Restriction map for
pFN21AB2501
(Circular) (Six-base) MAPSORT of: pFN21AB2501.seq Check: 6932 from: 1 to: 6619
pFN21AB2501 6619 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3962 1937
Size: 2025 4594
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3228 4682 6066 6334 1632
Size: 1596 1454 1384 268 1917
Fragments arranged by size:
1917 1596 1454 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 2388 3575 3725 3753 3755 5518 6495 1203
Size: 1185 1187 150 28 2 1763 977 1327
Fragments arranged by size:
1763 1327 1187 1185 977 150 28 2
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2407 1184
Size: 1223 5396
AlwNI CAG_nnn'CTG
Cuts at: 3413 4362 5934 3413
Size: 949 1572 4098
Fragments arranged by size:
4098 1572 949
ApaI G_GGCC'C
Cuts at: 2606 2656 2606
Size: 50 6569
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 2891 2930 3075 3146 3928 4154 4306 6415
Size: 1398 39 145 71 782 226 152 2109
Cuts at: 6415 1493
Size: 1697
Fragments arranged by size:
2109 1697 1398 782 226 152 145 71
39
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 2219 2326 2597 3944 1870
Size: 73 276 107 271 1347 4545
Fragments arranged by size:
4545 1347 276 271 107 73
BamHI G'GATC_C
Cuts at: 2839 3066 3949 4316 2839
Size: 227 883 367 5142
Fragments arranged by size:
5142 883 367 227
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2322 3940 5395 618
Size: 325 217 627 264 271 1618 1455 1842
Fragments arranged by size:
1842 1618 1455 627 325 271 264 217
BanII G_rGCy'C
Cuts at: 1391 2130 2454 2598 2606 2656 3229 3370 3938
Size: 739 324 144 8 50 573 141 568
Cuts at: 3938 1391
Size: 4072
Fragments arranged by size:
4072 739 573 568 324 144 141 50
8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2326 2670 928
Size: 971 427 344 4877
Fragments arranged by size:
4877 971 427 344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4810 4844 1903
Size: 34 2873 34 3678
Fragments arranged by size:
3678 2873 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2495 2610 5230
Size: 107 122 71 631 605 823 115 2620
Cuts at: 5230 136
Size: 1525
Fragments arranged by size:
2620 1525 823 631 605 122 115 107
71
BglII A'GATC_T
Cuts at: 6614 6614
Size: 6619
BlpI GC'TnA_GC
Cuts at: 4036 4036
Size: 6619
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2606 2656 4680 5836 1790
Size: 264 552 50 2024 1156 2573
Fragments arranged by size:
2573 2024 1156 552 264 50
BmgBI CAC'GTC
Cuts at: 1318 2389 2446 1318
Size: 1071 57 5491
Fragments arranged by size:
5491 1071 57
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4350 5308 442
Size: 416 53 83 399 24 2933 958 1753
Fragments arranged by size:
2933 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3864 3909 3864
Size: 45 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2647 3405 3834 5279 1239
Size: 1408 758 429 1445 2579
Fragments arranged by size:
2579 1445 1408 758 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 4744 5609 5907 2066
Size: 2678 865 298 2778
Fragments arranged by size:
2778 2678 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2432 2507 5282 882
Size: 617 933 75 2775 2219
Fragments arranged by size:
2775 2219 933 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 3756 4410 493
Size: 711 2552 654 2702
Fragments arranged by size:
2702 2552 711 654
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2331 2487 4426 4808
Size: 53 83 186 936 798 156 1939 382
Cuts at: 4808 5443 275
Size: 635 1451
Fragments arranged by size:
1939 1451 936 798 635 382 186 156
83 53
BsaWI w'CCGG_w
Cuts at: 5052 5450 5724 5871 6282 5052
Size: 398 274 147 411 5389
Fragments arranged by size:
5389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2856 2886 1178
Size: 30 165 30 1453 30 4911
Fragments arranged by size:
4911 1453 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2110 2423 2426 2507 2644 3495 1186
Size: 924 313 3 81 137 851 4310
Fragments arranged by size:
4310 924 851 313 137 81 3
BseYI C'CCAG_C
Cuts at: 2572 5822 6318 2572
Size: 3250 496 2873
Fragments arranged by size:
3250 2873 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 1163
Size: 573 621 5425
Fragments arranged by size:
5425 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2302 2320 4069 4830 5858 1540
Size: 275 186 301 18 1749 761 1028 2301
Fragments arranged by size:
2301 1749 1028 761 301 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2349 3229 3258 3379 4680 4765 5836 2349
Size: 880 29 121 1301 85 1071 3132
Fragments arranged by size:
3132 1301 1071 880 121 85 29
BsmI GAATG_Cn'
Cuts at: 4130 4223 4130
Size: 93 6526
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2130 2349 2454 2598 2606 2656
Size: 399 264 76 219 105 144 8 50
Cuts at: 2656 3229 3258 3370 3379 3938 4680 4765 5836
Size: 573 29 112 9 559 742 85 1071
Cuts at: 5836 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 573 559 399 264 219
144 112 105 85 76 50 29 9
8
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 4508 6238 4508
Size: 1730 4889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3974 844
Size: 3130 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2401 4506 1394
Size: 429 578 2105 3507
Fragments arranged by size:
3507 2105 578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3459 3838 5114 5288 65
Size: 1183 2211 379 1276 174 1396
Fragments arranged by size:
2211 1396 1276 1183 379 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2319 2363 2472 5263 5450 1700
Size: 110 509 44 109 2791 187 2869
Fragments arranged by size:
2869 2791 509 187 110 109 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3833 794
Size: 3039 3580
BstBI TT'CG_AA
Cuts at: 2661 5423 2661
Size: 2762 3857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstXI CCAn_nnnn'nTGG
Cuts at: 2294 2572 2294
Size: 278 6341
BstYI r'GATC_y
Cuts at: 1972 2839 3066 3949 4316 4701 4718 6170 6256
Size: 867 227 883 367 385 17 1452 86
Cuts at: 6256 6268 6614 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 883 867 385 367 346 227
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3044 3507 3044
Size: 463 6156
BtgI C'CryG_G
Cuts at: 513 1065 2004 2148 2266 513
Size: 552 939 144 118 4866
Fragments arranged by size:
4866 939 552 144 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 2374 4135 4929 4949 2374
Size: 1761 794 20 4044
Fragments arranged by size:
4044 1761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 3569 3569
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2299 2562 2620
Size: 54 1425 50 275 18 469 263 58
Cuts at: 2620 2967 4066 4955 6584 8
Size: 347 1099 889 1629 43
Fragments arranged by size:
1629 1425 1099 889 469 347 275 263
58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2299 4066 1537
Size: 275 487 1767 4090
Fragments arranged by size:
4090 1767 487 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2408 2663 3132 3709 4549 1353
Size: 1055 255 469 577 840 3423
Fragments arranged by size:
3423 1055 840 577 469 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3227 3227
Size: 6619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2647 3228 3405 3834 4682 5279 6066
Size: 393 1015 581 177 429 848 597 787
Cuts at: 6066 6334 1239
Size: 268 1524
Fragments arranged by size:
1524 1015 848 787 597 581 429 393
268 177
EcoO109I rG'GnC_Cy
Cuts at: 2554 2602 2603 2653 2554
Size: 48 1 50 6520
Fragments arranged by size:
6520 50 48 1
EcoRI G'AATT_C
Cuts at: 2930 3928 4306 2930
Size: 998 378 5243
Fragments arranged by size:
5243 998 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3050 3050
Size: 6619
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 2207 2501 3892 5766 1678
Size: 529 294 1391 1874 2531
Fragments arranged by size:
2531 1874 1391 529 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 2657 2689 3888
Size: 32 135 32 1028 32 25 32 1199
Cuts at: 3888 3920 5260 5292 5334 5366 1373
Size: 32 1340 32 42 32 2626
Fragments arranged by size:
2626 1340 1199 1028 135 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3963 4209 669
Size: 988 281 2025 246 3079
Fragments arranged by size:
3079 2025 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2760 3802 748
Size: 2012 1042 3565
Fragments arranged by size:
3565 2012 1042
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5733 5917 1305
Size: 4428 184 2007
Fragments arranged by size:
4428 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2969 10
Size: 54 1425 343 1137 3660
Fragments arranged by size:
3660 1425 1137 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 3359 3407 4577 4936 5095 518
Size: 648 1126 1067 48 1170 359 159 2042
Fragments arranged by size:
2042 1170 1126 1067 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2212 2268 2625 4035 4712 5860 6105 1606
Size: 606 56 357 1410 677 1148 245 2120
Fragments arranged by size:
2120 1410 1148 677 606 357 245 56
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5128
Fragments arranged by size:
5128 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6619
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 2299 4066 2299
Size: 1767 4852
NspI r_CATG'y
Cuts at: 1132 1229 2650 3358 3579 3729 3977 5522 1132
Size: 97 1421 708 221 150 248 1545 2229
Fragments arranged by size:
2229 1545 1421 708 248 221 150 97
PasI CC'CwG_GG
Cuts at: 1348 3316 1348
Size: 1968 4651
PciI A'CATG_T
Cuts at: 3575 3725 5518 3575
Size: 150 1793 4676
Fragments arranged by size:
4676 1793 150
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PmlI CAC'GTG
Cuts at: 3756 3756
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4688 4720 6201 6233 4688
Size: 32 1481 32 5074
Fragments arranged by size:
5074 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2554 2554
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 3576 1613
Size: 1963 4656
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PspOMI G'GGCC_C
Cuts at: 2602 2652 2602
Size: 50 6569
PstI C_TGCA'G
Cuts at: 830 2169 2261 2640 3971 830
Size: 1339 92 379 1331 3478
Fragments arranged by size:
3478 1339 1331 379 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
SacI G_AGCT'C
Cuts at: 3229 3229
Size: 6619
SacII CC_GC'GG
Cuts at: 2269 2269
Size: 6619
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SapI GCTCTTCn'nnn_
Cuts at: 2408 2408
Size: 6619
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 3139 4867 6579 3139
Size: 1728 1712 3179
Fragments arranged by size:
3179 1728 1712
SfcI C'TryA_G
Cuts at: 826 1045 2165 2257 2636 2819 3297 3967 5102
Size: 219 1120 92 379 183 478 670 1135
Cuts at: 5102 5783 5974 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 670 478 379 219
191 183 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 2221 2328 3946 1872
Size: 349 107 1618 4545
Fragments arranged by size:
4545 1618 349 107
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 4723 5624 5886 820
Size: 197 926 138 2642 901 262 1553
Fragments arranged by size:
2642 1553 926 901 262 197 138
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3358 3977 1132
Size: 2226 619 3774
Fragments arranged by size:
3774 2226 619
SspI AAT'ATT
Cuts at: 5 52 2770 4543 5
Size: 47 2718 1773 2081
Fragments arranged by size:
2718 2081 1773 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6619
StyI C'CwwG_G
Cuts at: 513 1065 2004 3447 3473 4058 513
Size: 552 939 1443 26 585 3074
Fragments arranged by size:
3074 1443 939 585 552 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 4646 4663 4816 5001 5446 325
Size: 1255 876 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 876 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3137 3438 3560
Size: 274 80 33 51 1443 1160 301 122
Cuts at: 3560 3638 4865 6577 96
Size: 78 1227 1712 138
Fragments arranged by size:
1712 1443 1227 1160 301 274 138 122
80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2856 2888 6579 6611 2856
Size: 32 3691 32 2864
Fragments arranged by size:
3691 2864 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6619
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3313 3313
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 2219 2326 3944 1870
Size: 349 107 1618 4545
Fragments arranged by size:
4545 1618 349 107
XmnI GAAnn'nnTTC
Cuts at: 4748 4748
Size: 6619
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspXI PsrI PvuII RsrII SanDI SexAI SfiI
SfoI SrfI SwaI