_GC
Cuts at: 3599 3599
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2722 2846 4555 5420 5718 2693
Size: 29 124 1709 865 298 3405
Fragments arranged by size:
3405 1709 865 298 124 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5093 882
Size: 617 3594 2219
Fragments arranged by size:
3594 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 4237 4619 5254
Size: 53 83 186 936 1933 771 382 635
Cuts at: 5254 275
Size: 1451
Fragments arranged by size:
1933 1451 936 771 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3615 4863 5261 5535 5682 6093 3615
Size: 1248 398 274 147 411 3952
Fragments arranged by size:
3952 1248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2651 2681 1178
Size: 30 165 30 1248 30 4927
Fragments arranged by size:
4927 1248 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2383 2496 2499 2502 2505 2526 3188 3413
Size: 1197 113 3 3 3 21 662 225
Cuts at: 3413 1186
Size: 4203
Fragments arranged by size:
4203 1197 662 225 113 21 3 3
3
BseYI C'CCAG_C
Cuts at: 2307 2435 2574 2695 3035 3117 5633 6129 2307
Size: 128 139 121 340 82 2516 496 2608
Fragments arranged by size:
2608 2516 496 340 139 128 121 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2646 3516 1163
Size: 573 910 870 4077
Fragments arranged by size:
4077 910 870 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3880 4641 5669 1540
Size: 275 186 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2132 2326 2731 3015 3297 4491 4576 5647 2132
Size: 194 405 284 282 1194 85 1071 2915
Fragments arranged by size:
2915 1194 1071 405 284 282 194 85
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2143 2173 2237 2287 2326 2731
Size: 399 342 11 30 64 50 39 405
Cuts at: 2731 2833 3015 3297 3489 3511 3559 3749 4491
Size: 102 182 282 192 22 48 190 742
Cuts at: 4491 4576 5647 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 342 282 192
190 182 102 85 64 50 48 39
30 22 11
BspEI T'CCGG_A
Cuts at: 3615 6093 3615
Size: 2478 3952
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3721 3785 844
Size: 2877 64 3489
Fragments arranged by size:
3489 2877 64
BsrBI CCG'CTC
Cuts at: 1394 1823 2961 3546 3721 4317 1394
Size: 429 1138 585 175 596 3507
Fragments arranged by size:
3507 1138 596 585 429 175
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3315 3435 3462 3524 5074 5261 1700
Size: 110 1505 120 27 62 1550 187 2869
Fragments arranged by size:
2869 1550 1505 187 120 110 62 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 2008 2949 3760 4127 4512 4529 5981 6067
Size: 36 941 811 367 385 17 1452 86
Cuts at: 6067 6079 6425 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 941 811 385 367 346 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3625 513
Size: 552 939 1621 3318
Fragments arranged by size:
3318 1621 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 3603 3946 4740 4760 3603
Size: 343 794 20 5273
Fragments arranged by size:
5273 794 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2653 2688 529
Size: 35 2089 35 4271
Fragments arranged by size:
4271 2089 35 35
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 2254 2254
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2212 2653 2793
Size: 54 1425 50 275 18 382 441 140
Cuts at: 2793 3313 3561 3646 3877 4766 6395 8
Size: 520 248 85 231 889 1629 43
Fragments arranged by size:
1629 1425 889 520 441 382 275 248
231 140 85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3877 1537
Size: 275 2065 4090
Fragments arranged by size:
4090 2065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2112 3020 4360 1353
Size: 741 18 908 1340 3423
Fragments arranged by size:
3423 1340 908 741 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 5017 5391 5537 387
Size: 1064 138 690 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 690 374 146 138
EcoICRI GAG'CTC
Cuts at: 2130 3013 2130
Size: 883 5547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2474 2975 3155 3319 3667 4493
Size: 393 713 129 501 180 164 348 826
Cuts at: 4493 5090 5877 6145 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 826 787 713 597 501 393 348
268 180 164 129
EcoO109I rG'GnC_Cy
Cuts at: 2233 2482 2829 2830 3641 2233
Size: 249 347 1 811 5022
Fragments arranged by size:
5022 811 347 249 1
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3697 3697
Size: 6430
FspI TGC'GCA
Cuts at: 2153 4936 2153
Size: 2783 3647
FspAI rTGC'GCAy
Cuts at: 2153 2153
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 3469 5577 1678
Size: 1791 2108 2531
Fragments arranged by size:
2531 2108 1791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2382 2414 3302
Size: 32 135 32 479 32 299 32 888
Cuts at: 3302 3334 5071 5103 5145 5177 1373
Size: 32 1737 32 42 32 2626
Fragments arranged by size:
