Restriction map for
pFN21AB1290
(Circular) (Six-base) MAPSORT of: pFN21AB1290.seq Check: 3581 from: 1 to: 6952
pFN21AB1290 6952 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3262 3262
Size: 6952
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4762 278
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4295 1937
Size: 2358 4594
Acc65I G'GTAC_C
Cuts at: 4273 4273
Size: 6952
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3585 5015 6399 6667 1632
Size: 1953 1430 1384 268 1917
Fragments arranged by size:
1953 1917 1430 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6755
AflIII A'CryG_T
Cuts at: 1203 2188 3556 3979 5851 6828 1203
Size: 985 1368 423 1872 977 1327
Fragments arranged by size:
1872 1368 1327 985 977 423
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 2542 5681 2542
Size: 3139 3813
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2776 1184
Size: 1592 5360
AlwNI CAG_nnn'CTG
Cuts at: 2209 4695 6267 2209
Size: 2486 1572 2894
Fragments arranged by size:
2894 2486 1572
ApaI G_GGCC'C
Cuts at: 4203 4203
Size: 6952
ApaLI G'TGCA_C
Cuts at: 5009 6165 5009
Size: 1156 5796
ApoI r'AATT_y
Cuts at: 1493 2626 2803 4075 4152 4261 4487 4639 6748
Size: 1133 177 1272 77 109 226 152 2109
Cuts at: 6748 1493
Size: 1697
Fragments arranged by size:
2109 1697 1272 1133 226 177 152 109
77
AseI AT'TA_AT
Cuts at: 160 1032 5506 160
Size: 872 4474 1606
Fragments arranged by size:
4474 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 3474 3621 4277 1870
Size: 73 271 1260 147 656 4545
Fragments arranged by size:
4545 1260 656 271 147 73
BamHI G'GATC_C
Cuts at: 4282 4649 4282
Size: 367 6585
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2930 3419 4273 5728 618
Size: 325 217 627 1143 489 854 1455 1842
Fragments arranged by size:
1842 1455 1143 854 627 489 325 217
BanII G_rGCy'C
Cuts at: 1391 2513 2644 2849 3192 3901 4148 4203 4271
Size: 1122 131 205 343 709 247 55 68
Cuts at: 4271 1391
Size: 4072
Fragments arranged by size:
4072 1122 709 343 247 205 131 68
55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5143 5177 1903
Size: 34 3206 34 3678
Fragments arranged by size:
3678 3206 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4844 6054 4844
Size: 1210 5742
BclI T'GATC_A
Cuts at: 1548 3868 1548
Size: 2320 4632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 3617 5563
Size: 107 122 71 631 605 368 1577 1946
Cuts at: 5563 136
Size: 1525
Fragments arranged by size:
1946 1577 1525 631 605 368 122 107
71
BglII A'GATC_T
Cuts at: 6947 6947
Size: 6952
BlpI GC'TnA_GC
Cuts at: 2657 4369 2657
Size: 1712 5240
Bme1580I G_kGCm'C
Cuts at: 1790 4203 5013 6169 1790
Size: 2413 810 1156 2573
Fragments arranged by size:
2573 2413 1156 810
BmgBI CAC'GTC
Cuts at: 1318 2225 1318
Size: 907 6045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4683 5641 442
Size: 416 53 83 399 24 3266 958 1753
Fragments arranged by size:
3266 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2249 1056
Size: 1193 5759
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2516 3594 2516
Size: 1078 5874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2616 2766 3267 3285 3303 3321 3815
Size: 856 521 150 501 18 18 18 494
Cuts at: 3815 3927 5612 1239
Size: 112 1685 2579
Fragments arranged by size:
2579 1685 856 521 501 494 150 112
18 18 18
Bpu10I CC'TnA_GC
Cuts at: 3081 3081
Size: 6952
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 3833 5077 5942 6240 2420
Size: 1413 1244 865 298 3132
Fragments arranged by size:
3132 1413 1244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3370 5615 882
Size: 617 1871 2245 2219
Fragments arranged by size:
2245 2219 1871 617
BsaAI yAC'GTr
Cuts at: 493 1204 2189 4743 493
Size: 711 985 2554 2702
Fragments arranged by size:
2702 2554 985 711
BsaBI GATnn'nnATC
Cuts at: 6946 6946
Size: 6952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2696 4759 5141 5776
Size: 53 83 186 936 1163 2063 382 635
Cuts at: 5776 275
Size: 1451
Fragments arranged by size:
2063 1451 1163 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5385 5783 6057 6204 6615 5385
Size: 398 274 147 411 5722
Fragments arranged by size:
5722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3931 3961 1178
Size: 30 165 30 2528 30 4169
Fragments arranged by size:
4169 2528 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2369 2377 2393 2417 2593 3449 3520
Size: 1165 18 8 16 24 176 856 71
Cuts at: 3520 3602 3605 3647 3671 3695 3830 1186
Size: 82 3 42 24 24 135 4308
Fragments arranged by size:
4308 1165 856 176 135 82 71 42
24 24 24 18 16 8 3
BseYI C'CCAG_C
Cuts at: 3295 3313 3331 3355 6155 6651 3295
Size: 18 18 24 2800 496 3596
Fragments arranged by size:
3596 2800 496 24 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2547 2775 3909 1163
Size: 573 811 228 1134 4206
Fragments arranged by size:
4206 1134 811 573 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4402 5163 6191 1540
Size: 275 186 2401 761 1028 2301
Fragments arranged by size:
2401 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2644 3211 3901 4022 5013 5098 6169 2644
Size: 567 690 121 991 85 1071 3427
Fragments arranged by size:
3427 1071 991 690 567 121 85
BsmI GAATG_Cn'
Cuts at: 3394 4463 4556 3394
Size: 1069 93 5790
Fragments arranged by size:
5790 1069 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2513 2644 2849 3192 3211 3901 4022
Size: 399 723 131 205 343 19 690 121
Cuts at: 4022 4148 4203 4271 5013 5098 6169 1391
Size: 126 55 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 690 399 343 205
131 126 121 85 68 55 19
BspEI T'CCGG_A
Cuts at: 6615 6615
Size: 6952
BspHI T'CATG_A
Cuts at: 4841 6571 4841
Size: 1730 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3262 4307 844
Size: 2418 1045 3489
Fragments arranged by size:
3489 2418 1045
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 2359 3247 3661 3676 4839 1394
Size: 429 497 39 888 414 15 1163 3507
Fragments arranged by size:
3507 1163 888 497 429 414 39 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 5447 5621 65
Size: 1183 4199 174 1396
Fragments arranged by size:
4199 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 5596 5783 1700
Size: 110 389 3397 187 2869
Fragments arranged by size:
3397 2869 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 3935 96
Size: 3839 3113
BssHII G'CGCG_C
Cuts at: 1925 3691 1925
Size: 1766 5186
BssSI C'ACGA_G
Cuts at: 5012 6024 5012
Size: 1012 5940
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6952
BstBI TT'CG_AA
Cuts at: 2567 5756 2567
Size: 3189 3763
BstEII G'GTnAC_C
Cuts at: 1184 3985 1184
Size: 2801 4151
BstYI r'GATC_y
Cuts at: 1972 2530 3154 4106 4282 4649 5034 5051 6503
Size: 558 624 952 176 367 385 17 1452
Cuts at: 6503 6589 6601 6947 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 952 624 558 385 367 346
176 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2841 4209 2841
Size: 1368 5584
BtgI C'CryG_G
Cuts at: 513 1065 2004 2503 2663 3288 3360 513
Size: 552 939 499 160 625 72 4105
Fragments arranged by size:
4105 939 625 552 499 160 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6213
Fragments arranged by size:
6213 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 4468 5262 5282 2061
Size: 2407 794 20 3731
Fragments arranged by size:
3731 2407 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6917
DraI TTT'AAA
Cuts at: 4256 4256
Size: 6952
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5959 809
Size: 507 4643 1802
Fragments arranged by size:
4643 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2715 4399
Size: 54 1425 50 275 18 202 683 1684
Cuts at: 4399 5288 6917 8
Size: 889 1629 43
Fragments arranged by size:
1684 1629 1425 889 683 275 202 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4399 1537
Size: 275 2587 4090
Fragments arranged by size:
4090 2587 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 2310 2321 2611 2854 2921 3015 3237
Size: 921 36 11 290 243 67 94 222
Cuts at: 3237 3455 3513 3561 3774 4882 1353
Size: 218 58 48 213 1108 3423
Fragments arranged by size:
3423 1108 921 290 243 222 218 213
94 67 58 48 36 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2166 3101 3668 5539 5913
Size: 1064 138 435 142 935 567 1871 374
Cuts at: 5913 6059 387
Size: 146 1280
Fragments arranged by size:
1871 1280 1064 935 567 435 374 146
142 138
EcoICRI GAG'CTC
Cuts at: 2642 3899 2642
Size: 1257 5695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2616 2766 3267 3285 3303 3321
Size: 393 463 521 150 501 18 18 18
Cuts at: 3321 3585 3815 3927 5015 5612 6399 6667 1239
Size: 264 230 112 1088 597 787 268 1524
Fragments arranged by size:
1524 1088 787 597 521 501 463 393
268 264 230 150 112 18 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4208 4208
Size: 6952
EcoO109I rG'GnC_Cy
Cuts at: 4200 4200
Size: 6952
EcoRI G'AATT_C
Cuts at: 4261 4639 4261
Size: 378 6574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4211 4211
Size: 6952
FspI TGC'GCA
Cuts at: 5458 5458
Size: 6952
HaeII r_GCGC'y
Cuts at: 1678 3668 3793 6099 1678
Size: 1990 125 2306 2531
Fragments arranged by size:
2531 2306 1990 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2528 2560 2808 2840 3578
Size: 32 135 32 956 32 248 32 738
Cuts at: 3578 3610 4113 4145 5593 5625 5667 5699 1373
Size: 32 503 32 1448 32 42 32 2626
Fragments arranged by size:
2626 1448 956 738 503 248 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2683 4296 4542 669
Size: 988 281 745 1613 246 3079
Fragments arranged by size:
3079 1613 988 745 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6952
HpaI GTT'AAC
Cuts at: 2683 4542 2683
Size: 1859 5093
KpnI G_GTAC'C
Cuts at: 4277 4277
Size: 6952
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 6066 6250 1305
Size: 1008 3753 184 2007
Fragments arranged by size:
3753 2007 1008 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2717 10
Size: 54 1425 343 885 4245
Fragments arranged by size:
4245 1425 885 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2774 3931 4910 5269 5428 518
Size: 648 1608 1157 979 359 159 2042
Fragments arranged by size:
2042 1608 1157 979 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2735 3640 3646 4368 5045 6193 6438
Size: 600 529 905 6 722 677 1148 245
Cuts at: 6438 1606
Size: 2120
Fragments arranged by size:
2120 1148 905 722 677 600 529 245
6
NcoI C'CATG_G
Cuts at: 513 1065 2004 2503 2663 3288 3360 513
Size: 552 939 499 160 625 72 4105
Fragments arranged by size:
4105 939 625 552 499 160 72
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6952
NheI G'CTAG_C
Cuts at: 1052 2245 1052
Size: 1193 5759
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 3983 4310 5855 1132
Size: 97 2754 327 1545 2229
Fragments arranged by size:
2754 2229 1545 327 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6952
PciI A'CATG_T
Cuts at: 3979 5851 3979
Size: 1872 5080
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3269 1108
Size: 2161 4791
PfoI T'CCnGG_A
Cuts at: 3949 3949
Size: 6952
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 6534 6566 5021
Size: 32 1481 32 5407
Fragments arranged by size:
5407 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4091 4522 4091
Size: 431 6521
PspOMI G'GGCC_C
Cuts at: 4199 4199
Size: 6952
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3832 3864 4206 4238 3832
Size: 32 342 32 6546
Fragments arranged by size:
6546 342 32 32
PstI C_TGCA'G
Cuts at: 830 2655 3418 3645 4243 4304 830
Size: 1825 763 227 598 61 3478
Fragments arranged by size:
3478 1825 763 598 227 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
PvuII CAG'CTG
Cuts at: 2735 3640 2735
Size: 905 6047
SacI G_AGCT'C
Cuts at: 2644 3901 2644
Size: 1257 5695
SalI G'TCGA_C
Cuts at: 1936 4294 1936
Size: 2358 4594
SapI GCTCTTCn'nnn_
Cuts at: 2274 2310 2854 3237 3513 3774 2274
Size: 36 544 383 276 261 5452
Fragments arranged by size:
5452 544 383 276 261 36
SbfI CC_TGCA'GG
Cuts at: 4304 4304
Size: 6952
ScaI AGT'ACT
Cuts at: 5200 6912 5200
Size: 1712 5240
SfcI C'TryA_G
Cuts at: 826 1045 2651 3414 3641 3849 4239 4300 5435
Size: 219 1606 763 227 208 390 61 1135
Cuts at: 5435 6116 6307 826
Size: 681 191 1471
Fragments arranged by size:
1606 1471 1135 763 681 390 227 219
208 191 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6952
SmaI CCC'GGG
Cuts at: 1872 2216 3623 4279 1872
Size: 344 1407 656 4545
Fragments arranged by size:
4545 1407 656 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 3812 5056 5957 6219 820
Size: 197 926 456 1413 1244 901 262 1553
Fragments arranged by size:
1553 1413 1244 926 901 456 262 197
SnaBI TAC'GTA
Cuts at: 493 4743 493
Size: 4250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 4310 1132
Size: 3178 3774
SspI AAT'ATT
Cuts at: 5 52 4876 5
Size: 47 4824 2081
Fragments arranged by size:
4824 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6952
StyI C'CwwG_G
Cuts at: 513 1065 2004 2503 2663 3288 3360 3891 4391
Size: 552 939 499 160 625 72 531 500
Cuts at: 4391 513
Size: 3074
Fragments arranged by size:
3074 939 625 552 531 500 499 160
72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3046 3052 4979 4996 5149 5334 5779
Size: 1255 1466 6 1927 17 153 185 445
Cuts at: 5779 325
Size: 1498
Fragments arranged by size:
1927 1498 1466 1255 445 185 153 17
6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2911 3935 5198
Size: 274 80 33 51 1443 934 1024 1263
Cuts at: 5198 6910 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1263 1024 934 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2694 2726 3903 3935 6912 6944 2694
Size: 32 1177 32 2977 32 2702
Fragments arranged by size:
2977 2702 1177 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6952
XbaI T'CTAG_A
Cuts at: 2798 4288 2798
Size: 1490 5462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3358 3358
Size: 6952
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6952
XmaI C'CCGG_G
Cuts at: 1870 2214 3621 4277 1870
Size: 344 1407 656 4545
Fragments arranged by size:
4545 1407 656 344
XmnI GAAnn'nnTTC
Cuts at: 3767 5081 3767
Size: 1314 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4760 276
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI