Restriction map for
pFN21AB1891
(Circular) (Six-base) MAPSORT of: pFN21AB1891.seq Check: 4227 from: 1 to: 9094
pFN21AB1891 9094 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6904 278
Size: 53 83 186 936 5368 2468
Fragments arranged by size:
5368 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 3285 4613 6315 6374 6437 1937
Size: 1035 313 1328 1702 59 63 4594
Fragments arranged by size:
4594 1702 1328 1035 313 63 59
Acc65I G'GTAC_C
Cuts at: 6415 6415
Size: 9094
AclI AA'CG_TT
Cuts at: 1563 7221 7594 1563
Size: 5658 373 3063
Fragments arranged by size:
5658 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2545 3069 3945 4218 5496 7157 8541 8809
Size: 913 524 876 273 1278 1661 1384 268
Cuts at: 8809 1632
Size: 1917
Fragments arranged by size:
1917 1661 1384 1278 913 876 524 273
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8897
AflIII A'CryG_T
Cuts at: 1203 2975 3659 3947 7993 8970 1203
Size: 1772 684 288 4046 977 1327
Fragments arranged by size:
4046 1772 1327 977 684 288
AgeI A'CCGG_T
Cuts at: 7925 7925
Size: 9094
AhdI GACnn_n'nnGTC
Cuts at: 7823 7823
Size: 9094
AleI CACnn'nnGTG
Cuts at: 1166 5783 1166
Size: 4617 4477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9094
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2797 2829 5101 5133 2797
Size: 32 2272 32 6758
Fragments arranged by size:
6758 2272 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3242 6837 8409 3242
Size: 3595 1572 3927
Fragments arranged by size:
3927 3595 1572
ApaLI G'TGCA_C
Cuts at: 7151 8307 7151
Size: 1156 7938
ApoI r'AATT_y
Cuts at: 1493 2273 2383 2566 2642 4243 5035 5164 5173
Size: 780 110 183 76 1601 792 129 9
Cuts at: 5173 5320 5506 6138 6277 6403 6629 6781 8890
Size: 147 186 632 139 126 226 152 2109
Cuts at: 8890 1493
Size: 1697
Fragments arranged by size:
2109 1697 1601 792 780 632 226 186
183 152 147 139 129 126 110 76
9
AseI AT'TA_AT
Cuts at: 160 1032 7648 160
Size: 872 6616 1606
Fragments arranged by size:
6616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9094
AvaI C'yCGr_G
Cuts at: 1870 1943 3488 6419 1870
Size: 73 1545 2931 4545
Fragments arranged by size:
4545 2931 1545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5802 5835 5802
Size: 33 9061
BamHI G'GATC_C
Cuts at: 6424 6791 6424
Size: 367 8727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6415 7870 618
Size: 325 217 627 4628 1455 1842
Fragments arranged by size:
4628 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3182 3830 3893 6413 1391
Size: 1791 648 63 2520 4072
Fragments arranged by size:
4072 2520 1791 648 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2182 4272 6125 928
Size: 971 162 121 2090 1853 3897
Fragments arranged by size:
3897 2090 1853 971 162 121
BbvCI CC'TCA_GC
Cuts at: 5757 5757
Size: 9094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6096 6130 7285 7319 1903
Size: 34 4159 34 1155 34 3678
Fragments arranged by size:
4159 3678 1155 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2454 6986 8196 2454
Size: 4532 1210 3352
Fragments arranged by size:
4532 3352 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9094
BfrBI ATG'CAT
Cuts at: 2867 2867
Size: 9094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7705 136
Size: 107 122 71 631 605 6033 1525
Fragments arranged by size:
6033 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2425 3445 4082 4376 4634 4682 4811 5420 9089
Size: 1020 637 294 258 48 129 609 3669
Cuts at: 9089 2425
Size: 2430
Fragments arranged by size:
3669 2430 1020 637 609 294 258 129
48
BlpI GC'TnA_GC
Cuts at: 6511 6511
Size: 9094
Bme1580I G_kGCm'C
Cuts at: 1790 5790 7155 8311 1790
Size: 4000 1365 1156 2573
Fragments arranged by size:
4000 2573 1365 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4441 4482 6825
Size: 416 53 83 399 24 3024 41 2343
