Restriction map for
pFN21AB1380
(Circular) (Six-base) MAPSORT of: pFN21AB1380.seq Check: 5886 from: 1 to: 6826
pFN21AB1380 6826 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4636 278
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4169 1937
Size: 2232 4594
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 2458 2710 3130 3802 4889 6273 6541
Size: 478 348 252 420 672 1087 1384 268
Cuts at: 6541 1632
Size: 1917
Fragments arranged by size:
1917 1384 1087 672 478 420 348 268
252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6629
AflIII A'CryG_T
Cuts at: 1203 2534 2625 2963 5725 6702 1203
Size: 1331 91 338 2762 977 1327
Fragments arranged by size:
2762 1331 1327 977 338 91
AgeI A'CCGG_T
Cuts at: 5657 5657
Size: 6826
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6826
AlwNI CAG_nnn'CTG
Cuts at: 3685 4569 6141 3685
Size: 884 1572 4370
Fragments arranged by size:
4370 1572 884
ApaI G_GGCC'C
Cuts at: 3409 3409
Size: 6826
ApaLI G'TGCA_C
Cuts at: 3770 4883 6039 3770
Size: 1113 1156 4557
Fragments arranged by size:
4557 1156 1113
ApoI r'AATT_y
Cuts at: 1493 2233 2639 3238 3490 3742 3910 4135 4361
Size: 740 406 599 252 252 168 225 226
Cuts at: 4361 4513 6622 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 740 599 406 252 252 226
225 168 152
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 4151 1870
Size: 73 2208 4545
Fragments arranged by size:
4545 2208 73
BamHI G'GATC_C
Cuts at: 4156 4523 4156
Size: 367 6459
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4147 5602 618
Size: 325 217 627 2360 1455 1842
Fragments arranged by size:
2360 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3409 4145 1391
Size: 2018 736 4072
Fragments arranged by size:
4072 2018 736
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 2216 928
Size: 971 145 172 5538
Fragments arranged by size:
5538 971 172 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5017 5051 1903
Size: 34 3080 34 3678
Fragments arranged by size:
3678 3080 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2161 4718 5928 2161
Size: 2557 1210 3059
Fragments arranged by size:
3059 2557 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BfrBI ATG'CAT
Cuts at: 4012 4012
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5437 136
Size: 107 122 71 631 605 3765 1525
Fragments arranged by size:
3765 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3076 6821 3076
Size: 3745 3081
BlpI GC'TnA_GC
Cuts at: 4243 4243
Size: 6826
Bme1580I G_kGCm'C
Cuts at: 1790 3409 3774 4887 6043 1790
Size: 1619 365 1113 1156 2573
Fragments arranged by size:
2573 1619 1156 1113 365
BmgBI CAC'GTC
Cuts at: 1318 2041 2626 1318
Size: 723 585 5518
Fragments arranged by size:
5518 723 585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4557 5515 442
Size: 416 53 83 399 24 3140 958 1753
Fragments arranged by size:
3140 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3501 3501
Size: 6826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2706 3546 3798 5486 1239
Size: 899 568 840 252 1688 2579
Fragments arranged by size:
2579 1688 899 840 568 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2331 4066 4951 5816 6114 2331
Size: 1735 885 865 298 3043
Fragments arranged by size:
3043 1735 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2349 3610 5489 882
Size: 617 850 1261 1879 2219
Fragments arranged by size:
2219 1879 1261 850 617
BsaAI yAC'GTr
Cuts at: 493 1204 2535 2964 4617 493
Size: 711 1331 429 1653 2702
Fragments arranged by size:
2702 1653 1331 711 429
BsaBI GATnn'nnATC
Cuts at: 2499 4011 6820 2499
Size: 1512 2809 2505
Fragments arranged by size:
2809 2505 1512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3853 4633 5015 5650
Size: 53 83 186 936 2320 780 382 635
Cuts at: 5650 275
Size: 1451
Fragments arranged by size:
2320 1451 936 780 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5259 5657 5931 6078 6489 5259
Size: 398 274 147 411 5596
Fragments arranged by size:
5596 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6601
Fragments arranged by size:
6601 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3059 1186
Size: 1873 4953
BseYI C'CCAG_C
Cuts at: 2068 2823 3654 6029 6525 2068
Size: 755 831 2375 496 2369
Fragments arranged by size:
2375 2369 831 755 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3671 1163
Size: 573 1935 4318
Fragments arranged by size:
4318 1935 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4276 5037 6065 1540
Size: 275 186 2275 761 1028 2301
Fragments arranged by size:
2301 2275 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3774 4887 4972 6043 3774
Size: 1113 85 1071 4557
Fragments arranged by size:
4557 1113 1071 85
BsmI GAATG_Cn'
Cuts at: 2156 2801 2920 4337 4430 2156
Size: 645 119 1417 93 4552
Fragments arranged by size:
4552 1417 645 119 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3844 3844
Size: 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3409 3774 4145 4887 4972 6043 1391
Size: 399 1619 365 371 742 85 1071 2174
Fragments arranged by size:
2174 1619 1071 742 399 371 365 85
BspEI T'CCGG_A
Cuts at: 6489 6489
Size: 6826
BspHI T'CATG_A
Cuts at: 4715 6445 4715
Size: 1730 5096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 4181 844
Size: 1298 2039 3489
Fragments arranged by size:
3489 2039 1298
BsrBI CCG'CTC
Cuts at: 1394 1823 4713 1394
Size: 429 2890 3507
Fragments arranged by size:
3507 2890 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2031 2461 5321 5495 65
Size: 1183 783 430 2860 174 1396
Fragments arranged by size:
2860 1396 1183 783 430 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5470 5657 1700
Size: 110 3660 187 2869
Fragments arranged by size:
3660 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3904 96
Size: 3808 3018
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 3157 3661 4886 5898 3157
Size: 504 1225 1012 4085
Fragments arranged by size:
4085 1225 1012 504
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6826
BstBI TT'CG_AA
Cuts at: 5630 5630
Size: 6826
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6826
BstXI CCAn_nnnn'nTGG
Cuts at: 2456 2456
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 2247 3076 3263 3328 4156 4523 4908 4925
Size: 275 829 187 65 828 367 385 17
Cuts at: 4925 6377 6463 6475 6821 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 829 828 385 367 346 275
187 86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2183 2183
Size: 6826
BtgI C'CryG_G
Cuts at: 513 1065 2925 513
Size: 552 1860 4414
Fragments arranged by size:
4414 1860 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2990 487
Size: 683 56 1764 4323
Fragments arranged by size:
4323 1764 683 56
BtsI GCAGTG_nn'
Cuts at: 4342 5136 5156 4342
Size: 794 20 6012
Fragments arranged by size:
6012 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6791
DraI TTT'AAA
Cuts at: 4130 4130
Size: 6826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5833 809
Size: 507 4517 1802
Fragments arranged by size:
4517 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4273 5162 6791
Size: 54 1425 50 275 18 2443 889 1629
Cuts at: 6791 8
Size: 43
Fragments arranged by size:
2443 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4273 1537
Size: 275 2461 4090
Fragments arranged by size:
4090 2461 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 3134 3386 3459 3470 3638 3711 4756
Size: 705 1076 252 73 11 168 73 1045
Cuts at: 4756 1353
Size: 3423
Fragments arranged by size:
3423 1076 1045 705 252 168 73 73
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5413 5787 5933 387
Size: 1064 138 3824 374 146 1280
Fragments arranged by size:
3824 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2138 2458 2706 2710 3130 3546
Size: 393 478 28 320 248 4 420 416
Cuts at: 3546 3798 3802 4889 5486 6273 6541 1239
Size: 252 4 1087 597 787 268 1524
Fragments arranged by size:
1524 1087 787 597 478 420 416 393
320 268 252 248 28 4 4
EcoO109I rG'GnC_Cy
Cuts at: 3406 3406
Size: 6826
EcoRI G'AATT_C
Cuts at: 2233 2639 3742 3910 4135 4513 2233
Size: 406 1103 168 225 378 4546
Fragments arranged by size:
4546 1103 406 378 225 168
FspI TGC'GCA
Cuts at: 5332 5332
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 3997 5973 1678
Size: 2319 1976 2531
Fragments arranged by size:
2531 2319 1976
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 2641 2673 2965
Size: 32 135 32 660 32 377 32 292
Cuts at: 2965 2997 3313 3345 3385 3417 5467 5499 5541
Size: 32 316 32 40 32 2050 32 42
Cuts at: 5541 5573 1373
Size: 32 2626
Fragments arranged by size:
2626 2050 660 377 316 292 135 42
40 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4170 4416 669
Size: 988 281 2232 246 3079
Fragments arranged by size:
3079 2232 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6826
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 3625 4425 3625
Size: 800 6026
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3306 4093 5940 6124 1305
Size: 2001 787 1847 184 2007
Fragments arranged by size:
2007 2001 1847 787 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5004
Fragments arranged by size:
5004 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2535 2869 2953 3037 3205 3289 3457
Size: 648 1369 334 84 84 168 84 168
Cuts at: 3457 3541 3709 3793 3961 4045 4101 4784 5143
Size: 84 168 84 168 84 56 683 359
Cuts at: 5143 5302 518
Size: 159 2042
Fragments arranged by size:
2042 1369 683 648 359 334 168 168
168 168 159 84 84 84 84 84
84 56
MspA1I CmG'CkG
Cuts at: 1606 3822 4242 4919 6067 6312 1606
Size: 2216 420 677 1148 245 2120
Fragments arranged by size:
2216 2120 1148 677 420 245
NcoI C'CATG_G
Cuts at: 513 1065 2925 513
Size: 552 1860 4414
Fragments arranged by size:
4414 1860 552
NdeI CA'TA_TG
Cuts at: 387 3093 387
Size: 2706 4120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 4273 4273
Size: 6826
NsiI A_TGCA'T
Cuts at: 4014 4014
Size: 6826
NspI r_CATG'y
Cuts at: 1132 1229 4184 5729 1132
Size: 97 2955 1545 2229
Fragments arranged by size:
2955 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2449 1348
Size: 1101 5725
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6826
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 2681 2713 4895 4927 6408 6440 2123
Size: 32 526 32 2182 32 1481 32 2509
Fragments arranged by size:
2509 2182 1481 526 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 2784 4396 2784
Size: 1612 5214
PspOMI G'GGCC_C
Cuts at: 3405 3405
Size: 6826
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2546 2578 2546
Size: 32 6794
PstI C_TGCA'G
Cuts at: 830 4178 830
Size: 3348 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 3822 3822
Size: 6826
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SapI GCTCTTCn'nnn_
Cuts at: 2058 3134 3386 2058
Size: 1076 252 5498
Fragments arranged by size:
5498 1076 252
SbfI CC_TGCA'GG
Cuts at: 4178 4178
Size: 6826
ScaI AGT'ACT
Cuts at: 5074 6786 5074
Size: 1712 5114
SexAI A'CCwGG_T
Cuts at: 2339 2339
Size: 6826
SfcI C'TryA_G
Cuts at: 826 1045 4174 5309 5990 6181 826
Size: 219 3129 1135 681 191 1471
Fragments arranged by size:
3129 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 4153 1872
Size: 2281 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2346 4081 4930 5831 6093 820
Size: 197 926 403 1735 849 901 262 1553
Fragments arranged by size:
1735 1553 926 901 849 403 262 197
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SspI AAT'ATT
Cuts at: 5 52 4750 5
Size: 47 4698 2081
Fragments arranged by size:
4698 2081 47
StuI AGG'CCT
Cuts at: 1517 2807 4079 1517
Size: 1290 1272 4264
Fragments arranged by size:
4264 1290 1272
StyI C'CwwG_G
Cuts at: 513 1065 2925 4265 513
Size: 552 1860 1340 3074
Fragments arranged by size:
3074 1860 1340 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4853 4870 5023 5208 5653 325
Size: 1255 3273 17 153 185 445 1498
Fragments arranged by size:
3273 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3904 5072 6784
Size: 274 80 33 51 1443 1927 1168 1712
Cuts at: 6784 96
Size: 138
Fragments arranged by size:
1927 1712 1443 1168 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 6786 6818 3248
Size: 32 3506 32 3256
Fragments arranged by size:
3506 3256 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6826
XbaI T'CTAG_A
Cuts at: 4162 4162
Size: 6826
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6826
XmaI C'CCGG_G
Cuts at: 1870 4151 1870
Size: 2281 4545
XmnI GAAnn'nnTTC
Cuts at: 2696 2723 2807 2864 2948 3143 3815 3914 3936
Size: 27 84 57 84 195 672 99 22
Cuts at: 3936 4955 2696
Size: 1019 4567
Fragments arranged by size:
4567 1019 672 195 99 84 84 57
27 22
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4634 276
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI XcmI