Restriction map for
pFN21AB1569
(Circular) (Six-base) MAPSORT of: pFN21AB1569.seq Check: 8969 from: 1 to: 7654
pFN21AB1569 7654 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2907 3881 2907
Size: 974 6680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5464 278
Size: 53 83 186 936 3928 2468
Fragments arranged by size:
3928 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2595 2824 4997 1937
Size: 658 229 2173 4594
Fragments arranged by size:
4594 2173 658 229
Acc65I G'GTAC_C
Cuts at: 2650 4975 2650
Size: 2325 5329
AclI AA'CG_TT
Cuts at: 1563 5781 6154 1563
Size: 4218 373 3063
Fragments arranged by size:
4218 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 4688 5717 7101 7369 1632
Size: 878 2178 1029 1384 268 1917
Fragments arranged by size:
2178 1917 1384 1029 878 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7457
AflIII A'CryG_T
Cuts at: 1203 6553 7530 1203
Size: 5350 977 1327
Fragments arranged by size:
5350 1327 977
AgeI A'CCGG_T
Cuts at: 6485 6485
Size: 7654
AhdI GACnn_n'nnGTC
Cuts at: 2665 6383 2665
Size: 3718 3936
AleI CACnn'nnGTG
Cuts at: 1166 2155 3182 4805 1166
Size: 989 1027 1623 4015
Fragments arranged by size:
4015 1623 1027 989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7654
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2288 2320 2288
Size: 32 7622
AlwNI CAG_nnn'CTG
Cuts at: 2671 3548 4334 5397 6969 2671
Size: 877 786 1063 1572 3356
Fragments arranged by size:
3356 1572 1063 877 786
ApaLI G'TGCA_C
Cuts at: 5711 6867 5711
Size: 1156 6498
ApoI r'AATT_y
Cuts at: 1493 2405 3896 4963 5189 5341 7450 1493
Size: 912 1491 1067 226 152 2109 1697
Fragments arranged by size:
2109 1697 1491 1067 912 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6208 160
Size: 872 5176 1606
Fragments arranged by size:
5176 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7654
AvaI C'yCGr_G
Cuts at: 1870 1943 4979 1870
Size: 73 3036 4545
Fragments arranged by size:
4545 3036 73
BamHI G'GATC_C
Cuts at: 4984 5351 4984
Size: 367 7287
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2650 4975 6430 618
Size: 325 217 627 863 2325 1455 1842
Fragments arranged by size:
2325 1842 1455 863 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2301 3263 3538 4973 1391
Size: 910 962 275 1435 4072
Fragments arranged by size:
4072 1435 962 910 275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3819 928
Size: 971 1920 4763
Fragments arranged by size:
4763 1920 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5845 5879 1903
Size: 34 3908 34 3678
Fragments arranged by size:
3908 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5546 6756 5546
Size: 1210 6444
BclI T'GATC_A
Cuts at: 1548 2392 4263 1548
Size: 844 1871 4939
Fragments arranged by size:
4939 1871 844
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6265 136
Size: 107 122 71 631 605 4593 1525
Fragments arranged by size:
4593 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7649 7649
Size: 7654
BlpI GC'TnA_GC
Cuts at: 3257 5071 3257
Size: 1814 5840
Bme1580I G_kGCm'C
Cuts at: 1790 2612 2876 5715 6871 1790
Size: 822 264 2839 1156 2573
Fragments arranged by size:
2839 2573 1156 822 264
BmgBI CAC'GTC
Cuts at: 1318 4410 1318
Size: 3092 4562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 2659 4147
Size: 416 53 83 399 24 852 390 1488
Cuts at: 4147 4489 4937 5385 6343 442
Size: 342 448 448 958 1753
Fragments arranged by size:
1753 1488 958 852 448 448 416 399
390 342 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7654
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 7654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3058 3334 4337 6314 1239
Size: 1819 276 1003 1977 2579
Fragments arranged by size:
2579 1977 1819 1003 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 3485 5779 6644 6942 2285
Size: 1200 2294 865 298 2997
Fragments arranged by size:
2997 2294 1200 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3011 3785 4233 4465 6317 882
Size: 617 1512 774 448 232 1852 2219
Fragments arranged by size:
2219 1852 1512 774 617 448 232
BsaAI yAC'GTr
Cuts at: 493 1204 3520 4139 5445 493
Size: 711 2316 619 1306 2702
Fragments arranged by size:
2702 2316 1306 711 619
BsaBI GATnn'nnATC
Cuts at: 2391 4364 7648 2391
Size: 1973 3284 2397
Fragments arranged by size:
3284 2397 1973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5461 5843 6478 275
Size: 53 83 186 936 3928 382 635 1451
Fragments arranged by size:
3928 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4650 6087 6485 6759 6906 7317 4650
Size: 1437 398 274 147 411 4987
Fragments arranged by size:
4987 1437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2288 2318 2962 2992 3587
Size: 30 165 30 885 30 644 30 595
Cuts at: 3587 3617 3691 3721 4442 4472 1178
Size: 30 74 30 721 30 4360
Fragments arranged by size:
4360 885 721 644 595 165 74 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 3659 4009 4365 1186
Size: 832 1641 350 356 4475
Fragments arranged by size:
4475 1641 832 356 350
BseYI C'CCAG_C
Cuts at: 2128 4552 6857 7353 2128
Size: 2424 2305 496 2429
Fragments arranged by size:
2429 2424 2305 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3931 4704 1163
Size: 573 2195 773 4113
Fragments arranged by size:
4113 2195 773 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5104 5865 6893 1540
Size: 275 186 3103 761 1028 2301
Fragments arranged by size:
3103 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 3263 5715 5800 6871 2301
Size: 962 2452 85 1071 3084
Fragments arranged by size:
3084 2452 1071 962 85
BsmI GAATG_Cn'
Cuts at: 2328 5165 5258 2328
Size: 2837 93 4724
Fragments arranged by size:
4724 2837 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2612 2876 3263 3538 4973 5715
Size: 399 511 311 264 387 275 1435 742
Cuts at: 5715 5800 6871 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1435 1071 742 511 399 387 311
275 264 85
BspEI T'CCGG_A
Cuts at: 4650 7317 4650
Size: 2667 4987
BspHI T'CATG_A
Cuts at: 3633 3832 5543 7273 3633
Size: 199 1711 1730 4014
Fragments arranged by size:
4014 1730 1711 199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2907 3881 5009 844
Size: 2063 974 1128 3489
Fragments arranged by size:
3489 2063 1128 974
BsrBI CCG'CTC
Cuts at: 1394 1823 5541 1394
Size: 429 3718 3507
Fragments arranged by size:
3718 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2224 2439 2788 3763 4729 6149 6323
Size: 1183 976 215 349 975 966 1420 174
Cuts at: 6323 65
Size: 1396
Fragments arranged by size:
1420 1396 1183 976 975 966 349 215
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6298 6485 1700
Size: 110 4488 187 2869
Fragments arranged by size:
4488 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7654
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7654
BssSI C'ACGA_G
Cuts at: 5714 6726 5714
Size: 1012 6642
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7654
BstBI TT'CG_AA
Cuts at: 6458 6458
Size: 7654
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7654
BstXI CCAn_nnnn'nTGG
Cuts at: 3850 4371 3850
Size: 521 7133
BstYI r'GATC_y
Cuts at: 1972 2136 2470 2716 4359 4984 5351 5736 5753
Size: 164 334 246 1643 625 367 385 17
Cuts at: 5753 7205 7291 7303 7649 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1643 1452 625 385 367 346 334
246 164 86 17 12
BstZ17I GTA'TAC
Cuts at: 2596 2596
Size: 7654
BtgI C'CryG_G
Cuts at: 513 1065 2004 2655 2961 3180 4542 4875 4950
Size: 552 939 651 306 219 1362 333 75
Cuts at: 4950 513
Size: 3217
Fragments arranged by size:
3217 1362 939 651 552 333 306 219
75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4434 487
Size: 683 56 3208 3707
Fragments arranged by size:
3707 3208 683 56
BtsI GCAGTG_nn'
Cuts at: 3919 4783 5170 5964 5984 3919
Size: 864 387 794 20 5589
Fragments arranged by size:
5589 864 794 387 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7619
DraI TTT'AAA
Cuts at: 3768 4958 3768
Size: 1190 6464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3416 6661 809
Size: 507 2100 3245 1802
Fragments arranged by size:
3245 2100 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3954 5101 5990
Size: 54 1425 50 275 18 2124 1147 889
Cuts at: 5990 7619 8
Size: 1629 43
Fragments arranged by size:
2124 1629 1425 1147 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5101 1537
Size: 275 3289 4090
Fragments arranged by size:
4090 3289 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 5584 1353
Size: 804 3427 3423
Fragments arranged by size:
3427 3423 804
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6241 6615 6761 387
Size: 1064 138 4652 374 146 1280
Fragments arranged by size:
4652 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2299 3261 2299
Size: 962 6692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 3058 3334 4337 4688 5717 6314
Size: 393 878 548 276 1003 351 1029 597
Cuts at: 6314 7101 7369 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1029 1003 878 787 597 548 393
351 276 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2675 2675
Size: 7654
EcoO109I rG'GnC_Cy
Cuts at: 2263 2519 2263
Size: 256 7398
EcoRI G'AATT_C
Cuts at: 4963 5341 4963
Size: 378 7276
EcoRV GAT'ATC
Cuts at: 4732 4732
Size: 7654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4813 4813
Size: 7654
FspI TGC'GCA
Cuts at: 6160 6160
Size: 7654
HaeII r_GCGC'y
Cuts at: 1678 6801 1678
Size: 5123 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 2651 2683 2942
Size: 32 135 32 556 32 491 32 259
Cuts at: 2942 2974 3332 3364 3587 3619 4344 4376 4442
Size: 32 358 32 223 32 725 32 66
Cuts at: 4442 4474 4477 4509 6295 6327 6369 6401 1373
Size: 32 3 32 1786 32 42 32 2626
Fragments arranged by size:
2626 1786 725 556 491 358 259 223
135 66 42 32 32 32 32 32
32 32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 2698 2825 3796 4998 5244
Size: 988 281 589 171 127 971 1202 246
Cuts at: 5244 669
Size: 3079
Fragments arranged by size:
3079 1202 988 971 589 281 246 171
127
HindIII A'AGCT_T
Cuts at: 748 2817 4643 748
Size: 2069 1826 3759
Fragments arranged by size:
3759 2069 1826
HpaI GTT'AAC
Cuts at: 5244 5244
Size: 7654
KpnI G_GTAC'C
Cuts at: 2654 4979 2654
Size: 2325 5329
MfeI C'AATT_G
Cuts at: 2242 5253 2242
Size: 3011 4643
MluI A'CGCG_T
Cuts at: 7530 7530
Size: 7654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3865 6768 6952 1305
Size: 2560 2903 184 2007
Fragments arranged by size:
2903 2560 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3956 10
Size: 54 1425 343 2124 3708
Fragments arranged by size:
3708 2124 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2155 2465 3182 3393 3807 4805 5612
Size: 648 989 310 717 211 414 998 807
Cuts at: 5612 5971 6130 518
Size: 359 159 2042
Fragments arranged by size:
2042 998 989 807 717 648 414 359
310 211 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2447 3548 3650 4556 4570 5070 5747
Size: 434 407 1101 102 906 14 500 677
Cuts at: 5747 6895 7140 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1101 906 677 500 434 407
245 102 14
NcoI C'CATG_G
Cuts at: 513 1065 2004 2655 2961 4542 513
Size: 552 939 651 306 1581 3625
Fragments arranged by size:
3625 1581 939 651 552 306
NdeI CA'TA_TG
Cuts at: 387 3089 3808 387
Size: 2702 719 4233
Fragments arranged by size:
4233 2702 719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7654
NotI GC'GGCC_GC
Cuts at: 5101 5101
Size: 7654
NspI r_CATG'y
Cuts at: 1132 1229 5012 6557 1132
Size: 97 3783 1545 2229
Fragments arranged by size:
3783 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7654
PciI A'CATG_T
Cuts at: 6553 6553
Size: 7654
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2153 2671 1108
Size: 1045 518 6091
Fragments arranged by size:
6091 1045 518
PfoI T'CCnGG_A
Cuts at: 3030 4314 3030
Size: 1284 6370
PmeI GTTT'AAAC
Cuts at: 4958 4958
Size: 7654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2288 2320 5723 5755 7236 7268 2288
Size: 32 3403 32 1481 32 2674
Fragments arranged by size:
3403 2674 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2263 2519 2263
Size: 256 7398
PshAI GACnn'nnGTC
Cuts at: 1613 2232 1613
Size: 619 7035
PsiI TTA'TAA
Cuts at: 5224 5224
Size: 7654
PstI C_TGCA'G
Cuts at: 830 2738 2918 5006 830
Size: 1908 180 2088 3478
Fragments arranged by size:
3478 2088 1908 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7654
PvuII CAG'CTG
Cuts at: 2040 2447 4556 4570 2040
Size: 407 2109 14 5124
Fragments arranged by size:
5124 2109 407 14
SacI G_AGCT'C
Cuts at: 2301 3263 2301
Size: 962 6692
SalI G'TCGA_C
Cuts at: 1936 2823 4996 1936
Size: 887 2173 4594
Fragments arranged by size:
4594 2173 887
SanDI GG'GwC_CC
Cuts at: 2263 2263
Size: 7654
SbfI CC_TGCA'GG
Cuts at: 5006 5006
Size: 7654
ScaI AGT'ACT
Cuts at: 5902 7614 5902
Size: 1712 5942
SexAI A'CCwGG_T
Cuts at: 2208 2827 2208
Size: 619 7035
SfcI C'TryA_G
Cuts at: 826 1045 2734 2914 4389 4512 5002 6137 6818
Size: 219 1689 180 1475 123 490 1135 681
Cuts at: 6818 7009 826
Size: 191 1471
Fragments arranged by size:
1689 1475 1471 1135 681 490 219 191
180 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7654
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7654
SmaI CCC'GGG
Cuts at: 1872 4981 1872
Size: 3109 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 3500 5758 6659 6921 820
Size: 197 926 357 1200 2258 901 262 1553
Fragments arranged by size:
2258 1553 1200 926 901 357 262 197
SnaBI TAC'GTA
Cuts at: 493 3520 4139 5445 493
Size: 3027 619 1306 2702
Fragments arranged by size:
3027 2702 1306 619
SpeI A'CTAG_T
Cuts at: 152 3587 152
Size: 3435 4219
SphI G_CATG'C
Cuts at: 1132 5012 1132
Size: 3880 3774
SspI AAT'ATT
Cuts at: 5 52 5578 5
Size: 47 5526 2081
Fragments arranged by size:
5526 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7654
StyI C'CwwG_G
Cuts at: 513 1065 2004 2577 2655 2723 2961 3843 4122
Size: 552 939 573 78 68 238 882 279
Cuts at: 4122 4542 5093 513
Size: 420 551 3074
Fragments arranged by size:
3074 939 882 573 552 551 420 279
238 78 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2145 2209 3093 5681 5698 5851 6036
Size: 1255 565 64 884 2588 17 153 185
Cuts at: 6036 6481 325
Size: 445 1498
Fragments arranged by size:
2588 1498 1255 884 565 445 185 153
64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 2730 2738
Size: 274 80 33 51 1443 393 360 8
Cuts at: 2738 4812 5900 7612 96
Size: 2074 1088 1712 138
Fragments arranged by size:
2074 1712 1443 1088 393 360 274 138
80 51 33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3691 3723 7614 7646 3691
Size: 32 3891 32 3699
Fragments arranged by size:
3891 3699 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7654
XbaI T'CTAG_A
Cuts at: 4918 4990 4918
Size: 72 7582
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2154 2154
Size: 7654
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7654
XmaI C'CCGG_G
Cuts at: 1870 4979 1870
Size: 3109 4545
XmnI GAAnn'nnTTC
Cuts at: 2070 5783 2070
Size: 3713 3941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5462 276
Size: 53 83 186 936 3928 2468
Fragments arranged by size:
3928 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI
SwaI