Restriction map for
pFN21AB1499
(Circular) (Six-base) MAPSORT of: pFN21AB1499.seq Check: 4956 from: 1 to: 8668
pFN21AB1499 8668 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5000 5000
Size: 8668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6478 278
Size: 53 83 186 936 4942 2468
Fragments arranged by size:
4942 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3009 4667 6011 1937
Size: 1072 1658 1344 4594
Fragments arranged by size:
4594 1658 1344 1072
Acc65I G'GTAC_C
Cuts at: 4423 4855 5727 5989 4423
Size: 432 872 262 7102
Fragments arranged by size:
7102 872 432 262
AclI AA'CG_TT
Cuts at: 1563 6795 7168 1563
Size: 5232 373 3063
Fragments arranged by size:
5232 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4102 4728 6731 8115 8383 1632
Size: 2470 626 2003 1384 268 1917
Fragments arranged by size:
2470 2003 1917 1384 626 268
AfeI AGC'GCT
Cuts at: 3053 3053
Size: 8668
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8471
AflIII A'CryG_T
Cuts at: 1203 5069 5430 7567 8544 1203
Size: 3866 361 2137 977 1327
Fragments arranged by size:
3866 2137 1327 977 361
AgeI A'CCGG_T
Cuts at: 7499 7499
Size: 8668
AhdI GACnn_n'nnGTC
Cuts at: 7397 7397
Size: 8668
AleI CACnn'nnGTG
Cuts at: 1166 3410 1166
Size: 2244 6424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4898 1184
Size: 3714 4954
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4905 4937 4905
Size: 32 8636
AlwNI CAG_nnn'CTG
Cuts at: 2053 2128 2458 3389 3548 5166 6411 7983 2053
Size: 75 330 931 159 1618 1245 1572 2738
Fragments arranged by size:
2738 1618 1572 1245 931 330 159 75
ApaI G_GGCC'C
Cuts at: 2014 2804 2987 3159 3751 3782 2014
Size: 790 183 172 592 31 6900
Fragments arranged by size:
6900 790 592 183 172 31
ApaLI G'TGCA_C
Cuts at: 2835 5024 6725 7881 2835
Size: 2189 1701 1156 3622
Fragments arranged by size:
3622 2189 1701 1156
ApoI r'AATT_y
Cuts at: 1493 4441 4530 5959 5977 6203 6355 8464 1493
Size: 2948 89 1429 18 226 152 2109 1697
Fragments arranged by size:
2948 2109 1697 1429 226 152 89 18
AseI AT'TA_AT
Cuts at: 160 1032 5409 7222 160
Size: 872 4377 1813 1606
Fragments arranged by size:
4377 1813 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2505 2769 2920 2992 4264 4936
Size: 73 271 291 264 151 72 1272 672
Cuts at: 4936 5993 1870
Size: 1057 4545
Fragments arranged by size:
4545 1272 1057 672 291 271 264 151
73 72
AvrII C'CTAG_G
Cuts at: 3434 4771 3434
Size: 1337 7331
BamHI G'GATC_C
Cuts at: 4544 5998 6365 4544
Size: 1454 367 6847
Fragments arranged by size:
6847 1454 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2358 2461 2855 4423 4855
Size: 325 217 627 571 103 394 1568 432
Cuts at: 4855 5084 5180 5687 5727 5989 7444 618
Size: 229 96 507 40 262 1455 1842
Fragments arranged by size:
1842 1568 1455 627 571 507 432 394
325 262 229 217 103 96 40
BanII G_rGCy'C
Cuts at: 1391 2014 2115 2804 2987 3079 3159 3180 3623
Size: 623 101 689 183 92 80 21 443
Cuts at: 3623 3686 3696 3751 3782 4409 4586 4679 4937
Size: 63 10 55 31 627 177 93 258
Cuts at: 4937 5987 1391
Size: 1050 4072
Fragments arranged by size:
4072 1050 689 627 623 443 258 183
177 101 93 92 80 63 55 31
21 10
BbeI G_GCGC'C
Cuts at: 2362 2362
Size: 8668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2782 4276 4408 5048 928
Size: 971 883 1494 132 640 4548
Fragments arranged by size:
4548 1494 971 883 640 132
BbvCI CC'TCA_GC
Cuts at: 2532 3290 2532
Size: 758 7910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6859 6893 1903
Size: 34 4922 34 3678
Fragments arranged by size:
4922 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2667 4271 4594 6560 7770 2667
Size: 1604 323 1966 1210 3565
Fragments arranged by size:
3565 1966 1604 1210 323
BclI T'GATC_A
Cuts at: 1548 5107 1548
Size: 3559 5109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2543 2864
Size: 107 122 71 631 605 337 534 321
Cuts at: 2864 2971 5364 5696 7279 136
Size: 107 2393 332 1583 1525
Fragments arranged by size:
2393 1583 1525 631 605 534 337 332
321 122 107 107 71
BglII A'GATC_T
Cuts at: 3889 8663 3889
Size: 4774 3894
BlpI GC'TnA_GC
Cuts at: 2560 3347 4161 6085 2560
Size: 787 814 1924 5143
Fragments arranged by size:
5143 1924 814 787
Bme1580I G_kGCm'C
Cuts at: 1790 2014 2081 2466 2804 2839 2987 3159 3751
Size: 224 67 385 338 35 148 172 592
Cuts at: 3751 3782 5028 5639 5692 6729 7885 1790
Size: 31 1246 611 53 1037 1156 2573
Fragments arranged by size:
2573 1246 1156 1037 611 592 385 338
224 172 148 67 53 35 31
BmgBI CAC'GTC
Cuts at: 1318 3090 1318
Size: 1772 6896
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2662 3155 3342
Size: 416 53 83 399 24 1245 493 187
Cuts at: 3342 3777 4137 4509 4682 5950 6399 7357 442
Size: 435 360 372 173 1268 449 958 1753
Fragments arranged by size:
1753 1268 1245 958 493 449 435 416
399 372 360 187 173 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8668
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4846 5044 5692 4846
Size: 198 648 7822
Fragments arranged by size:
7822 648 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3194 3206 3678 3779 4153 4159 4431 4652
Size: 1955 12 472 101 374 6 272 221
Cuts at: 4652 4855 4937 7328 1239
Size: 203 82 2391 2579
Fragments arranged by size:
2579 2391 1955 472 374 272 221 203
101 82 12 6
Bpu10I CC'TnA_GC
Cuts at: 2532 3290 4146 2532
Size: 758 856 7054
Fragments arranged by size:
7054 856 758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4600 4666 6793 7658 7956 4600
Size: 66 2127 865 298 5312
Fragments arranged by size:
5312 2127 865 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3096 3104 4019 4127 4715 7331 882
Size: 617 1597 8 915 108 588 2616 2219
Fragments arranged by size:
2616 2219 1597 915 617 588 108 8
BsaAI yAC'GTr
Cuts at: 493 1204 2797 5433 6459 493
Size: 711 1593 2636 1026 2702
Fragments arranged by size:
2702 2636 1593 1026 711
BsaBI GATnn'nnATC
Cuts at: 8662 8662
Size: 8668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2359 2963 3060 4616
Size: 53 83 186 936 826 604 97 1556
Cuts at: 4616 6475 6857 7492 275
Size: 1859 382 635 1451
Fragments arranged by size:
1859 1556 1451 936 826 635 604 382
186 97 83 53
BsaWI w'CCGG_w
Cuts at: 3024 3490 5319 5389 7101 7499 7773 7920 8331
Size: 466 1829 70 1712 398 274 147 411
Cuts at: 8331 3024
Size: 3361
Fragments arranged by size:
3361 1829 1712 466 411 398 274 147
70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3128 3158 3557 3587 4157
Size: 30 165 30 1725 30 399 30 570
Cuts at: 4157 4187 4731 4761 4924 4954 1178
Size: 30 544 30 163 30 4892
Fragments arranged by size:
4892 1725 570 544 399 165 163 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3203 3278 4669 4973 4991 5033 1186
Size: 2017 75 1391 304 18 42 4821
Fragments arranged by size:
4821 2017 1391 304 75 42 18
BseYI C'CCAG_C
Cuts at: 2066 2128 2776 2986 3362 3528 3725 3845 5225
Size: 62 648 210 376 166 197 120 1380
Cuts at: 5225 5751 7871 8367 2066
Size: 526 2120 496 2367
Fragments arranged by size:
2367 2120 1380 648 526 496 376 210
197 166 120 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2867 4358 5800 1163
Size: 573 429 702 1491 1442 4031
Fragments arranged by size:
4031 1491 1442 702 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2695 6118 6879 7907 1540
Size: 275 186 694 3423 761 1028 2301
Fragments arranged by size:
3423 2301 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 2839 3079 3686 4167 5028 5646 6729 6814 7885
Size: 240 607 481 861 618 1083 85 1071
Cuts at: 7885 2839
Size: 3622
Fragments arranged by size:
3622 1083 1071 861 618 607 481 240
85
BsmI GAATG_Cn'
Cuts at: 3866 5011 5677 6179 6272 3866
Size: 1145 666 502 93 6262
Fragments arranged by size:
6262 1145 666 502 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2558 2558
Size: 8668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2081 2115 2466 2804 2839 2987
Size: 399 224 67 34 351 338 35 148
Cuts at: 2987 3079 3159 3180 3623 3686 3696 3751 3782
Size: 92 80 21 443 63 10 55 31
Cuts at: 3782 4167 4409 4586 4679 4937 5028 5639 5646
Size: 385 242 177 93 258 91 611 7
Cuts at: 5646 5692 5987 6729 6814 7885 1391
Size: 46 295 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 443 399 385 351
338 295 258 242 224 177 148 93
92 91 85 80 67 63 55 46
35 34 31 21 10 7
BspEI T'CCGG_A
Cuts at: 8331 8331
Size: 8668
BspHI T'CATG_A
Cuts at: 6557 8287 6557
Size: 1730 6938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5000 6023 844
Size: 4156 1023 3489
Fragments arranged by size:
4156 3489 1023
BsrBI CCG'CTC
Cuts at: 1394 1823 5361 6555 1394
Size: 429 3538 1194 3507
Fragments arranged by size:
3538 3507 1194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2568 5743 5791 7163 7337 65
Size: 1183 1320 3175 48 1372 174 1396
Fragments arranged by size:
3175 1396 1372 1320 1183 174 48
BsrFI r'CCGG_y
Cuts at: 1700 1810 2825 2970 3029 4507 4780 7312 7499
Size: 110 1015 145 59 1478 273 2532 187
Cuts at: 7499 1700
Size: 2869
Fragments arranged by size:
2869 2532 1478 1015 273 187 145 110
59
BsrGI T'GTAC_A
Cuts at: 96 4359 96
Size: 4263 4405
BssHII G'CGCG_C
Cuts at: 1925 2829 1925
Size: 904 7764
BssSI C'ACGA_G
Cuts at: 6728 7740 6728
Size: 1012 7656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 3389 794
Size: 1259 1336 6073
Fragments arranged by size:
6073 1336 1259
BstBI TT'CG_AA
Cuts at: 4911 7472 4911
Size: 2561 6107
BstEII G'GTnAC_C
Cuts at: 1184 3202 1184
Size: 2018 6650
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 3646 3696 2287
Size: 1359 50 7259
Fragments arranged by size:
7259 1359 50
BstYI r'GATC_y
Cuts at: 1972 3889 4544 5032 5998 6365 6750 6767 8219
Size: 1917 655 488 966 367 385 17 1452
Cuts at: 8219 8305 8317 8663 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1917 1452 966 655 488 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4253 4587 4840 4253
Size: 334 253 8081
Fragments arranged by size:
8081 334 253
BtgI C'CryG_G
Cuts at: 513 1065 2004 2198 3579 3714 3999 4181 4488
Size: 552 939 194 1381 135 285 182 307
Cuts at: 4488 4859 5272 513
Size: 371 413 3909
Fragments arranged by size:
3909 1381 939 552 413 371 307 285
194 182 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7929
Fragments arranged by size:
7929 683 56
BtsI GCAGTG_nn'
Cuts at: 2339 3387 3558 3610 5670 6184 6978 6998 2339
Size: 1048 171 52 2060 514 794 20 4009
Fragments arranged by size:
4009 2060 1048 794 514 171 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8633
DraI TTT'AAA
Cuts at: 5972 5972
Size: 8668
DraIII CAC_nnn'GTG
Cuts at: 3986 5761 3986
Size: 1775 6893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7675 809
Size: 507 6359 1802
Fragments arranged by size:
6359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2201 2289 2313
Size: 54 1425 50 275 18 371 88 24
Cuts at: 2313 2823 2931 3576 3607 3876 3903 5365 5763
Size: 510 108 645 31 269 27 1462 398
Cuts at: 5763 6115 7004 8633 8
Size: 352 889 1629 43
Fragments arranged by size:
1629 1462 1425 889 645 510 398 371
352 275 269 108 88 54 50 43
31 27 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 6115 1537
Size: 275 4303 4090
Fragments arranged by size:
4303 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3189 6598 1353
Size: 1836 3409 3423
Fragments arranged by size:
3423 3409 1836
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 7255 7629 7775 387
Size: 1064 138 585 5081 374 146 1280
Fragments arranged by size:
5081 1280 1064 585 374 146 138
EcoICRI GAG'CTC
Cuts at: 3077 3684 3077
Size: 607 8061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3194 3206 3678 3779 4102 4153 4159
Size: 393 1562 12 472 101 323 51 6
Cuts at: 4159 4431 4652 4728 4855 4937 6731 7328 8115
Size: 272 221 76 127 82 1794 597 787
Cuts at: 8115 8383 1239
Size: 268 1524
Fragments arranged by size:
1794 1562 1524 787 597 472 393 323
272 268 221 127 101 82 76 51
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3188 4634 3188
Size: 1446 7222
EcoO109I rG'GnC_Cy
Cuts at: 2010 2011 2455 2902 2983 3155 3156 3307 3747
Size: 1 444 447 81 172 1 151 440
Cuts at: 3747 3748 3778 3779 4342 4472 4864 4921 4995
Size: 1 30 1 563 130 392 57 74
Cuts at: 4995 5802 2010
Size: 807 4876
Fragments arranged by size:
4876 807 563 447 444 440 392 172
151 130 81 74 57 30 1 1
1 1
EcoRI G'AATT_C
Cuts at: 5977 6355 5977
Size: 378 8290
EcoRV GAT'ATC
Cuts at: 2089 2089
Size: 8668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4323 4323
Size: 8668
FspI TGC'GCA
Cuts at: 3002 7174 3002
Size: 4172 4496
FspAI rTGC'GCAy
Cuts at: 3002 3002
Size: 8668
HaeII r_GCGC'y
Cuts at: 1678 2362 2977 3055 3451 3472 3528 5587 7815
Size: 684 615 78 396 21 56 2059 2228
Cuts at: 7815 1678
Size: 2531
Fragments arranged by size:
2531 2228 2059 684 615 396 78 56
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3101 3128 3133 3160 7309
Size: 32 135 32 1529 27 5 27 4149
Cuts at: 7309 7341 7383 7415 1373
Size: 32 42 32 2626
Fragments arranged by size:
4149 2626 1529 135 42 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4153 6012 6258 669
Size: 988 281 2215 1859 246 3079
Fragments arranged by size:
3079 2215 1859 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8668
HpaI GTT'AAC
Cuts at: 6258 6258
Size: 8668
KasI G'GCGC_C
Cuts at: 2358 2358
Size: 8668
KpnI G_GTAC'C
Cuts at: 4427 4859 5731 5993 4427
Size: 432 872 262 7102
Fragments arranged by size:
7102 872 432 262
MfeI C'AATT_G
Cuts at: 6267 6267
Size: 8668
MluI A'CGCG_T
Cuts at: 8544 8544
Size: 8668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4630 7782 7966 1305
Size: 3325 3152 184 2007
Fragments arranged by size:
3325 3152 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 2315 3578 3609 3878
Size: 54 1425 343 459 24 1263 31 269
Cuts at: 3878 3905 5765 10
Size: 27 1860 2913
Fragments arranged by size:
2913 1860 1425 1263 459 343 269 54
31 27 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3410 3593 6626 6985 7144 518
Size: 648 1119 1125 183 3033 359 159 2042
Fragments arranged by size:
3033 2042 1125 1119 648 359 183 159
MspA1I CmG'CkG
Cuts at: 1606 2421 2881 5274 6084 6761 7909 8154 1606
Size: 815 460 2393 810 677 1148 245 2120
Fragments arranged by size:
2393 2120 1148 815 810 677 460 245
NaeI GCC'GGC
Cuts at: 2827 2972 4509 2827
Size: 145 1537 6986
Fragments arranged by size:
6986 1537 145
NarI GG'CG_CC
Cuts at: 2359 2359
Size: 8668
NcoI C'CATG_G
Cuts at: 513 1065 2004 3579 3714 4181 4488 513
Size: 552 939 1575 135 467 307 4693
Fragments arranged by size:
4693 1575 939 552 467 307 135
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8668
NgoMIV G'CCGG_C
Cuts at: 2825 2970 4507 2825
Size: 145 1537 6986
Fragments arranged by size:
6986 1537 145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8668
NotI GC'GGCC_GC
Cuts at: 6115 6115
Size: 8668
NspI r_CATG'y
Cuts at: 1132 1229 2122 5073 6026 7571 1132
Size: 97 893 2951 953 1545 2229
Fragments arranged by size:
2951 2229 1545 953 893 97
PasI CC'CwG_GG
Cuts at: 1348 3302 3624 3858 4107 4399 4726 4894 1348
Size: 1954 322 234 249 292 327 168 5122
Fragments arranged by size:
5122 1954 327 322 292 249 234 168
PciI A'CATG_T
Cuts at: 5069 7567 5069
Size: 2498 6170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2322 3122 3927 4182 1108
Size: 1214 800 805 255 5594
Fragments arranged by size:
5594 1214 805 800 255
PfoI T'CCnGG_A
Cuts at: 3216 3756 3216
Size: 540 8128
PmeI GTTT'AAAC
Cuts at: 5972 5972
Size: 8668
PmlI CAC'GTG
Cuts at: 5433 5433
Size: 8668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6737 6769 8250 8282 6737
Size: 32 1481 32 7123
Fragments arranged by size:
7123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2455 3307 4342 4472 4864 4921 4995 2455
Size: 852 1035 130 392 57 74 6128
Fragments arranged by size:
6128 1035 852 392 130 74 57
PshAI GACnn'nnGTC
Cuts at: 1613 2335 5378 1613
Size: 722 3043 4903
Fragments arranged by size:
4903 3043 722
PsiI TTA'TAA
Cuts at: 6238 6238
Size: 8668
PspOMI G'GGCC_C
Cuts at: 2010 2800 2983 3155 3747 3778 2010
Size: 790 183 172 592 31 6900
Fragments arranged by size:
6900 790 592 183 172 31
PstI C_TGCA'G
Cuts at: 830 2049 2196 3394 3553 4024 4810 5162 6020
Size: 1219 147 1198 159 471 786 352 858
Cuts at: 6020 830
Size: 3478
Fragments arranged by size:
3478 1219 1198 858 786 471 352 159
147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8668
PvuII CAG'CTG
Cuts at: 2881 2881
Size: 8668
RsrII CG'GwC_CG
Cuts at: 3027 3027
Size: 8668
SacI G_AGCT'C
Cuts at: 3079 3686 3079
Size: 607 8061
SacII CC_GC'GG
Cuts at: 5275 5275
Size: 8668
SalI G'TCGA_C
Cuts at: 1936 6010 1936
Size: 4074 4594
SanDI GG'GwC_CC
Cuts at: 3307 4864 4995 3307
Size: 1557 131 6980
Fragments arranged by size:
6980 1557 131
SbfI CC_TGCA'GG
Cuts at: 6020 6020
Size: 8668
ScaI AGT'ACT
Cuts at: 6916 8628 6916
Size: 1712 6956
SexAI A'CCwGG_T
Cuts at: 2457 2457
Size: 8668
SfcI C'TryA_G
Cuts at: 826 1045 2045 2192 2588 3390 3549 4020 4209
Size: 219 1000 147 396 802 159 471 189
Cuts at: 4209 4326 4461 4806 5098 5158 6016 7151 7832
Size: 117 135 345 292 60 858 1135 681
Cuts at: 7832 8023 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1000 858 802 681 471 396
345 292 219 191 189 159 147 135
117 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2543 2543
Size: 8668
SfoI GGC'GCC
Cuts at: 2360 2360
Size: 8668
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
SgrAI Cr'CCGG_yG
Cuts at: 1700 2970 1700
Size: 1270 7398
SmaI CCC'GGG
Cuts at: 1872 2507 2771 2922 2994 4266 4938 5995 1872
Size: 635 264 151 72 1272 672 1057 4545
Fragments arranged by size:
4545 1272 1057 672 635 264 151 72
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4579 4681 6772 7673 7935 820
Size: 197 926 2636 102 2091 901 262 1553
Fragments arranged by size:
2636 2091 1553 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 6459 493
Size: 5966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8668
SphI G_CATG'C
Cuts at: 1132 6026 1132
Size: 4894 3774
SspI AAT'ATT
Cuts at: 5 52 6592 5
Size: 47 6540 2081
Fragments arranged by size:
6540 2081 47
StuI AGG'CCT
Cuts at: 1517 2546 1517
Size: 1029 7639
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2241 2292 2466 3434 3579
Size: 552 939 24 213 51 174 968 145
Cuts at: 3579 3714 3822 4029 4181 4451 4488 4771 6107
Size: 135 108 207 152 270 37 283 1336
Cuts at: 6107 513
Size: 3074
Fragments arranged by size:
3074 1336 968 939 552 283 270 213
207 174 152 145 135 108 51 37
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 3739 4713 5943 6695 6712 6865
Size: 1255 1101 1058 974 1230 752 17 153
Cuts at: 6865 7050 7495 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1230 1101 1058 974 752 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4359 5703 6914
Size: 274 80 33 51 1443 2382 1344 1211
Cuts at: 6914 8626 96
Size: 1712 138
Fragments arranged by size:
2382 1712 1443 1344 1211 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4157 4189 4464 4468 4496 4500 4536 4568 4731
Size: 32 275 4 28 4 36 32 163
Cuts at: 4731 4763 4924 4956 5879 5911 8628 8660 4157
Size: 32 161 32 923 32 2717 32 4165
Fragments arranged by size:
4165 2717 923 275 163 161 36 32
32 32 32 32 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 1316 2254 2300 1316
Size: 938 46 7684
Fragments arranged by size:
7684 938 46
XbaI T'CTAG_A
Cuts at: 6004 6004
Size: 8668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2675 3810 4669 5937 2380
Size: 295 1135 859 1268 5111
Fragments arranged by size:
5111 1268 1135 859 295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8668
XmaI C'CCGG_G
Cuts at: 1870 2505 2769 2920 2992 4264 4936 5993 1870
Size: 635 264 151 72 1272 672 1057 4545
Fragments arranged by size:
4545 1272 1057 672 635 264 151 72
XmnI GAAnn'nnTTC
Cuts at: 6797 6797
Size: 8668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6476 276
Size: 53 83 186 936 4942 2468
Fragments arranged by size:
4942 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I FseI NruI NsiI
PacI PspXI PsrI SapI SrfI SwaI