Restriction map for
pFN21AB1389
(Circular) (Six-base) MAPSORT of: pFN21AB1389.seq Check: 238 from: 1 to: 6649
pFN21AB1389 6649 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4459 278
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3992 1937
Size: 2055 4594
Acc65I G'GTAC_C
Cuts at: 3970 3970
Size: 6649
AclI AA'CG_TT
Cuts at: 1563 4776 5149 1563
Size: 3213 373 3063
Fragments arranged by size:
3213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3034 3226 3610 4712 6096 6364 1632
Size: 1402 192 384 1102 1384 268 1917
Fragments arranged by size:
1917 1402 1384 1102 384 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6452
AflIII A'CryG_T
Cuts at: 1203 2700 5548 6525 1203
Size: 1497 2848 977 1327
Fragments arranged by size:
2848 1497 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 5378 5378
Size: 6649
AleI CACnn'nnGTG
Cuts at: 1166 3098 1166
Size: 1932 4717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6649
AlwNI CAG_nnn'CTG
Cuts at: 2170 2557 4392 5964 2170
Size: 387 1835 1572 2855
Fragments arranged by size:
2855 1835 1572 387
ApaI G_GGCC'C
Cuts at: 2221 2253 2946 2221
Size: 32 693 5924
Fragments arranged by size:
5924 693 32
ApaLI G'TGCA_C
Cuts at: 2377 2456 2536 2666 4706 5862 2377
Size: 79 80 130 2040 1156 3164
Fragments arranged by size:
3164 2040 1156 130 80 79
ApoI r'AATT_y
Cuts at: 1493 3958 4184 4336 6445 1493
Size: 2465 226 152 2109 1697
Fragments arranged by size:
2465 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2159 2159
Size: 6649
AseI AT'TA_AT
Cuts at: 160 1032 5203 160
Size: 872 4171 1606
Fragments arranged by size:
4171 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2252 2794 2945 3974 1870
Size: 73 91 218 542 151 1029 4545
Fragments arranged by size:
4545 1029 542 218 151 91 73
BamHI G'GATC_C
Cuts at: 3979 4346 3979
Size: 367 6282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2672 2714 2778 2801
Size: 325 217 627 233 652 42 64 23
Cuts at: 2801 2851 3194 3484 3578 3773 3970 5425 618
Size: 50 343 290 94 195 197 1455 1842
Fragments arranged by size:
1842 1455 652 627 343 325 290 233
217 197 195 94 64 50 42 23
BanII G_rGCy'C
Cuts at: 1391 2221 2253 2502 2931 2946 3800 3968 1391
Size: 830 32 249 429 15 854 168 4072
Fragments arranged by size:
4072 854 830 429 249 168 32 15
BbeI G_GCGC'C
Cuts at: 2024 2676 2805 3488 3777 2024
Size: 652 129 683 289 4896
Fragments arranged by size:
4896 683 652 289 129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3141 3616 3805 928
Size: 971 1242 475 189 3772
Fragments arranged by size:
3772 1242 971 475 189
BbvCI CC'TCA_GC
Cuts at: 2010 2286 2760 2010
Size: 276 474 5899
Fragments arranged by size:
5899 474 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2368 2402 4840 4874 1903
Size: 34 431 34 2438 34 3678
Fragments arranged by size:
3678 2438 431 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4541 5751 4541
Size: 1210 5439
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 2225 3362
Size: 107 122 71 631 605 421 132 1137
Cuts at: 3362 5260 136
Size: 1898 1525
Fragments arranged by size:
1898 1525 1137 631 605 421 132 122
107 71
BglII A'GATC_T
Cuts at: 6644 6644
Size: 6649
BlpI GC'TnA_GC
Cuts at: 2496 3425 4066 2496
Size: 929 641 5079
Fragments arranged by size:
5079 929 641
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2253 2381 2460 2540 2670 2717 2781
Size: 431 32 128 79 80 130 47 64
Cuts at: 2781 2856 2946 3178 3197 4710 5866 1790
Size: 75 90 232 19 1513 1156 2573
Fragments arranged by size:
2573 1513 1156 431 232 130 128 90
80 79 75 64 47 32 19
BmgBI CAC'GTC
Cuts at: 1318 2703 3516 3855 1318
Size: 1385 813 339 4112
Fragments arranged by size:
4112 1385 813 339
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4380 5338 442
Size: 416 53 83 399 24 2963 958 1753
Fragments arranged by size:
2963 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2471 3617 3743 3916 5309 1239
Size: 971 261 1146 126 173 1393 2579
Fragments arranged by size:
2579 1393 1146 971 261 173 126
Bpu10I CC'TnA_GC
Cuts at: 2010 2286 2760 2010
Size: 276 474 5899
Fragments arranged by size:
5899 474 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 2630 2831 4774 5639 5937 2486
Size: 144 201 1943 865 298 3198
Fragments arranged by size:
3198 1943 865 298 201 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5312 882
Size: 617 3813 2219
Fragments arranged by size:
3813 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3027 4440 493
Size: 711 1823 1413 2702
Fragments arranged by size:
2702 1823 1413 711
BsaBI GATnn'nnATC
Cuts at: 6643 6643
Size: 6649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2115 2673 2723
Size: 53 83 186 936 488 94 558 50
Cuts at: 2723 2802 3485 3621 3774 4456 4838 5473 275
Size: 79 683 136 153 682 382 635 1451
Fragments arranged by size:
1451 936 683 682 635 558 488 382
186 153 136 94 83 79 53 50
BsaWI w'CCGG_w
Cuts at: 5082 5480 5754 5901 6312 5082
Size: 398 274 147 411 5419
Fragments arranged by size:
5419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6424
Fragments arranged by size:
6424 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2030 2114 2963 1186
Size: 841 3 84 849 4872
Fragments arranged by size:
4872 849 841 84 3
BseYI C'CCAG_C
Cuts at: 2058 2206 2419 3400 3711 5852 6348 2058
Size: 148 213 981 311 2141 496 2359
Fragments arranged by size:
2359 2141 981 496 311 213 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 3196 1163
Size: 573 768 692 4616
Fragments arranged by size:
4616 768 692 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3099 4099 4860 5888 1540
Size: 275 186 1098 1000 761 1028 2301
Fragments arranged by size:
2301 1098 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2381 2460 2502 2519 2540 2670 4710 4795 5866
Size: 79 42 17 21 130 2040 85 1071
Cuts at: 5866 2381
Size: 3164
Fragments arranged by size:
3164 2040 1071 130 85 79 42 21
17
BsmI GAATG_Cn'
Cuts at: 4160 4253 4160
Size: 93 6556
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2253 2381 2460 2502 2519 2540
Size: 399 431 32 128 79 42 17 21
Cuts at: 2540 2670 2717 2781 2856 2931 2946 3178 3197
Size: 130 47 64 75 75 15 232 19
Cuts at: 3197 3800 3968 4710 4795 5866 1391
Size: 603 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 431 399 232 168
130 128 85 79 75 75 64 47
42 32 21 19 17 15
BspEI T'CCGG_A
Cuts at: 6312 6312
Size: 6649
BspHI T'CATG_A
Cuts at: 2610 3225 3564 3816 4538 6268 2610
Size: 615 339 252 722 1730 2991
Fragments arranged by size:
2991 1730 722 615 339 252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 4004 844
Size: 1580 1580 3489
Fragments arranged by size:
3489 1580 1580
BsrBI CCG'CTC
Cuts at: 1394 1823 2301 2323 2571 3064 3429 4536 1394
Size: 429 478 22 248 493 365 1107 3507
Fragments arranged by size:
3507 1107 493 478 429 365 248 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 5144 5318 65
Size: 1183 3896 174 1396
Fragments arranged by size:
3896 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2117 2675 2804 3119 3370 3530
Size: 110 232 75 558 129 315 251 160
Cuts at: 3530 5293 5480 1700
Size: 1763 187 2869
Fragments arranged by size:
2869 1763 558 315 251 232 187 160
129 110 75
BsrGI T'GTAC_A
Cuts at: 96 3636 96
Size: 3540 3109
BssHII G'CGCG_C
Cuts at: 1925 2159 2584 2586 2588 2839 1925
Size: 234 425 2 2 251 5735
Fragments arranged by size:
5735 425 251 234 2 2
BssSI C'ACGA_G
Cuts at: 3317 3910 4709 5721 3317
Size: 593 799 1012 4245
Fragments arranged by size:
4245 1012 799 593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2557 794
Size: 1763 4886
BstBI TT'CG_AA
Cuts at: 5453 5453
Size: 6649
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6649
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2745 2419
Size: 326 6323
BstYI r'GATC_y
Cuts at: 1972 3867 3979 4346 4731 4748 6200 6286 6298
Size: 1895 112 367 385 17 1452 86 12
Cuts at: 6298 6644 1972
Size: 346 1977
Fragments arranged by size:
1977 1895 1452 385 367 346 112 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2344 2782 3099 3105 513
Size: 552 939 340 438 317 6 4057
Fragments arranged by size:
4057 939 552 438 340 317 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2371 3100 3432 3646 487
Size: 683 56 1145 729 332 214 3490
Fragments arranged by size:
3490 1145 729 683 332 214 56
BtsI GCAGTG_nn'
Cuts at: 2492 4165 4959 4979 2492
Size: 1673 794 20 4162
Fragments arranged by size:
4162 1673 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2333 2368 529
Size: 35 1769 35 4810
Fragments arranged by size:
4810 1769 35 35
DraI TTT'AAA
Cuts at: 3953 3953
Size: 6649
DraIII CAC_nnn'GTG
Cuts at: 2906 2906
Size: 6649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3809 5656 809
Size: 507 2493 1847 1802
Fragments arranged by size:
2493 1847 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2633 3096
Size: 54 1425 50 275 18 292 511 463
Cuts at: 3096 3102 3249 3354 3372 3528 4096 4985 6614
Size: 6 147 105 18 156 568 889 1629
Cuts at: 6614 8
Size: 43
Fragments arranged by size:
1629 1425 889 568 511 463 292 275
156 147 105 54 50 43 18 18
6
EagI C'GGCC_G
Cuts at: 1537 1812 3096 4096 1537
Size: 275 1284 1000 4090
Fragments arranged by size:
4090 1284 1000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3017 3227 3497 3551 4579 1353
Size: 1664 210 270 54 1028 3423
Fragments arranged by size:
3423 1664 1028 270 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 2787 3783 5236 5610 5756
Size: 1064 138 522 676 996 1453 374 146
Cuts at: 5756 387
Size: 1280
Fragments arranged by size:
1453 1280 1064 996 676 522 374 146
138
EcoICRI GAG'CTC
Cuts at: 2500 2500
Size: 6649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2471 3034 3226 3610 3617 3743
Size: 393 578 261 563 192 384 7 126
Cuts at: 3743 3916 4712 5309 6096 6364 1239
Size: 173 796 597 787 268 1524
Fragments arranged by size:
1524 796 787 597 578 563 393 384
268 261 192 173 126 7
EcoO109I rG'GnC_Cy
Cuts at: 2249 2249
Size: 6649
EcoRI G'AATT_C
Cuts at: 3958 4336 3958
Size: 378 6271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 6649
FspI TGC'GCA
Cuts at: 3614 5155 3614
Size: 1541 5108
FspAI rTGC'GCAy
Cuts at: 3614 3614
Size: 6649
HaeII r_GCGC'y
Cuts at: 1678 2024 2081 2558 2609 2676 2805 3049 3401
Size: 346 57 477 51 67 129 244 352
Cuts at: 3401 3488 3777 5796 1678
Size: 87 289 2019 2531
Fragments arranged by size:
2531 2019 477 352 346 289 244 129
87 67 57 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5290 5322 5364 5396 1373
Size: 32 135 32 3718 32 42 32 2626
Fragments arranged by size:
3718 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 3993 4239 669
Size: 988 281 205 1850 246 3079
Fragments arranged by size:
3079 1850 988 281 246 205
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6649
HpaI GTT'AAC
Cuts at: 4239 4239
Size: 6649
KasI G'GCGC_C
Cuts at: 2020 2672 2801 3484 3773 2020
Size: 652 129 683 289 4896
Fragments arranged by size:
4896 683 652 289 129
KpnI G_GTAC'C
Cuts at: 3974 3974
Size: 6649
MfeI C'AATT_G
Cuts at: 4248 4248
Size: 6649
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 2793 5763 5947 1305
Size: 1425 63 2970 184 2007
Fragments arranged by size:
2970 2007 1425 184 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2635 3251 10
Size: 54 1425 343 803 616 3408
Fragments arranged by size:
3408 1425 803 616 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 3563 4607 4966 5125 518
Size: 648 1932 465 1044 359 159 2042
Fragments arranged by size:
2042 1932 1044 648 465 359 159
MspA1I CmG'CkG
Cuts at: 1606 2109 2167 2200 2374 2495 3711 4065 4742
Size: 503 58 33 174 121 1216 354 677
Cuts at: 4742 5890 6135 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1216 1148 677 503 354 245 174
121 58 33
NaeI GCC'GGC
Cuts at: 2044 2677 2806 3121 3372 3532 2044
Size: 633 129 315 251 160 5161
Fragments arranged by size:
5161 633 315 251 160 129
NarI GG'CG_CC
Cuts at: 2021 2673 2802 3485 3774 2021
Size: 652 129 683 289 4896
Fragments arranged by size:
4896 683 652 289 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5158
Fragments arranged by size:
5158 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6649
NgoMIV G'CCGG_C
Cuts at: 2042 2675 2804 3119 3370 3530 2042
Size: 633 129 315 251 160 5161
Fragments arranged by size:
5161 633 315 251 160 129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 3043 4007 5552 1132
Size: 97 1814 964 1545 2229
Fragments arranged by size:
2229 1814 1545 964 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6649
PciI A'CATG_T
Cuts at: 5548 5548
Size: 6649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2878 3264 1108
Size: 1770 386 4493
Fragments arranged by size:
4493 1770 386
PfoI T'CCnGG_A
Cuts at: 2337 3129 3690 3893 2337
Size: 792 561 203 5093
Fragments arranged by size:
5093 792 561 203
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2489 2521 4718 4750 6231 6263 2489
Size: 32 2197 32 1481 32 2875
Fragments arranged by size:
2875 2197 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3944 1613
Size: 2331 4318
PsiI TTA'TAA
Cuts at: 4219 4219
Size: 6649
PspOMI G'GGCC_C
Cuts at: 2217 2249 2942 2217
Size: 32 693 5924
Fragments arranged by size:
5924 693 32
PstI C_TGCA'G
Cuts at: 830 4001 830
Size: 3171 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
PvuII CAG'CTG
Cuts at: 2167 3711 2167
Size: 1544 5105
SacI G_AGCT'C
Cuts at: 2502 2502
Size: 6649
SalI G'TCGA_C
Cuts at: 1936 3991 1936
Size: 2055 4594
SapI GCTCTTCn'nnn_
Cuts at: 3017 3551 3017
Size: 534 6115
SbfI CC_TGCA'GG
Cuts at: 4001 4001
Size: 6649
ScaI AGT'ACT
Cuts at: 4897 6609 4897
Size: 1712 4937
SexAI A'CCwGG_T
Cuts at: 3030 3284 3030
Size: 254 6395
SfcI C'TryA_G
Cuts at: 826 1045 3942 3997 5132 5813 6004 826
Size: 219 2897 55 1135 681 191 1471
Fragments arranged by size:
2897 1471 1135 681 219 191 55
SfoI GGC'GCC
Cuts at: 2022 2674 2803 3486 3775 2022
Size: 652 129 683 289 4896
Fragments arranged by size:
4896 683 652 289 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 2042 2117 2675 2804 1700
Size: 342 75 558 129 5545
Fragments arranged by size:
5545 558 342 129 75
SmaI CCC'GGG
Cuts at: 1872 2036 2254 2796 3976 1872
Size: 164 218 542 1180 4545
Fragments arranged by size:
4545 1180 542 218 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 2645 2846 4753 5654 5916
Size: 197 926 558 144 201 1907 901 262
Cuts at: 5916 820
Size: 1553
Fragments arranged by size:
1907 1553 926 901 558 262 201 197
144
SnaBI TAC'GTA
Cuts at: 493 3027 4440 493
Size: 2534 1413 2702
Fragments arranged by size:
2702 2534 1413
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6649
SphI G_CATG'C
Cuts at: 1132 4007 1132
Size: 2875 3774
SrfI GCCC'GGGC
Cuts at: 2254 2254
Size: 6649
SspI AAT'ATT
Cuts at: 5 52 4573 5
Size: 47 4521 2081
Fragments arranged by size:
4521 2081 47
StuI AGG'CCT
Cuts at: 1517 3686 1517
Size: 2169 4480
StyI C'CwwG_G
Cuts at: 513 1065 2004 2916 4088 513
Size: 552 939 912 1172 3074
Fragments arranged by size:
3074 1172 939 912 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3153 3256 3602 3703 4676 4693 4846
Size: 1255 1573 103 346 101 973 17 153
Cuts at: 4846 5031 5476 325
Size: 185 445 1498
Fragments arranged by size:
1573 1498 1255 973 445 346 185 153
103 101 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2934 3636
Size: 274 80 33 51 1443 333 624 702
Cuts at: 3636 4895 6607 96
Size: 1259 1712 138
Fragments arranged by size:
1712 1443 1259 702 624 333 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6609 6641 6609
Size: 32 6617
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6649
XbaI T'CTAG_A
Cuts at: 3985 3985
Size: 6649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3391 3397 3391
Size: 6 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 2034 2252 2794 3974 1870
Size: 164 218 542 1180 4545
Fragments arranged by size:
4545 1180 542 218 164
XmnI GAAnn'nnTTC
Cuts at: 2618 4778 2618
Size: 2160 4489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4457 276
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SfiI SwaI