Cuts at: 6825 7783 442
Size: 958 1753
Fragments arranged by size:
3024 2343 1753 958 416 399 83 53
41 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4605 5807 4605
Size: 1202 7892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2625 7754 1239
Size: 1386 5129 2579
Fragments arranged by size:
5129 2579 1386
Bpu10I CC'TnA_GC
Cuts at: 5757 5757
Size: 9094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2865 3426 4816 7219 8084 8382 2865
Size: 561 1390 2403 865 298 3577
Fragments arranged by size:
3577 2403 1390 865 561 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2374 4605 7757 882
Size: 617 875 2231 3152 2219
Fragments arranged by size:
3152 2231 2219 875 617
BsaAI yAC'GTr
Cuts at: 493 1204 2978 6885 493
Size: 711 1774 3907 2702
Fragments arranged by size:
3907 2702 1774 711
BsaBI GATnn'nnATC
Cuts at: 3421 4639 5431 5726 9088 3421
Size: 1218 792 295 3362 3427
Fragments arranged by size:
3427 3362 1218 792 295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6901 7283 7918 275
Size: 53 83 186 936 5368 382 635 1451
Fragments arranged by size:
5368 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7527 7925 8199 8346 8757 7527
Size: 398 274 147 411 7864
Fragments arranged by size:
7864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8869
Fragments arranged by size:
8869 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3827 4783 5558 1186
Size: 2641 956 775 4722
Fragments arranged by size:
4722 2641 956 775
BseYI C'CCAG_C
Cuts at: 5445 8297 8793 5445
Size: 2852 496 5746
Fragments arranged by size:
5746 2852 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3329 1163
Size: 573 1593 6928
Fragments arranged by size:
6928 1593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6544 7305 8333 1540
Size: 275 186 4543 761 1028 2301
Fragments arranged by size:
4543 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3182 3273 3442 7155 7240 8311 3182
Size: 91 169 3713 85 1071 3965
Fragments arranged by size:
3965 3713 1071 169 91 85
BsmI GAATG_Cn'
Cuts at: 5668 6605 6698 5668
Size: 937 93 8064
Fragments arranged by size:
8064 937 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3182 3273 3442 3830 3893 5790 6413
Size: 399 1392 91 169 388 63 1897 623
Cuts at: 6413 7155 7240 8311 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1897 1392 1071 742 623 399 388
169 91 85 63
BspEI T'CCGG_A
Cuts at: 8757 8757
Size: 9094
BspHI T'CATG_A
Cuts at: 2377 6983 8713 2377
Size: 4606 1730 2758
Fragments arranged by size:
4606 2758 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2953 5788 6449 844
Size: 2109 2835 661 3489
Fragments arranged by size:
3489 2835 2109 661
BsrBI CCG'CTC
Cuts at: 1394 1823 6981 1394
Size: 429 5158 3507
Fragments arranged by size:
5158 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2955 3361 5877 7589 7763 65
Size: 1183 1707 406 2516 1712 174 1396
Fragments arranged by size:
2516 1712 1707 1396 1183 406 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7738 7925 1700
Size: 110 5928 187 2869
Fragments arranged by size:
5928 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 2636 3629 5476 96
Size: 2010 530 993 1847 3714
Fragments arranged by size:
3714 2010 1847 993 530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9094
BssSI C'ACGA_G
Cuts at: 4938 7154 8166 4938
Size: 2216 1012 5866
Fragments arranged by size:
5866 2216 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9094
BstBI TT'CG_AA
Cuts at: 2835 7898 2835
Size: 5063 4031
BstEII G'GTnAC_C
Cuts at: 1184 4407 1184
Size: 3223 5871
BstXI CCAn_nnnn'nTGG
Cuts at: 3593 3593
Size: 9094
BstYI r'GATC_y
Cuts at: 1972 2425 3014 3416 3445 4082 4376 4634 4682
Size: 453 589 402 29 637 294 258 48
Cuts at: 4682 4811 5420 6424 6791 7176 7193 8645 8731
Size: 129 609 1004 367 385 17 1452 86
