Restriction map for
pFN21AB1326
(Circular) (Six-base) MAPSORT of: pFN21AB1326.seq Check: 8273 from: 1 to: 7531
pFN21AB1326 7531 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3436 4393 4657 3436
Size: 957 264 6310
Fragments arranged by size:
6310 957 264
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2277 5341 278
Size: 53 83 186 936 741 3064 2468
Fragments arranged by size:
3064 2468 936 741 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4874 1937
Size: 2937 4594
Acc65I G'GTAC_C
Cuts at: 3987 4852 3987
Size: 865 6666
AclI AA'CG_TT
Cuts at: 1563 5658 6031 1563
Size: 4095 373 3063
Fragments arranged by size:
4095 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2755 2924 3221 3701 5594 6978 7246
Size: 695 428 169 297 480 1893 1384 268
Cuts at: 7246 1632
Size: 1917
Fragments arranged by size:
1917 1893 1384 695 480 428 297 268
169
AfeI AGC'GCT
Cuts at: 2845 2845
Size: 7531
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7334
AflIII A'CryG_T
Cuts at: 1203 3240 6430 7407 1203
Size: 2037 3190 977 1327
Fragments arranged by size:
3190 2037 1327 977
AgeI A'CCGG_T
Cuts at: 6362 6362
Size: 7531
AhdI GACnn_n'nnGTC
Cuts at: 6260 6260
Size: 7531
AleI CACnn'nnGTG
Cuts at: 1166 2333 3590 4661 1166
Size: 1167 1257 1071 4036
Fragments arranged by size:
4036 1257 1167 1071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2102 3575 1184
Size: 918 1473 5140
Fragments arranged by size:
5140 1473 918
AlwNI CAG_nnn'CTG
Cuts at: 2505 3403 4721 5274 6846 2505
Size: 898 1318 553 1572 3190
Fragments arranged by size:
3190 1572 1318 898 553
ApaI G_GGCC'C
Cuts at: 2032 2168 3010 3190 2032
Size: 136 842 180 6373
Fragments arranged by size:
6373 842 180 136
ApaLI G'TGCA_C
Cuts at: 2326 4733 5588 6744 2326
Size: 2407 855 1156 3113
Fragments arranged by size:
3113 2407 1156 855
ApoI r'AATT_y
Cuts at: 1493 4840 5066 5218 7327 1493
Size: 3347 226 152 2109 1697
Fragments arranged by size:
3347 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6085 160
Size: 872 5053 1606
Fragments arranged by size:
5053 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7531
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2113 2138 2161 2382 2581 2699
Size: 73 81 89 25 23 221 199 118
Cuts at: 2699 3020 3134 3326 3539 3887 4474 4696 4791
Size: 321 114 192 213 348 587 222 95
Cuts at: 4791 4856 1870
Size: 65 4545
Fragments arranged by size:
4545 587 348 321 222 221 213 199
192 118 114 95 89 81 73 65
25 23
AvrII C'CTAG_G
Cuts at: 4826 4826
Size: 7531
BamHI G'GATC_C
Cuts at: 2319 4861 5228 2319
Size: 2542 367 4622
Fragments arranged by size:
4622 2542 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2338 2434 2529 2831 2975
Size: 325 217 627 551 96 95 302 144
Cuts at: 2975 3910 3968 3987 4852 6307 618
Size: 935 58 19 865 1455 1842
Fragments arranged by size:
1842 1455 935 865 627 551 325 302
217 144 96 95 58 19
BanII G_rGCy'C
Cuts at: 1391 2025 2032 2096 2161 2168 2391 3010 3178
Size: 634 7 64 65 7 223 619 168
Cuts at: 3178 3190 3894 4212 4475 4496 4683 4850 1391
Size: 12 704 318 263 21 187 167 4072
Fragments arranged by size:
4072 704 634 619 318 263 223 187
168 167 65 64 21 12 7 7
BbeI G_GCGC'C
Cuts at: 2835 3972 2835
Size: 1137 6394
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2615 3850 928
Size: 971 716 1235 4609
Fragments arranged by size:
4609 1235 971 716
BbvCI CC'TCA_GC
Cuts at: 4742 4742
Size: 7531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3981 4015 4234 4268 5722 5756 1903
Size: 34 2044 34 219 34 1454 34 3678
Fragments arranged by size:
3678 2044 1454 219 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5423 6633 5423
Size: 1210 6321
BclI T'GATC_A
Cuts at: 1548 4707 1548
Size: 3159 4372
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3807 4172
Size: 107 122 71 631 605 337 1798 365
Cuts at: 4172 6142 136
Size: 1970 1525
Fragments arranged by size:
1970 1798 1525 631 605 365 337 122
107 71
BglII A'GATC_T
Cuts at: 2907 3204 7526 2907
Size: 297 4322 2912
Fragments arranged by size:
4322 2912 297
BlpI GC'TnA_GC
Cuts at: 4265 4948 4265
Size: 683 6848
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2168 2330 2490 2532 3010 3190 3915
Size: 242 136 162 160 42 478 180 725
Cuts at: 3915 4037 4704 4737 5592 6748 1790
Size: 122 667 33 855 1156 2573
Fragments arranged by size:
2573 1156 855 725 667 478 242 180
162 160 136 122 42 33
BmgBI CAC'GTC
Cuts at: 1318 2613 2629 3370 3499 1318
Size: 1295 16 741 129 5350
Fragments arranged by size:
5350 1295 741 129 16
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4425 5262 6220
Size: 416 53 83 399 24 3008 837 958
Cuts at: 6220 442
Size: 1753
Fragments arranged by size:
3008 1753 958 837 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7531
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 4661 3836
Size: 825 6706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 3011 3059 3308 3995 4565 6191 1239
Size: 1211 561 48 249 687 570 1626 2579
Fragments arranged by size:
2579 1626 1211 687 570 561 249 48
Bpu10I CC'TnA_GC
Cuts at: 2095 4742 2095
Size: 2647 4884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3206 3788 4634 5656 6521 6819 3206
Size: 582 846 1022 865 298 3918
Fragments arranged by size:
3918 1022 865 846 582 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3807 6194 882
Size: 617 2308 2387 2219
Fragments arranged by size:
2387 2308 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2314 3241 3469 4342 5322 493
Size: 711 1110 927 228 873 980 2702
Fragments arranged by size:
2702 1110 980 927 873 711 228
BsaBI GATnn'nnATC
Cuts at: 7525 7525
Size: 7531
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 2274 2716 2832
Size: 53 83 186 936 618 123 442 116
Cuts at: 2832 3078 3969 4128 4566 5338 5720 6355 275
Size: 246 891 159 438 772 382 635 1451
Fragments arranged by size:
1451 936 891 772 635 618 442 438
382 246 186 159 123 116 83 53
BsaWI w'CCGG_w
Cuts at: 2193 2881 3831 4604 4611 5964 6362 6636 6783
Size: 688 950 773 7 1353 398 274 147
Cuts at: 6783 7194 2193
Size: 411 2530
Fragments arranged by size:
2530 1353 950 773 688 411 398 274
147 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2427 2457 3596
Size: 30 165 30 816 30 178 30 1139
Cuts at: 3596 3626 3887 3917 3971 4001 4082 4112 1178
Size: 30 261 30 54 30 81 30 4597
Fragments arranged by size:
4597 1139 816 261 178 165 81 54
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2611 2996 3134 3557 3604 3992 4310 4637
Size: 1425 385 138 423 47 388 318 327
Cuts at: 4637 4780 1186
Size: 143 3937
Fragments arranged by size:
3937 1425 423 388 385 327 318 143
138 47
BseYI C'CCAG_C
Cuts at: 2041 2089 2342 3086 3098 3957 4065 4463 4467
Size: 48 253 744 12 859 108 398 4
Cuts at: 4467 6734 7230 2041
Size: 2267 496 2342
Fragments arranged by size:
2342 2267 859 744 496 398 253 108
48 12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 3722 4203 4244 4307 4367 4685
Size: 573 714 1272 481 41 63 60 318
Cuts at: 4685 1163
Size: 4009
Fragments arranged by size:
4009 1272 714 573 481 318 63 60
41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 3310 4981 5742 6770 1540
Size: 275 186 18 1291 1671 761 1028 2301
Fragments arranged by size:
2301 1671 1291 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2330 3269 3726 4137 4737 5592 5677 6748 2330
Size: 939 457 411 600 855 85 1071 3113
Fragments arranged by size:
3113 1071 939 855 600 457 411 85
BsmI GAATG_Cn'
Cuts at: 5042 5135 5042
Size: 93 7438
BsmBI CGTCTCn'nnnn_
Cuts at: 3505 3505
Size: 7531
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2032 2096 2161 2168 2330 2391
Size: 399 235 7 64 65 7 162 61
Cuts at: 2391 2490 2532 3010 3178 3190 3269 3726 3894
Size: 99 42 478 168 12 79 457 168
Cuts at: 3894 3915 4037 4137 4212 4475 4496 4683 4704
Size: 21 122 100 75 263 21 187 21
Cuts at: 4704 4737 4850 5592 5677 6748 1391
Size: 33 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 457 399 263 235
187 168 168 162 122 113 100 99
85 79 75 65 64 61 42 33
21 21 21 12 7 7
BspEI T'CCGG_A
Cuts at: 2193 2881 3831 4604 4611 7194 2193
Size: 688 950 773 7 2583 2530
Fragments arranged by size:
2583 2530 950 773 688 7
BspHI T'CATG_A
Cuts at: 2293 5420 7150 2293
Size: 3127 1730 2674
Fragments arranged by size:
3127 2674 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2583 3436 3741 3825 4393 4657 4886 844
Size: 1739 853 305 84 568 264 229 3489
Fragments arranged by size:
3489 1739 853 568 305 264 229 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 3276 3664 4411 5418 1394
Size: 429 320 1133 388 747 1007 3507
Fragments arranged by size:
3507 1133 1007 747 429 388 320
BsrDI GCAATG_nn'
Cuts at: 65 1248 6026 6200 65
Size: 1183 4778 174 1396
Fragments arranged by size:
4778 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2258 2437 2636 2972 3048 3363 3411
Size: 110 448 179 199 336 76 315 48
Cuts at: 3411 3702 6175 6362 1700
Size: 291 2473 187 2869
Fragments arranged by size:
2869 2473 448 336 315 291 199 187
179 110 76 48
BsrGI T'GTAC_A
Cuts at: 96 3669 4572 96
Size: 3573 903 3055
Fragments arranged by size:
3573 3055 903
BssHII G'CGCG_C
Cuts at: 1925 2106 2108 2588 1925
Size: 181 2 480 6868
Fragments arranged by size:
6868 480 181 2
BssSI C'ACGA_G
Cuts at: 2329 4169 5591 6603 2329
Size: 1840 1422 1012 3257
Fragments arranged by size:
3257 1840 1422 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2505 794
Size: 1711 5820
BstBI TT'CG_AA
Cuts at: 6335 6335
Size: 7531
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7531
BstXI CCAn_nnnn'nTGG
Cuts at: 2525 3162 4159 4175 2525
Size: 637 997 16 5881
Fragments arranged by size:
5881 997 637 16
BstYI r'GATC_y
Cuts at: 1972 2319 2907 3204 4392 4861 5228 5613 5630
Size: 347 588 297 1188 469 367 385 17
Cuts at: 5630 7082 7168 7180 7526 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1188 588 469 385 367 347
346 297 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4728 4728
Size: 7531
BtgI C'CryG_G
Cuts at: 513 1065 2004 2685 2805 2850 3182 3588 513
Size: 552 939 681 120 45 332 406 4456
Fragments arranged by size:
4456 939 681 552 406 332 120 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3368 3957 487
Size: 683 56 2142 589 4061
Fragments arranged by size:
4061 2142 683 589 56
BtsI GCAGTG_nn'
Cuts at: 2368 2446 2506 2999 3711 4186 5047 5841 5861
Size: 78 60 493 712 475 861 794 20
Cuts at: 5861 2368
Size: 4038
Fragments arranged by size:
4038 861 794 712 493 475 78 60
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7531
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7496
DraI TTT'AAA
Cuts at: 4835 4835
Size: 7531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6538 809
Size: 507 5222 1802
Fragments arranged by size:
5222 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2256 2634
Size: 54 1425 50 275 18 186 240 378
Cuts at: 2634 2970 3031 3307 4017 4978 5867 7496 8
Size: 336 61 276 710 961 889 1629 43
Fragments arranged by size:
1629 1425 961 889 710 378 336 276
275 240 186 61 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 3307 4978 1537
Size: 275 204 1291 1671 4090
Fragments arranged by size:
4090 1671 1291 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 3257 3888 4287 5461 1353
Size: 1904 631 399 1174 3423
Fragments arranged by size:
3423 1904 1174 631 399
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2936 4811 6118 6492 6638 387
Size: 1064 138 1347 1875 1307 374 146 1280
Fragments arranged by size:
1875 1347 1307 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2450 2755 2924 3011 3059 3221
Size: 393 695 123 305 169 87 48 162
Cuts at: 3221 3308 3701 3995 4565 5594 6191 6978 7246
Size: 87 393 294 570 1029 597 787 268
Cuts at: 7246 1239
Size: 1524
Fragments arranged by size:
1524 1029 787 695 597 570 393 393
305 294 268 169 162 123 87 87
48
EcoO109I rG'GnC_Cy
Cuts at: 2028 2135 2165 2425 3025 3196 3292 3878 4090
Size: 107 30 260 600 171 96 586 212
Cuts at: 4090 4693 2028
Size: 603 4866
Fragments arranged by size:
4866 603 600 586 260 212 171 107
96 30
EcoRI G'AATT_C
Cuts at: 4840 5218 4840
Size: 378 7153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 7531
FspI TGC'GCA
Cuts at: 6037 6037
Size: 7531
HaeII r_GCGC'y
Cuts at: 1678 2444 2693 2835 2847 3972 6678 1678
Size: 766 249 142 12 1125 2706 2531
Fragments arranged by size:
2706 2531 1125 766 249 142 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4082 4114 6172 6204 6246
Size: 32 135 32 2510 32 2058 32 42
Cuts at: 6246 6278 1373
Size: 32 2626
Fragments arranged by size:
2626 2510 2058 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4875 5121 669
Size: 988 281 2937 246 3079
Fragments arranged by size:
3079 2937 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7531
HpaI GTT'AAC
Cuts at: 5121 5121
Size: 7531
KasI G'GCGC_C
Cuts at: 2831 3968 2831
Size: 1137 6394
KpnI G_GTAC'C
Cuts at: 3991 4856 3991
Size: 865 6666
MfeI C'AATT_G
Cuts at: 5130 5130
Size: 7531
MluI A'CGCG_T
Cuts at: 7407 7407
Size: 7531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 3615 4521 6645 6829 1305
Size: 957 1353 906 2124 184 2007
Fragments arranged by size:
2124 2007 1353 957 906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3033 4019 10
Size: 54 1425 343 1201 986 3522
Fragments arranged by size:
3522 1425 1201 986 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2523 3590 3692 4661 5489 5848
Size: 648 1167 190 1067 102 969 828 359
Cuts at: 5848 6007 518
Size: 159 2042
Fragments arranged by size:
2042 1167 1067 969 828 648 359 190
159 102
MspA1I CmG'CkG
Cuts at: 1606 2171 2687 2807 3184 3301 3340 3376 3762
Size: 565 516 120 377 117 39 36 386
Cuts at: 3762 4065 4083 4146 4467 4718 4947 5624 6772
Size: 303 18 63 321 251 229 677 1148
Cuts at: 6772 7017 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 565 516 386 377 321
303 251 245 229 120 117 63 39
36 18
NaeI GCC'GGC
Cuts at: 2260 2638 3050 3365 3704 2260
Size: 378 412 315 339 6087
Fragments arranged by size:
6087 412 378 339 315
NarI GG'CG_CC
Cuts at: 2832 3969 2832
Size: 1137 6394
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6040
Fragments arranged by size:
6040 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7531
NgoMIV G'CCGG_C
Cuts at: 2258 2636 3048 3363 3702 2258
Size: 378 412 315 339 6087
Fragments arranged by size:
6087 412 378 339 315
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7531
NotI GC'GGCC_GC
Cuts at: 4978 4978
Size: 7531
NspI r_CATG'y
Cuts at: 1132 1229 3676 4741 4889 6434 1132
Size: 97 2447 1065 148 1545 2229
Fragments arranged by size:
2447 2229 1545 1065 148 97
PasI CC'CwG_GG
Cuts at: 1348 4030 4621 4683 1348
Size: 2682 591 62 4196
Fragments arranged by size:
4196 2682 591 62
PciI A'CATG_T
Cuts at: 6430 6430
Size: 7531
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3343 1108
Size: 2235 5296
PfoI T'CCnGG_A
Cuts at: 2427 2888 2427
Size: 461 7070
PmeI GTTT'AAAC
Cuts at: 4835 4835
Size: 7531
PmlI CAC'GTG
Cuts at: 2314 2314
Size: 7531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2564 2596 4079 4111 5600 5632 7113 7145 2564
Size: 32 1483 32 1489 32 1481 32 2950
Fragments arranged by size:
2950 1489 1483 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 2425 3025 3196 3292 3878 4090 2135
Size: 290 600 171 96 586 212 5576
Fragments arranged by size:
5576 600 586 290 212 171 96
PshAI GACnn'nnGTC
Cuts at: 1613 3762 4050 4565 1613
Size: 2149 288 515 4579
Fragments arranged by size:
4579 2149 515 288
PsiI TTA'TAA
Cuts at: 5101 5101
Size: 7531
PspOMI G'GGCC_C
Cuts at: 2028 2164 3006 3186 2028
Size: 136 842 180 6373
Fragments arranged by size:
6373 842 180 136
PstI C_TGCA'G
Cuts at: 830 2081 2501 2606 3827 4359 4404 4883 830
Size: 1251 420 105 1221 532 45 479 3478
Fragments arranged by size:
3478 1251 1221 532 479 420 105 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7531
PvuII CAG'CTG
Cuts at: 3340 3762 4467 4718 3340
Size: 422 705 251 6153
Fragments arranged by size:
6153 705 422 251
SacII CC_GC'GG
Cuts at: 2688 2808 3185 2688
Size: 120 377 7034
Fragments arranged by size:
7034 377 120
SalI G'TCGA_C
Cuts at: 1936 4873 1936
Size: 2937 4594
SanDI GG'GwC_CC
Cuts at: 2135 2135
Size: 7531
SapI GCTCTTCn'nnn_
Cuts at: 3257 3257
Size: 7531
SbfI CC_TGCA'GG
Cuts at: 4883 4883
Size: 7531
ScaI AGT'ACT
Cuts at: 5779 7491 5779
Size: 1712 5819
SexAI A'CCwGG_T
Cuts at: 4508 4508
Size: 7531
SfcI C'TryA_G
Cuts at: 826 1045 2077 2361 2497 2602 3416 3823 4078
Size: 219 1032 284 136 105 814 407 255
Cuts at: 4078 4355 4400 4879 6014 6695 6886 826
Size: 277 45 479 1135 681 191 1471
Fragments arranged by size:
1471 1135 1032 814 681 479 407 284
277 255 219 191 136 105 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 3807 3807
Size: 7531
SfoI GGC'GCC
Cuts at: 2833 3970 2833
Size: 1137 6394
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7531
SgrAI Cr'CCGG_yG
Cuts at: 1700 2437 1700
Size: 737 6794
SmaI CCC'GGG
Cuts at: 1872 2026 2115 2163 2384 3136 3889 4698 4793
Size: 154 89 48 221 752 753 809 95
Cuts at: 4793 4858 1872
Size: 65 4545
Fragments arranged by size:
4545 809 753 752 221 154 95 89
65 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3221 3803 4649 5635 6536 6798
Size: 197 926 1278 582 846 986 901 262
Cuts at: 6798 820
Size: 1553
Fragments arranged by size:
1553 1278 986 926 901 846 582 262
197
SnaBI TAC'GTA
Cuts at: 493 5322 493
Size: 4829 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7531
SphI G_CATG'C
Cuts at: 1132 4889 1132
Size: 3757 3774
SrfI GCCC'GGGC
Cuts at: 4793 4793
Size: 7531
SspI AAT'ATT
Cuts at: 5 52 5455 5
Size: 47 5403 2081
Fragments arranged by size:
5403 2081 47
StuI AGG'CCT
Cuts at: 1517 3071 3161 3801 4443 1517
Size: 1554 90 640 642 4605
Fragments arranged by size:
4605 1554 642 640 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 3619 4048 4826 4970 513
Size: 552 939 1615 429 778 144 3074
Fragments arranged by size:
3074 1615 939 778 552 429 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2356 2490 2561 3949 5558 5575 5728
Size: 1255 776 134 71 1388 1609 17 153
Cuts at: 5728 5913 6358 325
Size: 185 445 1498
Fragments arranged by size:
1609 1498 1388 1255 776 445 185 153
134 71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3546 3669 4572
Size: 274 80 33 51 1443 1569 123 903
Cuts at: 4572 5777 7489 96
Size: 1205 1712 138
Fragments arranged by size:
1712 1569 1443 1205 903 274 138 123
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 2427 2459 3596 3628 3887 3919 7491
Size: 32 176 32 1137 32 259 32 3572
Cuts at: 7491 7523 2219
Size: 32 2227
Fragments arranged by size:
3572 2227 1137 259 176 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7531
XbaI T'CTAG_A
Cuts at: 4867 4867
Size: 7531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3586 4027 4589 3586
Size: 441 562 6528
Fragments arranged by size:
6528 562 441
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7531
XmaI C'CCGG_G
Cuts at: 1870 2024 2113 2161 2382 3134 3887 4696 4791
Size: 154 89 48 221 752 753 809 95
Cuts at: 4791 4856 1870
Size: 65 4545
Fragments arranged by size:
4545 809 753 752 221 154 95 89
65 48
XmnI GAAnn'nnTTC
Cuts at: 5660 5660
Size: 7531
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2275 5339 276
Size: 53 83 186 936 741 3064 2468
Fragments arranged by size:
3064 2468 936 741 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacI SwaI