2626 1737 888 479 299 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3289 3774 4020 669
Size: 988 281 1351 485 246 3079
Fragments arranged by size:
3079 1351 988 485 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KasI G'GCGC_C
Cuts at: 3465 3465
Size: 6430
KpnI G_GTAC'C
Cuts at: 3064 3755 3064
Size: 691 5739
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3045 5544 5728 1305
Size: 1740 2499 184 2007
Fragments arranged by size:
2499 2007 1740 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2655 2795 3563 10
Size: 54 1425 343 823 140 768 2877
Fragments arranged by size:
2877 1425 823 768 343 140 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2058 2255 2988 3144 3309 3479 3482
Size: 648 892 197 733 156 165 170 3
Cuts at: 3482 3608 4388 4747 4906 518
Size: 126 780 359 159 2042
Fragments arranged by size:
2042 892 780 733 648 359 197 170
165 159 156 126 3
MspA1I CmG'CkG
Cuts at: 1606 2311 2329 2630 3846 4523 5671 5916 1606
Size: 705 18 301 1216 677 1148 245 2120
Fragments arranged by size:
2120 1216 1148 705 677 301 245 18
NaeI GCC'GGC
Cuts at: 3437 3437
Size: 6430
NarI GG'CG_CC
Cuts at: 3466 3466
Size: 6430
NcoI C'CATG_G
Cuts at: 513 1065 2004 3625 513
Size: 552 939 1621 3318
Fragments arranged by size:
3318 1621 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 3435 3435
Size: 6430
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NsiI A_TGCA'T
Cuts at: 2065 2065
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3249 3596 3788 5333 1132
Size: 97 2020 347 192 1545 2229
Fragments arranged by size:
2229 2020 1545 347 192 97
PasI CC'CwG_GG
Cuts at: 1348 2013 2230 2833 2912 3168 3443 1348
Size: 665 217 603 79 256 275 4335
Fragments arranged by size:
4335 665 603 275 256 217 79
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3027 1108
Size: 1919 4511
PfoI T'CCnGG_A
Cuts at: 2709 2952 3153 2709
Size: 243 201 5986
Fragments arranged by size:
5986 243 201
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3582 3614 4499 4531 6012 6044 3582
Size: 32 885 32 1481 32 3968
Fragments arranged by size:
3968 1481 885 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2139 2233 2829 2139
Size: 94 596 5740
Fragments arranged by size:
5740 596 94
PstI C_TGCA'G
Cuts at: 830 2906 3782 830
Size: 2076 876 3478
Fragments arranged by size:
3478 2076 876
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2311 2329 2630 2311
Size: 18 301 6111
Fragments arranged by size:
6111 301 18
SacI G_AGCT'C
Cuts at: 2132 3015 2132
Size: 883 5547
SalI G'TCGA_C
Cuts at: 1936 3287 3772 1936
Size: 1351 485 4594
Fragments arranged by size:
4594 1351 485
SapI GCTCTTCn'nnn_
Cuts at: 3020 3020
Size: 6430
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 2076 3422 4678 6390 2076
Size: 1346 1256 1712 2116
Fragments arranged by size:
2116 1712 1346 1256
SfcI C'TryA_G
Cuts at: 826 1045 2803 2902 3180 3490 3778 4913 5594
Size: 219 1758 99 278 310 288 1135 681
Cuts at: 5594 5785 826
Size: 191 1471
Fragments arranged by size:
1758 1471 1135 681 310 288 278 219
191 99
SfoI GGC'GCC
Cuts at: 3467 3467
Size: 6430
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 3462 1700
Size: 1762 4668
SmaI CCC'GGG
Cuts at: 1872 2174 2319 3757 1872
Size: 302 145 1438 4545
Fragments arranged by size:
4545 1438 302 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2672 2737 2861 4534 5435 5697
Size: 197 926 729 65 124 1673 901 262
Cuts at: 5697 820
Size: 1553
Fragments arranged by size:
1673 1553 926 901 729 262 197 124
65
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SrfI GCCC'GGGC
Cuts at: 2174 2174
Size: 6430
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 2527 2900 3104 3218 3696 1517
Size: 1010 373 204 114 478 4251
Fragments arranged by size:
4251 1010 478 373 204 114
StyI C'CwwG_G
Cuts at: 513 1065 2004 2090 2844 3570 3625 3869 513
Size: 552 939 86 754 726 55 244 3074
Fragments arranged by size:
3074 939 754 726 552 244 86 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 2582 3321 3638 4457 4474 4627
Size: 1255 909 93 739 317 819 17 153
Cuts at: 4627 4812 5257 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 909 819 739 445 317 185
153 93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 3420 4676
Size: 274 80 33 51 1443 97 1346 1256
Cuts at: 4676 6388 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1346 1256 274 138 97 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6390 6422 6390
Size: 32 6398
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 3401 3556 2416
Size: 985 155 5290
Fragments arranged by size:
5290 985 155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 2172 2317 3755 1870
Size: 302 145 1438 4545
Fragments arranged by size:
4545 1438 302 145
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoNI EcoRV FseI NruI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI