Restriction map for
pFN21AB1434
(Circular) (Six-base) MAPSORT of: pFN21AB1434.seq Check: 8202 from: 1 to: 11011
pFN21AB1434 11011 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3717 5250 3717
Size: 1533 9478
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3416 8821 278
Size: 53 83 186 936 1880 5405 2468
Fragments arranged by size:
5405 2468 1880 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4074 8354 1937
Size: 2137 4280 4594
Fragments arranged by size:
4594 4280 2137
Acc65I G'GTAC_C
Cuts at: 2750 8332 2750
Size: 5582 5429
AclI AA'CG_TT
Cuts at: 1563 9138 9511 1563
Size: 7575 373 3063
Fragments arranged by size:
7575 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 2380 2777 3094 3755 5045 6145 6786
Size: 626 122 397 317 661 1290 1100 641
Cuts at: 6786 7323 9074 10458 10726 1632
Size: 537 1751 1384 268 1917
Fragments arranged by size:
1917 1751 1384 1290 1100 661 641 626
537 397 317 268 122
AfeI AGC'GCT
Cuts at: 5071 5981 5071
Size: 910 10101
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10814
AflIII A'CryG_T
Cuts at: 1203 5139 7233 9910 10887 1203
Size: 3936 2094 2677 977 1327
Fragments arranged by size:
3936 2677 2094 1327 977
AgeI A'CCGG_T
Cuts at: 9842 9842
Size: 11011
AhdI GACnn_n'nnGTC
Cuts at: 2739 7372 9740 2739
Size: 4633 2368 4010
Fragments arranged by size:
4633 4010 2368
AleI CACnn'nnGTG
Cuts at: 1166 7232 1166
Size: 6066 4945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6491 6696 7413 7910 1184
Size: 5307 205 717 497 4285
Fragments arranged by size:
5307 4285 717 497 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5729 5761 5729
Size: 32 10979
AlwNI CAG_nnn'CTG
Cuts at: 2403 3599 4733 4915 5053 7051 8754 10326 2403
Size: 1196 1134 182 138 1998 1703 1572 3088
Fragments arranged by size:
3088 1998 1703 1572 1196 1134 182 138
ApaI G_GGCC'C
Cuts at: 4271 6048 4271
Size: 1777 9234
ApaLI G'TGCA_C
Cuts at: 3503 9068 10224 3503
Size: 5565 1156 4290
Fragments arranged by size:
5565 4290 1156
ApoI r'AATT_y
Cuts at: 1493 3935 5792 6230 8218 8320 8546 8698 10807
Size: 2442 1857 438 1988 102 226 152 2109
Cuts at: 10807 1493
Size: 1697
Fragments arranged by size:
2442 2109 1988 1857 1697 438 226 152
102
AseI AT'TA_AT
Cuts at: 160 1032 4134 9565 160
Size: 872 3102 5431 1606
Fragments arranged by size:
5431 3102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11011
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 4631 6742 7716 8336 1870
Size: 73 79 2609 2111 974 620 4545
Fragments arranged by size:
4545 2609 2111 974 620 79 73
BamHI G'GATC_C
Cuts at: 2440 3273 8341 8708 2440
Size: 833 5068 367 4743
Fragments arranged by size:
5068 4743 833 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2372 2750 3729 5218 5486
Size: 325 217 627 585 378 979 1489 268
Cuts at: 5486 6841 7473 8332 9787 618
Size: 1355 632 859 1455 1842
Fragments arranged by size:
1842 1489 1455 1355 979 859 632 627
585 378 325 268 217
BanII G_rGCy'C
Cuts at: 1391 3246 3293 3912 4271 4439 4596 6048 6440
Size: 1855 47 619 359 168 157 1452 392
Cuts at: 6440 6822 8330 1391
Size: 382 1508 4072
Fragments arranged by size:
4072 1855 1508 1452 619 392 382 359
168 157 47
BbeI G_GCGC'C
Cuts at: 6845 6845
Size: 11011
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 5033 6216 7085 928
Size: 971 416 2718 1183 869 4854
Fragments arranged by size:
4854 2718 1183 971 869 416
BbvCI CC'TCA_GC
Cuts at: 5562 8252 5562
Size: 2690 8321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2864 2898 3347 3381 6791 6825 9202
Size: 34 927 34 449 34 3410 34 2377
Cuts at: 9202 9236 1903
Size: 34 3678
Fragments arranged by size:
3678 3410 2377 927 449 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8903 10113 8903
Size: 1210 9801
BclI T'GATC_A
Cuts at: 1548 8010 1548
Size: 6462 4549
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4280 9622
Size: 107 122 71 631 605 337 2271 5342
Cuts at: 9622 136
Size: 1525
Fragments arranged by size:
5342 2271 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5958 6135 6590 7527 11006 5958
Size: 177 455 937 3479 5963
Fragments arranged by size:
5963 3479 937 455 177
BlpI GC'TnA_GC
Cuts at: 3287 4365 4581 5378 6821 8428 3287
Size: 1078 216 797 1443 1607 5870
Fragments arranged by size:
5870 1607 1443 1078 797 216
Bme1580I G_kGCm'C
Cuts at: 1790 2377 3507 4271 5221 6048 9072 10228 1790
Size: 587 1130 764 950 827 3024 1156 2573
Fragments arranged by size:
3024 2573 1156 1130 950 827 764 587
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11011
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2817 3078 3545
Size: 416 53 83 399 24 1400 261 467
Cuts at: 3545 3752 5281 8742 9700 442
Size: 207 1529 3461 958 1753
Fragments arranged by size:
3461 1753 1529 1400 958 467 416 399
261 207 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11011
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2393 5980 6672 2393
Size: 3587 692 6732
Fragments arranged by size:
6732 3587 692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2853 2905 3230 3236 4250 4523 4721
Size: 820 794 52 325 6 1014 273 198
Cuts at: 4721 4916 5029 5183 5950 6607 6667 6798 6862
Size: 195 113 154 767 657 60 131 64
Cuts at: 6862 9671 1239
Size: 2809 2579
Fragments arranged by size:
2809 2579 1014 820 794 767 657 325
273 198 195 154 131 113 64 60
52 6
Bpu10I CC'TnA_GC
Cuts at: 4438 5562 8252 4438
Size: 1124 2690 7197
Fragments arranged by size:
7197 2690 1124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 6171 9136 10001 10299 3904
Size: 2267 2965 865 298 4616
Fragments arranged by size:
4616 2965 2267 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2134 9674 882
Size: 617 635 7540 2219
Fragments arranged by size:
7540 2219 635 617
BsaAI yAC'GTr
Cuts at: 493 1204 5142 6031 8802 493
Size: 711 3938 889 2771 2702
Fragments arranged by size:
3938 2771 2702 889 711
BsaBI GATnn'nnATC
Cuts at: 2570 4734 11005 2570
Size: 2164 6271 2576
Fragments arranged by size:
6271 2576 2164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3413 5112 6804 6842
Size: 53 83 186 936 1880 1699 1692 38
Cuts at: 6842 6948 8818 9200 9835 275
Size: 106 1870 382 635 1451
Fragments arranged by size:
1880 1870 1699 1692 1451 936 635 382
186 106 83 53 38
BsaWI w'CCGG_w
Cuts at: 3270 4001 5121 5661 6943 9444 9842 10116 10263
Size: 731 1120 540 1282 2501 398 274 147
Cuts at: 10263 10674 3270
Size: 411 3607
Fragments arranged by size:
3607 2501 1282 1120 731 540 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2693 2723 5724 5754 6422
Size: 30 165 30 1290 30 3001 30 668
Cuts at: 6422 6452 7697 7727 1178
Size: 30 1245 30 4462
Fragments arranged by size:
4462 3001 1290 1245 668 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 2634 3041 4673 4901 4904 7169 1186
Size: 1420 28 407 1632 228 3 2265 5028
Fragments arranged by size:
5028 2265 1632 1420 407 228 28 3
BseYI C'CCAG_C
Cuts at: 2720 3982 4222 4511 5289 5649 5703 6441 6837
Size: 1262 240 289 778 360 54 738 396
Cuts at: 6837 7044 7180 7334 7726 7971 10214 10710 2720
Size: 207 136 154 392 245 2243 496 3021
Fragments arranged by size:
3021 2243 1262 778 738 496 396 392
360 289 245 240 207 154 136 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 3387 4238 4314 5163 5933 6431
Size: 573 420 1231 851 76 849 770 498
Cuts at: 6431 6692 6998 7334 7519 7693 1163
Size: 261 306 336 185 174 4481
Fragments arranged by size:
4481 1231 851 849 770 573 498 420
336 306 261 185 174 76
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8106 8461 9222 10250 1540
Size: 275 186 6105 355 761 1028 2301
Fragments arranged by size:
6105 2301 1028 761 355 275 186
BsiHKAI G_wGCw'C
Cuts at: 3246 3507 4176 4335 4596 6822 9072 9157 10228
Size: 261 669 159 261 2226 2250 85 1071
Cuts at: 10228 3246
Size: 4029
Fragments arranged by size:
4029 2250 2226 1071 669 261 261 159
85
BsiWI C'GTAC_G
Cuts at: 5142 5142
Size: 11011
BsmI GAATG_Cn'
Cuts at: 3322 3634 8522 8615 3322
Size: 312 4888 93 5718
Fragments arranged by size:
5718 4888 312 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5681 6515 6731 7554 8113 5681
Size: 834 216 823 559 8579
Fragments arranged by size:
8579 834 823 559 216
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2377 3246 3293 3507 3912 4176 4271
Size: 399 587 869 47 214 405 264 95
Cuts at: 4271 4335 4439 4596 5221 6048 6440 6822 8330
Size: 64 104 157 625 827 392 382 1508
Cuts at: 8330 9072 9157 10228 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1508 1071 869 827 742 625 587
405 399 392 382 264 214 157 104
95 85 64 47
BspEI T'CCGG_A
Cuts at: 5661 6943 10674 5661
Size: 1282 3731 5998
Fragments arranged by size:
5998 3731 1282
BspHI T'CATG_A
Cuts at: 4194 6222 8226 8900 10630 4194
Size: 2028 2004 674 1730 4575
Fragments arranged by size:
4575 2028 2004 1730 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2470 3717 4623 5250 7338 8366 844
Size: 1626 1247 906 627 2088 1028 3489
Fragments arranged by size:
3489 2088 1626 1247 1028 906 627
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 2709 4900 5013 7479 8898 1394
Size: 429 301 585 2191 113 2466 1419 3507
Fragments arranged by size:
3507 2466 2191 1419 585 429 301 113
BsrDI GCAATG_nn'
Cuts at: 65 1248 3610 3870 4114 5604 6124 9506 9680
Size: 1183 2362 260 244 1490 520 3382 174
Cuts at: 9680 65
Size: 1396
Fragments arranged by size:
3382 2362 1490 1396 1183 520 260 244
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3795 4695 6024 7569 9655 9842 1700
Size: 110 1985 900 1329 1545 2086 187 2869
Fragments arranged by size:
2869 2086 1985 1545 1329 900 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11011
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11011
BssSI C'ACGA_G
Cuts at: 3229 9071 10083 3229
Size: 5842 1012 4157
Fragments arranged by size:
5842 4157 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2240 2485 7579 794
Size: 1446 245 5094 4226
Fragments arranged by size:
5094 4226 1446 245
BstBI TT'CG_AA
Cuts at: 2683 9815 2683
Size: 7132 3879
BstEII G'GTnAC_C
Cuts at: 1184 4570 6784 1184
Size: 3386 2214 5411
Fragments arranged by size:
5411 3386 2214
BstXI CCAn_nnnn'nTGG
Cuts at: 3070 4458 5422 7357 3070
Size: 1388 964 1935 6724
Fragments arranged by size:
6724 1935 1388 964
BstYI r'GATC_y
Cuts at: 1972 2202 2440 2837 3198 3273 3921 4729 5530
Size: 230 238 397 361 75 648 808 801
Cuts at: 5530 5958 6135 6590 7527 8341 8708 9093 9110
Size: 428 177 455 937 814 367 385 17
Cuts at: 9110 10562 10648 10660 11006 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 937 814 808 801 648 455
428 397 385 367 361 346 238 230
177 86 75 17 12
BstZ17I GTA'TAC
Cuts at: 4075 4075
Size: 11011
Bsu36I CC'TnA_GG
Cuts at: 6986 7344 6986
Size: 358 10653
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 4102 5307 6846 513
Size: 552 939 193 1905 1205 1539 4678
Fragments arranged by size:
4678 1905 1539 1205 939 552 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3691 4135 7450 487
Size: 683 56 2465 444 3315 4048
Fragments arranged by size:
4048 3315 2465 683 444 56
BtsI GCAGTG_nn'
Cuts at: 2241 2779 4134 4297 4523 4811 6144 6626 7668
Size: 538 1355 163 226 288 1333 482 1042
Cuts at: 7668 8527 9321 9341 2241
Size: 859 794 20 3911
Fragments arranged by size:
3911 1355 1333 1042 859 794 538 482
288 226 163 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11011
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6133 6168 529
Size: 35 5569 35 5372
Fragments arranged by size:
5569 5372 35 35
DraI TTT'AAA
Cuts at: 8315 8315
Size: 11011
DraIII CAC_nnn'GTG
Cuts at: 3511 4180 5186 3511
Size: 669 1006 9336
Fragments arranged by size:
9336 1006 669
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3536 6326 8105 10018 809
Size: 507 2220 2790 1779 1913 1802
Fragments arranged by size:
2790 2220 1913 1802 1779 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3780 4143 4155
Size: 54 1425 50 275 18 1950 363 12
Cuts at: 4155 4448 5304 5310 6036 6492 6579 7416 7519
Size: 293 856 6 726 456 87 837 103
Cuts at: 7519 7665 7833 8155 8458 9347 10976 8
Size: 146 168 322 303 889 1629 43
Fragments arranged by size:
1950 1629 1425 889 856 837 726 456
363 322 303 293 275 168 146 103
87 54 50 43 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 8458 1537
Size: 275 6646 4090
Fragments arranged by size:
6646 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2601 2661 2753 3665 4043 4391 6962 7095
Size: 1248 60 92 912 378 348 2571 133
Cuts at: 7095 7610 8941 1353
Size: 515 1331 3423
Fragments arranged by size:
3423 2571 1331 1248 912 515 378 348
133 92 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2046 2052 2058 4874 9598 9972
Size: 1064 138 457 6 6 2816 4724 374
Cuts at: 9972 10118 387
Size: 146 1280
Fragments arranged by size:
4724 2816 1280 1064 457 374 146 138
6 6
EcoICRI GAG'CTC
Cuts at: 3244 4594 6820 3244
Size: 1350 2226 7435
Fragments arranged by size:
7435 2226 1350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2258 2380 2777 2853 2905 3094
Size: 393 427 199 122 397 76 52 189
Cuts at: 3094 3230 3236 3755 4250 4523 4721 4916 5029
Size: 136 6 519 495 273 198 195 113
Cuts at: 5029 5045 5183 5950 6145 6607 6667 6786 6798
Size: 16 138 767 195 462 60 119 12
Cuts at: 6798 6862 7323 9074 9671 10458 10726 1239
Size: 64 461 1751 597 787 268 1524
Fragments arranged by size:
1751 1524 787 767 597 519 495 462
461 427 397 393 273 268 199 198
195 195 189 138 136 122 119 113
76 64 60 52 16 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 5201 6186 5201
Size: 985 10026
EcoO109I rG'GnC_Cy
Cuts at: 3172 4267 4268 5654 5766 6045 6982 7380 3172
Size: 1095 1 1386 112 279 937 398 6803
Fragments arranged by size:
6803 1386 1095 937 398 279 112 1
EcoRI G'AATT_C
Cuts at: 8320 8698 8320
Size: 378 10633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2555 4513 4678 7633 2555
Size: 1958 165 2955 5933
Fragments arranged by size:
5933 2955 1958 165
FspI TGC'GCA
Cuts at: 5441 9517 5441
Size: 4076 6935
HaeII r_GCGC'y
Cuts at: 1678 2082 5073 5389 5983 6845 10158 1678
Size: 404 2991 316 594 862 3313 2531
Fragments arranged by size:
3313 2991 2531 862 594 404 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2693 2725 2757 3442 3474
Size: 32 135 32 1121 32 32 685 32
Cuts at: 3474 6493 6525 7358 7390 7697 7729 9652 9684
Size: 3019 32 833 32 307 32 1923 32
Cuts at: 9684 9726 9758 1373
Size: 42 32 2626
Fragments arranged by size:
3019 2626 1923 1121 833 685 307 135
42 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 5855 8355 8601 669
Size: 988 281 1707 2210 2500 246 3079
Fragments arranged by size:
3079 2500 2210 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2168 748
Size: 1420 9591
HpaI GTT'AAC
Cuts at: 8601 8601
Size: 11011
KasI G'GCGC_C
Cuts at: 6841 6841
Size: 11011
KpnI G_GTAC'C
Cuts at: 2754 8336 2754
Size: 5582 5429
MfeI C'AATT_G
Cuts at: 8610 8610
Size: 11011
MluI A'CGCG_T
Cuts at: 10887 10887
Size: 11011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3816 4185 5439 6717 8109 10125 10309 1305
Size: 2511 369 1254 1278 1392 2016 184 2007
Fragments arranged by size:
2511 2016 2007 1392 1278 1254 369 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3782 4145 4450 5306 5312
Size: 54 1425 343 1950 363 305 856 6
Cuts at: 5312 6494 6581 7418 7667 10
Size: 1182 87 837 249 3354
Fragments arranged by size:
3354 1950 1425 1182 856 837 363 343
305 249 87 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 3875 5780 6090 7232 8057 8123 8969
Size: 648 2709 1905 310 1142 825 66 846
Cuts at: 8969 9328 9487 518
Size: 359 159 2042
Fragments arranged by size:
2709 2042 1905 1142 846 825 648 359
310 159 66
MspA1I CmG'CkG
Cuts at: 1606 3982 4222 4580 5377 5917 6165 6384 6445
Size: 2376 240 358 797 540 248 219 61
Cuts at: 6445 6532 6676 7201 7624 7726 8427 9104 10252
Size: 87 144 525 423 102 701 677 1148
Cuts at: 10252 10497 1606
Size: 245 2120
Fragments arranged by size:
2376 2120 1148 797 701 677 540 525
423 358 248 245 240 219 144 102
87 61
NaeI GCC'GGC
Cuts at: 7571 7571
Size: 11011
NarI GG'CG_CC
Cuts at: 6842 6842
Size: 11011
NcoI C'CATG_G
Cuts at: 513 1065 2004 2197 5307 513
Size: 552 939 193 3110 6217
Fragments arranged by size:
6217 3110 939 552 193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11011
NgoMIV G'CCGG_C
Cuts at: 7569 7569
Size: 11011
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11011
NotI GC'GGCC_GC
Cuts at: 8458 8458
Size: 11011
NruI TCG'CGA
Cuts at: 2578 6129 2578
Size: 3551 7460
NspI r_CATG'y
Cuts at: 1132 1229 2182 2492 2815 6089 6391 6418 6886
Size: 97 953 310 323 3274 302 27 468
Cuts at: 6886 7582 7753 8369 9914 1132
Size: 696 171 616 1545 2229
Fragments arranged by size:
3274 2229 1545 953 696 616 468 323
310 302 171 97 27
PasI CC'CwG_GG
Cuts at: 1348 6067 7676 1348
Size: 4719 1609 4683
Fragments arranged by size:
4719 4683 1609
PciI A'CATG_T
Cuts at: 9910 9910
Size: 11011
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2515 5214 7051 1108
Size: 1407 2699 1837 5068
Fragments arranged by size:
5068 2699 1837 1407
PfoI T'CCnGG_A
Cuts at: 2661 5199 5526 7137 2661
Size: 2538 327 1611 6535
Fragments arranged by size:
6535 2538 1611 327
PmeI GTTT'AAAC
Cuts at: 8315 8315
Size: 11011
PmlI CAC'GTG
Cuts at: 6031 6031
Size: 11011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9080 9112 10593 10625 9080
Size: 32 1481 32 9466
Fragments arranged by size:
9466 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3172 5654 7380 3172
Size: 2482 1726 6803
Fragments arranged by size:
6803 2482 1726
PshAI GACnn'nnGTC
Cuts at: 1613 5553 1613
Size: 3940 7071
PsiI TTA'TAA
Cuts at: 8581 8581
Size: 11011
PspOMI G'GGCC_C
Cuts at: 4267 6044 4267
Size: 1777 9234
PstI C_TGCA'G
Cuts at: 830 3405 3465 3728 3921 5049 5247 5942 5979
Size: 2575 60 263 193 1128 198 695 37
Cuts at: 5979 7593 7866 8363 830
Size: 1614 273 497 3478
Fragments arranged by size:
3478 2575 1614 1128 695 497 273 263
198 193 60 37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11011
PvuII CAG'CTG
Cuts at: 3982 4222 5377 6165 6445 6676 7624 7726 3982
Size: 240 1155 788 280 231 948 102 7267
Fragments arranged by size:
7267 1155 948 788 280 240 231 102
RsrII CG'GwC_CG
Cuts at: 4530 4530
Size: 11011
SacI G_AGCT'C
Cuts at: 3246 4596 6822 3246
Size: 1350 2226 7435
Fragments arranged by size:
7435 2226 1350
SalI G'TCGA_C
Cuts at: 1936 8353 1936
Size: 6417 4594
SanDI GG'GwC_CC
Cuts at: 5654 7380 5654
Size: 1726 9285
SapI GCTCTTCn'nnn_
Cuts at: 2661 3665 4391 2661
Size: 1004 726 9281
Fragments arranged by size:
9281 1004 726
SbfI CC_TGCA'GG
Cuts at: 5247 8363 5247
Size: 3116 7895
ScaI AGT'ACT
Cuts at: 2900 4311 5936 8288 9259 10971 2900
Size: 1411 1625 2352 971 1712 2940
Fragments arranged by size:
2940 2352 1712 1625 1411 971
SexAI A'CCwGG_T
Cuts at: 3006 4176 3006
Size: 1170 9841
SfcI C'TryA_G
Cuts at: 826 1045 2434 3401 3461 3724 3917 3926 4108
Size: 219 1389 967 60 263 193 9 182
Cuts at: 4108 5045 5243 5938 5975 7589 7840 7862 8359
Size: 937 198 695 37 1614 251 22 497
Cuts at: 8359 9494 10175 10366 826
Size: 1135 681 191 1471
Fragments arranged by size:
1614 1471 1389 1135 967 937 695 681
497 263 251 219 198 193 191 182
60 37 22 9
SfoI GGC'GCC
Cuts at: 6843 6843
Size: 11011
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11011
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11011
SmaI CCC'GGG
Cuts at: 1872 2024 6744 7718 8338 1872
Size: 152 4720 974 620 4545
Fragments arranged by size:
4720 4545 974 620 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 6186 9115 10016 10278 820
Size: 197 926 1940 2303 2929 901 262 1553
Fragments arranged by size:
2929 2303 1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 8802 493
Size: 8309 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11011
SphI G_CATG'C
Cuts at: 1132 2815 6089 8369 1132
Size: 1683 3274 2280 3774
Fragments arranged by size:
3774 3274 2280 1683
SspI AAT'ATT
Cuts at: 5 52 8935 5
Size: 47 8883 2081
Fragments arranged by size:
8883 2081 47
StuI AGG'CCT
Cuts at: 1517 2513 6864 1517
Size: 996 4351 5664
Fragments arranged by size:
5664 4351 996
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 2197 2514 3861 4875 5307
Size: 552 939 147 46 317 1347 1014 432
Cuts at: 5307 6009 6352 8450 513
Size: 702 343 2098 3074
Fragments arranged by size:
3074 2098 1347 1014 939 702 552 432
343 317 147 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4366 6365 6871 7668 9038 9055 9208
Size: 1255 2786 1999 506 797 1370 17 153
Cuts at: 9208 9393 9838 325
Size: 185 445 1498
Fragments arranged by size:
2786 1999 1498 1370 1255 797 506 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2898 3159 4309
Size: 274 80 33 51 1443 921 261 1150
Cuts at: 4309 4704 5934 6597 8286 9257 10969 96
Size: 395 1230 663 1689 971 1712 138
Fragments arranged by size:
1712 1689 1443 1230 1150 971 921 663
395 274 261 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3735 3767 4413 4445 5724 5756 10971 11003 3735
Size: 32 646 32 1279 32 5215 32 3743
Fragments arranged by size:
5215 3743 1279 646 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3517 4568 5053 8102 1316
Size: 2201 1051 485 3049 4225
Fragments arranged by size:
4225 3049 2201 1051 485
XbaI T'CTAG_A
Cuts at: 7093 8347 7093
Size: 1254 9757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2334 3596 7552 7928 2334
Size: 1262 3956 376 5417
Fragments arranged by size:
5417 3956 1262 376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11011
XmaI C'CCGG_G
Cuts at: 1870 2022 6742 7716 8336 1870
Size: 152 4720 974 620 4545
Fragments arranged by size:
4720 4545 974 620 152
XmnI GAAnn'nnTTC
Cuts at: 2679 2689 2988 4037 4385 5171 7616 9140 2679
Size: 10 299 1049 348 786 2445 1524 4550
Fragments arranged by size:
4550 2445 1524 1049 786 348 299 10
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3414 8819 276
Size: 53 83 186 936 1880 5405 2468
Fragments arranged by size:
5405 2468 1880 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI EcoRV FseI FspAI NsiI
PacI PspXI PsrI SacII SfiI SrfI SwaI