Cuts at: 8731 8743 9089 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1004 637 609 589 453 402
385 367 346 294 258 129 86 48
29 17 12
BstZ17I GTA'TAC
Cuts at: 4614 4614
Size: 9094
BtgI C'CryG_G
Cuts at: 513 1065 5215 5236 513
Size: 552 4150 21 4371
Fragments arranged by size:
4371 4150 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8355
Fragments arranged by size:
8355 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 4186 6099 6610 7404 7424 2271
Size: 1915 1913 511 794 20 3941
Fragments arranged by size:
3941 1915 1913 794 511 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9059
DraI TTT'AAA
Cuts at: 2272 6398 2272
Size: 4126 4968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8101 809
Size: 507 6785 1802
Fragments arranged by size:
6785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6171 6541 7430
Size: 54 1425 50 275 18 4341 370 889
Cuts at: 7430 9059 8
Size: 1629 43
Fragments arranged by size:
4341 1629 1425 889 370 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6541 1537
Size: 275 4729 4090
Fragments arranged by size:
4729 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 3088 3133 4459 4651 7024 1353
Size: 1325 410 45 1326 192 2373 3423
Fragments arranged by size:
3423 2373 1326 1325 410 192 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7681 8055 8201 387
Size: 1064 138 6092 374 146 1280
Fragments arranged by size:
6092 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3180 3180
Size: 9094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2545 2625 3069 3945 4218 5496 7157
Size: 393 913 80 444 876 273 1278 1661
Cuts at: 7157 7754 8541 8809 1239
Size: 597 787 268 1524
Fragments arranged by size:
1661 1524 1278 913 876 787 597 444
393 273 268 80
EcoNI CCTnn'n_nnAGG
Cuts at: 5795 5795
Size: 9094
EcoO109I rG'GnC_Cy
Cuts at: 4728 4824 4728
Size: 96 8998
EcoRI G'AATT_C
Cuts at: 5320 6403 6781 5320
Size: 1083 378 7633
Fragments arranged by size:
7633 1083 378
EcoRV GAT'ATC
Cuts at: 3591 6202 3591
Size: 2611 6483
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4680 4680
Size: 9094
FspI TGC'GCA
Cuts at: 7600 7600
Size: 9094
HaeII r_GCGC'y
Cuts at: 1678 8241 1678
Size: 6563 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2536 2568 3575 3607 3863
Size: 32 135 32 964 32 1007 32 256
Cuts at: 3863 3895 4060 4092 4874 4906 5537 5569 7735
Size: 32 165 32 782 32 631 32 2166
Cuts at: 7735 7767 7809 7841 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2166 1007 964 782 631 256 165
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5872 6438 6684 669
Size: 988 281 3934 566 246 3079
Fragments arranged by size:
3934 3079 988 566 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9094
HpaI GTT'AAC
Cuts at: 6684 6684
Size: 9094
KpnI G_GTAC'C
Cuts at: 6419 6419
Size: 9094
MfeI C'AATT_G
Cuts at: 3902 5068 5903 6693 3902
Size: 1166 835 790 6303
Fragments arranged by size:
6303 1166 835 790
MluI A'CGCG_T
Cuts at: 8970 8970
Size: 9094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2840 4907 5755 5993 8208 8392 1305
Size: 1535 2067 848 238 2215 184 2007
Fragments arranged by size:
2215 2067 2007 1535 848 238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6173 10
Size: 54 1425 343 4341 2931
Fragments arranged by size:
4341 2931 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4131 5019 5055 5783 7052 7411 7570
Size: 648 2965 888 36 728 1269 359 159
Cuts at: 7570 518
Size: 2042
Fragments arranged by size:
2965 2042 1269 888 728 648 359 159
36
MspA1I CmG'CkG
Cuts at: 1606 2320 3089 5445 6510 7187 8335 8580 1606
Size: 714 769 2356 1065 677 1148 245 2120
Fragments arranged by size:
2356 2120 1148 1065 769 714 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8542
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9094
NotI GC'GGCC_GC
Cuts at: 6541 6541
Size: 9094
NsiI A_TGCA'T
Cuts at: 2869 2869
Size: 9094
NspI r_CATG'y
Cuts at: 1132 1229 3663 3917 3951 6452 7997 1132
Size: 97 2434 254 34 2501 1545 2229
Fragments arranged by size:
2501 2434 2229 1545 254 97 34
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9094
PciI A'CATG_T
Cuts at: 3659 3947 7993 3659
Size: 288 4046 4760
Fragments arranged by size:
4760 4046 288
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4797 5525 6014 1108
Size: 3689 728 489 4188
Fragments arranged by size:
4188 3689 728 489
PmeI GTTT'AAAC
Cuts at: 6398 6398
Size: 9094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7163 7195 8676 8708 7163
Size: 32 1481 32 7549
Fragments arranged by size:
7549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4728 4824 4728
Size: 96 8998
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9094
PsiI TTA'TAA
Cuts at: 6664 6664
Size: 9094
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3618 3650 5905 5937 3618
Size: 32 2255 32 6775
Fragments arranged by size:
6775 2255 32 32
PstI C_TGCA'G
Cuts at: 830 6446 830
Size: 5616 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9094
PvuII CAG'CTG
Cuts at: 2320 3089 5445 2320
Size: 769 2356 5969
Fragments arranged by size:
5969 2356 769
SacI G_AGCT'C
Cuts at: 3182 3182
Size: 9094
SalI G'TCGA_C
Cuts at: 1936 6436 1936
Size: 4500 4594
SbfI CC_TGCA'GG
Cuts at: 6446 6446
Size: 9094
ScaI AGT'ACT
Cuts at: 7342 9054 7342
Size: 1712 7382
SfcI C'TryA_G
Cuts at: 826 1045 2623 3183 4086 6442 7577 8258 8449
Size: 219 1578 560 903 2356 1135 681 191
Cuts at: 8449 826
Size: 1471
Fragments arranged by size:
2356 1578 1471 1135 903 681 560 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9094
SmaI CCC'GGG
Cuts at: 1872 6421 1872
Size: 4549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2880 3441 3488 4831 7198 8099
Size: 197 926 937 561 47 1343 2367 901
Cuts at: 8099 8361 820
Size: 262 1553
Fragments arranged by size:
2367 1553 1343 937 926 901 561 262
197 47
SnaBI TAC'GTA
Cuts at: 493 6885 493
Size: 6392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9094
SphI G_CATG'C
Cuts at: 1132 6452 1132
Size: 5320 3774
SspI AAT'ATT
Cuts at: 5 52 3547 3982 6329 7018 5
Size: 47 3495 435 2347 689 2081
Fragments arranged by size:
3495 2347 2081 689 435 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9094
StyI C'CwwG_G
Cuts at: 513 1065 6207 6533 513
Size: 552 5142 326 3074
Fragments arranged by size:
5142 3074 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3027 4085 4426 4587 5693 7121 7138
Size: 1255 1447 1058 341 161 1106 1428 17
Cuts at: 7138 7291 7476 7921 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1447 1428 1255 1106 1058 445 341
185 161 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2546 2636
Size: 274 80 33 51 1443 129 440 90
Cuts at: 2636 3302 3629 5476 5948 7340 9052 96
Size: 666 327 1847 472 1392 1712 138
Fragments arranged by size:
1847 1712 1443 1392 666 472 440 327
274 138 129 90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9054 9086 9054
Size: 32 9062
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9094
XbaI T'CTAG_A
Cuts at: 4379 4667 5017 6430 4379
Size: 288 350 1413 7043
Fragments arranged by size:
7043 1413 350 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4401 5526 5597 4401
Size: 1125 71 7898
Fragments arranged by size:
7898 1125 71
XhoI C'TCGA_G
Cuts at: 1943 3488 1943
Size: 1545 7549
XmaI C'CCGG_G
Cuts at: 1870 6419 1870
Size: 4549 4545
XmnI GAAnn'nnTTC
Cuts at: 2775 3546 4116 5509 5687 7223 2775
Size: 771 570 1393 178 1536 4646
Fragments arranged by size:
4646 1536 1393 771 570 178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6902 276
Size: 53 83 186 936 5368 2468
Fragments arranged by size:
5368 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BsiWI BsmBI
Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI