Restriction map for
pFN21AB1529
(Circular) (Six-base) MAPSORT of: pFN21AB1529.seq Check: 6283 from: 1 to: 8611
pFN21AB1529 8611 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2090 2090
Size: 8611
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6421 278
Size: 53 83 186 936 4885 2468
Fragments arranged by size:
4885 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 5954 1937
Size: 2292 1725 4594
Fragments arranged by size:
4594 2292 1725
Acc65I G'GTAC_C
Cuts at: 2768 5932 2768
Size: 3164 5447
AclI AA'CG_TT
Cuts at: 1563 6738 7111 1563
Size: 5175 373 3063
Fragments arranged by size:
5175 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2694 2781 3207 4231 4293 4509 5630 6674
Size: 1062 87 426 1024 62 216 1121 1044
Cuts at: 6674 8058 8326 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1121 1062 1044 1024 426 268
216 87 62
AflII C'TTAA_G
Cuts at: 820 1017 4544 820
Size: 197 3527 4887
Fragments arranged by size:
4887 3527 197
AflIII A'CryG_T
Cuts at: 1203 5012 7510 8487 1203
Size: 3809 2498 977 1327
Fragments arranged by size:
3809 2498 1327 977
AgeI A'CCGG_T
Cuts at: 3196 7442 3196
Size: 4246 4365
AhdI GACnn_n'nnGTC
Cuts at: 3263 7340 3263
Size: 4077 4534
AleI CACnn'nnGTG
Cuts at: 1166 5579 1166
Size: 4413 4198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4620 1184
Size: 3436 5175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 8579
AlwNI CAG_nnn'CTG
Cuts at: 2024 3230 3701 3956 4981 5608 6354 7926 2024
Size: 1206 471 255 1025 627 746 1572 2709
Fragments arranged by size:
2709 1572 1206 1025 746 627 471 255
ApaLI G'TGCA_C
Cuts at: 6668 7824 6668
Size: 1156 7455
ApoI r'AATT_y
Cuts at: 1493 2527 3683 4019 4352 5111 5920 6146 6298
Size: 1034 1156 336 333 759 809 226 152
Cuts at: 6298 8407 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1156 1034 809 759 336 333
226 152
AseI AT'TA_AT
Cuts at: 160 1032 7165 160
Size: 872 6133 1606
Fragments arranged by size:
6133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8611
AvaI C'yCGr_G
Cuts at: 1870 1943 5770 5936 1870
Size: 73 3827 166 4545
Fragments arranged by size:
4545 3827 166 73
AvrII C'CTAG_G
Cuts at: 2290 2692 2290
Size: 402 8209
BamHI G'GATC_C
Cuts at: 2851 3407 4757 5941 6308 2851
Size: 556 1350 1184 367 5154
Fragments arranged by size:
5154 1350 1184 556 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2768 5036 5826 5932 7387
Size: 325 217 627 981 2268 790 106 1455
Cuts at: 7387 618
Size: 1842
Fragments arranged by size:
2268 1842 1455 981 790 627 325 217
106
BanII G_rGCy'C
Cuts at: 1391 3239 5930 1391
Size: 1848 2691 4072
Fragments arranged by size:
4072 2691 1848
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4237 4293 4425 928
Size: 971 2338 56 132 5114
Fragments arranged by size:
5114 2338 971 132 56
BbvCI CC'TCA_GC
Cuts at: 3248 4765 3248
Size: 1517 7094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6802 6836 1903
Size: 34 4865 34 3678
Fragments arranged by size:
4865 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2727 2979 6503 7713 2727
Size: 252 3524 1210 3625
Fragments arranged by size:
3625 3524 1210 252
BclI T'GATC_A
Cuts at: 1548 5166 1548
Size: 3618 4993
BfrBI ATG'CAT
Cuts at: 5387 5387
Size: 8611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7222 136
Size: 107 122 71 631 605 5550 1525
Fragments arranged by size:
5550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3689 4444 4540 8606 3689
Size: 755 96 4066 3694
Fragments arranged by size:
4066 3694 755 96
BlpI GC'TnA_GC
Cuts at: 2859 6028 2859
Size: 3169 5442
Bme1580I G_kGCm'C
Cuts at: 1790 3490 5419 5829 6672 7828 1790
Size: 1700 1929 410 843 1156 2573
Fragments arranged by size:
2573 1929 1700 1156 843 410
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2715 6342 7300
Size: 416 53 83 399 24 1298 3627 958
Cuts at: 7300 442
Size: 1753
Fragments arranged by size:
3627 1753 1298 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5771 5771
Size: 8611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 2325 2553 3621 4330 5250 5582 7271
Size: 1068 18 228 1068 709 920 332 1689
Cuts at: 7271 1239
Size: 2579
Fragments arranged by size:
2579 1689 1068 1068 920 709 332 228
18
Bpu10I CC'TnA_GC
Cuts at: 3025 3248 4765 5730 3025
Size: 223 1517 965 5906
Fragments arranged by size:
5906 1517 965 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2103 5028 5484 6736 7601 7899 2103
Size: 2925 456 1252 865 298 2815
Fragments arranged by size:
2925 2815 1252 865 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2167 4871 5283 7274 882
Size: 617 668 2704 412 1991 2219
Fragments arranged by size:
2704 2219 1991 668 617 412
BsaAI yAC'GTr
Cuts at: 493 1204 5013 6402 493
Size: 711 3809 1389 2702
Fragments arranged by size:
3809 2702 1389 711
BsaBI GATnn'nnATC
Cuts at: 8605 8605
Size: 8611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2425 6418 6800 7435
Size: 53 83 186 936 892 3993 382 635
Cuts at: 7435 275
Size: 1451
Fragments arranged by size:
3993 1451 936 892 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3196 7044 7442 7716 7863 8274 3196
Size: 3848 398 274 147 411 3533
Fragments arranged by size:
3848 3533 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2627 2923 2953 4328
Size: 30 165 30 1194 30 296 30 1375
Cuts at: 4328 4358 4997 5027 1178
Size: 30 639 30 4762
Fragments arranged by size:
4762 1375 1194 639 296 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 4551 5233 5788 1186
Size: 1435 1930 682 555 4009
Fragments arranged by size:
4009 1930 1435 682 555
BseYI C'CCAG_C
Cuts at: 3145 3471 4888 5018 5023 5560 7814 8310 3145
Size: 326 1417 130 5 537 2254 496 3446
Fragments arranged by size:
3446 2254 1417 537 496 326 130 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5605 1163
Size: 573 3869 4169
Fragments arranged by size:
4169 3869 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6061 6822 7850 1540
Size: 275 186 4060 761 1028 2301
Fragments arranged by size:
4060 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4673 6672 6757 7828 4673
Size: 1999 85 1071 5456
Fragments arranged by size:
5456 1999 1071 85
BsmI GAATG_Cn'
Cuts at: 2286 2874 5783 6122 6215 2286
Size: 588 2909 339 93 4682
Fragments arranged by size:
4682 2909 588 339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3239 3490 4673 5419 5829 5930 6672
Size: 399 1449 251 1183 746 410 101 742
Cuts at: 6672 6757 7828 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1449 1183 1071 746 742 410 399
251 101 85
BspEI T'CCGG_A
Cuts at: 8274 8274
Size: 8611
BspHI T'CATG_A
Cuts at: 6500 8230 6500
Size: 1730 6881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2090 4989 5913 5966 844
Size: 1246 2899 924 53 3489
Fragments arranged by size:
3489 2899 1246 924 53
BsrBI CCG'CTC
Cuts at: 1394 1823 6498 1394
Size: 429 4675 3507
Fragments arranged by size:
4675 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3123 3524 7106 7280 65
Size: 1183 1875 401 3582 174 1396
Fragments arranged by size:
3582 1875 1396 1183 401 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3196 7255 7442 1700
Size: 110 1386 4059 187 2869
Fragments arranged by size:
4059 2869 1386 187 110
BsrGI T'GTAC_A
Cuts at: 96 5535 96
Size: 5439 3172
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8611
BssSI C'ACGA_G
Cuts at: 6671 7683 6671
Size: 1012 7599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8611
BstBI TT'CG_AA
Cuts at: 7415 7415
Size: 8611
BstEII G'GTnAC_C
Cuts at: 1184 5542 1184
Size: 4358 4253
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 4186 5554 2728
Size: 1458 1368 5785
Fragments arranged by size:
5785 1458 1368
BstYI r'GATC_y
Cuts at: 1972 2851 3407 3604 3689 4444 4540 4757 5941
Size: 879 556 197 85 755 96 217 1184
Cuts at: 5941 6308 6693 6710 8162 8248 8260 8606 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1184 879 755 556 385 367
346 217 197 96 86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 3793 3793
Size: 8611
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 7078
Fragments arranged by size:
7078 939 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2559 487
Size: 683 56 1333 6539
Fragments arranged by size:
6539 1333 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 2775 4401 4474 4671 5574 5586 5593 6127
Size: 302 1626 73 197 903 12 7 534
Cuts at: 6127 6921 6941 2473
Size: 794 20 4143
Fragments arranged by size:
4143 1626 903 794 534 302 197 73
20 12 7
ClaI AT'CG_AT
Cuts at: 1554 5619 1554
Size: 4065 4546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8576
DraI TTT'AAA
Cuts at: 3135 3556 3724 4906 5915 3135
Size: 421 168 1182 1009 5831
Fragments arranged by size:
5831 1182 1009 421 168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4225 7618 809
Size: 507 2909 3393 1802
Fragments arranged by size:
3393 2909 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2680 2730
Size: 54 1425 50 275 18 185 665 50
Cuts at: 2730 4591 6058 6947 8576 8
Size: 1861 1467 889 1629 43
Fragments arranged by size:
1861 1629 1467 1425 889 665 275 185
54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6058 1537
Size: 275 4246 4090
Fragments arranged by size:
4246 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2430 2486 2814 4875 6541 1353
Size: 1008 69 56 328 2061 1666 3423
Fragments arranged by size:
3423 2061 1666 1008 328 69 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2056 7198 7572 7718 387
Size: 1064 138 467 5142 374 146 1280
Fragments arranged by size:
5142 1280 1064 467 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2325 2553 2694 2781 3207 3621
Size: 393 675 18 228 141 87 426 414
Cuts at: 3621 4231 4293 4330 4509 5250 5582 5630 6674
Size: 610 62 37 179 741 332 48 1044
Cuts at: 6674 7271 8058 8326 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1044 787 741 675 610 597 426
414 393 332 268 228 179 141 87
62 48 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2097 2121 2289 4151 2097
Size: 24 168 1862 6557
Fragments arranged by size:
6557 1862 168 24
EcoO109I rG'GnC_Cy
Cuts at: 2228 2594 3227 4978 2228
Size: 366 633 1751 5861
Fragments arranged by size:
5861 1751 633 366
EcoRI G'AATT_C
Cuts at: 3683 4019 5920 6298 3683
Size: 336 1901 378 5996
Fragments arranged by size:
5996 1901 378 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2938 3083 4886 5271 2938
Size: 145 1803 385 6278
Fragments arranged by size:
6278 1803 385 145
FspI TGC'GCA
Cuts at: 7117 7117
Size: 8611
HaeII r_GCGC'y
Cuts at: 1678 2012 7758 1678
Size: 334 5746 2531
Fragments arranged by size:
5746 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3249 3281 3563 3595 3725
Size: 32 135 32 1677 32 282 32 130
Cuts at: 3725 3757 4328 4360 4412 4444 4484 4516 5666
Size: 32 571 32 52 32 40 32 1150
Cuts at: 5666 5681 5698 5713 7252 7284 7326 7358 1373
Size: 15 17 15 1539 32 42 32 2626
Fragments arranged by size:
2626 1677 1539 1150 571 282 135 130
52 42 40 32 32 32 32 32
32 32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3948 5955 6201 669
Size: 988 281 2010 2007 246 3079
Fragments arranged by size:
3079 2010 2007 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3673 748
Size: 2925 5686
HpaI GTT'AAC
Cuts at: 6201 6201
Size: 8611
KpnI G_GTAC'C
Cuts at: 2772 5936 2772
Size: 3164 5447
MfeI C'AATT_G
Cuts at: 2814 6210 2814
Size: 3396 5215
MluI A'CGCG_T
Cuts at: 8487 8487
Size: 8611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2966 3185 3265 7725 7909 1305
Size: 1661 219 80 4460 184 2007
Fragments arranged by size:
4460 2007 1661 219 184 80
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2682 2732 4593 10
Size: 54 1425 343 850 50 1861 4028
Fragments arranged by size:
4028 1861 1425 850 343 54 50
MslI CAynn'nnrTG
Cuts at: 518 1166 2978 4823 5392 5579 6569 6928 7087
Size: 648 1812 1845 569 187 990 359 159
Cuts at: 7087 518
Size: 2042
Fragments arranged by size:
2042 1845 1812 990 648 569 359 187
159
MspA1I CmG'CkG
Cuts at: 1606 2048 2276 3569 3908 5844 6027 6704 7852
Size: 442 228 1293 339 1936 183 677 1148
Cuts at: 7852 8097 1606
Size: 245 2120
Fragments arranged by size:
2120 1936 1293 1148 677 442 339 245
228 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7120
Fragments arranged by size:
7120 939 552
NdeI CA'TA_TG
Cuts at: 387 5389 387
Size: 5002 3609
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8611
NotI GC'GGCC_GC
Cuts at: 6058 6058
Size: 8611
NsiI A_TGCA'T
Cuts at: 5389 5389
Size: 8611
NspI r_CATG'y
