Restriction map for
pFN21AB1630
(Circular) (Six-base) MAPSORT of: pFN21AB1630.seq Check: 9323 from: 1 to: 7861
pFN21AB1630 7861 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5671 278
Size: 53 83 186 936 4135 2468
Fragments arranged by size:
4135 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2720 3275 5204 1937
Size: 783 555 1929 4594
Fragments arranged by size:
4594 1929 783 555
Acc65I G'GTAC_C
Cuts at: 2758 4402 5182 2758
Size: 1644 780 5437
Fragments arranged by size:
5437 1644 780
AclI AA'CG_TT
Cuts at: 1563 5988 6361 1563
Size: 4425 373 3063
Fragments arranged by size:
4425 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3008 3502 3749 4869 5924 7308 7576 1632
Size: 1376 494 247 1120 1055 1384 268 1917
Fragments arranged by size:
1917 1384 1376 1120 1055 494 268 247
AfeI AGC'GCT
Cuts at: 2155 2155
Size: 7861
AflII C'TTAA_G
Cuts at: 820 1017 4970 820
Size: 197 3953 3711
Fragments arranged by size:
3953 3711 197
AflIII A'CryG_T
Cuts at: 1203 3680 3982 6760 7737 1203
Size: 2477 302 2778 977 1327
Fragments arranged by size:
2778 2477 1327 977 302
AgeI A'CCGG_T
Cuts at: 6692 6692
Size: 7861
AhdI GACnn_n'nnGTC
Cuts at: 6590 6590
Size: 7861
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2154 1184
Size: 970 6891
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 4313 4345 2396
Size: 32 1885 32 5912
Fragments arranged by size:
5912 1885 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3407 3928 5604 7176 3407
Size: 521 1676 1572 4092
Fragments arranged by size:
4092 1676 1572 521
ApaLI G'TGCA_C
Cuts at: 5918 7074 5918
Size: 1156 6705
ApoI r'AATT_y
Cuts at: 1493 2744 2900 2981 4368 4734 5170 5396 5548
Size: 1251 156 81 1387 366 436 226 152
Cuts at: 5548 7657 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1387 1251 436 366 226 156
152 81
AseI AT'TA_AT
Cuts at: 160 1032 3287 6415 160
Size: 872 2255 3128 1606
Fragments arranged by size:
3128 2255 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7861
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 5186 1870
Size: 73 99 3144 4545
Fragments arranged by size:
4545 3144 99 73
AvrII C'CTAG_G
Cuts at: 2629 2629
Size: 7861
BamHI G'GATC_C
Cuts at: 5191 5558 5191
Size: 367 7494
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2373 2758 4402 5182 6637
Size: 325 217 627 586 385 1644 780 1455
Cuts at: 6637 618
Size: 1842
Fragments arranged by size:
1842 1644 1455 780 627 586 385 325
217
BanII G_rGCy'C
Cuts at: 1391 4686 4995 5180 1391
Size: 3295 309 185 4072
Fragments arranged by size:
4072 3295 309 185
BbeI G_GCGC'C
Cuts at: 2377 2377
Size: 7861
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 2517 4061 4161 928
Size: 971 446 172 1544 100 4628
Fragments arranged by size:
4628 1544 971 446 172 100
BbvCI CC'TCA_GC
Cuts at: 3251 3251
Size: 7861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6052 6086 1903
Size: 34 4115 34 3678
Fragments arranged by size:
4115 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2618 4114 5753 6963 2618
Size: 1496 1639 1210 3516
Fragments arranged by size:
3516 1639 1496 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7861
BfrBI ATG'CAT
Cuts at: 3433 3635 4241 3433
Size: 202 606 7053
Fragments arranged by size:
7053 606 202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 6472 136
Size: 107 122 71 631 605 446 4354 1525
Fragments arranged by size:
4354 1525 631 605 446 122 107 71
BglII A'GATC_T
Cuts at: 2771 3760 7856 2771
Size: 989 4096 2776
Fragments arranged by size:
4096 2776 989
BlpI GC'TnA_GC
Cuts at: 5278 5278
Size: 7861
Bme1580I G_kGCm'C
Cuts at: 1790 5922 7078 1790
Size: 4132 1156 2573
Fragments arranged by size:
4132 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7861
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5592 6550 442
Size: 416 53 83 399 24 4175 958 1753
Fragments arranged by size:
4175 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7861
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3234 3234
Size: 7861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4617 4670 4979 6521 1239
Size: 3378 53 309 1542 2579
Fragments arranged by size:
3378 2579 1542 309 53
Bpu10I CC'TnA_GC
Cuts at: 3251 4416 3251
Size: 1165 6696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5986 6851 7149 5986
Size: 865 298 6698
Fragments arranged by size:
6698 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6524 882
Size: 617 5025 2219
Fragments arranged by size:
5025 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5652 493
Size: 711 4448 2702
Fragments arranged by size:
4448 2702 711
BsaBI GATnn'nnATC
Cuts at: 3432 3747 3924 7855 3432
Size: 315 177 3931 3438
Fragments arranged by size:
3931 3438 315 177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2169 2374 5668
Size: 53 83 186 936 588 48 205 3294
Cuts at: 5668 6050 6685 275
Size: 382 635 1451
Fragments arranged by size:
3294 1451 936 635 588 382 205 186
83 53 48
BsaWI w'CCGG_w
Cuts at: 6294 6692 6966 7113 7524 6294
Size: 398 274 147 411 6631
Fragments arranged by size:
6631 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7636
Fragments arranged by size:
7636 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 4281 4935 5014 1186
Size: 1279 1816 654 79 4033
Fragments arranged by size:
4033 1816 1279 654 79
BseYI C'CCAG_C
Cuts at: 7064 7560 7064
Size: 496 7365
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2076 5311 6072 7100 1540
Size: 275 186 18 57 3235 761 1028 2301
Fragments arranged by size:
3235 2301 1028 761 275 186 57 18
BsiHKAI G_wGCw'C
Cuts at: 3784 4686 4995 5922 6007 7078 3784
Size: 902 309 927 85 1071 4567
Fragments arranged by size:
4567 1071 927 902 309 85
BsmI GAATG_Cn'
Cuts at: 4181 5372 5465 4181
Size: 1191 93 6577
Fragments arranged by size:
6577 1191 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2814 2814
Size: 7861
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3784 4686 4995 5180 5922 6007 7078
Size: 399 1994 902 309 185 742 85 1071
Cuts at: 7078 1391
Size: 2174
Fragments arranged by size:
2174 1994 1071 902 742 399 309 185
85
BspEI T'CCGG_A
Cuts at: 7524 7524
Size: 7861
BspHI T'CATG_A
Cuts at: 5750 7480 5750
Size: 1730 6131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2335 3576 5216 844
Size: 1491 1241 1640 3489
Fragments arranged by size:
3489 1640 1491 1241
BsrBI CCG'CTC
Cuts at: 1394 1823 5748 1394
Size: 429 3925 3507
Fragments arranged by size:
3925 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4992 6356 6530 65
Size: 1183 3744 1364 174 1396
Fragments arranged by size:
3744 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6505 6692 1700
Size: 110 4695 187 2869
Fragments arranged by size:
4695 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7861
BssHII G'CGCG_C
Cuts at: 1925 2049 1925
Size: 124 7737
BssSI C'ACGA_G
Cuts at: 3504 5921 6933 3504
Size: 2417 1012 4432
Fragments arranged by size:
4432 2417 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7861
BstBI TT'CG_AA
Cuts at: 4146 6665 4146
Size: 2519 5342
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7861
BstYI r'GATC_y
Cuts at: 1972 2233 2771 3760 5191 5558 5943 5960 7412
Size: 261 538 989 1431 367 385 17 1452
Cuts at: 7412 7498 7510 7856 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1431 989 538 385 367 346
261 86 17 12
BstZ17I GTA'TAC
Cuts at: 3276 3276
Size: 7861
BtgI C'CryG_G
Cuts at: 513 1065 2013 513
Size: 552 948 6361
Fragments arranged by size:
6361 948 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7122
Fragments arranged by size:
7122 683 56
BtsI GCAGTG_nn'
Cuts at: 5377 6171 6191 5377
Size: 794 20 7047
Fragments arranged by size:
7047 794 20
ClaI AT'CG_AT
Cuts at: 1554 4026 1554
Size: 2472 5389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7826
DraI TTT'AAA
Cuts at: 5165 5165
Size: 7861
DraIII CAC_nnn'GTG
Cuts at: 4191 4191
Size: 7861
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6868 809
Size: 507 5552 1802
Fragments arranged by size:
5552 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2148 5308
Size: 54 1425 50 275 18 186 132 3160
Cuts at: 5308 6197 7826 8
Size: 889 1629 43
Fragments arranged by size:
3160 1629 1425 889 275 186 132 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 5308 1537
Size: 275 204 3292 4090
Fragments arranged by size:
4090 3292 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 2646 2661 3027 4263 4485 5791 1353
Size: 1101 192 15 366 1236 222 1306 3423
Fragments arranged by size:
3423 1306 1236 1101 366 222 192 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2016 6448 6822 6968 387
Size: 1064 138 427 4432 374 146 1280
Fragments arranged by size:
4432 1280 1064 427 374 146 138
EcoICRI GAG'CTC
Cuts at: 4684 4993 4684
Size: 309 7552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3008 3502 3749 4617 4670 4869 4979
Size: 393 1376 494 247 868 53 199 110
Cuts at: 4979 5924 6521 7308 7576 1239
Size: 945 597 787 268 1524
Fragments arranged by size:
1524 1376 945 868 787 597 494 393
268 247 199 110 53
EcoRI G'AATT_C
Cuts at: 2744 2981 4734 5170 5548 2744
Size: 237 1753 436 378 5057
Fragments arranged by size:
5057 1753 436 378 237
EcoRV GAT'ATC
Cuts at: 4024 4024
Size: 7861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2826 3779 3929 4472 4529 2422
Size: 404 953 150 543 57 5754
Fragments arranged by size:
5754 953 543 404 150 57
FspI TGC'GCA
Cuts at: 6367 6367
Size: 7861
HaeII r_GCGC'y
Cuts at: 1678 2157 2377 7008 1678
Size: 479 220 4631 2531
Fragments arranged by size:
4631 2531 479 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2504 2536 2568
Size: 32 135 32 611 32 289 32 32
Cuts at: 2568 3032 3064 3498 3530 4112 4144 4693 4725
Size: 464 32 434 32 582 32 549 32
Cuts at: 4725 6502 6534 6576 6608 1373
Size: 1777 32 42 32 2626
Fragments arranged by size:
2626 1777 611 582 549 464 434 289
135 42 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5205 5451 669
Size: 988 281 3267 246 3079
Fragments arranged by size:
3267 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4511 748
Size: 3763 4098
HpaI GTT'AAC
Cuts at: 5451 5451
Size: 7861
KasI G'GCGC_C
Cuts at: 2373 2373
Size: 7861
KpnI G_GTAC'C
Cuts at: 2762 4406 5186 2762
Size: 1644 780 5437
Fragments arranged by size:
5437 1644 780
MfeI C'AATT_G
Cuts at: 4534 5460 4534
Size: 926 6935
MluI A'CGCG_T
Cuts at: 3680 7737 3680
Size: 4057 3804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2369 3570 6975 7159 1305
Size: 1064 1201 3405 184 2007
Fragments arranged by size:
3405 2007 1201 1064 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6039
Fragments arranged by size:
6039 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3353 5819 6178 6337 518
Size: 648 2187 2466 359 159 2042
Fragments arranged by size:
2466 2187 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2082 4658 4807 5277 5954 7102 7347
Size: 409 67 2576 149 470 677 1148 245
Cuts at: 7347 1606
Size: 2120
Fragments arranged by size:
2576 2120 1148 677 470 409 245 149
67
NarI GG'CG_CC
Cuts at: 2374 2374
Size: 7861
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7309
NdeI CA'TA_TG
Cuts at: 387 2700 3159 4819 387
Size: 2313 459 1660 3429
Fragments arranged by size:
3429 2313 1660 459
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7861
NotI GC'GGCC_GC
Cuts at: 5308 5308
Size: 7861
NsiI A_TGCA'T
Cuts at: 3435 3637 4243 3435
Size: 202 606 7053
Fragments arranged by size:
7053 606 202
NspI r_CATG'y
Cuts at: 1132 1229 3565 3986 5219 6764 1132
Size: 97 2336 421 1233 1545 2229
Fragments arranged by size:
2336 2229 1545 1233 421 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7861
PciI A'CATG_T
Cuts at: 3982 6760 3982
Size: 2778 5083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7861
PfoI T'CCnGG_A
Cuts at: 4686 4686
Size: 7861
PmeI GTTT'AAAC
Cuts at: 5165 5165
Size: 7861
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5930 5962 7443 7475 5930
Size: 32 1481 32 6316
Fragments arranged by size:
6316 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7861
PsiI TTA'TAA
Cuts at: 3827 4140 5431 3827
Size: 313 1291 6257
Fragments arranged by size:
6257 1291 313
PstI C_TGCA'G
Cuts at: 830 3587 4063 5213 830
Size: 2757 476 1150 3478
Fragments arranged by size:
3478 2757 1150 476
PvuI CG_AT'CG
Cuts at: 2001 2076 2001
Size: 75 7786
PvuII CAG'CTG
Cuts at: 4658 4807 4658
Size: 149 7712
SacI G_AGCT'C
Cuts at: 4686 4995 4686
Size: 309 7552
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 7861
SalI G'TCGA_C
Cuts at: 1936 5203 1936
Size: 3267 4594
SapI GCTCTTCn'nnn_
Cuts at: 2454 4263 2454
Size: 1809 6052
SbfI CC_TGCA'GG
Cuts at: 5213 5213
Size: 7861
ScaI AGT'ACT
Cuts at: 3510 3581 6109 7821 3510
Size: 71 2528 1712 3550
Fragments arranged by size:
3550 2528 1712 71
SexAI A'CCwGG_T
Cuts at: 3406 3406
Size: 7861
SfcI C'TryA_G
Cuts at: 826 1045 3583 3840 4059 4159 5209 6344 7025
Size: 219 2538 257 219 100 1050 1135 681
Cuts at: 7025 7216 826
Size: 191 1471
Fragments arranged by size:
2538 1471 1135 1050 681 257 219 219
191 100
SfoI GGC'GCC
Cuts at: 2375 2375
Size: 7861
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7861
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7861
SmaI CCC'GGG
Cuts at: 1872 5188 1872
Size: 3316 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4970 5965 6866 7128 820
Size: 197 926 3027 995 901 262 1553
Fragments arranged by size:
3027 1553 995 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5652 493
Size: 5159 2702
SpeI A'CTAG_T
Cuts at: 152 3142 5158 152
Size: 2990 2016 2855
Fragments arranged by size:
2990 2855 2016
SphI G_CATG'C
Cuts at: 1132 5219 1132
Size: 4087 3774
SspI AAT'ATT
Cuts at: 5 52 2187 4652 5785 5
Size: 47 2135 2465 1133 2081
Fragments arranged by size:
2465 2135 2081 1133 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7861
StyI C'CwwG_G
Cuts at: 513 1065 2629 3127 4284 5300 513
Size: 552 1564 498 1157 1016 3074
Fragments arranged by size:
3074 1564 1157 1016 552 498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3792 4297 5888 5905 6058 6243 6688
Size: 1255 2212 505 1591 17 153 185 445
Cuts at: 6688 325
Size: 1498
Fragments arranged by size:
2212 1591 1498 1255 505 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3508 3579 6107
Size: 274 80 33 51 1443 1531 71 2528
Cuts at: 6107 7819 96
Size: 1712 138
Fragments arranged by size:
2528 1712 1531 1443 274 138 80 71
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7821 7853 7821
Size: 32 7829
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7861
XbaI T'CTAG_A
Cuts at: 5197 5197
Size: 7861
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4984 4984
Size: 7861
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7861
XmaI C'CCGG_G
Cuts at: 1870 5186 1870
Size: 3316 4545
XmnI GAAnn'nnTTC
Cuts at: 3954 4257 5990 3954
Size: 303 1733 5825
Fragments arranged by size:
5825 1733 303
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5669 276
Size: 53 83 186 936 4135 2468
Fragments arranged by size:
4135 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BsiWI BstXI Bsu36I EcoNI
EcoO109I FseI FspAI NaeI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SanDI SfiI SrfI
SwaI