Restriction map for
pFN21AB1488
(Circular) (Six-base) MAPSORT of: pFN21AB1488.seq Check: 4456 from: 1 to: 9937
pFN21AB1488 9937 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4515 4651 4515
Size: 136 9801
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6468 7747 278
Size: 53 83 186 936 4932 1279 2468
Fragments arranged by size:
4932 2468 1279 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7280 1937
Size: 5343 4594
Acc65I G'GTAC_C
Cuts at: 2475 7258 2475
Size: 4783 5154
AclI AA'CG_TT
Cuts at: 1563 8064 8437 1563
Size: 6501 373 3063
Fragments arranged by size:
6501 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3273 3387 3920 4757 6158 6780 8000 9384
Size: 1641 114 533 837 1401 622 1220 1384
Cuts at: 9384 9652 1632
Size: 268 1917
Fragments arranged by size:
1917 1641 1401 1384 1220 837 622 533
268 114
AfeI AGC'GCT
Cuts at: 4106 4106
Size: 9937
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9740
AflIII A'CryG_T
Cuts at: 1203 4127 4608 8836 9813 1203
Size: 2924 481 4228 977 1327
Fragments arranged by size:
4228 2924 1327 977 481
AgeI A'CCGG_T
Cuts at: 8768 8768
Size: 9937
AhdI GACnn_n'nnGTC
Cuts at: 2365 8666 2365
Size: 6301 3636
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9937
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 9905
AlwNI CAG_nnn'CTG
Cuts at: 3010 3200 3350 4666 5232 5705 7108 7680 9252
Size: 190 150 1316 566 473 1403 572 1572
Cuts at: 9252 3010
Size: 3695
Fragments arranged by size:
3695 1572 1403 1316 572 566 473 190
150
ApaI G_GGCC'C
Cuts at: 3151 6564 3151
Size: 3413 6524
ApaLI G'TGCA_C
Cuts at: 6401 7994 9150 6401
Size: 1593 1156 7188
Fragments arranged by size:
7188 1593 1156
ApoI r'AATT_y
Cuts at: 1493 4763 7246 7472 7624 9733 1493
Size: 3270 2483 226 152 2109 1697
Fragments arranged by size:
3270 2483 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4065 8491 160
Size: 872 3033 4426 1606
Fragments arranged by size:
4426 3033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9937
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2509 3413 3537 4749 5025 5120
Size: 73 202 364 904 124 1212 276 95
Cuts at: 5120 6600 6689 7088 7262 1870
Size: 1480 89 399 174 4545
Fragments arranged by size:
4545 1480 1212 904 399 364 276 202
174 124 95 89 73
BamHI G'GATC_C
Cuts at: 5169 7267 7634 5169
Size: 2098 367 7472
Fragments arranged by size:
7472 2098 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2269 2421 2475 2767 3616
Size: 325 217 627 482 152 54 292 849
Cuts at: 3616 3734 4669 4708 5570 5617 6367 6718 7227
Size: 118 935 39 862 47 750 351 509
Cuts at: 7227 7258 8713 618
Size: 31 1455 1842
Fragments arranged by size:
1842 1455 935 862 849 750 627 509
482 351 325 292 217 152 118 54
47 39 31
BanII G_rGCy'C
Cuts at: 1391 2862 3075 3151 3570 3723 4197 4276 4682
Size: 1471 213 76 419 153 474 79 406
Cuts at: 4682 5565 5699 6564 7256 1391
Size: 883 134 865 692 4072
Fragments arranged by size:
4072 1471 883 865 692 474 419 406
213 153 134 79 76
BbeI G_GCGC'C
Cuts at: 5621 6371 5621
Size: 750 9187
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2888 3225 4028 5461 6912 928
Size: 971 989 337 803 1433 1451 3953
Fragments arranged by size:
3953 1451 1433 989 971 803 337
BbvCI CC'TCA_GC
Cuts at: 6777 6777
Size: 9937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6439 6473 7002 7036 8128 8162 1903
Size: 34 4502 34 529 34 1092 34 3678
Fragments arranged by size:
4502 3678 1092 529 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7829 9039 7829
Size: 1210 8727
BclI T'GATC_A
Cuts at: 1548 7056 1548
Size: 5508 4429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3800 4260 5591
Size: 107 122 71 631 605 2128 460 1331
Cuts at: 5591 6643 8548 136
Size: 1052 1905 1525
Fragments arranged by size:
2128 1905 1525 1331 1052 631 605 460
122 107 71
BglII A'GATC_T
Cuts at: 2646 9932 2646
Size: 7286 2651
BlpI GC'TnA_GC
Cuts at: 3722 7354 3722
Size: 3632 6305
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2274 2424 3151 3934 4713 5575 6405
Size: 355 129 150 727 783 779 862 830
Cuts at: 6405 6564 6721 6791 7189 7998 9154 1790
Size: 159 157 70 398 809 1156 2573
Fragments arranged by size:
2573 1156 862 830 809 783 779 727
398 355 159 157 150 129 70
BmgBI CAC'GTC
Cuts at: 1318 4130 6223 6568 1318
Size: 2812 2093 345 4687
Fragments arranged by size:
4687 2812 2093 345
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2686 3656 4469
Size: 416 53 83 399 24 1269 970 813
Cuts at: 4469 4610 5701 7096 7668 8626 442
Size: 141 1091 1395 572 958 1753
Fragments arranged by size:
1753 1395 1269 1091 970 958 813 572
416 399 141 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9937
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5040 7159 5040
Size: 2119 7818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2249 2921 2987 2993 3030 3737 4298
Size: 878 132 672 66 6 37 707 561
Cuts at: 4298 4375 4571 5697 6086 6527 6830 7190 8597
Size: 77 196 1126 389 441 303 360 1407
Cuts at: 8597 1239
Size: 2579
Fragments arranged by size:
2579 1407 1126 878 707 672 561 441
389 360 303 196 132 77 66 37
6
Bpu10I CC'TnA_GC
Cuts at: 2231 4436 6777 2231
Size: 2205 2341 5391
Fragments arranged by size:
5391 2341 2205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5035 8062 8927 9225 5035
Size: 3027 865 298 5747
Fragments arranged by size:
5747 3027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3595 3648 3969 5117 5987 6545 6929
Size: 617 2096 53 321 1148 870 558 384
Cuts at: 6929 8600 882
Size: 1671 2219
Fragments arranged by size:
2219 2096 1671 1148 870 617 558 384
321 53
BsaAI yAC'GTr
Cuts at: 493 1204 4419 4609 6538 7728 493
Size: 711 3215 190 1929 1190 2702
Fragments arranged by size:
3215 2702 1929 1190 711 190
BsaBI GATnn'nnATC
Cuts at: 9931 9931
Size: 9937
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2967 5618 6368 6465
Size: 53 83 186 936 1434 2651 750 97
Cuts at: 6465 7744 8126 8761 275
Size: 1279 382 635 1451
Fragments arranged by size:
2651 1451 1434 1279 936 750 635 382
186 97 83 53
BsaWI w'CCGG_w
Cuts at: 3747 8370 8768 9042 9189 9600 3747
Size: 4623 398 274 147 411 4084
Fragments arranged by size:
4623 4084 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4383 4413 4613 4643 4767
Size: 30 165 30 2980 30 200 30 124
Cuts at: 4767 4797 5101 5131 5725 5755 6065 6095 6377
Size: 30 304 30 594 30 310 30 282
Cuts at: 6377 6407 1178
Size: 30 4708
Fragments arranged by size:
4708 2980 594 310 304 282 200 165
124 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2246 2300 2398 3077 3441 4307 4844 5733
Size: 1060 54 98 679 364 866 537 889
Cuts at: 5733 5987 6083 6086 6398 7148 1186
Size: 254 96 3 312 750 3975
Fragments arranged by size:
3975 1060 889 866 750 679 537 364
312 254 98 96 54 3
BseYI C'CCAG_C
Cuts at: 3193 3695 3805 4637 4656 5331 5558 5776 7188
Size: 502 110 832 19 675 227 218 1412
Cuts at: 7188 9140 9636 3193
Size: 1952 496 3494
Fragments arranged by size:
3494 1952 1412 832 675 502 496 227
218 110 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2852 4193 4493 5505 5798 6647
Size: 573 411 705 1341 300 1012 293 849
Cuts at: 6647 6671 6941 1163
Size: 24 270 4159
Fragments arranged by size:
4159 1341 1012 849 705 573 411 300
293 270 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3437 7387 8148 9176 1540
Size: 275 186 1436 3950 761 1028 2301
Fragments arranged by size:
3950 2301 1436 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2516 3075 3723 5111 6405 7166 7998 8083 9154
Size: 559 648 1388 1294 761 832 85 1071
Cuts at: 9154 2516
Size: 3299
Fragments arranged by size:
3299 1388 1294 1071 832 761 648 559
85
BsmI GAATG_Cn'
Cuts at: 3204 3300 7448 7541 3204
Size: 96 4148 93 5600
Fragments arranged by size:
5600 4148 96 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2274 2424 2516 2862 3075 3151
Size: 399 355 129 150 92 346 213 76
Cuts at: 3151 3570 3723 3934 4197 4276 4682 4713 5111
Size: 419 153 211 263 79 406 31 398
Cuts at: 5111 5565 5575 5699 6405 6564 6721 6791 7166
Size: 454 10 124 706 159 157 70 375
Cuts at: 7166 7189 7256 7998 8083 9154 1391
Size: 23 67 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 454 419 406 399
398 375 355 346 263 213 211 159
157 153 150 129 124 92 85 79
76 70 67 31 23 10
BspEI T'CCGG_A
Cuts at: 9600 9600
Size: 9937
BspHI T'CATG_A
Cuts at: 3555 7826 9556 3555
Size: 4271 1730 3936
Fragments arranged by size:
4271 3936 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 4515 4651 5269 5637 6191 7116 7292
Size: 1562 2109 136 618 368 554 925 176
Cuts at: 7292 844
Size: 3489
Fragments arranged by size:
3489 2109 1562 925 618 554 368 176
136
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 3433 6814 7824 1394
Size: 429 1465 145 3381 1010 3507
Fragments arranged by size:
3507 3381 1465 1010 429 145
BsrDI GCAATG_nn'
Cuts at: 65 1248 5413 5667 5725 8432 8606 65
Size: 1183 4165 254 58 2707 174 1396
Fragments arranged by size:
4165 2707 1396 1183 254 174 58
BsrFI r'CCGG_y
Cuts at: 1700 1810 6637 6655 8581 8768 1700
Size: 110 4827 18 1926 187 2869
Fragments arranged by size:
4827 2869 1926 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 2577 96
Size: 2481 7456
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9937
BssSI C'ACGA_G
Cuts at: 3453 5828 7997 9009 3453
Size: 2375 2169 1012 4381
Fragments arranged by size:
4381 2375 2169 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5821 6634 7184 794
Size: 5027 813 550 3547
Fragments arranged by size:
5027 3547 813 550
BstBI TT'CG_AA
Cuts at: 4761 8741 4761
Size: 3980 5957
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9937
BstXI CCAn_nnnn'nTGG
Cuts at: 2640 4347 5675 5784 6379 7214 2640
Size: 1707 1328 109 595 835 5363
Fragments arranged by size:
5363 1707 1328 835 595 109
BstYI r'GATC_y
Cuts at: 1972 2646 3112 4281 4425 4954 5143 5169 7267
Size: 674 466 1169 144 529 189 26 2098
Cuts at: 7267 7634 8019 8036 9488 9574 9586 9932 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2098 1977 1452 1169 674 529 466 385
367 346 189 144 86 26 17 12
Bsu36I CC'TnA_GG
Cuts at: 2322 2322
Size: 9937
BtgI C'CryG_G
Cuts at: 513 1065 2348 3225 3489 3649 4429 4713 5139
Size: 552 1283 877 264 160 780 284 426
Cuts at: 5139 513
Size: 5311
Fragments arranged by size:
5311 1283 877 780 552 426 284 264
160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5428 6442 487
Size: 683 56 4202 1014 3982
Fragments arranged by size:
4202 3982 1014 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 5108 5111 7453 8247 8267 3502
Size: 1606 3 2342 794 20 5172
Fragments arranged by size:
5172 2342 1606 794 20 3
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9902
DraI TTT'AAA
Cuts at: 7241 7241
Size: 9937
DraIII CAC_nnn'GTG
Cuts at: 3139 3664 5140 6414 6750 3139
Size: 525 1476 1274 336 6326
Fragments arranged by size:
6326 1476 1274 525 336
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4008 8944 809
Size: 507 2692 4936 1802
Fragments arranged by size:
4936 2692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2461 3289 3434
Size: 54 1425 50 275 18 631 828 145
Cuts at: 3434 3666 4467 5463 6453 6657 6741 7384 8273
Size: 232 801 996 990 204 84 643 889
Cuts at: 8273 9902 8
Size: 1629 43
Fragments arranged by size:
1629 1425 996 990 889 828 801 643
631 275 232 204 145 84 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3434 7384 1537
Size: 275 1622 3950 4090
Fragments arranged by size:
4090 3950 1622 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 2408 4232 7867 1353
Size: 929 126 1824 3635 3423
Fragments arranged by size:
3635 3423 1824 929 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4505 4523 4659 5204 6439 6914
Size: 1064 138 2916 18 136 545 1235 475
Cuts at: 6914 8524 8898 9044 387
Size: 1610 374 146 1280
Fragments arranged by size:
2916 1610 1280 1235 1064 545 475 374
146 138 136 18
EcoICRI GAG'CTC
Cuts at: 3073 3721 3073
Size: 648 9289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2249 2921 2987 2993 3030 3273
Size: 393 485 132 672 66 6 37 243
Cuts at: 3273 3387 3737 3920 4298 4375 4571 4757 5697
Size: 114 350 183 378 77 196 186 940
Cuts at: 5697 6086 6158 6527 6780 6830 7190 8000 8597
Size: 389 72 369 253 50 360 810 597
Cuts at: 8597 9384 9652 1239
Size: 787 268 1524
Fragments arranged by size:
1524 940 810 787 672 597 485 393
389 378 369 360 350 268 253 243
196 186 183 132 114 77 72 66
50 37 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2867 2948 3161 3405 4289 6068 2867
Size: 81 213 244 884 1779 6736
Fragments arranged by size:
6736 1779 884 244 213 81
EcoO109I rG'GnC_Cy
Cuts at: 2107 2318 2637 3147 3148 3410 3576 3699 4297
Size: 211 319 510 1 262 166 123 598
Cuts at: 4297 4755 4983 5257 5359 5773 6142 6190 6560
Size: 458 228 274 102 414 369 48 370
Cuts at: 6560 6561 2107
Size: 1 5483
Fragments arranged by size:
5483 598 510 458 414 370 369 319
274 262 228 211 166 123 102 48
1 1
EcoRI G'AATT_C
Cuts at: 4763 7246 7624 4763
Size: 2483 378 7076
Fragments arranged by size:
7076 2483 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2905 3790 2239
Size: 666 885 8386
Fragments arranged by size:
8386 885 666
FspI TGC'GCA
Cuts at: 4232 4328 6998 8443 4232
Size: 96 2670 1445 5726
Fragments arranged by size:
5726 2670 1445 96
HaeII r_GCGC'y
Cuts at: 1678 3806 4108 5621 6371 9084 1678
Size: 2128 302 1513 750 2713 2531
Fragments arranged by size:
2713 2531 2128 1513 750 302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 3062 3094 3531
Size: 32 135 32 779 32 679 32 437
Cuts at: 3531 3563 3672 3704 4769 4801 5924 5956 6377
Size: 32 109 32 1065 32 1123 32 421
Cuts at: 6377 6409 7048 7080 8578 8610 8652 8684 1373
Size: 32 639 32 1498 32 42 32 2626
Fragments arranged by size:
2626 1498 1123 1065 779 679 639 437
421 135 109 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7281 7527 669
Size: 988 281 5343 246 3079
Fragments arranged by size:
5343 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9937
HpaI GTT'AAC
Cuts at: 7527 7527
Size: 9937
KasI G'GCGC_C
Cuts at: 5617 6367 5617
Size: 750 9187
KpnI G_GTAC'C
Cuts at: 2479 7262 2479
Size: 4783 5154
MfeI C'AATT_G
Cuts at: 7536 7536
Size: 9937
MluI A'CGCG_T
Cuts at: 9813 9813
Size: 9937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 4291 5087 5781 6323 9051 9235 1305
Size: 1398 1588 796 694 542 2728 184 2007
Fragments arranged by size:
2728 2007 1588 1398 796 694 542 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2463 3668 4469 6455 6743
Size: 54 1425 343 631 1205 801 1986 288
Cuts at: 6743 10
Size: 3204
Fragments arranged by size:
3204 1986 1425 1205 801 631 343 288
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2781 4322 5660 7895 8254 8413
Size: 648 1365 250 1541 1338 2235 359 159
Cuts at: 8413 518
Size: 2042
Fragments arranged by size:
2235 2042 1541 1365 1338 648 359 250
159
MspA1I CmG'CkG
Cuts at: 1606 2332 2467 3953 4159 4549 4727 5582 6040
Size: 726 135 1486 206 390 178 855 458
Cuts at: 6040 6331 6983 7174 7353 8030 9178 9423 1606
Size: 291 652 191 179 677 1148 245 2120
Fragments arranged by size:
2120 1486 1148 855 726 677 652 458
390 291 245 206 191 179 178 135
NaeI GCC'GGC
Cuts at: 6657 6657
Size: 9937
NarI GG'CG_CC
Cuts at: 5618 6368 5618
Size: 750 9187
NcoI C'CATG_G
Cuts at: 513 1065 2348 3649 4429 513
Size: 552 1283 1301 780 6021
Fragments arranged by size:
6021 1301 1283 780 552
NdeI CA'TA_TG
Cuts at: 387 2845 387
Size: 2458 7479
NgoMIV G'CCGG_C
Cuts at: 6655 6655
Size: 9937
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9937
NotI GC'GGCC_GC
Cuts at: 7384 7384
Size: 9937
NspI r_CATG'y
Cuts at: 1132 1229 2584 5167 5389 7295 8840 1132
Size: 97 1355 2583 222 1906 1545 2229
Fragments arranged by size:
2583 2229 1906 1545 1355 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9937
PciI A'CATG_T
Cuts at: 8836 8836
Size: 9937
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 3200 3613 3664 5140 1108
Size: 1227 865 413 51 1476 5905
Fragments arranged by size:
5905 1476 1227 865 413 51
PfoI T'CCnGG_A
Cuts at: 2133 2598 2961 3258 5355 5472 6162 7206 2133
Size: 465 363 297 2097 117 690 1044 4864
Fragments arranged by size:
4864 2097 1044 690 465 363 297 117
PmeI GTTT'AAAC
Cuts at: 7241 7241
Size: 9937
PmlI CAC'GTG
Cuts at: 4419 4609 4419
Size: 190 9747
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8006 8038 9519 9551 8006
Size: 32 1481 32 8392
Fragments arranged by size:
8392 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 2637 3576 4297 4755 5257 5359 6142 6190
Size: 530 939 721 458 502 102 783 48
Cuts at: 6190 2107
Size: 5854
Fragments arranged by size:
5854 939 783 721 530 502 458 102
48
PshAI GACnn'nnGTC
Cuts at: 1613 3587 5364 1613
Size: 1974 1777 6186
Fragments arranged by size:
6186 1974 1777
PsiI TTA'TAA
Cuts at: 7507 7507
Size: 9937
PspOMI G'GGCC_C
Cuts at: 3147 6560 3147
Size: 3413 6524
PspXI vC'TCGA_Gb
Cuts at: 4749 5025 7088 4749
Size: 276 2063 7598
Fragments arranged by size:
7598 2063 276
PstI C_TGCA'G
Cuts at: 830 2061 3031 3165 3339 3355 3838 4290 4800
Size: 1231 970 134 174 16 483 452 510
Cuts at: 4800 5307 6039 6081 6312 6330 6825 7289 830
Size: 507 732 42 231 18 495 464 3478
Fragments arranged by size:
3478 1231 970 732 510 507 495 483
464 452 231 174 134 42 18 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9937
PvuII CAG'CTG
Cuts at: 2332 4549 5582 6040 6331 2332
Size: 2217 1033 458 291 5938
Fragments arranged by size:
5938 2217 1033 458 291
SacI G_AGCT'C
Cuts at: 3075 3723 3075
Size: 648 9289
SalI G'TCGA_C
Cuts at: 1936 7279 1936
Size: 5343 4594
SanDI GG'GwC_CC
Cuts at: 2107 2107
Size: 9937
SapI GCTCTTCn'nnn_
Cuts at: 2282 2408 4232 2282
Size: 126 1824 7987
Fragments arranged by size:
7987 1824 126
SbfI CC_TGCA'GG
Cuts at: 4290 7289 4290
Size: 2999 6938
ScaI AGT'ACT
Cuts at: 8185 9897 8185
Size: 1712 8225
SfcI C'TryA_G
Cuts at: 826 1045 2057 3027 3161 3335 3351 3834 4286
Size: 219 1012 970 134 174 16 483 452
Cuts at: 4286 4796 5303 6035 6077 6194 6308 6326 6821
Size: 510 507 732 42 117 114 18 495
Cuts at: 6821 6838 7285 8420 9101 9292 826
Size: 17 447 1135 681 191 1471
Fragments arranged by size:
1471 1135 1012 970 732 681 510 507
495 483 452 447 219 191 174 134
117 114 42 18 17 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 4260 4260
Size: 9937
SfoI GGC'GCC
Cuts at: 5619 6369 5619
Size: 750 9187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9937
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9937
SmaI CCC'GGG
Cuts at: 1872 3415 6602 7264 1872
Size: 1543 3187 662 4545
Fragments arranged by size:
4545 3187 1543 662
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4749 5014 5025 7088 8041 8942
Size: 197 926 2806 265 11 2063 953 901
Cuts at: 8942 9204 820
Size: 262 1553
Fragments arranged by size:
2806 2063 1553 953 926 901 265 262
197 11
SnaBI TAC'GTA
Cuts at: 493 7728 493
Size: 7235 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9937
SphI G_CATG'C
Cuts at: 1132 5389 7295 1132
Size: 4257 1906 3774
Fragments arranged by size:
4257 3774 1906
SspI AAT'ATT
Cuts at: 5 52 4386 7052 7861 5
Size: 47 4334 2666 809 2081
Fragments arranged by size:
4334 2666 2081 809 47
StuI AGG'CCT
Cuts at: 1517 3167 4458 1517
Size: 1650 1291 6996
Fragments arranged by size:
6996 1650 1291
StyI C'CwwG_G
Cuts at: 513 1065 2348 3108 3168 3649 3813 4014 4429
Size: 552 1283 760 60 481 164 201 415
Cuts at: 4429 4788 4854 4875 5127 5613 5890 6087 6285
Size: 359 66 21 252 486 277 197 198
Cuts at: 6285 6372 6966 7376 513
Size: 87 594 410 3074
Fragments arranged by size:
3074 1283 760 594 552 486 481 415
410 359 277 252 201 198 197 164
87 66 60 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 3577 3675 5558 6569 6577 6962
Size: 1255 1125 872 98 1883 1011 8 385
Cuts at: 6962 7964 7981 8134 8319 8764 325
Size: 1002 17 153 185 445 1498
Fragments arranged by size:
1883 1498 1255 1125 1011 1002 872 445
385 185 153 98 17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 5688 6228
Size: 274 80 33 51 1443 600 3111 540
Cuts at: 6228 8183 9895 96
Size: 1955 1712 138
Fragments arranged by size:
3111 1955 1712 1443 600 540 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2142 2174 4383 4415 4613 4645 4767 4799 5101
Size: 32 2209 32 198 32 122 32 302
Cuts at: 5101 5133 5725 5757 9897 9929 2142
Size: 32 592 32 4140 32 2150
Fragments arranged by size:
4140 2209 2150 592 302 198 122 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6587 1316
Size: 5271 4666
XbaI T'CTAG_A
Cuts at: 7273 7273
Size: 9937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 3385 5053 5620 2530
Size: 855 1668 567 6847
Fragments arranged by size:
6847 1668 855 567
XhoI C'TCGA_G
Cuts at: 1943 4749 5025 7088 1943
Size: 2806 276 2063 4792
Fragments arranged by size:
4792 2806 2063 276
XmaI C'CCGG_G
Cuts at: 1870 3413 6600 7262 1870
Size: 1543 3187 662 4545
Fragments arranged by size:
4545 3187 1543 662
XmnI GAAnn'nnTTC
Cuts at: 2885 6017 8066 2885
Size: 3132 2049 4756
Fragments arranged by size:
4756 3132 2049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6466 7745 276
Size: 53 83 186 936 4932 1279 2468
Fragments arranged by size:
4932 2468 1279 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PsrI RsrII SacII
SexAI SrfI SwaI