Cuts at: 1132 1229 2317 2350 4553 5969 7514 1132
Size: 97 1088 33 2203 1416 1545 2229
Fragments arranged by size:
2229 2203 1545 1416 1088 97 33
PasI CC'CwG_GG
Cuts at: 1348 2997 4381 1348
Size: 1649 1384 5578
Fragments arranged by size:
5578 1649 1384
PciI A'CATG_T
Cuts at: 7510 7510
Size: 8611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2024 3296 3512 5856 1108
Size: 916 1272 216 2344 3863
Fragments arranged by size:
3863 2344 1272 916 216
PfoI T'CCnGG_A
Cuts at: 3378 3511 3378
Size: 133 8478
PmeI GTTT'AAAC
Cuts at: 5915 5915
Size: 8611
PmlI CAC'GTG
Cuts at: 5013 5013
Size: 8611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 6680 6712 8193 8225 2597
Size: 32 4051 32 1481 32 2983
Fragments arranged by size:
4051 2983 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2228 3227 4978 2228
Size: 999 1751 5861
Fragments arranged by size:
5861 1751 999
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8611
PsiI TTA'TAA
Cuts at: 6181 6181
Size: 8611
PspXI vC'TCGA_Gb
Cuts at: 5770 5770
Size: 8611
PstI C_TGCA'G
Cuts at: 830 2782 3307 3697 4612 5581 5963 830
Size: 1952 525 390 915 969 382 3478
Fragments arranged by size:
3478 1952 969 915 525 390 382
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8611
PvuII CAG'CTG
Cuts at: 3569 3908 5844 3569
Size: 339 1936 6336
Fragments arranged by size:
6336 1936 339
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 8611
SalI G'TCGA_C
Cuts at: 1936 5953 1936
Size: 4017 4594
SapI GCTCTTCn'nnn_
Cuts at: 2361 2430 4875 2361
Size: 69 2445 6097
Fragments arranged by size:
6097 2445 69
SbfI CC_TGCA'GG
Cuts at: 5963 5963
Size: 8611
ScaI AGT'ACT
Cuts at: 6859 8571 6859
Size: 1712 6899
SfcI C'TryA_G
Cuts at: 826 1045 2748 2778 3303 3693 4608 4648 5577
Size: 219 1703 30 525 390 915 40 929
Cuts at: 5577 5959 7094 7775 7966 826
Size: 382 1135 681 191 1471
Fragments arranged by size:
1703 1471 1135 929 915 681 525 390
382 219 191 40 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8611
SgrAI Cr'CCGG_yG
Cuts at: 1700 3196 1700
Size: 1496 7115
SmaI CCC'GGG
Cuts at: 1872 5938 1872
Size: 4066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2118 4544 5043 5499 5770 6715
Size: 197 926 175 2426 499 456 271 945
Cuts at: 6715 7616 7878 820
Size: 901 262 1553
Fragments arranged by size:
2426 1553 945 926 901 499 456 271
262 197 175
SnaBI TAC'GTA
Cuts at: 493 6402 493
Size: 5909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8611
SphI G_CATG'C
Cuts at: 1132 2317 5969 1132
Size: 1185 3652 3774
Fragments arranged by size:
3774 3652 1185
SspI AAT'ATT
Cuts at: 5 52 4040 5513 6535 5
Size: 47 3988 1473 1022 2081
Fragments arranged by size:
3988 2081 1473 1022 47
StuI AGG'CCT
Cuts at: 1517 2127 1517
Size: 610 8001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2224 2290 2601 2692 2876 3253
Size: 552 939 220 66 311 91 184 377
Cuts at: 3253 5230 6050 513
Size: 1977 820 3074
Fragments arranged by size:
3074 1977 939 820 552 377 311 220
184 91 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 6638 6655 6808 6993 7438 325
Size: 1255 1704 3354 17 153 185 445 1498
Fragments arranged by size:
3354 1704 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 2636 5343
Size: 274 80 33 51 1443 357 302 2707
Cuts at: 5343 5535 6857 8569 96
Size: 192 1322 1712 138
Fragments arranged by size:
2707 1712 1443 1322 357 302 274 192
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2923 2955 3771 3803 4997 5029 5851 5883 8571
Size: 32 816 32 1194 32 822 32 2688
Cuts at: 8571 8603 2923
Size: 32 2931
Fragments arranged by size:
2931 2688 1194 822 816 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8611
XbaI T'CTAG_A
Cuts at: 5947 5947
Size: 8611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3317 3317
Size: 8611
XhoI C'TCGA_G
Cuts at: 1943 5770 1943
Size: 3827 4784
XmaI C'CCGG_G
Cuts at: 1870 5936 1870
Size: 4066 4545
XmnI GAAnn'nnTTC
Cuts at: 5487 6740 5487
Size: 1253 7358
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6419 276
Size: 53 83 186 936 4885 2468
Fragments arranged by size:
4885 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PspOMI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI