Restriction map for
pFN21AB2323
(Circular) (Six-base) MAPSORT of: pFN21AB2323.seq Check: 3657 from: 1 to: 7768
pFN21AB2323 7768 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3166 4656 3166
Size: 1490 6278
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2286 5578 278
Size: 53 83 186 936 750 3292 2468
Fragments arranged by size:
3292 2468 936 750 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 2642 3251 4361 5111 1937
Size: 349 356 609 1110 750 4594
Fragments arranged by size:
4594 1110 750 609 356 349
Acc65I G'GTAC_C
Cuts at: 2556 3433 5089 2556
Size: 877 1656 5235
Fragments arranged by size:
5235 1656 877
AclI AA'CG_TT
Cuts at: 1563 5067 5895 6268 1563
Size: 3504 828 373 3063
Fragments arranged by size:
3504 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2910 3035 3167 4109 4706 4809 5831
Size: 495 783 125 132 942 597 103 1022
Cuts at: 5831 7215 7483 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1022 942 783 597 495 268
132 125 103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7571
AflIII A'CryG_T
Cuts at: 1203 4444 6667 7644 1203
Size: 3241 2223 977 1327
Fragments arranged by size:
3241 2223 1327 977
AgeI A'CCGG_T
Cuts at: 6599 6599
Size: 7768
AhdI GACnn_n'nnGTC
Cuts at: 6497 6497
Size: 7768
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7768
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3435 1184
Size: 2251 5517
AlwNI CAG_nnn'CTG
Cuts at: 3602 4851 5511 7083 3602
Size: 1249 660 1572 4287
Fragments arranged by size:
4287 1572 1249 660
ApaI G_GGCC'C
Cuts at: 2637 3110 2637
Size: 473 7295
ApaLI G'TGCA_C
Cuts at: 3212 4592 5825 6981 3212
Size: 1380 1233 1156 3999
Fragments arranged by size:
3999 1380 1233 1156
ApoI r'AATT_y
Cuts at: 1493 3817 3946 4039 4253 5077 5303 5455 7564
Size: 2324 129 93 214 824 226 152 2109
Cuts at: 7564 1493
Size: 1697
Fragments arranged by size:
2324 2109 1697 824 226 214 152 129
93
AseI AT'TA_AT
Cuts at: 160 1032 4776 6322 160
Size: 872 3744 1546 1606
Fragments arranged by size:
3744 1606 1546 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7768
AvaI C'yCGr_G
Cuts at: 1870 1943 3374 3970 5093 1870
Size: 73 1431 596 1123 4545
Fragments arranged by size:
4545 1431 1123 596 73
BamHI G'GATC_C
Cuts at: 2773 2832 5098 5465 2773
Size: 59 2266 367 5076
Fragments arranged by size:
5076 2266 367 59
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2556 3122 3419 3433 3721
Size: 325 217 627 769 566 297 14 288
Cuts at: 3721 4644 5089 6544 618
Size: 923 445 1455 1842
Fragments arranged by size:
1842 1455 923 769 627 566 445 325
297 288 217 14
BanII G_rGCy'C
Cuts at: 1391 2637 3054 3110 4065 4638 4833 5087 1391
Size: 1246 417 56 955 573 195 254 4072
Fragments arranged by size:
4072 1246 955 573 417 254 195 56
BbeI G_GCGC'C
Cuts at: 3126 3126
Size: 7768
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3848 4317 5049 928
Size: 971 1949 469 732 3647
Fragments arranged by size:
3647 1949 971 732 469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5959 5993 1903
Size: 34 4022 34 3678
Fragments arranged by size:
4022 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5660 6870 5660
Size: 1210 6558
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7768
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6379 136
Size: 107 122 71 631 605 4707 1525
Fragments arranged by size:
4707 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3093 3571 7763 3093
Size: 478 4192 3098
Fragments arranged by size:
4192 3098 478
BlpI GC'TnA_GC
Cuts at: 2054 4528 5185 2054
Size: 2474 657 4637
Fragments arranged by size:
4637 2474 657
Bme1580I G_kGCm'C
Cuts at: 1790 2637 3110 3216 4596 5829 6985 1790
Size: 847 473 106 1380 1233 1156 2573
Fragments arranged by size:
2573 1380 1233 1156 847 473 106
BmgBI CAC'GTC
Cuts at: 1318 3738 1318
Size: 2420 5348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2111 2693 3432
Size: 416 53 83 399 24 694 582 739
Cuts at: 3432 3484 4227 5499 6457 442
Size: 52 743 1272 958 1753
Fragments arranged by size:
1753 1272 958 743 739 694 582 416
399 83 53 52 24
BmtI G_CTAG'C
Cuts at: 1056 3759 1056
Size: 2703 5065
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3600 3308
Size: 292 7476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2705 3038 3482 3512 3635 4208 4532
Size: 1082 384 333 444 30 123 573 324
Cuts at: 4532 4637 5037 6428 1239
Size: 105 400 1391 2579
Fragments arranged by size:
2579 1391 1082 573 444 400 384 333
324 123 105 30
Bpu10I CC'TnA_GC
Cuts at: 4637 4637
Size: 7768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3074 3701 4406 5893 6758 7056 3074
Size: 627 705 1487 865 298 3786
Fragments arranged by size:
3786 1487 865 705 627 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6431 882
Size: 617 4932 2219
Fragments arranged by size:
4932 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2290 3211 4445 5559 493
Size: 711 1086 921 1234 1114 2702
Fragments arranged by size:
2702 1234 1114 1086 921 711
BsaBI GATnn'nnATC
Cuts at: 2795 7762 2795
Size: 4967 2801
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 3123 5575 5957
Size: 53 83 186 936 750 840 2452 382
Cuts at: 5957 6592 275
Size: 635 1451
Fragments arranged by size:
2452 1451 936 840 750 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6201 6599 6873 7020 7431 6201
Size: 398 274 147 411 6538
Fragments arranged by size:
6538 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7543
Fragments arranged by size:
7543 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2589 4846 4895 4916 5063 1186
Size: 1403 2257 49 21 147 3891
Fragments arranged by size:
3891 2257 1403 147 49 21
BseYI C'CCAG_C
Cuts at: 3415 6971 7467 3415
Size: 3556 496 3716
Fragments arranged by size:
3716 3556 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3431 3556 4277 4634 1163
Size: 573 1695 125 721 357 4297
Fragments arranged by size:
4297 1695 721 573 357 125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4321 5218 5979 7007 1540
Size: 275 186 2320 897 761 1028 2301
Fragments arranged by size:
2320 2301 1028 897 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3054 3216 4065 4485 4596 4812 5829 5914 6985
Size: 162 849 420 111 216 1017 85 1071
Cuts at: 6985 3054
Size: 3837
Fragments arranged by size:
3837 1071 1017 849 420 216 162 111
85
BsmI GAATG_Cn'
Cuts at: 5279 5372 5279
Size: 93 7675
BsmBI CGTCTCn'nnnn_
Cuts at: 2274 2274
Size: 7768
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2637 3054 3110 3216 4065 4485 4596
Size: 399 847 417 56 106 849 420 111
Cuts at: 4596 4638 4812 4833 5087 5829 5914 6985 1391
Size: 42 174 21 254 742 85 1071 2174
Fragments arranged by size:
2174 1071 849 847 742 420 417 399
254 174 111 106 85 56 42 21
BspEI T'CCGG_A
Cuts at: 7431 7431
Size: 7768
BspHI T'CATG_A
Cuts at: 3150 5657 7387 3150
Size: 2507 1730 3531
Fragments arranged by size:
3531 2507 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 3166 4656 5123 844
Size: 1352 970 1490 467 3489
Fragments arranged by size:
3489 1490 1352 970 467
BsrBI CCG'CTC
Cuts at: 1394 1823 2650 5655 1394
Size: 429 827 3005 3507
Fragments arranged by size:
3507 3005 827 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2140 6263 6437 65
Size: 1183 892 4123 174 1396
Fragments arranged by size:
4123 1396 1183 892 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3701 6412 6599 1700
Size: 110 1891 2711 187 2869
Fragments arranged by size:
2869 2711 1891 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7768
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7768
BssSI C'ACGA_G
Cuts at: 5828 6840 5828
Size: 1012 6756
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2130 2269 794
Size: 1336 139 6293
Fragments arranged by size:
6293 1336 139
BstBI TT'CG_AA
Cuts at: 3784 6572 3784
Size: 2788 4980
BstEII G'GTnAC_C
Cuts at: 1184 2111 1184
Size: 927 6841
BstXI CCAn_nnnn'nTGG
Cuts at: 4770 4770
Size: 7768
BstYI r'GATC_y
Cuts at: 1972 2773 2832 3093 3571 4182 4848 5098 5465
Size: 801 59 261 478 611 666 250 367
Cuts at: 5465 5850 5867 7319 7405 7417 7763 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 801 666 611 478 385 367
346 261 250 86 59 17 12
BstZ17I GTA'TAC
Cuts at: 2643 2643
Size: 7768
Bsu36I CC'TnA_GG
Cuts at: 3218 3218
Size: 7768
BtgI C'CryG_G
Cuts at: 513 1065 2004 3356 3445 4753 513
Size: 552 939 1352 89 1308 3528
Fragments arranged by size:
3528 1352 1308 939 552 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7029
Fragments arranged by size:
7029 683 56
BtsI GCAGTG_nn'
Cuts at: 2850 2935 3504 4961 5284 6078 6098 2850
Size: 85 569 1457 323 794 20 4520
Fragments arranged by size:
4520 1457 794 569 323 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7768
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3222 3257 3292 529
Size: 35 2658 35 35 5005
Fragments arranged by size:
5005 2658 35 35 35
DraI TTT'AAA
Cuts at: 5072 5072
Size: 7768
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3850 6775 809
Size: 507 2534 2925 1802
Fragments arranged by size:
2925 2534 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2143 2932
Size: 54 1425 50 275 18 232 81 789
Cuts at: 2932 4494 5215 6104 7733 8
Size: 1562 721 889 1629 43
Fragments arranged by size:
1629 1562 1425 889 789 721 275 232
81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5215 1537
Size: 275 3403 4090
Fragments arranged by size:
4090 3403 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3897 4623 5698 1353
Size: 2544 726 1075 3423
Fragments arranged by size:
3423 2544 1075 726
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6355 6729 6875 387
Size: 1064 138 4766 374 146 1280
Fragments arranged by size:
4766 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3052 4063 3052
Size: 1011 6757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2321 2705 2910 3035 3038 3167
Size: 393 495 194 384 205 125 3 129
Cuts at: 3167 3482 3512 3635 4109 4208 4532 4637 4706
Size: 315 30 123 474 99 324 105 69
Cuts at: 4706 4809 5037 5831 6428 7215 7483 1239
Size: 103 228 794 597 787 268 1524
Fragments arranged by size:
1524 794 787 597 495 474 393 384
324 315 268 228 205 194 129 125
123 105 103 99 69 30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2564 2564
Size: 7768
EcoO109I rG'GnC_Cy
Cuts at: 2153 2634 2687 3071 2153
Size: 481 53 384 6850
Fragments arranged by size:
6850 481 384 53
EcoRI G'AATT_C
Cuts at: 4039 5077 5455 4039
Size: 1038 378 6352
Fragments arranged by size:
6352 1038 378
FspI TGC'GCA
Cuts at: 6274 6274
Size: 7768
HaeII r_GCGC'y
Cuts at: 1678 3126 6915 1678
Size: 1448 3789 2531
Fragments arranged by size:
3789 2531 1448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 2587 2619 2758
Size: 32 135 32 857 32 126 32 139
Cuts at: 2758 2790 3025 3057 3085 3117 3631 3663 6409
Size: 32 235 32 28 32 514 32 2746
Cuts at: 6409 6441 6483 6515 1373
Size: 32 42 32 2626
Fragments arranged by size:
2746 2626 857 514 235 139 135 126
42 32 32 32 32 32 32 32
32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2863 3666 4959 5112 5358 669
Size: 988 281 925 803 1293 153 246 3079
Fragments arranged by size:
3079 1293 988 925 803 281 246 153
HindIII A'AGCT_T
Cuts at: 748 4382 4688 748
Size: 3634 306 3828
Fragments arranged by size:
3828 3634 306
HpaI GTT'AAC
Cuts at: 3666 5358 3666
Size: 1692 6076
KasI G'GCGC_C
Cuts at: 3122 3122
Size: 7768
KpnI G_GTAC'C
Cuts at: 2560 3437 5093 2560
Size: 877 1656 5235
Fragments arranged by size:
5235 1656 877
MfeI C'AATT_G
Cuts at: 5367 5367
Size: 7768
MluI A'CGCG_T
Cuts at: 7644 7644
Size: 7768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4101 4995 6882 7066 1305
Size: 2796 894 1887 184 2007
Fragments arranged by size:
2796 2007 1887 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 2145 4496 10
Size: 54 1425 343 232 81 2351 3282
Fragments arranged by size:
3282 2351 1425 343 232 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2991 3532 5726 6085 6244 518
Size: 648 1825 541 2194 359 159 2042
Fragments arranged by size:
2194 2042 1825 648 541 359 159
MspA1I CmG'CkG
Cuts at: 1606 2266 2743 2873 3415 3490 4291 4503 4801
Size: 660 477 130 542 75 801 212 298
Cuts at: 4801 5184 5861 7009 7254 1606
Size: 383 677 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 660 542 477 383
298 245 212 130 75
NarI GG'CG_CC
Cuts at: 3123 3123
Size: 7768
NcoI C'CATG_G
Cuts at: 513 1065 2004 3445 4753 513
Size: 552 939 1441 1308 3528
Fragments arranged by size:
3528 1441 1308 939 552
NdeI CA'TA_TG
Cuts at: 387 2020 3529 387
Size: 1633 1509 4626
Fragments arranged by size:
4626 1633 1509
NheI G'CTAG_C
Cuts at: 1052 3755 1052
Size: 2703 5065
NotI GC'GGCC_GC
Cuts at: 5215 5215
Size: 7768
NspI r_CATG'y
Cuts at: 1132 1229 5126 6671 1132
Size: 97 3897 1545 2229
Fragments arranged by size:
3897 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2630 1348
Size: 1282 6486
PciI A'CATG_T
Cuts at: 6667 6667
Size: 7768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4140 1108
Size: 3032 4736
PfoI T'CCnGG_A
Cuts at: 4185 4844 4185
Size: 659 7109
PmeI GTTT'AAAC
Cuts at: 5072 5072
Size: 7768
PmlI CAC'GTG
Cuts at: 3211 3211
Size: 7768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5837 5869 7350 7382 5837
Size: 32 1481 32 6223
Fragments arranged by size:
6223 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2153 2687 2153
Size: 534 7234
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7768
PsiI TTA'TAA
Cuts at: 4211 5338 4211
Size: 1127 6641
PspOMI G'GGCC_C
Cuts at: 2633 3106 2633
Size: 473 7295
PstI C_TGCA'G
Cuts at: 830 4508 5120 830
Size: 3678 612 3478
Fragments arranged by size:
3678 3478 612
PvuI CG_AT'CG
Cuts at: 2001 4321 2001
Size: 2320 5448
PvuII CAG'CTG
Cuts at: 2743 2873 3415 3490 4291 2743
Size: 130 542 75 801 6220
Fragments arranged by size:
6220 801 542 130 75
SacI G_AGCT'C
Cuts at: 3054 4065 3054
Size: 1011 6757
SalI G'TCGA_C
Cuts at: 1936 5110 1936
Size: 3174 4594
SanDI GG'GwC_CC
Cuts at: 2687 2687
Size: 7768
SbfI CC_TGCA'GG
Cuts at: 5120 5120
Size: 7768
ScaI AGT'ACT
Cuts at: 6016 7728 6016
Size: 1712 6056
SexAI A'CCwGG_T
Cuts at: 2552 2552
Size: 7768
SfcI C'TryA_G
Cuts at: 826 1045 3127 4504 4671 5116 6251 6932 7123
Size: 219 2082 1377 167 445 1135 681 191
Cuts at: 7123 826
Size: 1471
Fragments arranged by size:
2082 1471 1377 1135 681 445 219 191
167
SfoI GGC'GCC
Cuts at: 3124 3124
Size: 7768
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7768
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7768
SmaI CCC'GGG
Cuts at: 1872 5095 1872
Size: 3223 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3089 3716 4421 5872 6773 7035
Size: 197 926 1146 627 705 1451 901 262
Cuts at: 7035 820
Size: 1553
Fragments arranged by size:
1553 1451 1146 926 901 705 627 262
197
SnaBI TAC'GTA
Cuts at: 493 5559 493
Size: 5066 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7768
SphI G_CATG'C
Cuts at: 1132 5126 1132
Size: 3994 3774
SspI AAT'ATT
Cuts at: 5 52 5692 5
Size: 47 5640 2081
Fragments arranged by size:
5640 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7768
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 3075 3445 4753 5207 513
Size: 552 939 225 846 370 1308 454 3074
Fragments arranged by size:
3074 1308 939 846 552 454 370 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2828 3407 3806 4008 4481 4776 4987
Size: 1255 1248 579 399 202 473 295 211
Cuts at: 4987 5795 5812 5965 6150 6595 325
Size: 808 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1248 808 579 473 445 399
295 211 202 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4458 4539 4722
Size: 274 80 33 51 1443 2481 81 183
Cuts at: 4722 6014 7726 96
Size: 1292 1712 138
Fragments arranged by size:
2481 1712 1443 1292 274 183 138 81
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7728 7760 7728
Size: 32 7736
Tth111I GACn'n_nGTC
Cuts at: 1316 4679 1316
Size: 3363 4405
XbaI T'CTAG_A
Cuts at: 5104 5104
Size: 7768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2784 3487 2784
Size: 703 7065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7768
XmaI C'CCGG_G
Cuts at: 1870 5093 1870
Size: 3223 4545
XmnI GAAnn'nnTTC
Cuts at: 2766 5897 2766
Size: 3131 4637
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2284 5576 276
Size: 53 83 186 936 750 3292 2468
Fragments arranged by size:
3292 2468 936 750 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacII SapI SfiI
SrfI SwaI