Restriction map for
pFN21AB1755
(Circular) (Six-base) MAPSORT of: pFN21AB1755.seq Check: 9470 from: 1 to: 9100
pFN21AB1755 9100 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3045 3045
Size: 9100
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2189 2838 3884 4107
Size: 53 83 186 936 653 649 1046 223
Cuts at: 4107 6910 278
Size: 2803 2468
Fragments arranged by size:
2803 2468 1046 936 653 649 223 186
83 53
AccI GT'mk_AC
Cuts at: 1937 6443 1937
Size: 4506 4594
Acc65I G'GTAC_C
Cuts at: 2082 2268 2952 6421 2082
Size: 186 684 3469 4761
Fragments arranged by size:
4761 3469 684 186
AclI AA'CG_TT
Cuts at: 1563 7227 7600 1563
Size: 5664 373 3063
Fragments arranged by size:
5664 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2851 2945 3013 4540 4642 5735 5738 7163
Size: 1219 94 68 1527 102 1093 3 1425
Cuts at: 7163 8547 8815 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1527 1425 1384 1219 1093 268 102
94 68 3
AfeI AGC'GCT
Cuts at: 2312 2312
Size: 9100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8903
AflIII A'CryG_T
Cuts at: 1203 4482 4704 4815 7999 8976 1203
Size: 3279 222 111 3184 977 1327
Fragments arranged by size:
3279 3184 1327 977 222 111
AgeI A'CCGG_T
Cuts at: 3310 3548 7931 3310
Size: 238 4383 4479
Fragments arranged by size:
4479 4383 238
AhdI GACnn_n'nnGTC
Cuts at: 4972 7829 4972
Size: 2857 6243
AleI CACnn'nnGTG
Cuts at: 1166 3551 1166
Size: 2385 6715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9100
AlwNI CAG_nnn'CTG
Cuts at: 2214 2278 6843 8415 2214
Size: 64 4565 1572 2899
Fragments arranged by size:
4565 2899 1572 64
ApaI G_GGCC'C
Cuts at: 3151 3151
Size: 9100
ApaLI G'TGCA_C
Cuts at: 2899 3156 3554 4016 7157 8313 2899
Size: 257 398 462 3141 1156 3686
Fragments arranged by size:
3686 3141 1156 462 398 257
ApoI r'AATT_y
Cuts at: 1493 4087 4208 5372 5990 6409 6635 6787 8896
Size: 2594 121 1164 618 419 226 152 2109
Cuts at: 8896 1493
Size: 1697
Fragments arranged by size:
2594 2109 1697 1164 618 419 226 152
121
AseI AT'TA_AT
Cuts at: 160 1032 5157 7654 160
Size: 872 4125 2497 1606
Fragments arranged by size:
4125 2497 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9100
AvaI C'yCGr_G
Cuts at: 1870 1943 3081 3706 5006 6241 6425 1870
Size: 73 1138 625 1300 1235 184 4545
Fragments arranged by size:
4545 1300 1235 1138 625 184 73
BamHI G'GATC_C
Cuts at: 6430 6797 6430
Size: 367 8733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 2268 2952 4739 6421
Size: 325 217 627 295 186 684 1787 1682
Cuts at: 6421 7876 618
Size: 1455 1842
Fragments arranged by size:
1842 1787 1682 1455 684 627 325 295
217 186
BanII G_rGCy'C
Cuts at: 1391 2464 3151 3242 3978 4392 4443 5007 5559
Size: 1073 687 91 736 414 51 564 552
Cuts at: 5559 6419 1391
Size: 860 4072
Fragments arranged by size:
4072 1073 860 736 687 564 552 414
91 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 4648 5298 6089 928
Size: 971 255 2494 650 791 3939
Fragments arranged by size:
3939 2494 971 791 650 255
BbvCI CC'TCA_GC
Cuts at: 2318 3590 6385 2318
Size: 1272 2795 5033
Fragments arranged by size:
5033 2795 1272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2779 2813 5433 5467 6025 6059 7291
Size: 34 842 34 2620 34 558 34 1232
Cuts at: 7291 7325 1903
Size: 34 3678
Fragments arranged by size:
3678 2620 1232 842 558 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 6992 8202 3992
Size: 3000 1210 4890
Fragments arranged by size:
4890 3000 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9100
BfrBI ATG'CAT
Cuts at: 5330 5330
Size: 9100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6389 7711 136
Size: 107 122 71 631 605 4717 1322 1525
Fragments arranged by size:
4717 1525 1322 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5814 9095 5814
Size: 3281 5819
BlpI GC'TnA_GC
Cuts at: 5118 6164 6517 5118
Size: 1046 353 7701
Fragments arranged by size:
7701 1046 353
Bme1580I G_kGCm'C
Cuts at: 1790 2903 3151 3160 3558 4020 7161 8317 1790
Size: 1113 248 9 398 462 3141 1156 2573
Fragments arranged by size:
3141 2573 1156 1113 462 398 248 9
BmgBI CAC'GTC
Cuts at: 1318 2965 4485 4707 6061 6295 1318
Size: 1647 1520 222 1354 234 4123
Fragments arranged by size:
4123 1647 1520 1354 234 222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4395 5425 6831
Size: 416 53 83 399 24 2978 1030 1406
Cuts at: 6831 7789 442
Size: 958 1753
Fragments arranged by size:
2978 1753 1406 1030 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6193 6193
Size: 9100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2161 2666 3394 4593 4963 5096 5558 7760
Size: 922 505 728 1199 370 133 462 2202
Cuts at: 7760 1239
Size: 2579
Fragments arranged by size:
2579 2202 1199 922 728 505 462 370
133
Bpu10I CC'TnA_GC
Cuts at: 2318 3590 3782 3909 6385 2318
Size: 1272 192 127 2476 5033
Fragments arranged by size:
5033 2476 1272 192 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 2879 5480 7225 8090 8388 2167
Size: 712 2601 1745 865 298 2879
Fragments arranged by size:
2879 2601 1745 865 712 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3421 4630 4726 7763 882
Size: 617 1922 1209 96 3037 2219
Fragments arranged by size:
3037 2219 1922 1209 617 96
BsaAI yAC'GTr
Cuts at: 493 1204 3940 6891 493
Size: 711 2736 2951 2702
Fragments arranged by size:
2951 2736 2702 711
BsaBI GATnn'nnATC
Cuts at: 9094 9094
Size: 9100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2186 2835 2915 3090
Size: 53 83 186 936 653 649 80 175
Cuts at: 3090 3183 3525 3881 4104 6907 7289 7924 275
Size: 93 342 356 223 2803 382 635 1451
Fragments arranged by size:
2803 1451 936 653 649 635 382 356
342 223 186 175 93 83 80 53
BsaWI w'CCGG_w
Cuts at: 3310 3548 4918 5960 7533 7931 8205 8352 8763
Size: 238 1370 1042 1573 398 274 147 411
Cuts at: 8763 3310
Size: 3647
Fragments arranged by size:
3647 1573 1370 1042 411 398 274 238
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 3788 3818 4163
Size: 30 165 30 1092 30 1263 30 345
Cuts at: 4163 4193 1178
Size: 30 6085
Fragments arranged by size:
6085 1263 1092 345 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2354 3932 4151 4577 4862 5549 1186
Size: 1075 93 1578 219 426 285 687 4737
Fragments arranged by size:
4737 1578 1075 687 426 285 219 93
BseYI C'CCAG_C
Cuts at: 3594 3928 4295 6348 8303 8799 3594
Size: 334 367 2053 1955 496 3895
Fragments arranged by size:
3895 2053 1955 496 367 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2579 2954 3023 3314 3417 3914 4136
Size: 573 843 375 69 291 103 497 222
Cuts at: 4136 5294 5713 6085 1163
Size: 1158 419 372 4178
Fragments arranged by size:
4178 1158 843 573 497 419 375 372
291 222 103 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2159 6550 7311 8339 1540
Size: 275 186 158 4391 761 1028 2301
Fragments arranged by size:
4391 2301 1028 761 275 186 158
BsiHKAI G_wGCw'C
Cuts at: 2234 2903 3160 3558 4020 4410 5007 5124 5559
Size: 669 257 398 462 390 597 117 435
Cuts at: 5559 6174 7161 7246 8317 2234
Size: 615 987 85 1071 3017
Fragments arranged by size:
3017 1071 987 669 615 597 462 435
398 390 257 117 85
BsmI GAATG_Cn'
Cuts at: 5330 6611 6704 5330
Size: 1281 93 7726
Fragments arranged by size:
7726 1281 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2751 2876 4824 2751
Size: 125 1948 7027
Fragments arranged by size:
7027 1948 125
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2464 2903 3151 3160 3242 3558
Size: 399 444 230 439 248 9 82 316
Cuts at: 3558 3978 4020 4392 4410 4443 5007 5124 5559
Size: 420 42 372 18 33 564 117 435
Cuts at: 5559 6174 6419 7161 7246 8317 1391
Size: 615 245 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 564 444 439 435
420 399 372 316 248 245 230 117
85 82 42 33 18 9
BspEI T'CCGG_A
Cuts at: 4918 5960 8763 4918
Size: 1042 2803 5255
Fragments arranged by size:
5255 2803 1042
BspHI T'CATG_A
Cuts at: 5281 5760 6989 8719 5281
Size: 479 1229 1730 5662
Fragments arranged by size:
5662 1730 1229 479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2256 3045 3933 4641 6027 6107 6455
Size: 1250 162 789 888 708 1386 80 348
Cuts at: 6455 844
Size: 3489
Fragments arranged by size:
3489 1386 1250 888 789 708 348 162
80
BsrBI CCG'CTC
Cuts at: 1394 1823 2449 3628 3663 6987 1394
Size: 429 626 1179 35 3324 3507
Fragments arranged by size:
3507 3324 1179 626 429 35
BsrDI GCAATG_nn'
Cuts at: 65 1248 3307 5035 5688 7595 7769 65
Size: 1183 2059 1728 653 1907 174 1396
Fragments arranged by size:
2059 1907 1728 1396 1183 653 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3310 3548 3771 4376 7744 7931 1700
Size: 110 1500 238 223 605 3368 187 2869
Fragments arranged by size:
3368 2869 1500 605 238 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 3060 6309 96
Size: 2964 3249 2887
Fragments arranged by size:
3249 2964 2887
BssHII G'CGCG_C
Cuts at: 1925 2941 1925
Size: 1016 8084
BssSI C'ACGA_G
Cuts at: 4277 7160 8172 4277
Size: 2883 1012 5205
Fragments arranged by size:
5205 2883 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4676 794
Size: 3882 5218
BstBI TT'CG_AA
Cuts at: 7904 7904
Size: 9100
BstEII G'GTnAC_C
Cuts at: 1184 2860 6064 1184
Size: 1676 3204 4220
Fragments arranged by size:
4220 3204 1676
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 4148 2554
Size: 1594 7506
BstYI r'GATC_y
Cuts at: 1972 2469 3701 4396 5814 6327 6430 6797 7182
Size: 497 1232 695 1418 513 103 367 385
Cuts at: 7182 7199 8651 8737 8749 9095 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1418 1232 695 513 497 385
367 346 103 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2553 2707 3162 3613 3651 4029 4884
Size: 552 1488 154 455 451 38 378 855
Cuts at: 4884 5587 513
Size: 703 4026
Fragments arranged by size:
4026 1488 855 703 552 455 451 378
154 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2973 487
Size: 683 56 1747 6614
Fragments arranged by size:
6614 1747 683 56
BtsI GCAGTG_nn'
Cuts at: 2567 2673 2697 3179 3302 3387 3902 5525 6616
Size: 106 24 482 123 85 515 1623 1091
Cuts at: 6616 7410 7430 2567
Size: 794 20 4237
Fragments arranged by size:
4237 1623 1091 794 515 482 123 106
85 24 20
ClaI AT'CG_AT
Cuts at: 1554 2810 5239 1554
Size: 1256 2429 5415
Fragments arranged by size:
5415 2429 1256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4668 4703 529
Size: 35 4104 35 4926
Fragments arranged by size:
4926 4104 35 35
DraI TTT'AAA
Cuts at: 4620 4873 6404 4620
Size: 253 1531 7316
Fragments arranged by size:
7316 1531 253
DraIII CAC_nnn'GTG
Cuts at: 4833 4833
Size: 9100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3137 4816 5168 8107 809
Size: 507 1821 1679 352 2939 1802
Fragments arranged by size:
2939 1821 1802 1679 507 352
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2694 3954
Size: 54 1425 50 275 18 453 411 1260
Cuts at: 3954 4174 4374 6547 7436 9065 8
Size: 220 200 2173 889 1629 43
Fragments arranged by size:
2173 1629 1425 1260 889 453 411 275
220 200 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6547 1537
Size: 275 4735 4090
Fragments arranged by size:
4735 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2439 4995 5343 5847 7030 1353
Size: 677 409 2556 348 504 1183 3423
Fragments arranged by size:
3423 2556 1183 677 504 409 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7687 8061 8207 387
Size: 1064 138 6098 374 146 1280
Fragments arranged by size:
6098 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5005 5557 5005
Size: 552 8548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 2666 2851 2945 3013 3394 4540
Size: 393 529 505 185 94 68 381 1146
Cuts at: 4540 4593 4642 4963 5096 5558 5735 5738 7163
Size: 53 49 321 133 462 177 3 1425
Cuts at: 7163 7760 8547 8815 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1425 1146 787 597 529 505 462
393 381 321 268 185 177 133 94
68 53 49 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2869 3041 2869
Size: 172 8928
EcoO109I rG'GnC_Cy
Cuts at: 3055 3147 3148 3539 3622 3922 4201 4507 5262
Size: 92 1 391 83 300 279 306 755
Cuts at: 5262 5390 3055
Size: 128 6765
Fragments arranged by size:
6765 755 391 306 300 279 128 92
83 1
EcoRI G'AATT_C
Cuts at: 5990 6409 6787 5990
Size: 419 378 8303
Fragments arranged by size:
8303 419 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 4924 5322 5462 5623 3016
Size: 1908 398 140 161 6493
Fragments arranged by size:
6493 1908 398 161 140
FspI TGC'GCA
Cuts at: 3071 7606 3071
Size: 4535 4565
HaeII r_GCGC'y
Cuts at: 1678 2314 3108 8247 1678
Size: 636 794 5139 2531
Fragments arranged by size:
5139 2531 794 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2187 2219 2495 2527 2875
Size: 32 135 32 615 32 276 32 348
Cuts at: 2875 2907 3788 3820 4163 4195 4566 4598 4958
Size: 32 881 32 343 32 371 32 360
Cuts at: 4958 4990 5111 5143 6076 6108 7741 7773 7815
Size: 32 121 32 933 32 1633 32 42
Cuts at: 7815 7847 1373
Size: 32 2626
Fragments arranged by size:
2626 1633 933 881 615 371 360 348
343 276 135 121 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3682 4369 6444 6690 669
Size: 988 281 1744 687 2075 246 3079
Fragments arranged by size:
3079 2075 1744 988 687 281 246
HindIII A'AGCT_T
Cuts at: 748 5967 748
Size: 5219 3881
HpaI GTT'AAC
Cuts at: 6690 6690
Size: 9100
KpnI G_GTAC'C
Cuts at: 2086 2272 2956 6425 2086
Size: 186 684 3469 4761
Fragments arranged by size:
4761 3469 684 186
MfeI C'AATT_G
Cuts at: 6699 6699
Size: 9100
MluI A'CGCG_T
Cuts at: 4815 8976 4815
Size: 4161 4939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4689 8214 8398 1305
Size: 3384 3525 184 2007
Fragments arranged by size:
3525 3384 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 10
Size: 54 1425 343 864 6414
Fragments arranged by size:
6414 1425 864 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 2909 3551 4112 4146 5418 5805
Size: 648 1386 357 642 561 34 1272 387
Cuts at: 5805 7058 7417 7576 518
Size: 1253 359 159 2042
Fragments arranged by size:
2042 1386 1272 1253 648 642 561 387
359 357 159 34
MspA1I CmG'CkG
Cuts at: 1606 2155 3136 3594 3669 3695 6389 6516 7193
Size: 549 981 458 75 26 2694 127 677
Cuts at: 7193 8341 8586 1606
Size: 1148 245 2120
Fragments arranged by size:
2694 2120 1148 981 677 549 458 245
127 75 26
NaeI GCC'GGC
Cuts at: 4378 4378
Size: 9100
NcoI C'CATG_G
Cuts at: 513 1065 3162 513
Size: 552 2097 6451
Fragments arranged by size:
6451 2097 552
NdeI CA'TA_TG
Cuts at: 387 5832 387
Size: 5445 3655
NgoMIV G'CCGG_C
Cuts at: 4376 4376
Size: 9100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9100
NotI GC'GGCC_GC
Cuts at: 6547 6547
Size: 9100
NsiI A_TGCA'T
Cuts at: 5332 5332
Size: 9100
NspI r_CATG'y
Cuts at: 1132 1229 6458 8003 1132
Size: 97 5229 1545 2229
Fragments arranged by size:
5229 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2191 1348
Size: 843 8257
PciI A'CATG_T
Cuts at: 7999 7999
Size: 9100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2278 2845 3969 1108
Size: 1170 567 1124 6239
Fragments arranged by size:
6239 1170 1124 567
PfoI T'CCnGG_A
Cuts at: 6330 6330
Size: 9100
PmeI GTTT'AAAC
Cuts at: 6404 6404
Size: 9100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7169 7201 8682 8714 7169
Size: 32 1481 32 7555
Fragments arranged by size:
7555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3622 3922 4201 5262 3622
Size: 300 279 1061 7460
Fragments arranged by size:
7460 1061 300 279
PshAI GACnn'nnGTC
Cuts at: 1613 2716 3833 4652 1613
Size: 1103 1117 819 6061
Fragments arranged by size:
6061 1117 1103 819
PsiI TTA'TAA
Cuts at: 6670 6670
Size: 9100
PspOMI G'GGCC_C
Cuts at: 3147 3147
Size: 9100
PspXI vC'TCGA_Gb
Cuts at: 5006 5006
Size: 9100
PstI C_TGCA'G
Cuts at: 830 2210 4638 5871 6452 830
Size: 1380 2428 1233 581 3478
Fragments arranged by size:
3478 2428 1380 1233 581
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9100
PvuII CAG'CTG
Cuts at: 3594 3669 3695 3594
Size: 75 26 8999
Fragments arranged by size:
8999 75 26
SacI G_AGCT'C
Cuts at: 5007 5559 5007
Size: 552 8548
SalI G'TCGA_C
Cuts at: 1936 6442 1936
Size: 4506 4594
SapI GCTCTTCn'nnn_
Cuts at: 2439 4995 2439
Size: 2556 6544
SbfI CC_TGCA'GG
Cuts at: 6452 6452
Size: 9100
ScaI AGT'ACT
Cuts at: 5408 7348 9060 5408
Size: 1940 1712 5448
Fragments arranged by size:
5448 1940 1712
SexAI A'CCwGG_T
Cuts at: 4829 4829
Size: 9100
SfcI C'TryA_G
Cuts at: 826 1045 2206 4634 5867 6448 7583 8264 8455
Size: 219 1161 2428 1233 581 1135 681 191
Cuts at: 8455 826
Size: 1471
Fragments arranged by size:
2428 1471 1233 1161 1135 681 581 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9100
SgrAI Cr'CCGG_yG
Cuts at: 1700 3310 3548 1700
Size: 1610 238 7252
Fragments arranged by size:
7252 1610 238
SmaI CCC'GGG
Cuts at: 1872 6427 1872
Size: 4555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2146 2894 5006 5495 7204 8105
Size: 197 926 203 748 2112 489 1709 901
Cuts at: 8105 8367 820
Size: 262 1553
Fragments arranged by size:
2112 1709 1553 926 901 748 489 262
203 197
SnaBI TAC'GTA
Cuts at: 493 6891 493
Size: 6398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9100
SphI G_CATG'C
Cuts at: 1132 6458 1132
Size: 5326 3774
SspI AAT'ATT
Cuts at: 5 52 2071 7024 5
Size: 47 2019 4953 2081
Fragments arranged by size:
4953 2081 2019 47
StuI AGG'CCT
Cuts at: 1517 3017 4424 1517
Size: 1500 1407 6193
Fragments arranged by size:
6193 1500 1407
StyI C'CwwG_G
Cuts at: 513 1065 3162 3444 4488 4743 6539 513
Size: 552 2097 282 1044 255 1796 3074
Fragments arranged by size:
3074 2097 1796 1044 552 282 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 3175 3279 3280 3619 3918 4309
Size: 1255 558 1037 104 1 339 299 391
Cuts at: 4309 7127 7144 7297 7482 7927 325
Size: 2818 17 153 185 445 1498
Fragments arranged by size:
2818 1498 1255 1037 558 445 391 339
299 185 153 104 17 1
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2610 3060 3633
Size: 274 80 33 51 1443 633 450 573
Cuts at: 3633 4041 4515 4905 5406 6249 6309 7346 9058
Size: 408 474 390 501 843 60 1037 1712
Cuts at: 9058 96
Size: 138
Fragments arranged by size:
1712 1443 1037 843 633 573 501 474
450 408 390 274 138 80 60 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3158 3190 4116 4148 4533 4565 9060 9092 3158
Size: 32 926 32 385 32 4495 32 3166
Fragments arranged by size:
4495 3166 926 385 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3197 1316
Size: 1881 7219
XbaI T'CTAG_A
Cuts at: 6436 6436
Size: 9100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2731 3883 4987 2731
Size: 1152 1104 6844
Fragments arranged by size:
6844 1152 1104
XhoI C'TCGA_G
Cuts at: 1943 5006 1943
Size: 3063 6037
XmaI C'CCGG_G
Cuts at: 1870 6425 1870
Size: 4555 4545
XmnI GAAnn'nnTTC
Cuts at: 2996 7229 2996
Size: 4233 4867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2187 2836 3882 4105
Size: 53 83 186 936 653 649 1046 223
Cuts at: 4105 6908 276
Size: 2803 2468
Fragments arranged by size:
2803 2468 1046 936 653 649 223 186
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI