Restriction map for
pFN21AB0997
(Circular) (Six-base) MAPSORT of: pFN21AB0997.seq Check: 8105 from: 1 to: 5524
pFN21AB0997 5524 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 3587 4971 5239 1632
Size: 807 1148 1384 268 1917
Fragments arranged by size:
1917 1384 1148 807 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 4423 5400 1203
Size: 3220 977 1327
Fragments arranged by size:
3220 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2190 2833 3059 3211 5320 1493
Size: 697 643 226 152 2109 1697
Fragments arranged by size:
2109 1697 697 643 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2081 2380 2849 1870
Size: 73 114 24 299 469 4545
Fragments arranged by size:
4545 469 299 114 73 24
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2806 2845 4300 618
Size: 325 217 627 1019 39 1455 1842
Fragments arranged by size:
1842 1455 1019 627 325 217 39
BanII G_rGCy'C
Cuts at: 1391 2057 2553 2843 1391
Size: 666 496 290 4072
Fragments arranged by size:
4072 666 496 290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4135 136
Size: 107 122 71 631 605 2463 1525
Fragments arranged by size:
2463 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2779 5519 2779
Size: 2740 2784
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 3585 4741 1790
Size: 1795 1156 2573
Fragments arranged by size:
2573 1795 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2516 3255 4213
Size: 416 53 83 399 24 1099 739 958
Cuts at: 4213 442
Size: 1753
Fragments arranged by size:
1753 1099 958 739 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 2760 4184 1239
Size: 1078 443 1424 2579
Fragments arranged by size:
2579 1424 1078 443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3649 4514 4812 3649
Size: 865 298 4361
Fragments arranged by size:
4361 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4187 882
Size: 617 2688 2219
Fragments arranged by size:
2688 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3331 3713 4348 275
Size: 53 83 186 936 1798 382 635 1451
Fragments arranged by size:
1798 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2235 3957 4355 4629 4776 5187 2235
Size: 1722 398 274 147 411 2572
Fragments arranged by size:
2572 1722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2290 2320 1178
Size: 30 165 30 887 30 4382
Fragments arranged by size:
4382 887 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2761 2764 1186
Size: 1575 3 3946
Fragments arranged by size:
3946 1575 3
BseYI C'CCAG_C
Cuts at: 2603 2615 4727 5223 2603
Size: 12 2112 496 2904
Fragments arranged by size:
2904 2112 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4951
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 3735 4763 1540
Size: 275 186 973 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 2553 3585 3670 4741 2097
Size: 456 1032 85 1071 2880
Fragments arranged by size:
2880 1071 1032 456 85
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
BsmBI CGTCTCn'nnnn_
Cuts at: 2237 2237
Size: 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2097 2553 2843 3585 3670 4741
Size: 399 267 40 456 290 742 85 1071
Cuts at: 4741 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 456 399 290 267 85
40
BspEI T'CCGG_A
Cuts at: 2235 5187 2235
Size: 2952 2572
BspHI T'CATG_A
Cuts at: 2223 3413 5143 2223
Size: 1190 1730 2604
Fragments arranged by size:
2604 1730 1190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 4019 4193 65
Size: 1183 901 1870 174 1396
Fragments arranged by size:
1870 1396 1183 901 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2785 4168 4355 1700
Size: 110 975 1383 187 2869
Fragments arranged by size:
2869 1383 975 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 2275 3584 4596 2275
Size: 1309 1012 3203
Fragments arranged by size:
3203 1309 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2779 2854 3221 3606 3623 5075 5161 5173
Size: 807 75 367 385 17 1452 86 12
Cuts at: 5173 5519 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 807 385 367 346 86 75
17 12
Bsu36I CC'TnA_GG
Cuts at: 2610 2610
Size: 5524
BtgI C'CryG_G
Cuts at: 513 1065 2048 2324 513
Size: 552 983 276 3713
Fragments arranged by size:
3713 983 552 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 2580 3040 3834 3854 2580
Size: 460 794 20 4250
Fragments arranged by size:
4250 794 460 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2347 2382 529
Size: 35 1783 35 3671
Fragments arranged by size:
3671 1783 35 35
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2697 2971 3860
Size: 54 1425 50 275 18 867 274 889
Cuts at: 3860 5489 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 867 275 274 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3454 1353
Size: 2101 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 2551
Size: 5524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2439 2760 3587 4184 4971 5239
Size: 393 685 122 321 827 597 787 268
Cuts at: 5239 1239
Size: 1524
Fragments arranged by size:
1524 827 787 685 597 393 321 268
122
EcoO109I rG'GnC_Cy
Cuts at: 2085 2320 2085
Size: 235 5289
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
EcoRV GAT'ATC
Cuts at: 2461 2461
Size: 5524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2211 2211
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2512 4671 1678
Size: 834 2159 2531
Fragments arranged by size:
2531 2159 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2332 2364 2435 2467 4165
Size: 32 135 32 760 32 71 32 1698
Cuts at: 4165 4197 4239 4271 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1698 760 135 71 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 4638 4822 1305
Size: 918 2415 184 2007
Fragments arranged by size:
2415 2007 918 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2699 10
Size: 54 1425 343 867 2835
Fragments arranged by size:
2835 1425 867 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3482 3841 4000 518
Size: 648 2316 359 159 2042
Fragments arranged by size:
2316 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2603 2940 3617 4765 5010 1606
Size: 444 553 337 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 553 444 337 245
NcoI C'CATG_G
Cuts at: 513 1065 2324 513
Size: 552 1259 3713
Fragments arranged by size:
3713 1259 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2375 2882 4427 1132
Size: 97 1146 507 1545 2229
Fragments arranged by size:
2229 1545 1146 507 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2144 2596 1108
Size: 1036 452 4036
Fragments arranged by size:
4036 1036 452
PfoI T'CCnGG_A
Cuts at: 2294 2294
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2085 2320 2085
Size: 235 5289
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
PvuII CAG'CTG
Cuts at: 2603 2603
Size: 5524
SacI G_AGCT'C
Cuts at: 2553 2553
Size: 5524
SacII CC_GC'GG
Cuts at: 2051 2051
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SanDI GG'GwC_CC
Cuts at: 2085 2085
Size: 5524
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SfcI C'TryA_G
Cuts at: 826 1045 2872 4007 4688 4879 826
Size: 219 1827 1135 681 191 1471
Fragments arranged by size:
1827 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2083 2851 1872
Size: 211 768 4545
Fragments arranged by size:
4545 768 211
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3628 4529 4791 820
Size: 197 926 1685 901 262 1553
Fragments arranged by size:
1685 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 2438 1517
Size: 921 4603
StyI C'CwwG_G
Cuts at: 513 1065 2324 2963 513
Size: 552 1259 639 3074
Fragments arranged by size:
3074 1259 639 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3551 3568 3721 3906 4351 325
Size: 1255 1971 17 153 185 445 1498
Fragments arranged by size:
1971 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3770 5482 96
Size: 274 80 33 51 1443 1793 1712 138
Fragments arranged by size:
1793 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2290 2322 5484 5516 2290
Size: 32 3162 32 2298
Fragments arranged by size:
3162 2298 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2081 2849 1870
Size: 211 768 4545
Fragments arranged by size:
4545 768 211
XmnI GAAnn'nnTTC
Cuts at: 2194 3653 2194
Size: 1459 4065
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI XcmI